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Food Safety / Analytic Kits (Price List 2014 • Distributors)
Immunolab
Page 1 of 2
Immunolab GmbH fon +49 (0)561 491 742-0 mail [email protected] Otto-Hahn-Straße 16, 34123 Kassel, Germany fax +49 (0)561 491 742-20 web www.immunolab.de
PREL-FA_dis_eng_v3
Food ELISA (96 Tests)
Cat. No. Product Name Price (EUR) 1
Mycotoxins
AB1-E01 Aflatoxin B1 195
AM1-E01 Aflatoxin M1 250
DON-E01 Deoxynivalenol (DON) 230
DON-E03 Deoxynivalenol (DON) RAPID 230
FUM-E01 Fumonisin 230
FUM-E03 Fumonisin RAPID 230
OTA-E01 * Ochratoxin A 230
ZEA-E01 Zearalenone 230
ZEA-E03 Zearalenone RAPID 230
T2T-E01 T2-Toxin 230
Vitamins
FOL-E01 Folic Acid 270
B12-E01 Vitamin B12 270
BIO-E01 Vitamin H (Biotin) 270
Antibiotics
CAP-E02 Chloramphenicol 230
PEN-E01 Penicillin 250
STR-E02 Streptomycin 250
TCY-E01 * Tetracycline upon request
Hormones
EST-E01 Estradiol 195
PRO-E01 Progesterone 195
TES-E01 Testosterone 195
Miscellaneous
HIS-E02 Histamine 270
HIS-E03 Histamine RAPID 270
* in development
Food Safety / Analytic Kits (Price List 2014 • Distributors)
Immunolab
Page 2 of 2
Immunolab GmbH fon +49 (0)561 491 742-0 mail [email protected] Otto-Hahn-Straße 16, 34123 Kassel, Germany fax +49 (0)561 491 742-20 web www.immunolab.de
PREL-FA_dis_eng_v3
Food ELISA (96 Tests) continuation
Cat. No. Product Name Price (EUR) 1
Allergens
ALM-E01 Almond 270
BLG-E01 Beta-Lactoglobulin 270
CAS-E01 Casein 270
CAW-E01 Cashew 270
CEL-E01 * Celery 270
CRU-E01 Crustaceans (Tropomyosin) 270
EGG-E01 Egg White 270
FIS-E01 Fish (Parvalbumin) 270
GLU-E02 Gluten / Gliadin 270
HAZ-E01 Hazelnut 270
LUP-E01 Lupine 270
LYS-E01 Lysozyme 270
MIL-E01 Milk 270
MUS-E01 Mustard 270
OVA-E01 Ovalbumin 270
PEA-E01 Peanut 270
PEC-E01* Pecan Nut 270
PIS-E01 Pistachio 270
SES-E01 Sesame 270
SOJ-E01 Soja (Soy) 270
WAL-E01 Walnut 270
* in development
Food Immunoaffinity Columns (10 columns / kit)
Cat. No. Product Name Price (EUR) 1
AFB-A01 Aflatoxin (total) 65
AB1-A01 Aflatoxin B1 65
B12-A01 Vitamin B12 95
BIO-A01 Vitamin H (Biotin) 95
ZEA-A01 Zearalenone 80
1 All prices are given in EURO, net ex works, exclusive of packaging.
Food Intolerance ELISA Tests (Price List 2014 • Distributors)
Immunolab
Immunolab GmbH fon +49 (0)561 491 742-0 mail [email protected] Otto-Hahn-Straße 16, 34123 Kassel, Germany fax +49 (0)561 491 742-20 web www.immunolab.de
PREL-FI_dis_eng_v2
Cat. No. Product Name Price (EUR) 1
Food IgG ELISA 2
ILE-SCG25 Specific IgG Screening, Nutritional, 3x 24 food antigens 80
ILE-SCG26 Specific IgG MultiScreen, Nutritional, 1x 88 food antigens 80
Food IgG4 ELISA 2
ILE-SCG06 Specific IgG4 Screening, Nutritional, 4x 20 food antigens 80
ILE-SCG80 Specific IgG4 MultiScreen, Nutritional, 1x 88 food antigens 80
ILE-SCG210 Specific IgG4 Screening, Nutritional, 3x 24 food antigens 80
Coeliacal Antibodies
ILE-GLI01 Gliadin / Gluten IgG, 96 tests 90
ILE-GLI02 Gliadin / Gluten IgA, 96 tests 100
ILE-GLI03 Gliadin / Gluten IgM, 96 tests 100
1 All prices are given in EURO, net ex works, exclusive of packaging.
2 For Food IgG and IgG4 various formats (number of food antigens per microtiter plate) and
invidual, regional specific food antigen compositions can be ordered.
