G9 - Banana Tissue Culture - Genewinbiotech

GRAND NAINE-(G-9)PLANTATION GUIDE

- THE MOST ECONOMICAL PLANT PRODUCED BY GENEWIN BIOTECH,HOSUR, TAMIL NADU, INDIA

2012

Dr.V.Palani, P.Manasa,M.TechGENEWIN BIOTECH

1/10/2012

GRAND NAINE-(G-9) 2

GENEWIN BIOTECH | www.genewinbiotech.comContact us: [email protected],

[email protected]

2

qwertyuiopasdfghjklzxcvbnmqwertyuiopasdfghjklzxcvbnmqwertyuiopasdfghjklzxcvbnmqwertyuiopasdfghjklzxcvbnmqwertyuiopasdfghjklzxcvbnmqwertyuiopasdfghjklzxcvbnmqwertyuiopasdfghjklzxcvbnmqwertyuiopasdfghjklzxcvbnmqwertyuiopasdfghjklzxcvbnmqwertyuiopasdfghjklzxcvbnmqwertyuiopasdfghjklzxcvbnmqwertyuiopasdfghjklzxcvbnmqwertyuiopasdfghjklzxcvbnmqwertyuiopasdfghjklzxcvbnmqwertyuiopasdfghjklzxcvbnmqwertyuiopasdfghjklzxcvbnmqwertyuiopasdfghjklzxcvbnmqwertyuiopasdfghjklzxcvbnmrtyuiopasdfghjklzxcvbnmqwertyuiopasdfghjklzxcvbnmqwertyuiopasdfghjklzxcvbnmqwertyuiopasdfghjklzxcvbnmqw

GRAND NAINE-(G-9)PLANTATION GUIDE1/10/2012

Dr.V.Palani, P.Manasa,M.Tech

www.genewinbiotech.com

mailto:[email protected]


GRAND NAINE-(G-9) 3


[email protected]

3

PLANTATION GUIDE FOR GRAND NAINE- THE MOST ECONOMICAL

PLANT

Tissue cultured Saplings are to be grown with utmost care. These Banana

plants are suitable for plantation throughout the year unlike the other plants that

are seasonal. In favor of the farmers, Genewin Biotech-Quality producers of

Tissue cultured plants located in Hosur, TamilNadu, India produces these healthy

disease free Grand Naine saplings of about 5 Million every year and supply them

throughout the India and world countries.

Soil:

Land with good drainage facility can be chosen for the cultivation of banana

saplings. Red soil and other moderate soil with less amount of sand proportion will

be suitable soil for banana plants. The pH of the soil should be neutral or between

5.5 -7.5. Thus the soil should be subjected to the soil test in a suitable laboratory

or government based horticulture labs to confirm the pH of soil. If the soil is more

acidic, it is to be neutralized using the gypsum and if the soil is more basic,

neutralize it with Dolamite. This neutralization is very important to make the soil

suitable for the cultivation.

Planting season:

Tissue cultured plants are not season dependent. They can be planted to the

soil throughout the year. The Major difference between the normal and the tissue




GRAND NAINE-(G-9) 4


[email protected]

4

cultured plants is that Tissue cultured plants will have twice the amount of roots

that are present in the normal plants. This unique property gives the plant the

strength and good nutrient absorbing capacity thus gives good yield to a average

of 50 kg/ bunch.

Making the land ready for plantation:

Plough the land 3-4 times evenly. Before one month of planting period, dig

the land with small 2x2x2 ft pits with a intermediate distance of about 6x6 ft and

allow it to dry. For one acre, 1210 plants can be planted. In those pits add

cowdung : surface soil-1:1, neem cake-200gm,furadon-10gm and Rock Phosphate-

100gm and mix well. By doing this, Nematodes can be eliminated from the soil that

may attack the sapling when planted.

Planting of Saplings in field

Tear the polythene bags carefully using a sharp blade. Care must be taken

not to damage the roots inside the bag that may create shock to the plants and can

cause death of plant. While planting measure the height of the polybag and

accordingly plant the sapling. Both the planting pit and polybag height should be

same. This will reduce the mortality rate of the plant.

Conventional irrigation Vs Drip irrigation

Immediate irrigation of water is very important after planting. Once in every

week 3-4 times water to be supplied to plantations. Around each plant 1.5 ft soil to

be digged to retain the water for the plant consumption. The whole digged areas




GRAND NAINE-(G-9) 5


[email protected]

5

should be flooded with water to maintain the good health in plants. Care must be

taken not to allow the surface soil to get completely dried always. Moisture content

should be always present in the soil. Every plant should be irrigated with 16-20

lts/plant/day. Make a small draining way between each plant to avoid stagnancy of

water which may cause disease in plants.

DRIP IRRIGATION

Drip irrigation is to be planned before plantation is carried out. According

to the distance between each plant, drip irrigation set up is constructed to save

water from evaporation and excess draining. By this method, we can even avoid

weeds. Weed removal is a complicated and labor dependent process which is

completely eliminated in this process. Drip irrigation saves 56% of water and 23-

32% of productivity can be increased as per agricultural statistics.

