Folded and unfolded Proteins in their native state, tend to produce multiply charged ions covering a smaller range of charge states. These charge states tend to have fewer charges than an unfolded protein would have, due to the inaccessibility of many of the protonation sites. Increasing the sampling cone voltage may provide sufficient energy for the protein to begin to unfold and create a wider charge state distribution centering on more highly charged ions in the lower m/z region of the spectrum.
Folded and unfolded. Proteins in their native state , tend to produce multiply charged ions covering a smaller range of charge states. These charge states tend to have fewer charges than an unfolded protein would have, due to the inaccessibility of many of the protonation sites. - PowerPoint PPT Presentation
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Folded and unfolded
Proteins in their native state, tend to produce multiply charged ions covering a smaller range of charge states. These charge states tend to have fewer charges than an unfolded protein would have, due to the inaccessibility of
many of the protonation sites.
Increasing the sampling cone voltage may provide sufficient energy for the protein to begin to unfold and create
a wider charge state distribution centering on more highly charged ions in the lower m/z region of the spectrum.
Folded and unfolded statesHolo and apo proteins
30-Oct-199814:50:44apo-pseudoazurin + Cu BMB, The University of Leeds