* All prices are given in EURO, net ex works, exclusive of packaging.
Food Safety / Analytic Kits
Newsletter Allergen ELISA Kits Immunolab
Immunolab GmbH fon +49 (0)561 491 742‐0 mail [email protected] Otto‐Hahn‐Straße 16, 34123 Kassel, Germany fax +49 (0)561 491 742‐20 web www.immunolab.de
PRIN-FA_allergens_eng_v5
Food Allergy is a rapidly increasing area of consumer concern. Food allergens are proteins in food that, even in low concentrations, can trigger allergic, sometimes life‐threatening reactions in sensitive individuals.
As protection of the consumer, the EU directive 2003/89/EC was issued in November 2005. It obliges the food manufacturers to label their products with all ingredients capable of causing an allergic reaction. Annex IIIa includes a list of food ingredients classified as being possible allergens or intolerants, e.g. peanuts, hazelnuts, eggs, gluten, almond, milk, soy, crustaceans and celery.
Testing for the presence of food residues provides security for food manufacturers that a potentially dangerous ingredient is not included in their food products.
Immunolab GmbH as an acknowledged developer and manufacturer of high‐quality Food Safety/Analytic and Clinical Diagnostic products, hereby introduces its new food allergen product line to detect almond, beta‐lactoglobulin, casein, crustaceans, egg white, fish, gluten, hazelnut, lupine, mustard, peanut, soy and walnut residues in raw materials and processed foods. Further parameters (Celery, Fish) are in development. The Immunolab food allergen kits are 96 well ELISA tests and can be processed according to a usual standard sandwich ELISA protocol. Each kit includes all components required to perform the assay. Features and Benefits • Easy Handling by:
o Standardized Test Protocol o Short Assay Time, 60 minutes (20/20/20 min) o Reagents (excl. wash buffer) ready‐to‐use; breakable strips o Simple extraction procedure and no further clean‐up steps o Incubation at ambient temperature
• High Accuracy and Reproducibility • High Stability, minimum shelf life 12 months
Technical Performance Data
Analyte LOD LOQ Ref. Range Recovery Precision (intra‐assay)
Food Intolerance Tests » For a Better Quality of Life «
Relevance of the detection of specific IgG / IgG4 Immunolab antibodies in the diagnosis of food intolerances
Page 1
Immunolab GmbH fon +49 (0)561 491 742‐0 mail [email protected] Otto‐Hahn‐Straße 16, 34123 Kassel, Germany fax +49 (0)561 491 742‐20 web www.immunolab.de
PRIN-FI_background_eng_v1
There is increased clear epidemiological evidence during recent years that more and more people suffer from food intolerance symptoms. An analogous tendency is also observed for classical allergic diseases (IgE mediated, type 1, immediate reaction). The use of IgG and/or IgG4 tests to detect the triggering food antigens is still a controversially discussed subject among medical experts. Despite this situation the detection of IgG/IgG4 antibodies should always be considered if typical causes can be excluded like lactose or fructose malabsorption, celiac disease or an IgE mediated food allergy (type 1).
The allergic immediate reaction (type 1) is associated with a Th2 cell activation. Resulting from both the allergen portal of entry and exposure time the Th2 activation can switch to a more Th1 directed reaction leading to the formation of IgG4 and IgA antibodies. If this effect increases further towards Th1, IgG1 antibodies can be produced.
Specific IgG antibodies can be found in the serum of food allergy patients as well. In fact, numerous studies have described higher IgG4 levels in patients with IgE mediated symptoms (type 1) compared to non allergic individuals. The extent of the allergen exposure and exposure time have a strong impact on the concentration of IgG4 antibodies. This leads to the conclusion that an IgG4 reaction cannot be regarded as a physiological reaction only but has a pathological significance from a certain reactivity level.
There are four IgG subclasses of which IgG1 and IgG4 play a particular role in food intolerances. IgG1 is produced as initial response after contact with a new food antigen. After further exposure to the antigen a switch occurs towards formation of IgG4 antibodies
(1).
