Fundamentals of Nanotechnology: Relationship to Food Science and Technology Tom Huang, Winnie Chen, Tao Geng, Rafael Gomez, Rashid Bashir, Arun Bhunia and Michael R. Ladisch Department of Agricultural and Biological Engineering LORRE Purdue University
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Fundamentals of Nanotechnology:Relationship to
Food Science and Technology
Tom Huang, Winnie Chen, Tao Geng, Rafael Gomez, Rashid Bashir,
Arun Bhunia and Michael R. Ladisch
Department of Agricultural and Biological EngineeringLORRE
Purdue University
Acknowledgements
Research supported by USDA ARS Contract 1935-42000-035 National Defense University (DAB J29-03-P-0022)
Richard Linton, Director, Center of Food Safety Engineering
Randy Woodson Director and Acting Dean,
School of Agriculture
Nanoscience and Nanotechnology
Nanoscience:Fabrication, study and modeling of devices and structures where at least one dimension is 200 nm or smaller.
Nanotechnology:Enables devices that are compact, portable, energy efficient, integrate sensing, and carry out complex functions of a full-scale laboratory
Background: Microfluidics
Movement of fluids at microscopic level Micron-sized channels and featuresApplications
BiosensorsMicro-bioseparationsPathogen detection
Benefits: Microfluidic Systems
MiniaturizationConsumes less reagentsEnables higher sensitivityShorter analysis time
Rapid Prototyping using PDMS UV
High resolution transparency as maskphotoresistSiO2 or silicone substrate
Develop photoresist obtains master
Pour PDMS over master Cure 70 °C for 1 hour
PDMS
PDMS Release PDMS from master and seal against a flat substrate
Microchannels formed. PDMS
Process takes a day or more
The Need
Simple and rapid fabricationWell defined surface chemistries Flexibility and adaptabilityAmendable for rapid prototyping
techniques
Microfiber Assisted Fabrication in 15 minFlat PDMS cover
Glass fiber (~12 µm diameter)
PDMS
SiO2
SiO2, glass, or PDMS substrate
1 µL labeled avidin (green)
Glass fiber
t = 3 minGlass substrateSiO2 substrate
(a) (b) (c)
Flow channel
1 nL/mm channel
Mixing in Nanoliter Well
Glass fiber (~12 µm dia)PDMS
(a)SiO2 substrate
1 nL well at intersectionLabeled BSA (red, 200 µg/ml)