Introducing the Automated Fiber Analyzer • Performs Crude Fiber, ADF and NDF determinations • Automatically adds required solutions and hot water rinses • Batch Process up to 24 samples at one time • Accurate and Precise results, time after time • Computerized controls eliminate technician variability The ANKOM 2000 Fiber Analyzer automates fiber determinations. Capable of Acid Detergent, Neutral Detergent and Crude Fiber analysis, the ANKOM 2000 eliminates chemical and hot water handling. The samples are prepared using ANKOM Filter Bag Technology and placed in a bag suspender. At that point the technician simply selects the analytical method on the computer controller and pushes a button. The system automatically adds the appropriate chemicals and performs the necessary rinses. The solution is heated and solublization takes place without manual intervention. When the process is complete, the technician simply removes the bags and completes a final drying before weighing the samples. Helping To Feed The World! ANKOM Technology is the developer of Filter Bag Technology (FBT). Samples are complete- ly encapsulated which insures ease of handling and virtually elimi- nates error. Operator exposure to chemicals is eliminated. Using Filter Bags, productivity soars as samples are batch processed in an automated system. The samples per day your lab can process will dramatically increase. Costs are decreased as your technicians are free to take on other tasks while the ANKOM automated analyzer handles the process. • Accurate Results • Increased Precision • Batch Processing • Lower Costs • High Output Used in over 85 countries around the world, the ANKOM 200 Fiber Analyzer provides a low cost alternative for the laboratory seeking increased efficiency. Technicians simply add the required solutions and hot water rinses according to the method and the ANKOM 200 handles the rest. Also Available
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Introducing the Automated Fiber Analyzer• Performs Crude Fiber, ADF and NDF determinations
• Automatically adds required solutions and hot water rinses
The ANKOM2000 Fiber Analyzer automates fiber determinations.Capable of Acid Detergent, Neutral Detergent and Crude Fiber analysis,the ANKOM2000 eliminates chemical and hot water handling. The samples are prepared using ANKOM Filter Bag Technology and placed ina bag suspender. At that point the technician simply selects the analytical method on the computer controller and pushes a button. Thesystem automatically adds the appropriate chemicals and performs thenecessary rinses. The solution is heated and solublization takes placewithout manual intervention. When the process is complete, the technician simply removes the bags and completes a final drying beforeweighing the samples.
Helping To Feed The World!
ANKOMTechnology isthe developer ofFilter Bag
Technology (FBT).Samples are complete-
ly encapsulated which insures easeof handling and virtually elimi-nates error. Operator exposure tochemicals is eliminated. UsingFilter Bags, productivity soars assamples are batch processed in anautomated system. The samplesper day your lab can process willdramatically increase. Costs aredecreased as your technicians arefree to take on other tasks whilethe ANKOM automated analyzerhandles the process.
• Accurate Results• Increased Precision• Batch Processing• Lower Costs• High Output
Used in over 85 countries around the world, theANKOM200 Fiber Analyzer provides a low cost alternative for the laboratory seeking increased efficiency. Technicians simply add the required solutions and hot water rinses according to the method and the ANKOM200 handles the rest.
SpecificationsSample SpecificationsSample Size 0.5 – 1.0 gramFiber Range: 0% - 100%Samples per batch: up to 24
Samples per day Acid Detergent Fiber = 144Neutral Detergent Fiber = 120 Crude Fiber = 96
Instrument Specifications: ANKOM2000 ANKOM200
Dimensions: 18”w x 14.5”d x 23”h 16”w x 9”d x 20”hOperating Temperature 100°C 100°CPower Requirements 120 – 240V, 50/60 Hz 120–240V, 50/60 HzWeight 46 kg. 20 kg
ANKOM Technology is the developer of Filter Bag Technology (FBT) used aroundthe world for fiber and fat analysis. With customers in over 85 countries, ANKOMhas a reputation for quality and innovation. Constantly seeking to develop bettermethods for time consuming analytical methods, ANKOM Technology focuses oncustomers needs. We offer instruments, chemicals and other ancillary products tosupport fiber studies, crude fat extraction, total fat extraction, in vitro and in situresearch and more. We work hard to keep costs low with quality and service high.
The ANKOM2000 Fiber Analyzer automates fiber determinations.Capable of Acid Detergent, Neutral Detergent and Crude Fiber analysis,the ANKOM2000 eliminates chemical and hot water handling. The samples are prepared using ANKOM Filter Bag Technology and placed ina bag suspender. At that point the technician simply selects the analytical method on the computer controller and pushes a button. Thesystem automatically adds the appropriate chemicals and performs thenecessary rinses. The solution is heated and solublization takes placewithout manual intervention. When the process is complete, the technician simply removes the bags and completes a final drying beforeweighing the samples.
Helping To Feed The World!
ANKOMTechnology isthe developer ofFilter Bag
Technology (FBT).Samples are complete-
ly encapsulated which insures easeof handling and virtually elimi-nates error. Operator exposure tochemicals is eliminated. UsingFilter Bags, productivity soars assamples are batch processed in anautomated system. The samplesper day your lab can process willdramatically increase. Costs aredecreased as your technicians arefree to take on other tasks whilethe ANKOM automated analyzerhandles the process.
• Accurate Results• Increased Precision• Batch Processing• Lower Costs• High Output
Used in over 85 countries around the world, theANKOM200 Fiber Analyzer provides a low cost alternative for the laboratory seeking increased efficiency. Technicians simply add the required solutions and hot water rinses according to the method and the ANKOM200 handles the rest.