The IgG4 portion of total IgG can increase from 3‐5% to 50%. The elevated IgG4 concentration points to an intense conflict between the immune system and the triggering food antigen. Functionally, clear evidence could be obtained that IgG4 antibodies are able to sensitize cells temporarily but can block the allergic response as well. In fact 50% of untreated allergic patients show elevated IgG4 levels. Often disorders appear after hours only, causing difficulties to identify the triggering food. In many cases a disturbed intestinal permeability is postulated to initiate the food intolerance. This leads to a permanent exposure of the immune system by antigenic structures. The overload of the immune system can result in an enhanced sensitivity to infections.
Regarding the diagnostic relevance of IgE‐ and IgG/IgG4 reactions it should be considered that immunological mechanisms and related symptoms can be different in inhalation allergies and food intolerances. In particular a non‐IgE mediated reaction is known for food intolerances for a long time.
Food Intolerance Tests » For a Better Quality of Life «
Relevance of the detection of specific IgG / IgG4 Immunolab antibodies in the diagnosis of food intolerances
Page 2
Immunolab GmbH fon +49 (0)561 491 742‐0 mail [email protected] Otto‐Hahn‐Straße 16, 34123 Kassel, Germany fax +49 (0)561 491 742‐20 web www.immunolab.de
PRIN-FI_background_eng_v1
The critics of IgG/IgG4 tests, mainly allergologists and their professional associations, persistently complain about the lack of scientific studies, without presenting concrete own data though. Indeed numerous articles have been published during recent years which support the relevance of the IgG/IgG4 antibodies’ detection for the diagnosis of food intolerances. The conclusion of some of these publications are presented as follows:
A Norwegian group postulated an important immunopathological role of specific IgG4 antibodies in patients with a delayed non‐IgE mediated cow’s milk allergy. This patient group showed significantly higher concentrations of ß‐lactoglobulin (ß‐LG)‐ specific IgG4 antibodies compared to cow’s milk tolerized patients. (2)
A food intolerance reflected by IgG antibodies can be associated with an increased risk of arteriosclerosis and low grade inflammation (elevated CRP levels) in obese juveniles. (3)
Other publications support the high significance of IgG4 tests to determine and monitor an elimination diet in patients with an irritable bowel syndrome. Moreover, the investigations of Zar point to a positive impact on psychic symptoms, based on IgG4 tests.
(4‐7)
Bernardi has found highly significant differences of IgG4 titers in patients with food intolerance to 19 food antigens compared to asymptomatic healthy subjects. The obtained sensitivity and specificity were 81% and 87%, respectively. Based on the described test results exclusion diets were performed leading to a significant improvement of the symptoms in 78.5% of all cases after 2 months only. An IgG4 control testing after this time period proved a manifest reduction of the previously elevated IgG4 titers.
(8)
Even in migraine patients symptoms could be improved by exclusion diets that were based on IgG results. (9)
A less recent publication describes the detection of high IgG4 concentrations in patients with confirmed sensitization to soy beans. In contrast low reactivity was found for IgE and IgG. (10)
In another investigation of patients showing asthma symptoms after contact with soy dust authors found that both specific IgE and IgG4 antibody levels to soy were increased. They postulated that the IgG4 increase is part of the pathological asthma process. (11)
Food Intolerance Tests » For a Better Quality of Life «
Relevance of the detection of specific IgG / IgG4 Immunolab antibodies in the diagnosis of food intolerances
Page 3
Immunolab GmbH fon +49 (0)561 491 742‐0 mail [email protected] Otto‐Hahn‐Straße 16, 34123 Kassel, Germany fax +49 (0)561 491 742‐20 web www.immunolab.de
PRIN-FI_background_eng_v1
References
1. Snapper et al. (1999) in Fundamental Immunology, ed. Paul, W. E. (Lippincott, Philadelphia), pp. 831–861.
2. Sletten et al. (2006). Changes in humoral responses to beta‐lactoglobulin in tolerant patients suggest a particular role for IgG4 in delayed, non‐IgE‐mediated cow's milk allergy. Pediatr. Allergy Immunol. 17, 435‐443.
3. Wilders‐Truschnig, M. et al. (2008). IgG Antibodies against Food Antigens are Correlated with Inflammation and Intima Media Thickness in Obese Juveniles. Exp Clin Endocrinol Diabetes 116(4):241‐5.
