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همدقم ب ك تسا باهدور یفنم مرگ برتكاب کی یلكايشیرشا هوالکع برتكاب نیا گدراد لعت سایرتكابورتنا ب هداوناخ ی کكی ناوکنع ب شراوگ هاگتسد رد یعيب رولف شان رب و یکشراوگ و کيوتکسيس باکه تنوفع یلصا ماوع زا ناککهج رککسارس رد ناککسنا و تاککناويح رد رککيم و گرککم و ماد تا دکيلوت رکضاح لاکح رد گ(0) تسا هدش شرازگ عیانکص باکه شکخب نیرکت مکهم زا یکكی ناوکنع ب روي رايسب شان ،ناریا لوج زا اهروشك زا برايسب رد ییاذغ تکمواام زورکب و ازیراکويب یلكايکشیرشا لااتنا رد یوهم عکسوت لاکح رد عکماوج رد اکه ککيتويب یکتنآ کب تبسن گدراد رد صوککصخب اککه کککيتويب یککتنآ زا ککیور یککب هدافتککسا
کك تکسا یلماع نیرت مهم قالاوتحا ،ماد شرورپ تعنص گ(62) دککشاب یککم یكيتوککيب یککتنآ تککمواام روککهي ثککعاب ییاذکغ ب هرکيجنز اکب طبترم تاناويح شرورپ رد بلغا یعيکسو حطکس رد اکه ککيتويب یکتنآ زا یور یب هدافتسا ، دکح زا شيکب ب هدافتکسا نکیا ب جيتن رد و تسا شیار رد ککيتويب یکتنآ ب مواام ییایرتكاب باه براويب موقو مرازکم رد بکلغا گتکسا کتفای شیازکفا رکيخا باکه لاس ماکش لگ ماوت ،اه براويب زورب نامز رد روي یشرورپ یكيتوکيب یتنآ نامرد تحت ،ملاس و راويب تاناويح ماوت اکيغ رد اکه ککيتويب یکتنآ زا هدافتکسا و دنريگیم رارق اه تنوفع اب هجاوم ماگنه ب اه نآ ب التبا زا اه براويب لاکحرد باهروکشك رد کوع نیا گ(2) دنكیم بريگشيپ مويش روي مرازم رد با هدور نژوتاپ روضح ك عسوت گ(01،92) تسا شیار رايسب دراد بدایز Avian ناوکنع تکحت ناگدکنرپ بازیراويب یلكايشیرشا
pathogenic E. coli (APEC) کککی رککضاح لاککح رد ناکهج رکسارس رد روکي تعنکص رد تنوفع مهم ماوع اه یوس نیا ب تسباو بداصتقا باهنایز لاس ره و تسا
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Journal of Veterinary Microbiology, Volume 14, Issue 2, 2018
Antibiotic resistance and detection of the fimH gene among Escherichia coli isolates obtained from human and broilers
1. Assistant Professor, Department of Pathobiology, Faculty of Veterinary Medicine, Amol University of Special Modern Technologies, Amol, Iran
2. Bachelor of Science, Faculty of Veterinary Medicine, Amol University of Special Modern Technologies, Amol, Iran
3. Master of Microbial Biotechnology, University of Shahrekord, Shahrekord, Iran 4. Master of Food Health, Faculty of Veterinary Medicine, University of Chamran, Ahvaz, Iran
5. Ph.D. of Veterinary Microbiology, Faculty of Veterinary Medicine, University of Chamran, Ahvaz, Iran
Received: 10 June 2017 Accepted: 20 May 2018
Abstract Broilers are one of the potential sources of bacterial infections particularly Escherichia coli infections in human. Different studies have previously showed genetic similarities between APEC (avian pathogenic E. coli) strains and human pathogen strains of the microorganism. In this study we evaluate the presence of the fimH virulence gene among human and broiler isolates of E. coli. In addition the antibiotic susceptibility of the strains was investigated. After collection and identification of the isolates (including 26 human isolates and 60 broilers isolates), at first, total DNA of the pure microorganisms was extracted using boiling method and then DNA samples were subjected to PCR using specific primers for fimH gene. Antibiotic resistance pattern was evaluated by disk diffusion methods for 14 different antibacterial agents. Among 86 E. coli isolates, the fimH gene was detected in 53 (61.62%) isolates. In addition, the isolates showed high resistance to oxacilin (93.02%), colistin (91.86%) and cephalexin (89.53%) respectively. According to the results, the prevalence of the fimH gene among human isolates was significantly (P<0.05) higher than avian isolates. In total, 70 different antibiotic resistance patterns were observed among isolates which showed that there are significant differences between the resistance patterns of different isolates with different sources. It can be due to the use of various antibiotics in treating the complications of this bacterium in humans and livestock. Keywords: E. coli, fimH, antibiotic resistance
*Corresponding author: Khoshbakht, R. Address: Department of Pathobiology, Faculty of Veterinary Medicine, Amol University of Special Modern Technologies, Amol, Iran. Tel: +981144271054 E-mail address: [email protected]