FORENSIC CHEMISTRY AS SCIENTIFIC AND EDUCATIONAL SPECIALISATION AT THE JAGIELLONIAN UNIVERSITY

FORENSIC CHEMISTRY FORENSIC CHEMISTRY AS SCIENTIFIC AND EDUCATIONAL AS SCIENTIFIC AND EDUCATIONAL

SPECIALISATION SPECIALISATION AT THE JAGIELLONIAN UNIVERSITYAT THE JAGIELLONIAN UNIVERSITY

Paweł Kościelniak

JAGIELLONIAN UNIVERSITY, Faculty of Chemistry

INSTITUTE OF FORENSIC RESEARCHCracow, Poland

Jagiellonian University (1364)

Faculty of Law and Administration

Faculty of Philosophy

Faculty of History

Faculty of Philology

Fac. of Math., Phys. and Comp. Sciences

Faculty of Biology and Earth Sciences

Faculty of Biotechnology

Faculty of Chemistry

Fac. of Management and Social Comm.

Fac. of Internat. and Political Studies

Collegium Medicum

Fac. of Medicine & Div. of Dentistry

Fac. of Pharmacy & Div. of Medical Anal.

Faculty of Health Care

Jagiellonian University

Students

Total: 44 214

Regular studies: 27 653

Extramural studies: 15 552

External studies: 1 099

Postgraduate studies: 2 091

Academic staff

total: 6 710

academic teachers: 3 605

professors: 486

doctores habilitati: 503

JU in numbers (2006)

Faculty of Chemistry

Karol Olszewski (1846-1915) Chemist, physicist and expert of low Chemist, physicist and expert of low

temperatures; the first scholar who liquefied temperatures; the first scholar who liquefied nitrogen and oxygen; he also discovered a nitrogen and oxygen; he also discovered a

method of hydrogen liquefaction and method of hydrogen liquefaction and constructed machinery for that purpose, constructed machinery for that purpose,

which enabled him to reach the then-lowest which enabled him to reach the then-lowest world temperature, -225 C. world temperature, -225 C.

191950-5250-52

Faculty of Chemistry

Department of Inorganic Chemistry

Department of Organic Chemistry

Department of Analytical Chemistry

Department of Physical Chemistry

Department of Chemical Physics

Department of Theoretical Chemistry

Department of Crystal Chemistry

Department of Chemical Technology

Department of Chemical Education

Department of Computational Methods in Chemistry

DEPARTMENT OFANALYTICAL CHEMISTRY

(25)

LABORATORY FOR

FORENSIC CHEMISTRY(11)

GROUP OF ANALYTICAL FLOW TECHNIQUES

(10)

GROUP OF CHEMOMETRICS

(4)

Pure analytical chemistry & Flow Analysis

Optimization of analytical conditionsDevelopment of calibration methods Examination of interference effects

Analytical techniques

Atomic absorption spectrometryAtomic fluorescence spectrometry

UV/VIS spectrophotometryElectrochemical techniques

Capillary electrophoresisLiquid chromatography

Forensic Chemistry

Development of analytical methods and procedures for forensic purposes

Chemistry

undergraduate study graduate study

Organic

synthesisCatalysis Forensic

chemistry

EDUCATIONAL STRUCTURE

19291929

It is the only research centre of its kind in Poland, working under the aegis of the Ministry of Justice. Its statutory duties include: - research in the field of forensic science, - preparation of expert opinions for courts and public prosecutor's offices in criminal and civil cases,- the development and promotion of the forensic sciences.

The Institute carries out expert investigations and research in the following units:

Department of Forensic Toxicology Department of Criminalistics Department of Road Accidents Department of Forensic Psychology Forensic Genetics Section

Institute of Forensic Research

before 1997 – informal collaboration between FCh JU and IFR 1997 – common idea of educational module called „forensic chemistry” as one of

objectives of the TEMPUS project „Transformation of University Chemistry Studies at the Jagiellonian University”(TRUCS),

1997/98 – formal introduction of this module to the chemical study at FCh JU 2000 – establishment of the Laboratory for Forensic Chemistry at FCh JU as one

of the objectives of the TEMPUS project „National Training Program for Forensic Science Experts” (NATFOSE),

2001 – formal agreement between FCh JU and IFR in the field of forensic

chemistry

2000/2001 – modification of the curriculum of module „forensic chemistry”

from 2002 – teaching of „forensic chemistry” according to new curriculum

MILESTONES

LABORATORY FOR FORENSIC CHEMISTRY

- system for capillary electrophoresis (PrinCe 550 CSD, Prince)

- HPLC system (LaChrom, Merck)

- atomic fluorescence spectrometer with the flow system for hydride generation (AFS 230, Beijing Haiguang Instrument

Co.)

