FYP Workshop Seminar presentation

Final Year Project – Seminar Presentation

Azizan Mohd. NoorUniKL MICET

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Why do I need to do well in Presentations?

• Phase 1 – 35%• Phase 2 – 30% • Approx. 32.5% of 6 credits; equivalent to 1.95 credits of the

total marks in the assessment of the project (6 credits) comes from Presentations.

• Presentation is only for 30 min each

• For a normal course 1.95 credits would be equivalent to approximately 18 h of lectures or 336 h of lab. time; in 14 weeks!

• WORTH YOUR WHILE TO DO WELL!2

Prerequisites for a good seminar presentation

• Good researchGood research• Good understanding of your researchGood understanding of your research• Well prepared materialsWell prepared materials

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Preparation of seminar materials• Suitable for the time allocatedSuitable for the time allocated ie. ie. 2020

min min presentationpresentation andand 1010 min min Q&AQ&A

• Suitable medium for presentation – Suitable medium for presentation – Powerpoint being the usual choicePowerpoint being the usual choice

• PPrecise and conciserecise and concise

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Content of a seminar presentation

• IntroductionIntroduction• ObjeObjecctitive(s)ve(s)• Materials and methodsMaterials and methods• Results and discussionResults and discussion• ConclusionConclusion

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Introduction

• A brief description of the background A brief description of the background of the researchof the research

• Current status of knowledge in the Current status of knowledge in the area of researcharea of research

• Importance of the researchImportance of the research

Intro1 6

An intracellular storage material An intracellular storage material

accumulated by a wide variety accumulated by a wide variety of microorganismsof microorganisms

O C (CH(CH2))nn C

O100-30 000

R

Poly(3-hydroxyalkanoate) PHA

Intro27

PHA can be divided into:

• Short-chain-length PHA (scl-PHA) eg: 3-hydroxypropionate, 3-hydroxybutyrate, 4-hydroxybutyrate, 3-hydroxyvalerate

• Medium-chain-length (mcl-PHA) eg: 3-hydroxyhexanoate, 3-hydrooctanoate, 3-hydroxydecanoate

Intro3 8

The type of PHAs produced depends on:

• Bacteria strains• Carbon sources• Culture conditions

Intro4 9

Some uses of PHAs

• Degradable packaging for consumer products eg. bottles, bags, films

• Drug-release matrix devices

• Starting material for the synthesis of enantiomeric pure chemicals

• Paint industry – as a binder

Intro5 10

• To screen for local PHA producers

• To optimize the culture conditions

• To manipulate the molecular weight of the polymer(s)

Objectives

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Materials and methods

• Main methods used• Concise – use diagrams or flow-charts if

possible

Materials n methods 1 12

ProcedureSoil samples (1 g) obtained from various locations were suspended in 10 ml of sterile distilled water. The suspensions were then plated out on nutrient agar and incubated at room temperature. Colonies producing biopolymer were identified by the flourescent orange colour formed upon flooding with Nile Blue A.

To assess the efficacy of PHA production by the various isolates, the colonies were grown in semi-synthetic liquid medium with glucose as the C-source. The cultures were incubated aerobically for 7 days after which the PHA produced were determined using a gas-liquid chromatograph.

Materials n methods 213

Procedure

Soil samples (1 g) obtained from various locations were suspended in 10 ml of sterile distilled water. The suspensions were then plated out on nutrient agar and incubated at room temperature. Colonies producing biopolymer were identified by the flourescent orange colour formed upon flooding with Nile Blue A.

To assess the efficacy of PHA production by the various isolates, the colonies were grown in semi-synthetic liquid medium with glucose as the C-source. The cultures were incubated aerobically for 7 days after which the PHA produced were determined using a gas-liquid chromatograph.

Materials n methods 3 14

Screening

Growth of USM4-55 in specific mediumGrowth of USM4-55 in specific medium

Aerobic fermentationAerobic fermentation(shake flask)(shake flask)

Cell harvestingCell harvesting

Cell drying (freeze-drying)Cell drying (freeze-drying)

GCGC polymer extractionpolymer extraction

Molecular weight analysis

Procedure

Materials n methods 415

Results

• Very improtant

• Concise

• Arranged according to the objectives of the seminar

• Table, graph or photo?

Results 1 16

C/N (mol/mol)

Cell dry

weight (g/l)

Polyester content (wt%)

Polyester composition3C4 3C6 3C10 3C8 3C12 3C12:1 3C14

10 4.50 25 22 2 24 26 15 2 10

15 3.88 26 23 2 24 25 10 1 14

20 2.92 37 14 1 31 32 9 1 12

30 1.60 43 19 1 24 30 12 1 13

40 1.25 50 8 1 28 32 12 1 18

 3C4 - 3-hydroxybutyrate, 3C6 - 3-hydroxyhexanoate, 3C8 - 3-hydroxyoctanoate, 3C10 - 3-hydroxydecanoate, 3C12 - 3-hydroxydodecanoate, 3C12:1 - 3-hydrododecenoate, 3C14 - 3-hydroxytetradecanoate

Effect of C/N molar ratio on production of polymer from oleic acid by USM4-55

Results 2 17

C/N (mol/mol)

Cell dry

weight (g/l)

Polyester content (wt%)

Polyester composition3C4 3C6 3C10 3C8 3C12 3C12:1 3C14

10 4.50 25 22 2 24 26 15 2 10

15 3.88 26 23 2 24 25 10 1 14

20 2.92 37 14 1 31 32 9 1 12

30 1.60 43 19 1 24 30 12 1 13

40 1.25 50 8 1 28 32 12 1 18

 3C4 - 3-hydroxybutyrate, 3C6 - 3-hydroxyhexanoate, 3C8 - 3-hydroxyoctanoate, 3C10 - 3-hydroxydecanoate, 3C12 - 3-hydroxydodecanoate, 3C12:1 - 3-hydrododecenoate, 3C14 - 3-hydroxytetradecanoate

Effect of C/N molar ratio on production of polymer from oleic acid by USM4-55

Results 3 18

Polymer molecular weight profile

0 20 40 60 800.0

0.2

0.4

0.6

0.8

1.0

1.2

1.4

P(3HB) Mw

P(3

HB

) Mw

, (1x

106 )

Time (hour)

0.2

0.3

0.4

0.5

0.6

0.7

0.8

mcl

-P(3

HA

) Mw

, (1x

106 )

mcl-(P3HA) Mw

Results 4 19

Optimisation of glucoamylase production by Aspergillus niger

0 1 2 3 4 5 6 7 8 9 10 11 12

Time (days)

0

10

20

30

40

50

Glucoamylase activity (U/ml)

Initial1.0% Urea0.3% YE5.0% RLGSpHTemp.

Results 5 20

Microorganism

• Pseudomonas sp. USM4-55 was locally isolated from the soil. It was identified by using API20E.

• Ability to produce biopolymer detected using Nile-Blue-A staining method and gas chromatographic analysis.

Results 621

mcl-P(3HA) P(3HB)

Results 7 22

Conclusion• GC is still the better method for screening of new PHA

producers

• USM4-55 produces both scl- and mcl-PHA

• USM4-55 can use both glucose and oleic acid to produce PHA

• Polymer accumulation is highly stimulated by nitrogen and oxygen limitation

• Polymer molecular weight can be manipulated by adding certain chemical compounds

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Effective seminar presentation• Have a good understanding of the

research carried out• Confidence and poise• Good English• Address the audience• Adhere to the time allocated• Practise!!

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