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Stuot..-Date: Deceh.

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A. Use the two graphs below to answer the following four questions. Each graph plots thereaction rate of Enzyme A (thick line) and Enzyme B (thin line) against either pH ortemperature.

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~~ic~.lwO enzymes operates over a narrower pH range? (l pt)~' 8

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3. Which of the two enzymes shows greater activity when placed in arefrigerator? (l Pi

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Which data set below best fits Enzyme A? (1 pt)4.

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B. In the space provided, draw a graph that represents the substrate concentration data shown inthe table below. Draw a vertical line to indicate that point at which the enzyme issaturated. (1 pt)

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c. You have set up two vials to measure respiration in peas in cold and warmenvironment.s. You placed 15 sprouts into each of two vials also containing a cotton balland KOH. You then placed a graduated 1ml pipette on the end of each vial. You placedone in a warm environment and the other in a cold environment.

. a.. After ~hat direction did the water in the warm environment pipette

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D. You and your lab partners added yeast and one of the test solutions to fill a small testtube. You then inverted a large tube over each small tube and inverted the two tubes.After taking initial measurements, all 6 were then placed in a warm water bath in hopesthat the yeast would respire. You remeasured each tube at 30, 60 and 90 minutes andmade the appropriate calculations to complete the data table below. Use this data table toanswer the questions that follow.

1. In which solution(s) did the yeast respire most over the entire course of the experiment?

(1 pt) --------' ~\\,\to~~

2. In which solution(s) did the yeast respire most over anyone 30 minute time period? (1 pt)

~E. You and your lab partners added yeast and one of the combinations oftest solutions to fill

a small test tube. You then inverted a large tube over each small tube and inverted the twotubes. After taking initial measurements, all 5 were then placed in a warm water bath inhopes that the yeast would respire. You remeasured each tube at 30, 60 and 90 minutesand made the appropriate calculations to complete the data table below Use that data tableto answer the question that foil

1. C:°mparing tubes 3 and 4, what specificallIIICO2 production? (1 pt) Yhas occurred to account for the differences

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Solution CO2 produced (ml)a to 3a min 30 to 60min 60 to 90min

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# Solution CO2 produced (ml)0 to 30 min 30 to 60 min 60 to 90 min

1 water + yeast 0 0.1 02 glucose + water + yeast 1.0 2.0 1.53 glucose + NaF +yeast 0.0 0.5 0.04 glucose + MgSO4 + NaF + yeast 1.0 1.5 1.55 glucose + MgSO4 + yeast 1.5 2.5 2.0

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F. ..You and your lab partners have properly set up an apparatus to measure photosynthesis in a10 inch sprig of Elodea. You placed the Elodea into a cup containing water into whichyou breathed parbon dioxide. There is a second cup identical to the first except that itIa,cksthe Elodea plant. You and your partners observe gas bubbles on the Elodea leavesindicating that the plant has indeed photosynthesized substantially.

I. Calculate the amount of oxygen in parts per million (ppm) produced by the Elodea perminute between readings-3and 5. Your answer must be in ppm/min. (l pt)

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2. Calculate the increase in the amount of oxygen in the plain water cup in parts per million(ppm) per minute between readings 3 and 5, Your answer must be in ppmlmin. Why didthis occur? (1 pt) ~ . (/-/ v-J---Rc.1'e. ~.~ ~ 1--5"'~ b'>~ fl7v-.-; d .D!;~,-~ - . ""-

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All 4 tubes contain phosphate buffer, tubes 1 to 3 contain DCPIP, tubes 1,3 and 4 containfresh chloroplasts and tube 2 contains boiled chloroplasts. You record the initial color ofeach tube in your data chart below. Tube 3 is wrapped in aluminum foil, then all 4 tubesare placed in front of a fluorescent light. After 20 minutes you examine all of the tubesand record the following colors. (2 points total)

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1. Ex,PJamwhy the final color o~tube #2 differs from that of tube #1. (lpt) .

