1 Flow Injection Analysis (FIA) Flow Injection Analysis Flow Injection Analysis Chemiluminescence – Trace Metals ( e.g. dFe) Spectrophotometric – Nutrients S T H A W Time [S] Flow Injection Analysis Flow Injection Analysis Typical FI detection response output: S is sample injection; W is peak width; A is peak areas; H is peak height and T is residence time.
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Flow Injection Analysis (FIA)
Flow Injection AnalysisFlow Injection Analysis
Chemiluminescence – Trace Metals ( e.g. dFe)
Spectrophotometric – Nutrients
S
T
HA
W
Time [S]
Flow Injection AnalysisFlow Injection Analysis
Typical FI detection response output: S is sampleinjection; W is peak width; A is peak areas; H ispeak height and T is residence time.
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Co
Sample injection
Flow Injection AnalysisFlow Injection Analysis
Co
Sample injection
C max
Flow Injection AnalysisFlow Injection Analysis
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Flow Injection AnalysisFlow Injection Analysis
Flow Injection AnalysisFlow Injection Analysis
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Flow Injection AnalysisFlow Injection Analysis
Sample
Waste
Waste
Acid
Buffer
ICP-MS
column
Flow Injection AnalysisFlow Injection Analysis
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Flow Injection AnalysisFlow Injection Analysis
Flow Injection AnalysisFlow Injection Analysis
0 50 100 150 200 250 300
-0.002
0.000
0.002
0.004
0.006
0.008
0.010
0.012
0.014
0.016
0.018
0.020
Absorb
ance (
A.U
.)
Time (sec)
Reproducibility
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Samplepump
NO
Sample loop
COMM COMM
Waste
Carrierpump
Ultra-purewater
NC NC NO
NC
COMM
NC
NO NO
Reagentpump
Ammoniummolybdate
Tin(II)chloride
Reagentpump
Coil 1 (30 cm)
Sample
T-piece
T-piece
Coil 2 (30 cm)
Coil 3 (90 cm)
Detector
COMM
Flow Injection AnalysisFlow Injection Analysis
- r2 = 0.998
- LOD = 0.67 µM PO4-P
- Linear Range = 0.8 - 50 µM - Sample throughput = 40 samples h-1
-200 0 200 400 600 800 1000 1200 1400 1600 -0.015
-0.010
-0.005
0.000
0.005
0.010
0.015
0.020
0.025
0.030
0.035
0.040
8.0 uM
6.0 uM
4.0 uM
1.5 uM
0.8 uM
-500 0 500 1000 1500 2000 2500 3000 3500 4000-0.10
-0.08
-0.06
-0.04
-0.02
0.00
0.02
0.04
0.06
0.08
0.10
Abs
orba
nce
(A.U
.)
Time (s)
Flow Injection AnalysisFlow Injection Analysis
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Flow Injection-Capillary Electrophoresis (FI-CE)
-CE: Excellent tool for examining many types of species includingproteins, DNA, sugars, cations and anions and drugs.
“Achilles Heel” – sample introduction
W
Pump
B
S
A
W
C
B
SV1 SV2
+-SC
SFC
-FI: Powerful sample introduction/manipulation tool
FI-CEPotential in clinical and forensic, biological, environmental andpharmaceutical analysis.
Flow Injection Analysis Flow Injection Analysis –– Capillary Electrophoresis Capillary Electrophoresis
Flow Injection AnalysisFlow Injection Analysis
www.ntmuk.edu/ce
Figure provided in part by: Dr. Gomez
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Detector
Separation capillary
200 μm Fiber Optic Cable
200 μm Fiber Optic Cable
Flow Cell
Electrolytereservoir
Plexiglass block
Flow Cell/Detector Set-up
Separation capillary
VoltagePt
Waste
FI Manifold
Hanrahan & Gomez, 2005
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1. What are the key components to Flow Injection (FI)?
2. How does sample movement within the tubing affect the shapeof an FI peak?
3. What are the advantages of combining FI with otherinstrumentation (e.g. FI-CE)?
In Class Questions
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