The genus Cruziohyla Faivovich et al. (2005) has two species: Cruziohyla calcarifer and C. craspedopus. Cruziohyla craspedopus occurs in Colombia, Ecuador, Peru, Brazil, and probably in Bolivia (Frost, 2014). It is known to occur in three states in the Brazilian Amazon: Castanho, Amazonas (Lima et al., 2003), Cotriguaçu, Mato Grosso (Rodrigues et al., 2011), and Humaitá, Rondonia (Meneghelli et al., 2011). Here, we present the first record of this species for the state of Acre, in Tarauacá (-7.978131 and -71.451686, 240 m; Figure 1). The conservation status of Cruziohyla craspedopus is of least concern (Angulo et al., 2004), since this species has a wide distribution, within large protected areas, despite the degradation of land for pastures and crop plantation. Moreover, the geographical distribution of C. craspedopus overlaps protected areas in Ecuador, Peru, and Colombia (Ron, 2001; Ruiz-Carranza et al., 1996). Between 15th and 31th January 2014, during the rainy season, we found an adult male of Cruziohyla craspedopus (SVL 61 mm) calling on a palm leaf, at approximately 4 m height, near a large temporary pond, 50 m away from a stream, at 21:00 (25°C). This individual was collected and killed with 10% lidocaine, fixed in 5% formaline, and conserved in 70% ethanol. This specimen is housed at the Herpetological Collection of Universidade Federal do Acre (UFAC - 6113). The individual collected showed the characteristic coloration of Cruziohyla craspedopus (see Hoogmoed and Cadle 1991), having dark green head, body, forearms, thighs, shanks, and dermal fringes; along with numerous grayish white blotches scattered on dorsal surfaces, varying from thin cobweb-like patterns to large irregular blotches; grayish white iris with fine black reticulations. A narrow, shallowly scalloped dermal fringe is present on the margin of the lower jaw, and on the outer edge of the forearm. The outer edge of the tarsus bears a broad dermal fringe that is expanded distally into a long pointed calcar and two additional triangular flaps (Figure 2) This new record fills the gap in the species range and expands its geographical distribution in 1,380 Km southwesternwards from Castanho, 1,470 Km westwards from Cotriguaçu, 840 km westwards from Herpetology Notes, volume 7: 479-480 (2014) (published online on 25 August 2014) First record of Cruziohyla craspedopus (Anura: Hylidae) for the State of Acre, with an updated distribution map Nathocley Mendes Venancio¹*, André Luis Botelho de Moura², Thaline de Freitas Brito², Tomaz Nascimento de Melo¹ and Moisés Barbosa de Souza³ 1 Programa de Pós graduação em ecologia e manejo dos recursos Naturais, Universidade Federal do Acre – UFAC, Rio Branco – Acre, Brasil. 2 Programa de Pesquisa em Biodiversidade – PPBio, Núcleo Regional Acre, Universidade Federal do Acre - UFAC. 3 Centro de Ciências Biológicas e da Natureza, CCBN, Universidade Federal do Acre – UFAC. * Corresponding author: [email protected] Figure 1. Known distribution of Cruziohyla craspedopus. 1= type locality (Funkhouser, 1957); 2 = Castanho – Amazonas (Lima et al., 2003); 3 = Cotriguaçu – Mato Grosso (Rodrigues et al., 2011); 4 = Humaitá - Rondonia (Meneghelli et al., 2011) and 5 = Tarauacá, Acre (This study).