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Water+No Elodea Water+ ElodeaReading Time Reading (ppm) 02 Evolved(m!) Reading(ppm) 02 Evolved(ml)I 9:40 6.5 6.52 9:45 6.5 6.83 9:50 6.8 7.84 10:00 7.2 8.55 10:10 7.5 11.86 10:30 7.9 13.8

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2. Did the chloroplasts in tube #4 do the Hill reaction? Explain why or why not. (lpt)-1.~ J b-t,Cc1-LL./~( ,'+ do,€.;...,j co",h,:'r- 'l)Cpl/, tutA.;,.L (:,~ t. /(;'!,!;~ ~

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chlorophyll a = 0.6xanthophyll= 0.7

chlorophyll b = 0.4carotene = 0.95

1.Which spot on the chJ:omatogramshown belolw is most likely chlorophyll a? (I pt)

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I. 1.A cell contains different amounts of DNA at various times in its celi cycle. At

exact!Y-whi~tage does the ploidy double from 2N to 4N? Explain ~our., aasWer. (1 pt) ( , ~ '. 1./ .', I

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2.Why is it important that the chromosomes line '

in mitotic metaphase? (2 pts) up along the metaphase plate

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3.Would a population of organisms well-suited to a very stable environmentwant to reproduce via mitosis or meiosis? Why? (2 pts)

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4.You havejust learnedaboutMendeliangeneticsand the Punnettsquare. Thefirst step in workingout a cross is determiningwhatkinds of gametescanbeproducedby each parent. Your lab partnerhas donethis for the firstparentthat is heterozygousforone trait andhomozygousfor the other(AABb)obtainingthese gametes: AAandBb. Givenyourknowledgeof theprocessof meiosis, is your labpartner's answerright,partlyright orwrong? Explainin termsofmeioticevents! (2 pts),-\~ D-v-SU-J-e..-'("S LJYO4 .

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J. You and your lab partner have discovered a new animal -a felger -thatresembles a deer in the head and tail and a horse in the body. They come in twocolors, brown and black, and some have white on their legs up to the knee (youcall these socks). After watching several successive generations offelgersbeing born, you find that when a black, socked felger mates with another black,socked felger, the foals produced are always black with socks. When thesemate together the foals (offspring) are also always black with socks. Similarlybrown, unsocked felgers mated together produce only brown, unsocked felgers.When brown felgets are mated to black ones, the first generation alwaysproduces only black felgers, but when these are mated together the nextgeneration has black and brown felgers in a ratio of 3 to 1. When unsockedfelgers are mated to socked ones, the first generation always produces onlysocked felgers, but when these are mated together the next generation hassocked and unsocked felgers in a ratio of 3 to 1. (Hint: first determine whichtrait is dominant and which is recessive, use the letters B or b to describe colorand S.or s to describe the leg markings) (Mare =breeding female, Stallion=breeding male)What kinds of gametes could be produced by a doubly heterozygous felgermare that is black and has socks? What is this mare's genotype? (2 pts)

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fjP /(You mate a blacf~thout s~cI{sstallion that always produces black babies withoutsocks to a black mare with socks that prog.upesblack and brown babies with andwithout socks. --p- (jb 51

Is it possible thatthe foal born from this mating will be brown and havesocks? Why or why not? (2 pts)

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a. You have discovered that because bf their stark beauty felgers that are black and /) 1without socks can be sold for the greatest amount of money. Being a typicallypoor, but greedy felger breeder, what are the names of the felgers you would pickto be your herd stallion and mare? Only the ones listed above are for sale! You'

can only afford to buy two felgers! (2 pts) 13/31~p

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You and your lab partners perfonned the experiment entitled "Peas, Genes andEnzymes" according to the protocol in your lab manual. Your results are shown inthe diagram above.

