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Fasting-Refeeding Impacts Immune Cell Dynamics and Mucosal ...cbdm.hms. · PDF file Glut1 depletion leads to decreased B cell number and antibody production, glycolytic rewiring is

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  • Article

    Fasting-Refeeding Impacts Immune Cell Dynamics

    and Mucosal Immune Responses

    Graphical Abstract

    Highlights

    d Fasting drastically reduces lymphocyte levels in Payer’s

    patches

    d Naive B cells migrate to bonemarrow during fasting and then

    back upon refeeding

    d Nutritional signals are essential to maintain CXCL13

    expression by stromal cells

    d Fasting causes GC B cell death and attenuates antigen-

    specific IgA response

    Nagai et al., 2019, Cell 178, 1072–1087 August 22, 2019 ª 2019 Elsevier Inc. https://doi.org/10.1016/j.cell.2019.07.047

    Authors

    Motoyoshi Nagai, Ryotaro Noguchi,

    Daisuke Takahashi, ..., Keiyo Takubo,

    Taeko Dohi, Koji Hase

    Correspondence [email protected]

    In Brief

    Temporary fasting drastically reduces the

    levels of B cells in Peyer’s patches, with

    germinal center B cells undergoing

    apoptosis and naive cells migrating to the

    bone marrow and only egressing upon

    refeeding.

    mailto:[email protected] https://doi.org/10.1016/j.cell.2019.07.047 http://crossmark.crossref.org/dialog/?doi=10.1016/j.cell.2019.07.047&domain=pdf

  • Article

    Fasting-Refeeding Impacts Immune Cell Dynamics and Mucosal Immune Responses Motoyoshi Nagai,1,2 Ryotaro Noguchi,1,2 Daisuke Takahashi,1 Takayuki Morikawa,3 Kouhei Koshida,1 Seiga Komiyama,1

    Narumi Ishihara,1 Takahiro Yamada,1 Yuki I. Kawamura,2 Kisara Muroi,1 Kouya Hattori,1 Nobuhide Kobayashi,1

    Yumiko Fujimura,1 Masato Hirota,1 Ryohtaroh Matsumoto,1 Ryo Aoki,4,5 Miwa Tamura-Nakano,6 Machiko Sugiyama,2,7

    Tomoya Katakai,8 Shintaro Sato,9,10 Keiyo Takubo,3 Taeko Dohi,1,2 and Koji Hase1,10,11,* 1Division of Biochemistry, Faculty of Pharmacy and Graduate School of Pharmaceutical Science, Keio University, Tokyo 105-8512, Japan 2Department of Gastroenterology, Research Center for Hepatitis and Immunology, Research Institute, National Center for Global Health and

    Medicine, Chiba 272-8516, Japan 3Department of Stem Cell Biology, Research Institute, National Center for Global Health and Medicine, Tokyo 162-8655, Japan 4Division of Gastroenterology and Hepatology, Department of Internal Medicine, Keio University School of Medicine, Tokyo 160-8582, Japan 5Institute of Health Sciences, Ezaki Glico Co., Ltd., Osaka 555-8502, Japan 6Communal Laboratory, Research Institute, National Center for Global Health and Medicine, Tokyo 162-8655, Japan 7Laboratory for Immunobiology, Graduate School of Medical Life Science, Yokohama City University, Kanagawa 230-045, Japan 8Department of Immunology, Graduate School of Medical and Dental Sciences, Niigata University, Niigata 951-8510, Japan 9Mucosal Vaccine Project, BIKEN Innovative Vaccine Research Alliance Laboratories, Research Institute for Microbial Diseases, Osaka

    University, Osaka 565-0871, Japan 10International Research and Development Center for Mucosal Vaccines, the Institute of Medical Science, the University of Tokyo (IMSUT),

    Tokyo 108-8639, Japan 11Lead Contact

    *Correspondence: [email protected] https://doi.org/10.1016/j.cell.2019.07.047

    SUMMARY

    Nutritional status potentially influences immune re- sponses; however, how nutritional signals regulate cellular dynamics and functionality remains obscure. Herein, we report that temporary fasting drastically reduces the number of lymphocytes by �50% in Peyer’s patches (PPs), the inductive site of the gut immune response. Subsequent refeeding seemingly restored the number of lymphocytes, but whose cellular composition was conspicuously altered. A large portion of germinal center and IgA+ B cells were lost via apoptosis during fasting. Meanwhile, naive B cells migrated from PPs to the bone marrow during fasting and then back to PPs during refeeding when stromal cells sensed nutritional signals and upregulated CXCL13 expression to recruit naive B cells. Furthermore, temporal fasting before oral immunization with ovalbumin abolished the induc- tion of antigen-specific IgA, failed to induce oral tolerance, and eventually exacerbated food anti- gen-induced diarrhea. Thus, nutritional signals are critical in maintaining gut immune homeostasis.