- system for microwave digestion (MARS 5X, CEM), - audiovisual equipment (video-projectors, digital photo-cameras, video-camera, video-recorders)

founded in 2000

Sem. SubjectNo. of hours

lectures classes seminars total

VIElectronics and automatics 30 30Optional 60 60

Total 90 90

VII

Electronics and automatics 60 60

Advanced analytical chemistry 45 45

Chemometrics and biometrics 30 15 45

Analysis in forensic chemistry 15 15

Radiometric analysis 30 30

Toxicology 30 30

Flow analysis 15 15

Optional 60 60

Total 225 60 15 300

Sem. SubjectNo. of hours

lectures classes seminars total

VIII

Radiometric analysis   45   45Forensic toxicology   60 15 75Criminalistics 15 60 15 90Low aspects of the forensic expert work 15   15

Student’s report   30 30Optional 60   60

Total 100 100 100 315

IXiX

Laboratory for master thesis   450 450

Seminar for master thesis     60 60Clinical toxicology 15   15GLP 15   15

Optional 60   60Total 90 450 60 600

No. Place

1 Toxicological analysis of drugs IFR2 Ethanol- selected toxicological and analytical aspects IFR3 Application of the GC/MS method to analysis of medicines in

biological materialsIFR

4 Determination of inorganic toxicants in biological material IFR5 Chemical examinations of car paints IFR6 Chemical examinations of fibers IFR7 Chemical examinations of gun-shot residuals IFR8 Chemical examination of inks by TLC IFR9 Chemical analysis of inks by CE JU

10 Determination of Se and As in biological materials by AFS-HG JU11 Comparative examination of psychotropic medicines by HPLC

and CEJU

12 Profiling of drugs – physicochemical and chemometric examination

JU

LABORATORY CLASSES

SubjectAnalytical method

FCh JU IFR

Examination of questioned documents on the basis of identification of chemical composition of inks CE

FTIR, TLC, RAMAN

Development of analytical procedures for determination of psychotropic drugs, particularly of phenotiazines and tricyclic antidepressants

HPLC, CE GC/MS

Development of analytical procedures for determination of selenium and arsenic in biological materials (blood, urine, hair, body tissues)

AFS, AAS UV/VIS

Identification and discrimination of car traces (paint, oil) - physicochemical and chemometric examinations

ASA FTIR, XRF

Profiling of amphetamine - physicochemical and chemometric examinations

TLC GC/MS

RESERCH SUBJECTS

PUBLICATIONS

- J. Zięba-Palus, P. Kościelniak, An Analysis of the Similarity of Motor Oils on the Basis of Their Elemental Composition, Forensic Sci. Int., 112, 81-90 (2000).

- J. Zięba-Palus, P. Kościelniak, M. Łącki, Differentiation of Motor Oils on the Basis of Their IR Spectra with Application of the Cluster Analysis, J. Molec. Struct., 596, 221-228 (2001).

- J. Zięba-Palus, P. Kościelniak, M. Łącki, Differentiation of Motor Used Oils on the Basis of Their IR Spectra with Application of the Correlation Method, Forensic Sci. Int., 122, 35-42 (2001).

- K. Madej, Capillary Electrophoresis as a New Tool in Forensic Toxicological Analysis, Problems of Forensic Sciences, 48, 31-52 (2001).

- R. Wietecha, R. Stanaszek, Determination of Morfine, Codeine and 6-monoacetylomorphine in Hair of Addicted Persons, Problems of Forensic Sciences, 49, 38-45 (2002).

- J. Mania, K. Madej, P. Kościelniak, Inks Analysis by Capillary Electrophoresis – Analytical Conditions Optimisation, Chem. Anal., 47, 585-594 (2002).

- P. Kościelniak, W. Piekoszewski, (ed.) Forensic Chemistry (in Polish), IFR, Kraków 2002.

