Freshwater metagenomics NO 3 - N2N2 NH 4 + O2O2 H2H2 Me ox Me red C2H3O2-C2H3O2- CO 2 Acyl-HSL HPO 4 2- C 6 H 12 O 6 SO 4 2- HS - Me 2+ MeS.

Freshwater metagenomics

NO3-

N2

NH4+

O2

H2

Meox

Mered

C2H3O2-

CO2

Acyl-HSL

HPO42-

C6H12O6

SO42-

HS-

Me2+

MeS

Freshwater metagenomics

NO3-

N2

NH4+

O2

H2

Meox

Mered

C2H3O2-

CO2

Acyl-HSL

HPO42-

C6H12O6

SO42-

HS-

Me2+

MeS

Trout Bog0.01 km2, zmax = 8 m

Sparkling Lake0.6 km2, zmax = 20 m

Lake Mendota40 km2, zmax = 24 m

Erken Mälaren

Metagenomic sequencing of Freshwater Microflora in Swedish Lakes

VätternMälaren: Eutrophic, 1140 km2, third largest lake in Sweden, max depth 64m

Erken: Mesotrophic, 24 km2, GLEON site

Vättern: Oligotrophic, 1912 km2, deep (128 m) small drainage area.

Collaborators

• Ramunas Stepanauskas– Bigelow Marine Laboratory, Maine

• Trina McMahon– UW-Madison, WI

• Stefan Bertilsson– Uppsala University, Sweden

Other random stuff…

MERIS satellite for remote sensing

Colleen Mouw, Space Science and Engineering Center, UW

Actual data….