Table for Drip irrigation:

S.No Plant

Age

Weeks Fertilizer Fertilizer

Amt in

kg/acre/week

Total

fertilizer

quantity/acre

1 1-8 8 19:19:19+

Calcium nitrate

2

16.25

16

130

2 9-16 8 12:61:00+

Calcium nitrate

6

20.75

48

166

3 17-26 10 12:61:00+ 0.5 5




GRAND NAINE-(G-9) 6


[email protected]

6

13:00:45+

Calcium nitrate

5

3

50

30

4 27-40 14 13:00:45+

00:00:50

2

4.5

28

63

5 41-42 2 00:00:50 5 10

CONVENTIONAL IRRIGATION:

As tissue cultured plants have twice amount of roots than normal plants, it

uptakes all the nutrients that is given to the plant in the form of fertilizer irrigation.

Fertilizer requirement is based on the type of soil where the plantation is carried

out. It should be tested and the guidance of the horticulture professional is taken.

Generally, average amount of fertilizer requirement is 200:90:300 NPK gm/plant.

The above requirement is divided and given to the plant as in the below statement.

For the first and second application stage, dig the soil around the plant but

20 cm away from the plant to irrigate the fertilizer. For third and fourth

application stage, make sure to dig the soil 50 cm away from the plant for

irrigation. For the last fifth and sixth application stage, dig the soil 75 cm away

from the plant and irrigate the fertilizer as tabulated below:




GRAND NAINE-(G-9) 7


[email protected]

7

Application

Stage

Day of

application

Urea

Fertilizer

amt in

gms/plant

DAP Potash Neem

Cake

1 30th day 35 40 60 10

2 75thday 75 40 95 10

3 125thday 90 40 110 10

4 165thday 90 40 110 20

5 210thday 65 40 100 20

6 After

Flowering

45 40 90 20

Azospirillum and Phosphobacteria can also be used as a fertilizer but it

should not mixed with other fertilizers in fact. Both azospirillum and

phosphobacteria of about 6 kg/ acre in 3rd month and 5th month is to be applied to

the plants. Vermicompost 500gm/plant is recommended for the good growth of

the plants. Addition of Microfood available in market will enhance growth of

plants.




GRAND NAINE-(G-9) 8


[email protected]

8

Note:

Plant in the evening time in the moist soil to avoid initial mortality

Apply 0.1% Emison or 01.% Bagallol or bleaching powder 10gm/litre of

water after planting. Repeat this on 3rd and 5th month after planting.

Spray cytozyme on 90th,150th and 210th day after planting 250 ml in 250

litres of water for getting uniform growth and higher yield.

Additional Practices:

1. Weed management: Weeds can be removed using spade or using the

recommended weedicides viz., Diuron 1.2 kg per acre in 500 litres water for

initial 1 to 2 months. Spray post emergence weedicide

Glyphosphate@8ml/litre of water when the weeds are at 2-3”height stage.

Care must be taken not to spray on banana leaves.

2. Irrigate the field regularly depending on soil moisture and climate

conditions.

3. Removal of side suckers: From 3rd month after planting, remove side suckers

once a month without damaging the mother plant.

4. Remove the dried leaves periodically. Do not cut green leaves.

5. Earthing up during 3rd and 7th month after planting.

6. Propping: Supporting the plants can be done using suitable propping

materials.

7. Intercrops: Onion, Soyabean, Cowpea can be grown as intercrop which will

give an additional income. Also these intercrops suppress the weed growth.




mailto:Glyphosphate@8ml

GRAND NAINE-(G-9) 9


[email protected]

9

Major Pests and Diseases of Banana:

Sl.No Pests & Diseases Symptoms Control measures

I PESTS:

1.Aphids

Acts as a vector of bunchy

top virus. It sucks the sap by

congregating under the

lower leaf base of

pseudostem or crown of the

plant. The infested leaves dry

up quickly and fall from

plant

Spray Monocrotophos

500ml/acre.

2. Nematode The infested plants show

stunted growth, having small

leaves and galled roots,

bunch choking and poor

bunch qualities. In severe

cases the infested plants

topple easily

Apply Carbofuron 3G at

the rate of 20 gm/plant at

the time of planting, 30gm

each on 3rdand 5th month

after planting.

II DISEASES:

1.Leaf spot disease

Appears Brown colour

spindle (eye shaped) spots &

drying of leaves

Spray Carbendazim at the

rate of 250 gm




GRAND NAINE-(G-9) 10


[email protected]

10

2. Wilt Disease Leaf colour change to yellow

and leaves bends downward.

Central leaves will be

remaining green

Fill 40 mg of Carbendazim

in a capsule & make a hole

in 45˚angle in the

Rhizome. Keep the capsule

inside the hole, close it with

clay soil.

III VIRAL DISEASES:

1. Bunchy top

Intermittent dark green

streaks along the veins,

midribs, leaf size gets

reduced and shrinked,

resetting of reduced leaves at

the top.

Remove the infested Plant

and burn. Disease spread

by Aphid. So avoid

intercrops in which Aphid

population and virus

symptom are usually more.

(eg:Cucurbitaceae family,

Bhendi & Tomato).

2.Other Mosaic

diseases

Leaves are brittle, infection

after bunch emergence

reduces bunch size.

Prophylactic spray of Neem

gold or Dimethoate 250

ml/acre.




G9 - Banana Tissue Culture - Genewinbiotech

Technology

ph of soil

surface soil

soil test

moderate soil

red soil

soil thatmay

normal plants

ft soil tobe