Fundamentals of Microbiology Biol 315 Lab 2 Instruments and common chemicals in microbiology, Microscopy, Sterilization techniques
Jan 13, 2016
Fundamentals of MicrobiologyBiol 315
Lab 2Instruments and common chemicals in microbiology, Microscopy, Sterilization techniques
Common Instruments
• Autoclave (121◦C, 15 psi)
• Oven (0-300 ◦C)
• Incubator (0-120 ◦C)
• pH Meter
• Hotplate and Magnetic Stirrer
• Laminar Flow Cabinet / Biosafety Cabinet
AUTOCLAVE
http://textbookofbacteriology.net/autoclave1.jpg
http://i01.i.aliimg.com/photo/v0/108072579/Autoclave_Portable_Autoclave_Laboratory_Autoclave_Laboratory_Equipments.jpg
http://www.naanolabindia.com/laboratory-instruments/vertical-autoclave.jpg
http://www.pharmaceutical-int.com/upload/image_files/articles/images/companies/865/large-capacity-autoclave-b.jpg
http://www.theprominex.com/gallery/images/medical/autoclave.jpg
Oven
http://2.imimg.com/data2/FU/BT/MY-2143786/1-250x250.jpg
http://images.hellotrade.com/data/T/W/MY-633236/Hot-Air-Oven---Lab-Model_250x250.jpg
http://faculty.ksu.edu.sa/baddour/PublishingImages/hotairoven.jpg
INCUBATORS
http://www.stuart-equipment.com/adminimages/Incubators_SI505_open.jpg
http://www.stuart-equipment.com/adminimages/Incubators_SI505_closed.jpg
http://www.ika.com/ika/product_art/images/3510000_1.jpg
Shaking Incubator
pH Meter
http://growsetup.files.wordpress.com/2011/02/3505-ph-meter.jpg
Hotplate and magnetic stirrer
http://www.labdepotinc.com/admin/uploads/scilogex-ms-h-s-m-10008-vlarge-r_1.jpg
http://www.troemner.com/images/hotplate_stirrers/adv_hps_lge.jpg
http://pimg.tradeindia.com/00076902/b/0/Laboratories-Magnetic-Stirrer-Hotplate.jpg
Biosafety cabinet
http://www.terrauniversal.com/gallery/hoods/Images/biological_safety_cabinet_purifier_Cover.jpg
Class I Cabinets
• These cabinets have unrecirculated airflow away from the operator that is discharged to the atmosphere after filtration through a HEPA filter.
• They provide good operator protection but do not protect the material within the cabinet (the product) from contamination.
Class II Cabinets
• Class II cabinets are designed for personnel, product and environmental protection.
• They are designed for work involving microorganisms in containment levels 2, 3 and 4 laboratories.
• Class II cabinets are most commonly used in biomedical research laboratories because of their characteristics.
http://escoglobal.com/products/images/products-big/1322810342.jpg
Class III Cabinets
• Class III cabinets are totally enclosed and gas-tight with HEPA filtered supply and exhaust air. Work is performed with attached long-sleeved gloves.
• The cabinet is kept under negative pressure and airflow is maintained by a dedicated exterior exhaust system.
• Class III cabinets protect the worker and the product. They are designed for work with level 4 pathogens.
• The exhaust air is double HEPA filtered or treated by HEPA filter and incineration.
http://www.phac-aspc.gc.ca/publicat/lbg-ldmbl-04/gfx/fig6_e.gif
http://doh.sd.gov/img/bsc.jpg
http://www.nap.edu/books/0309075718/xhtml/images/p20005395g49001.jpg
http://escoglobal.com/products/images/products-big/AC2-D.jpg
http://web.princeton.edu/sites/ehs/biosafety/biosafetypage/BSC2.jpg
Chemicals
• Media (solid and liquid)– Nutrient agar, nutrient broth
– EMB agar, MacConkey’s agar, Blood agar
– Lactose broth
• Gram’s staining kit
• Catalase reagent
• Oxidase reagent
Microscopy
• Light microscope
• Photography and video making through microscope using live camera
• Other kinds of microscopy
Gram’s Staining result
http://janiceatmeredith.files.wordpress.com/2012/01/gramsta.gif
THANK YOU