4. Atkinson et al. (2004). Food elimination based on IgG antibodies in irritable bowel syndrome, a randomised controlled trial. Gut 53:1459–1464.
5. Zar et al. (2005). Food‐specific IgG4 antibody‐guided exclusion diet improves symptoms and rectal compliance in irritable bowel syndrome. Scand J. Gastroenetrol. 40, 800‐807.
6. Zar et al. (2005). Food‐Specific Serum IgG4 and IgE Titers to Common Food Antigens in Irritable Bowel Syndrome. Amer. J. Gastroenetrol. 100, 1550‐1557.
7. Drisko et al. (2006). Treating irritable bowel syndrome with a food elimination diet followed by food challenge and probiotics. J. Amer. College Nutrition 25(6), 514‐522.
8. Bernardi et al. (2008). Time to reconsider the clinical value of immunoglobulin G4 to foods. Clin. Chem. Lab. Med. 46, 687‐690.
9. Hernandez et. al. (2007). Food allergy mediated by IgG antibodies associated with migraine in adults. Rev Alerg Mex 54(5), 162‐168.
10. Awazuhara et al. (1997). Major allergens in sojabean and clinical significance of IgG4 antibodies investigated by IgG and IgG4 immunoblotting with sera from sojabean sensitive patients.Clin. Exp. Allergy 27, 325‐332.
11. Codina et al. (1997). Specific immunoglobulins to soybean hull allergens in soybean asthma, Chest 111, 75‐80.
Infectious Disease ELISA » We Protect Your Health «
Immunolab
Immunolab GmbH, an acknowledged developer and manufacturer of high‐quality immunodiagnostic and analytical reagents, provides you with a wide range of enzyme immunoassays for the detection of infectious disease parameters. Our product line is suitable for routine applications as well as research purposes.
The Immunolab Infectious Disease Tests are microtiter plate based enzyme immunoassays (ELISA) for the qualitatitive and quantitative determination of IgA, IgG and IgM antibodies to multiple infectious agents in human sera and plasma. Further applications in other body fluids are possible.
Each kit includes all ingredients required to perform the assay. One kit provides 96 tests. All reagents are ready‐to‐use. Assay Performance Scheme:
Infectious Disease ELISA – TORCH » We Protect Your Health « Immunolab
Immunolab GmbH fon +49 (0)561 491 742‐0 mail [email protected] Otto‐Hahn‐Straße 16, 34123 Kassel, Germany fax +49 (0)561 491 742‐20 web www.immunolab.de
PRIN-IN_torch_eng_v1
TORCH is an acronym for a group of infectious diseases showing an increased prevalence to cause illness in pregnant women and birth defects in their newborns. The TORCH panel consists of the following tests:
Toxoplasma gondii, Rubella virus, Cytomegalovirus (CMV) and Herpes simplex virus type 1 + 2.
Immunolab GmbH, an acknowledged developer and manufacturer of high‐quality immunodiagnostic and analytical reagents provides you with the complete TORCH panel. Our highly sensitive and specific Toxoplasma, Rubella, Cytomegaly and Herpes simplex kits are in compliance with the European in‐vitro diagnostic directive (IVDD) 98/79/EC and as such are CE marked. The Immunolab TORCH tests are microtiter plate based enzyme immunoassays (ELISA) for the qualitatitive and quantitative determination of IgG, IgM and IgA (only HSV) antibodies to Toxoplasma gondii, Rubella, Cytomegalovirus (CMV) and Herpes simplex virus in human sera and plasma.
Each kit includes all components required to perform the assay. One kit provides 96 tests. All reagents are ready‐to‐use. Assay Performance Scheme:
Features and Benefits
• Short Assay Time
• Easy Handling by:
o Uniform Test Format
o Ready‐to‐use and interchangeable Reagents
o Breakable Strips
o Standardized Performance (identical volumes, incubation times, dilutions)
o Incubation at ambient temperature
• High Stability, minimum shelf life 12 months
• Test results are qualitatitive (using cut‐off) and/or quantitative (using calibrators)
• Quantitative Result with just one sample dilution
• Reference curve calibrated against International WHO Reference Preparations: o Rubella IgG, 1st Intl. Standard RUBI‐1‐94. o Toxoplasma IgG, standard TOXM (3rd Intl. Standard)
• Product Registration acc. to the Europ. in vitro diagnostic directive IVDD 98/79/EC