- Z. Chłobowska, E. Chudzikiewicz, P. Kościelniak, W. Piekoszewski, A. Stolarz, Application of Gas Chromatography with NPD Detection to the Analysis of Tricyclic Antidepressants in Blood, Chem. Anal. 48, 255-263 (2003).

PUBLICATIONS

- J. Zięba-Palus, P. Kościelniak, Differentation Between Used Motor Oils on the Basis of Their IR Spectra for Criminalistic Purposes, The Forensic Scientist, 1, 23-28 (2003).

- R. Wietecha, P. Kościelniak, T. Lech, M. Rymanowski, Determination of Selenium in Human Blood Using Atomic Fluorescence Spectrometry, Problems of Forensic Sciences, 52, 21-30 (2003).

- Z. Chłobowska, Cz. Świegoda, P. Kościelniak, W. Piekoszewski, Identification and Determination of Flunitrazepam and Its Metabolites in Blood by Gas Chromatography, Chem. Anal., 49, 71-77 (2004).

- R. Wietecha, P. Kościelniak, T. Lech, T. Kielar, Simple Method for Simultaneous Determination of Selenium and Arsenic in Human Hair by Means of Atomic Fluorescence Spectrometry with Hydride Generation Technique, Microchim. Acta, 149, 137-144 (2005).

- K. Madej, M. Woźniakiewicz, M. Kała, Method for Screening and Quantification of Seven Phenothiazines in Whole Blood Samples by Non-aqueous Capillary Electrophoresis, Chromatographia, 61, 259-263 (2005).

- K. Madej, M. Kała, M Woźniakiewicz Application of Liquid Chromatography and Non-aqueous Capillary Electrophoresis to the Determination of Phenothiazines in Six Cases of Poisonings: Comparative Analysis, Chromatographia, 62, 533-538 (2005).

- R. Wietecha, J. Dobrowolska, P. Kościelniak, Simple Analytical Method for Determination of Selenium and Arsenic in Human Urine, 39, 2787-2796 (2006).

PROJECTS

TEMPUS program, project no. 12236/97 „Transformation of University Chemistry Studies at the Jagiellonian University” (TRUCS); 1997-2000

TEMPUS program, project no. 13514/98 „National Training Program for Forensic Science Experts” (NATFOSE); 1998-2001

LEONARDO DA VINCI program, project PL/01/A/Exd/14054 „Vocational Training System in Forensic Sciences - Exchange of Educational Experiences”

(VITSFOS-E); 2001-2003

NATO program, project no. PST.CLG.980067 “Sensors for Chemical Weapons Detection Based on Molecularly Imprinted Polymers”; 2004-2005

TEMPUS program, project no. 41105 - 2006„Education System In Forensic Sciences for the Republic of Macedonia” ”

(EDUFORMAK); 2007-2009

Ph.D. STUDYStudent Subject Defense

Renata WietechaDevelopment of the analytical procedure for the selenium determination in human tissues by the AFS-HG technique

2004

Rafał Borusiewicz Examination of arson traces 2004

Joanna Mania Examination of inks by capillary electrophoresis 2005

Małgorzata Świst Profiling of drugs 2005

Maria Pawluk-Kołc Classification and discrimination of glass traces 2005

Agnieszka Skulska Profiling of drugs 2006

Agnieszka Zakrzewska Profiling of drugs 2006

Maciej Kochanowski Analytical examination of illicit drugs 2007

Michał Woźniakiewicz Analysis of psychotropic drugs by HPLC and CE 2008

Justyna Dobrowolska Elemental analysis of biological materials 2008

Jakub Mielczarek Classification and discrimination of car paint traces 2008

FUTURE

• to make specialisation in forensic chemistry more and more attractive (e.g. to organise „artificial” trials, to prepare new interactive classes with the use of audiovisual and computer tools),

• to organise and open post-graduate study in the field of

forensic chemistry,

• to organise and open the „forensic” courses in the frame of „virtual university”

• to increase the scientific level of the forensic research performed,

• to expand the collaboration with national and foreign forensic/analytical centres

„FORENSIC CHEMISTRY” IN NUMBERS

• first and still only one activity of this kind in Poland

• 2 years of studying (4th and 5yh year of chemical study)

• 10 years of activity (since 1997/78)

• 12 - 14 students a year

• 31 publications (with the student contribution)

• 34 master’s theses

• 82 graduates

• a lot of students interested in the subject every year