1 FT-IR ANALYSIS OF DERIDE HUMAN SERUM FOR DETERMINING ALBUMIN CONCENTRATION IN IRAQI PATIENTS WITH RENAL FAILURE *Israa G. Zainal & **Ameer S. Radi *AL-Mustansiriya University ,College Of Science ,Chemistry Department. [email protected], AL-Kademiyah Hospital * * , [email protected]. Abstract This study attempt to evaluate the spectral difference in serum albumin level between healthy and patients with renal failure pre and post dialysis using two methods kit assay & IR spectroscopy . The results showed a non significant difference between the level of albumin measured by kit assay & IR spectroscopy to all studied groups . Correlation studies indicated that there were a positive significant correlation between albumin assayed by kit assay & IR spectroscopy [(r = 0.59 , p = 0.004 ), (r = 0.638 , p = 0.001) and ( r = 0.478 , p = 0.024 )] in control and pre ,post dialysis patients respectively . Keywords: Chronic renal failure, Serum albumin, FT-IR spectroscopy Introduction The kidney plays a central role in the regulation of plasma concentrations of low molecular weight proteins and human serum albumin (1) .Renal failure occurs where there is damage in the kidneys that impairs their ability to filter and remove waste products from the blood. Diagnosis of renal failure is made by collection of blood and urine samples for analysis (2) . Proteins or albumin in serum is commonly tested to monitor liver and renal conditions .Not surprisingly , therefore , serum and blood analysis become one of the most common tests performed and all of the major dissolved metabolites are routinely in clinical laboratory.Most of these analyses are carried out with a photometric methods using an enzymatic reactions .The photometric measurement method is well established and easily available. However , the chemical process such as enzymatic reaction requires expensive reagents and a relatively large volume of the sample. More than 50 of the operating cost of clinical analyzers in the hospital is devoted to the expenses of reagents. Recently , there have been some reports on the optical spectroscopic method for the quantification of major metabolites in human blood , serum , tissue and other substrates (3- 6) .Infrared spectroscopy offers an approach to clinical analysis that is conceptually very appealing . Whereas countless assays rely on the use of chemical agents to " recognize " the analyte of interest and to react with the analyze to produce specific color changes .IR-based analysis is found on the rich IR absorption patterns that characterize the analytes themselves. These absorption patterns provide the basis to distinguish among the constituents and to separately quantify them. The most obvious distinguishing feature is that no reagents are required. In addition , IR-based analytical methods require very small sample volumes (typically micro liters) , show good precision over the entire physiological range , and are well suited for automation (7- 9) . The aim of the present study is to find the spectral difference in serum albumin level between healthy and renal failure (pre and post dialysis) blood sera using kit assay & FTIR spectroscopic technique . Materials and methods Blood samples were collected from (22) patients (12 males and 10 females) , age range between 20 to 70 years with chronic 1
6
Embed
FT-IR ANALYSIS OF DERIDE HUMAN SERUM FOR · PDF fileFT-IR ANALYSIS OF DERIDE HUMAN SERUM FOR DETERMINING ALBUMIN CONCENTRATION IN IRAQI ... spectrophotometer . ... Serum albumin levels
This document is posted to help you gain knowledge. Please leave a comment to let me know what you think about it! Share it to your friends and learn new things together.
Transcript
1
FT-IR ANALYSIS OF DERIDE HUMAN SERUM FOR DETERMINING
ALBUMIN CONCENTRATION IN IRAQI PATIENTS WITH RENAL
FAILURE *Israa G. Zainal & **Ameer S. Radi
*AL-Mustansiriya University ,College Of Science ,Chemistry Department.
This study attempt to evaluate the spectral difference in serum albumin level between healthy and patients with renal failure pre and post dialysis using two methods kit assay & IR spectroscopy . The results showed a non significant
difference between the level of albumin measured by kit assay & IR spectroscopy to all studied groups . Correlation
studies indicated that there were a positive significant correlation between albumin assayed by kit assay & IR
spectroscopy [(r = 0.59 , p = 0.004 ), (r = 0.638 , p = 0.001) and ( r = 0.478 , p = 0.024 )] in control and pre ,post