    INTRODUCTION

    Inappropriate calorie intake is a global health problem. In devel-

    oping countries, the nutritional deficiency often compromises

    vaccination efficacy and increases the risk of infectious dis-

    eases (Kaufman et al., 2011; Savy et al., 2009; Scrimshaw and

    SanGiovanni, 1997). Furthermore, childhood malnutrition is a

    predisposing factor for environmental enteropathy characterized

    by intestinal dysfunction, increased intestinal permeability, and

    microbial dysbiosis (Brown et al., 2015; Humphrey, 2009). In

    industrialized countries, on the other hand, excessive food intake

    accompanied by a lack of exercise has augmented the incidence

    of obesity (World Health Organization, 2016), which is a signifi-

    cant risk factor for cardiovascular disease, metabolic syn-

    dromes, and cancer (Basen-Engquist and Chang, 2011; Grundy,

    2004; Poirier et al., 2006). Low-grade inflammation due to

    obesity is significantly implicated in the development of these

    diseases (Visscher and Seidell, 2001). These observations

    indicate that nutritional status has a significant impact on the

    immune system.

    The gastrointestinal mucosa is directly exposed to exogenous

    food ingredients and thus inevitably faces drastic changes in the

    nutritional status of the lumen during food uptake and fasting.

    We previously demonstrated that intestinal tissue is highly sus-

    ceptible to deprivation of luminal nutrients, as temporal fasting

    arrested epithelial cell proliferation while refeeding induced hy-

    perproliferation in the intestinal epithelium (Okada et al., 2013).

    Given that epithelial cell turnover constitutes a robust first-line

    barrier to external antigens, mucosal barrier function may be

    more vulnerable during fasting than during feeding. Considering

    that fasting relieves the burden of food-borne antigens and

    microorganisms on the gut mucosa, it is thus reasonable to

    decelerate epithelial cell turnover temporarily to minimize energy

    expenditure under nutrient deprivation.

    The gut mucosal barrier consists of not only intestinal epithe-

    lium but also an underlying immune system that establishes the

    second-line barrier. The gutmucosal immune response is charac-

    terized by the production of dimeric or polymeric immunoglobulin

    1072 Cell 178, 1072–1087, August 22, 2019 ª 2019 Elsevier Inc.

    mailto:[email protected] https://doi.org/10.1016/j.cell.2019.07.047 http://crossmark.crossref.org/dialog/?doi=10.1016/j.cell.2019.07.047&domain=pdf

  • A

    B

    C

    D E

    Figure 1. Characterization of PPs during Fasting and Refeeding

    (A) Serial sections of PP were stained with H&E. PPs were obtained frommice fed ad libitum (left), fasted for 36 h (middle), or refed with CE2 for 48 h (right). Scale

    bar, 200 mm.

    (B) Immunostaining of PPs from mice fed ad libitum (left), mice fasted for 36 h (middle), and mice refed with CE2 for 48 h (right). Scale bar, 200 mm.

    (legend continued on next page)

    Cell 178, 1072–1087, August 22, 2019 1073

  • A (IgA) to the mucosal surface (Lycke and Bemark, 2017).

    Secretory IgA (S-IgA) plays vital roles in host defense against

    pathogens, inhibition of microbial metabolite penetration, and

    regulation of the gut microbial community (Mantis et al., 2011;

    Uchimura et al., 2018; Wei et al., 2011). To efficiently induce

    S-IgA response, luminal antigens are actively taken to gut-associ-

    ated lymphoid tissue, such as Peyer’s patches (PPs), that serve

    as an inductive site of mucosal immunity. In PPs, germinal center

    (GC) reactions, namely, class switch recombination to IgA as well

    as affinity maturation, occur continuously with the aid of follicular

    helper T (Tfh) cells. IgA class-switched B cells subsequently

    egress PPs and then home to the intestinal lamina propria via

    mesenteric lymph nodes (MLNs), the thoracic duct, and blood

    circulation, during which IgA+ B cells terminally differentiate into

    IgA-producing plasma cells.

    Multiple lines of research have uncovered a link between im-

    mune cell function and metabolic status (Kau et al., 2011; Man

    and Kallies, 2015). For example, upon T cell receptor (TCR) stim-

    ulation, effector T (Teff) cells enhance the uptake and utilization

    of glucose to promote aerobic glycolysis. Activated Teff cells

    also upregulate glutaminolysis. Such metabolic reprogramming

    is essential for Teff cells to meet the energy demand of clonal

    expansion and effector functions, such as the production of

    inflammatory cytokines (Carr et al., 2010). Furthermore, IgA+

    plasma cells in the intestine preferentially utilize glycolysis for en-

    ergy metabolism, whereas naive B cells in PPs usually gain ATP

    through aerobic metabolism in mitochondria (Kunisawa et al.,

    2015). Stimulation with lipopolysaccharides (LPS) or B cell re-

    ceptor (BCR) ligation upregulates glucose transporter 1 (Glut1)

    expression in B cell activating factor (BAFF)-pretreated B cells,

    which eventually undergo metabolic reprogramming to glycol-

    ysis (Caro-Maldonado et al., 2014). Because B cell-specific