GDR What’s New and What’s Next Dorrie Main, Sook Jung, Stephen Ficklin, Taein Lee, Chun-Huai Cheng, Anna Blenda, Jing Yu, Ping Zheng, Sushan Ru, Julia Piaskowski, Cameron Peace, Kate Evans, Mercy Olmstead, Ksenjia Gasic, Lisa Wasko DeVetter, Nnadozie Oraguzie, Albert Abbott Fruit and Nuts Workshop PAG XXIII, San Diego CA. Jan 10, 2015.
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GDR What’s New and What’s Next Dorrie Main, Sook Jung, Stephen Ficklin, Taein Lee, Chun-Huai Cheng, Anna Blenda, Jing Yu, Ping Zheng, Sushan Ru, Julia.
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GDRWhat’s New and What’s Next
Dorrie Main, Sook Jung, Stephen Ficklin, Taein Lee, Chun-Huai Cheng, Anna Blenda, Jing Yu, Ping Zheng, Sushan Ru, Julia Piaskowski, Cameron Peace, Kate Evans, Mercy Olmstead,
Ksenjia Gasic, Lisa Wasko DeVetter, Nnadozie Oraguzie, Albert Abbott
Fruit and Nuts WorkshopPAG XXIII, San Diego CA. Jan 10, 2015.
Applied ScienceUtilization of DNA information in breeding decisions
Integrated Data Facilitates Discovery
Vision for GDR
• To provide high-quality online community database resources to enable Rosaceae research in genomics, genetics and breeding• Easy to use• Current• Scalable and fast• Easy to manage and update• Resource efficient • Utilize a common, interoperable platform• Interoperable with other useful databases• Self-sustaining in the medium term
Rosaceae Researcher without an up to date,
comprehensive database
Button-clicking energized RR using an up to datedatabase to help make research-decisions
Current Data and Functionality• Data for all major crops - almond, apple, apricot, blackberry, cherry,
peach, pear, raspberry, rose, strawberry• Annotated peach, strawberry, pear and apple genome sequences such
as gene models, mapped transcripts, putative gene function assignments – GO, KEGG)
• Apple-peach-strawberry synteny available through GBrowse_Syn• Curated Rosaceae gene database• Annotated genera and family unigenes (v5)• Pathway data (PeachCyc, FragariaCyc and AppleCyc)• 9K apple, 9K peach and 6K cherry illumina array SNPs• 123 Genetic maps • Gene, EST, marker, trait, QTL, polymorphism, publications search
modules• Genotypic, phenotypic and breeding data for search and download• Decision tools for breeders• BLAST, GenSAS, CAP3, SSR, Sequence Retrieval online tools
Major Data Sets in GDR
Accessing GDR
Community Databases and Big Data
Recent advances in sequencing, genotyping, and phenotyping technologies have led to a paradigm shift in crop science research – driven by “Big Data” Individual scientists now routinely
• Sequence and genotype genomes from populations, families, individuals of interest
• Pursue large-scale gene expression studies
• Create highly saturated genetic maps
• Identify genome wide loci influencing traits of interest• Conduct large-scale standardized phenotyping.
GDR Funding
• 2003-2014: Funded by NSF PGRP, USDA NIFA SCRI, WTFRC, Washington State University, & Clemson University
• More formalized structure has been implemented to ensure the community needs are met
• The U.S. Rosaceae Genomic, Genetics and Breeding Executive Committee (RosEXEC) will be the official Advisory Board to GDR
• Quarterly reports will be submitted and quarterly GoToMeetings held to receive input and feedback
What’s Coming – More specifically
• Add more large-scale data (genomic, transcriptomes, phenotypic, genotypic)• >1000 apple accessions - GBS data• >200 apricot genomes• Large scale phenotypic and genotypic data from
RosBREED 2 and FruitBreedOmics• RNASeq data from multiple projects• Raspberry and rose reference genomes
What’s Coming – GenSAS v3.0
What’s Coming – More specifically• Add more curated QTL and trait data, annotated by
standardized community agreed ontologies
• Further incorporate and develop online tools to aid Marker-Assisted Breeding such as field apps, expanded public and private breeders toolbox.
• Make the current and future Tripal modules publicly available
• Further refinement/development of the Tripal modules– QTL, germplasm and diversity module– Breeders Toolbox– Web services
GDR Specific Activities at PAG
• Talk: Fruit and Nut Workshop Saturday at 8.00 - 8.15 am
• Poster P0888 Monday at 10 - 11.30 am
• Computer Demo Monday at 2.30 - 2.45 pm
• Hands on Help Plant Database Booth: Sunday at 7.20 - 7.40 am,
Monday at 10.20 - 10.40 am and 3.30 - 3.40 pm
• RosEXEC Meeting on Sunday: 8.00 – 10.00 am
Other GDR Related Activities at PAGGenSAS
• Pine Workshop TalkSaturday at 10.30 – 11.00 am
• Computer Demo Saturday at 2.30 - 2.45 pm
• Poster P1153Monday at 3.00 - 4.30 pm
Tripal Workshop (Saturday 4.00 – 5.40 pm)• Funded Tripal Development 4.10 – 4.30 pm• Converting GDR/CottonGen to Tripal 4.30 pm)
Pine Genome Sequencing Project; CottonGen, Genome Database for Vaccinium; Cool Season Food Legume Database;
• Rosaceae, Citrus, Cacao, Blueberry, Pea, Chickpea, Lentil, Cotton and Bioinformatics Community
• Funding Sources:USDA NIFA, NSF Plant Genome Program, USDA-ARS, Washington Tree Fruit Research Commission, Mars Inc, USA Dry Pea and Lentil Commission, Cotton Industry, Citrus Industry, Northern Pulse Growers Washington State University, Clemson University, and University of Florida