Fundamentals of Nanotechnology for Agriculture Michael Ladisch 1 , Tom Huang 1 , Nathan Mosier 1 , and Robert Armstrong 2 1 Department of Agricultural and Biological Engineering LORRE Purdue University and 2 Center for Strategic Studies National Defense University 229 th ACS Meeting, San Diego, CA, Paper 205, March 17, 2005
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Fundamentals of Nanotechnology for Agriculture · 2013. 8. 23. · Fundamentals of Nanotechnology for Agriculture Michael Ladisch1, Tom Huang1, Nathan Mosier1, and Robert Armstrong2
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Fundamentals of Nanotechnologyfor Agriculture
Michael Ladisch1, Tom Huang1, Nathan Mosier1, and Robert Armstrong2
1Department of Agricultural and Biological EngineeringLORRE
Purdue Universityand
2Center for Strategic StudiesNational Defense University
229th ACS Meeting, San Diego, CA, Paper 205, March 17, 2005
Acknowledgements
Research supported by USDA ARS Contract 1935-42000-035 National Defense University (DAB J29-03-P-0022)
Richard Linton, Director, Center of Food Safety Engineering
Randy Woodson Dean, School of Agriculture
Nanoscience and Nanotechnology
Nanoscience:Fabrication, study and modeling of devices and structures where at least one dimension is 200 nm or smaller.
Nanotechnology:Enables devices that are compact, portable, energy efficient, integrate sensing, and carry out complex functions of a full-scale laboratory
DNA as a BarcodeShort specific DNA sequences for identifying
species (cost of “readers” is decreasing)
Cytochrome C oxidase I
all species have the enzyme with variations from one species to another
May work for birds but not plants – need to identify another gene.
Marshall, Science, 2005
Radio Frequency Identification as a Barcode
RFID (Radio Frequency Identification)
Integrated circuit on a small silicon chip attached to a small, flexible antenna, creating a tag. Integrated circuit provides data storage to record and store information.
Reader sends a signal to the tag.
tag absorbs some of the RF energy from the reader signal and then reflects the RF energy as a return radio signal containing information from its memory.
From Alien website: http://www.alientechnology.com
Microfluidics
Movement of fluids at microscopic level Micron-sized channels and featuresApplications
BiosensorsMicro-bioseparationsPathogen detection
Benefits: Microfluidic Systems-Interrogation of fluid samples
MiniaturizationConsumes less reagentsEnables higher sensitivityShorter analysis time
Rapid Prototyping using PDMS UV
High resolution transparency as maskphotoresistSiO2 or silicone substrate
Develop photoresist obtains master
Pour PDMS over master Cure 70 °C for 1 hour
PDMS
PDMS Release PDMS from master and seal against a flat substrate
Microchannels formed. PDMS
Process takes a day or more
The Need
Simple and rapid fabricationWell defined surface chemistries Flexibility and adaptabilityAmendable for rapid prototyping
techniques
Microfiber Assisted Fabrication in 15 minFlat PDMS cover
Glass fiber (~12 µm diameter)
PDMS
SiO2
SiO2, glass, or PDMS substrate
1 µL labeled avidin (green)
Glass fiber
t = 3 minGlass substrateSiO2 substrate
(a) (b) (c)
Flow channel
1 nL/mm channel
Mixing in Nanoliter Well
Glass fiber (~12 µm dia)PDMS
(a)SiO2 substrate
1 nL well at intersectionLabeled BSA (red, 200 µg/ml)