SpecificationsSample SpecificationsSample Size 0.5 – 1.0 gramFiber Range: 0% - 100%Samples per batch: up to 24
Samples per day Acid Detergent Fiber = 144Neutral Detergent Fiber = 120 Crude Fiber = 96
Instrument Specifications: ANKOM2000 ANKOM200
Dimensions: 18”w x 14.5”d x 23”h 16”w x 9”d x 20”hOperating Temperature 100°C 100°CPower Requirements 120 – 240V, 50/60 Hz 120–240V, 50/60 HzWeight 46 kg. 20 kg
ANKOM Technology is the developer of Filter Bag Technology (FBT) used aroundthe world for fiber and fat analysis. With customers in over 85 countries, ANKOMhas a reputation for quality and innovation. Constantly seeking to develop bettermethods for time consuming analytical methods, ANKOM Technology focuses oncustomers needs. We offer instruments, chemicals and other ancillary products tosupport fiber studies, crude fat extraction, total fat extraction, in vitro and in situresearch and more. We work hard to keep costs low with quality and service high.
HORRAT VALUE 3.07 1.14 1.18 1.75 1.82 1.11 0.62 1.42 1.83 1.75 6.34a Official Method AOCS Ba 6-84/AOAC 962.09
Table 2. Summary of the statistical analysis of the Filter Bag Technique crude fiber collaborative study, including
comparison with the Official Method.
Method 8 – determining Acid Detergent Lignin in beakers
3/13/13 pg. 1 of 2
Reagents
Sulfuric acid (72% by weight) – ANKOM Technology FSA72 or dilute reagent grade H2SO4 to a specific
gravity of 1634 g/L at 20°C (24.00N) by adding 1200 g H2SO4 to 350 ml H2O in a 1 L MCA volumetric flask
with cooling. Standardize this solution to 1634 g/L at 20°C specific gravity by removing solution and adding
H2O or H2SO4 as required.
Safety Precautions (see attached MSDS)
WARNINGS:
Acetone is highly flammable. Use fume hood when handling acetone and
avoid inhaling or contact with skin. Ensure that all the acetone has
evaporated before placing in the oven.
Wear rubber gloves and face shield when handling sulfuric acid. Always
add sulfuric acid to water. If acid contacts skin, wash with copious
amounts of water.
Apparatus
a) Filtration device – ANKOM Technology – F57 Filter Bags
b) Impulse bag sealer – ANKOM Technology – 1915 Heat Sealer
c) Desiccator – ANKOM Technology – Desiccant/MoistureStop pouch – X45
d) 2L & 3L Beaker
Procedure 1) Grind the sample to pass through a 1 mm screen (2mm screen when using a cyclone mill).
2) Weigh each Filter Bag (W1), record the weight, and tare the balance.
3) Add 0.5 g (±0.05 g) of air-dried sample (W2) directly into each Filter Bag.
4) Weigh and seal one (1) blank bag and include it in the digestion to determine the blank bag correction
(C1).
5) Seal the bags closed 4 mm from the open edge using the heat sealer.
6) Spread the sample uniformly inside each filter bag by flicking the bag to eliminate clumping.
7) Perform ADF determinations using Fiber Analyzer (See ADF Procedure).
8) After performing ADF determinations, place dried bags with samples into a 3 L beaker and completely
cover the bags with 72% H2SO4 (approximately 250 ml).
Bags must be completely dry and at ambient temperature before adding
concentrate acid. If moisture (even ambient moisture) is present in the
bags, heat generated by the H2SO4 and H20 reaction will adversely affect
the results.
9) Place a 2 L beaker inside the 3 L beaker to keep bags submerged. Agitate the bags at the start and at 30-
minute intervals by gently pushing and lifting the 2 L beaker up and down approximately 30 times.
10) After 3 hours pour off the H2SO4 and rinse with tap water to remove all acid.
If acid remains in the bags when they go into the oven, the samples will
burn, resulting in values that are higher than they should be.
11) Repeat rinses until pH paper shows neutral color when touching the bags. Rinse with approximately 250
ml of acetone for 3 minutes to remove the water. Handle the bags gently during rinsing. Fine lignin
particles can exit the filter if not handled carefully.
Method 8 – determining Acid Detergent Lignin in beakers
3/13/13 pg. 2 of 2
12) Dry the bags in an oven at 105 C for 2-4 hours.
WARNING – Do NOT place bags in an oven until all acetone has evaporated.
13) Remove the bags from the oven and place them directly into Desiccant/MoistureStop pouches and flatten
to remove air. Cool to ambient temperature and weigh the bags (W3).
14) Prepare each bag for the ash procedure. Extra Material
14.1) Fold each bag from bottom to top. Because the bags are wider at the
top than at the bottom, there will be a little extra material on each side
after the first fold.
14.2) Fold each bag from right to left. The extra material now lines up on
left side.
Extra Material
14.3) Heat seal the bag at the location of the extra material.
15) Ash the bags in pre-weighed crucibles (30 or 50 ml) at 525 C for 3 hours or until C-free. Cool and
calculate weight loss (W4).
16) Calculate blank bag ash correction (C2) using weight loss upon ignition of a blank bag sequentially run
through ADF and lignin steps.
17) Calculate percent ADL.
Where: W1 = Bag tare weight W2 = Sample weight W3 = Weight after extraction process W4 = Weight of Organic Matter (OM) (weight loss on ignition of bag and fiber residue) C1 = Blank bag correction (final oven-dried weight/original blank bag weight) C2 = Ash corrected blank bag (Loss of weight on ignition of bag/original blank bag)