1. According to this experimental data which type of pea, smooth or wrinkled,synthesized amylose at th9ster rate? (1;pt)

l'2. Does this confinn your expectations? Why or why not? (2 P.ts)

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3. What reaction has occurred in the other pea powder? (the opposite onefrom youranswerto question#1) Why? (;2pts) , I

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4. What do the reactions at SC-30 and WC-60 indicate? (1 pO

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N. Fill in the blanks: [1- f~ t- eac.hJ -<J. 1. Enzyme spe~ is due to each enzyme's unique

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2. Anaerobjc-~AlcoholicFennentation: 6(CH20) -7 2(CO2)+ 2(C2HsOH)+~ ATP

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3. r.~sorYbtelnorganicsolvents.

4. ChrOm~cined .

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is an accessory photosynthetic pigment that is

5'. A single genetic ttait'eanhave dozens of possible .

I ./(; \ ).() I.{I. . but a nonnal individual can'-possess only two. - .

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6. Theenzyme ~s1:;;':;-d",-- S''-}-Jt\tk-e.~. S-fenhancJs..th€production of unbranched amylose from simple sugars.

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8. A strong bas>l~ k.QJ1carbon~de. produced in the respirometer.

are organic catalysts made

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Y9. Thep!..,h,~- ~~,., ,..L \reaction involvesshuttlingan excitedI ~ . electron throug~o ecules to generate ATP.

lO.#itoti9~ +.e I.')pt~~~ . : chromatids becomeCliffaSechromatin. .

~~L. diploid individual.

: actual genetic makeup of a

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12.---1>N~ codes for the kind and amount of structural and storage

c~Ydfates foundin a seecl.

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O. TrueorFalse:~~.~:r each] .1. Glucose-I-phosphate ISconverted mto amylose and then to amylopectm~/

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2y.p;;;sence of iodine amylose tnrns a reddish-pnrple color. tru@

3. Mendel'~irsL~h chromosomeis segre ed into a gametewithoutany'~uen:ee fromanyQtherchromosome.tru false

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4. Humans p.Ql'l111i11yhave 26 pairs of chromosomes. true jalse~.'

5. . ThecWQ.roPhr~s madeby blendingspinachleaves in a stronghypotonicU.ugafS(;lution.true~

~sis a six-carbon molecnle becomes 3 two-carbon molecules. trueB7. C~H, ~ ion concentrations alter the secondary (2') .l--sttUcture of the enzyme. true~ '

8. Hydrolysis ~Iecules into complex ones by the addition of a water~curet;:;;e ~ .

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~lasts reflect greenwavelength light.@/false

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l~gOUS condition is represented as Aa. true(f!!iJ

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p. Answer the following as indicated:

1. Complete th;JOl~ reaction:,/

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(2 pts)

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2. List (any) 2 factors- pt-l7 I~ ~~.1fZ"i~t..t,...~

at affect enzyme activity: (2 pts)

3. Name any two products of alcoholic fermentation.

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(2 pts)

4. Naffie one compound that is an activator of respiration and one that is an inhibitor ofrespiration. NOTE: Indicate clearly which compound is the activator and which one is theinhibitor! (2 pts)

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. ,,---5. On gainin~leetr6i1DCPIP is y;R: dIAr~) (oxidized/ reduced)an~s c'1JJl~.rJ~ ~s (blue/ blue-green/ colorless)(2pts)

. 6. Photosynthesis is div~r.f)cesses.'Name any two. (2 pts)L,~~t)l'Ti iJ~ ,

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7. List the four stages of mitosis. (1 pt)

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'r~ C;~A } ~8. ABa blood ~~s are an example of incompletedominance/~mina~hY?(~pts) , .!

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9. Wrinkled pea seeds are formed because of a defect in the gene for a particular enzyme.Name that enzyme and the product of the reaction catalyzed by this enzyme. (2 pts)

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Do not vYritebelow this line.Answers written below this line will not be graded and you will lose an additional 5points ifyou do so.

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