Gas Chromatography (Chapter 2) Two major types • Gas-solid chromatography (stationary phase: solid) • Gas-liquid chromatography (stationary phase: immobilized liquid) Retention Volume : V R = t R ⋅ F retained 1 2 4 3 4 V M = t M ⋅ F non - retained 1 2 4 3 4 average volumetric flow rate (mL/min) F can be estimated by measuring flow rate exiting the column using soap bubble meter (some gases dissolving in soap solution) but measured V R and V M depend on • pressure inside column • temperature of column MKI 211 page 2.1
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Gas Chromatography (Chapter 2)Gas Chromatography (Chapter 2) Two major types • Gas-solid chromatography (stationary phase: solid ) • Gas-liquid chromatography (stationary phase:
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Gas Chromatography (Chapter 2)
Two major types
• Gas-solid chromatography
(stationary phase: solid)
• Gas-liquid chromatography
(stationary phase: immobilized liquid)
Retention Volume:
VR = tR ⋅ F
retained1 2 4 3 4 VM = tM ⋅F
non−retained1 2 4 3 4
average volumetric flow rate (mL/min)
F can be estimated by measuring flow rate exiting the column usingsoap bubble meter (some gases dissolving in soap solution)
but measured VR and VM depend on
• pressure inside column
• temperature of column
MKI 211 page 2.1
Bambang Yudono
Yudono's Lecture Note
VR and VM depend on average pressure inside column
Column has resistance to flow
At inlet , P = high, F = low
At outlet, P = low, F = high
P ⋅ F = constant
Pressure drop factor j used to calculate average pressure from inletpressure Pinlet and outlet pressure Poutlet