FLUORESCENCE FLOW CYTOMETRY The Sysmex and haematology analyzers incorporate the ingenious Fluorescence Flow Cytometry technology, which was successfully introduced with top-of-the-end Sysmex . These X-series analyzers are equipped with excellent capabilities offering optimal differentiation between normal cells and pathological cells. XT-series XS-series XE-2100 range Q. How does Fluorescence Flow Cytometry function in X-series analyzers? A. As depicted in the image of Optical System of X-series analyzers, it detects each cell from three angles. 1. Forward Scattered Light (Information on Cell size) 2. Side Scattered Light (Information on Internal Cell structure) 3 . Side Fluorescence Light (Information on RNA/DNA content) Q. What is the role of Side or Lateral Fluorescence Light? A.Fluorescence Light detects DNA/RNA Information of cells along with information about cell activities such as Duplication activity of the nucleus (High RNA) Cytoplasm activity (Protein synthesis etc) This information is useful for very good separation of mature and immature cells. Q. What are the benefits of Fluorescence Flow Cytometry? Q. What is FAD? A. The major benefit of Fluorescence Flow Cytometry to its users is improved detection of pathological cells like Immature Granulocyte Count, High Fluorescence Lymphocyte Count, Abnormal Lymphocyte Detection etc. The other advantage is improved quality of 5 part differential of WBC with 48 hours stability after blood collection and that also for high linear ranges (no dilution necessary). It has a stable and long life detector system requiring no calibration. A. FAD is an acronym for Fluorescent Activated Differentiation used by X-series analyzers to do 5 part differentiation of WBC using Fluorescence Flow Cytometry. FREQUENTLY ASKED QUESTIONS Q. What is source of Fluorescence Light? A. Sysmex uses patented Polymethyne dye to stain DNA/RNA of cells. The stained DNA/RNA of the cells scatters 633 nm laser light, which is measured at 90° as Side Fluorescence Light (SFL).