2018/10/19 Genovis » FabRICATOR-HPLC https://www.genovis.com/products/igg-proteases/fabricator/fabricator-hplc/?utm_source=Digested+Newsletter+Genovis&utm_campaign=4de13a… 1/10 Genovis / Products / IgG Proteases / FabRICATOR / FabRICATOR-HPLC FabRICATOR ® -HPLC FabRICATOR-HPLC is an HPLC column for fast on-column digestion of monoclonal antibodies. The cysteine protease FabRICATOR (IdeS) immobilized on the column delivers rapid and consistent subunit generation, perfect for automated online analysis in a middle-level LC/MS workflow. Products Detailed Information Key Characteristics How to Order Documents FAQ & Support Patent & Disclaimer Applications We use cookies to ensure that we give you the best experience on our website. If you continue to use this site we will assume that you are happy with it. Ok Ok Leave a message Leave a message Leave a message Leave a message Leave a message Leave a message Leave a message Leave a message Leave a message Leave a message
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FabRICATOR -HPLC » FabRICATOR... · The performance of the FabRICATOR-HPLC column at 37°C is typically >200 injections with a digestion efficiency of >95% for 10 µg human IgG1
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FabRICATOR®-HPLCFabRICATOR-HPLC is an HPLC column for fast on-column digestion of monoclonalantibodies.
The cysteine protease FabRICATOR (IdeS) immobilized on the column delivers rapid andconsistent subunit generation, perfect for automated online analysis in a middle-level LC/MSworkflow.
Products
Detailed Information
Key Characteristics
How to Order
Documents
FAQ & Support
Patent & Disclaimer
Applications
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assume that you are happy with it. OkOk
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The FabRICATOR-HPLC column with immobilized FabRICATOR (IdeS) enzyme digests IgGrapidly and specifically just below the hinge, generating F(ab’)2 and Fc fragments without anyrisk of over-digestion. The FabRICATOR-HPLC column is designed as an analytical tool forrapid on-column digestion in middle-level workflows for characterization of antibodies andFc-fusion proteins. After digestion, the fragments can be analysed using reversed phaseHPLC, mass spectrometry or other analytical method. Automation of antibody digestion andsubunit generation enables applications in online monitoring of bioreactors, for example aspart of a 2D-LC-MS setup, coupling the reactor directly the MS. This automated approach toantibody subunit analysis reduces operator time, sample handling and increases thethroughput. Leave a message Leave a message Leave a message Leave a message Leave a message Leave a message Leave a message Leave a message Leave a message Leave a message
In-house performance testing* of the FabRICATOR-HPLC column highlights the stability andreproducibility, for complete data see here. The column delivered consistent digestion for 14days at 37°C. More than 450 injections of mAb were made during testing and no carry-overwas observed. Reproducible Fc glycan profile analysis was obtained showing the column tobe suitable for online process monitoring. Modified Fc glycans on a therapeutic mAb werealso detected using FabRICATOR-HPLC fragmentation followed by LC-MS.
Learn more about the applications of FabRICATOR-HPLC
* Tested with trastuzumab in PBS, injections every 40 minutes, at a flow rate of 25 µl/min, samples
collected, reduced and analyzed by RP-HPLC.
Storage
Store the column at +4-8°C in 10 mM sodium phosphate, 140 mM NaCl, pH 7.4 when not inuse. Store the column with the endcaps in place, carefully sealed to prevent drying. For long-term storage of the column 0.02% (w/v) sodium azide can be added as apreservative.
Performance The performance of the FabRICATOR-HPLC column at 37°C is typically >200 injections witha digestion efficiency of >95% for 10 µg human IgG1 (1 mg/ml) sample in PBS, pH 7.4.
Product Overview
Column dimensions: 2.1 mmD x 50 mmL Support resin: POROS™
A0-FRC-050
FabRICATOR-HPLC
€1,599.00
Add to cart
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Typical flow rate: 0.025-0.05 mL/min Maximum Pressure: 100 bar Operating pH: 6.5 - 8.0 Operating temperature: 37°C Storage conditions: +4°-8°C
Number of days of continuous operation: >10 * Injections per column: >200 *
Start material: Human IgG1-4, Fc-fusion proteins
* Depending on specific application
Download Poster
Download CASSS MS Poster”Rapid On-column Monoclonal Antibody Subunit Generation for Automated Glycan Analysis”
Applications
Human IgG1-4, IgG from monkey, rat, rabbit and sheep
Over 95% digestion of 10 ug IgG in less than 5 minutes
FabRICATOR-HPLC Column for automated subunit analysis
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Complete digestion of 10 ug IgG in less than 5 minutes•
No carry-over observed in testing•
Reduce manual sample handling errors•
Increase throughput of subunit analysis•
FabRICATOR-HPLC•
FabRICATOR-HPLC•
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This product is provided under an intellectual property license from Life Technologies Corporation. The
transfer of this product is conditioned on the buyer using the purchased product solely in research or for
analytical testing during manufacturing, all conducted by the buyer, excluding contract research or any fee
for service research, and the buyer must not (1) use this product or its components for (a) diagnostic,
therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for
FabRICATOR-HPLC•
What is the FabRICATOR-HPLC?
Can I use FabRICATOR-HPLC in a 2D-LC setting?
How many samples can I run through a column?
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