• Extremophiles These microbes thrive zmder co,rditioflS ,ha! would ki/l other creatures. The mo/ecu les Ihot enable extremophiles to prosper aTC becoming useful ta industry -. 1 =Il"" di'UlIlnlO • cool .... p<lOl. N<>w, INnk ""qud of plowtnc inIo ....lff dur IJ boil ... « lIeU &CUlIWo OranMln jwnfJir"l! in", """"la Of ClIll«IlIr.IlN brino, Tht IclIp ... 'OOI.l .... dil.asfro", for • pn;oJD. Yet ...... mak.w........... in sudl btli.ld;rt; m.irom•• "", n...c, minot... In: albJ t- c.:>.... rhq rhn !rom Ibo h arc c"'rly lX' tr=I<. Amuintll" th< OfF n...... do """ .... mr lbrir "": lh<y do 11<;0 id rholr ""bit....nd, IR ""'''1 <;:lI"'" Iftl""" ........ """'" UfrtmoS in Somr G1"'Ii .... ho,.., brm kmwa for ""'" 40 lIIll: dt< <dI ",,".Ih<m ha> ... 1mUIi<d ...,...rl:< . ........ Iu." m.ngruud m_n orn • ........ ,j 10 br 0lUiIt ,,'ith mi· cn"1lU1 61.. n.. buno tu. .ho boa lu· dnl .. doc P"f ........1fUt' br iMuf. IJY) ruliz.... ,," rIut th< - .........1k... • u.uy _ in .... ruyci.pp1anon •• Of pani<:ular inl=M .... me mzfmtO (bOoL." 01 <::Ill>1r->1 du, hq:> <Xba..... p/i.les 10 tuncno.llll bnPl tirtumIt;I...,. n. likc: .f1lth<tic clI<llf>O, .... """,,,h an: l'''''..".... <perd up chom .....1 ."""il,,, ...ittlolll bol,. .lfefnl tilt.. ..ton. .... hkwi&d....IIirId.DIl odwr lnd\IlllM .. "O<Idwidt '1'tI" !l'lOft lIun SU 1>ilI....... <ntym<S F.. appli. a ....... rllll"'llirom rht l'''nd ni ,,",alOi"" 1O m. F"f"C odm""",-",,," of cnnurulo one! dw di"i"""" of r.ftmouo on<! p- n<ric Y<t ><:tn<hrd mI)""C> ...... .... tm, whm 10 h ... or <JI""" .>ol,........ ..., "" ",.n.u.."Nm> ..... , rdv nn doom ...... cim. ... "" <pr' "",lm,,, ro p«n<1 dw l""'ftM do ...... mo<! ...... Of ..... "'Il!'. Br """"'''''4I,K- nv. "",", C"'''''<ttlJ'I''" """,,1.1 .... .ymos fn:Im ""mdOllll'lol ,'obbN putontulv mm_ ...... dw .... v b mr... addoJ '"""" s CopJn"" .m "' .... ", ...... oncl rnNc- Ïl'I"'-' Thoyaon.hobmm. ...... of mlirdy new <t\lyrM-blIlol'd jICOC<>W$. us.. J'p.n, G<rmocIy oad '" _ ....... ny 1iCI""""" li. nh<l'''if''liob and cll<it only • i<w D_ h.J •• modo ,hrif _ inru .... If,.,. flr, ........ 1<:>""""". "" io ttW ofIWJducl_rmtt. lnIrlliocm· "" a _Iy Id.1ld inrn ...... bIe p ......... lior ......... ,. can ,.h SC\'.nl }'aD. SoucI .... of Oh"'"",*," """" .h<> hrlronl mlnw dw MlIuIiuul'l' lm' of N ... mil:. do..,.. hdd ""', ..... """""'" CtIl>ld ... ...... ,.., imo !WtI ho- oie ..........., lateril, timplemlo Iodt. n...icw, and 'Y"o ......... odIo ""...".,.. complu. The _ wotIr.ltnd. .... "'Il ....",.,.. n, .... ..... , "".......1 pre>- ...... 1 m.. 1 .... ft rhonI """'" m.... m.-. <XDf<. AN,,,,,,,,.IIy••..:n..... lad< • .................Io!IcIy "bi< oth<t,.,.,... NId .m rdIM.1 pnn h.J." u.........""fI"' ho.:lnia. ft 5lII" dw "'" P""f"O Iunrnoa ......
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Extremophiles - Bienvenue | Département de génie ... dirigees/AL_LD1... · Extremophiles. These microbes thrive zmder co,rditioflS ,ha! would ki/l other creatures. The mo/ecules
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bull
Extremophiles These microbes thrive zmder corditioflS ha
would kil other creatures The moecu les Ihot enable extremophiles to prosper
IJ boillaquo lIeU ampCUlIWo OranMln jwnfJirl in Drp~~ ~mshy
la Of ClIlllaquoIlIrIlN brino Tht IclIp OOIl dilasfro for bull pnoJD Yet mmr~n makw in sudl btlildrt mirombullbull nc minot In albJ ~lo t shycgt rhq rhn ~COlldocircrialSm rom Ibo h arc crly lX
tr=Ilt Amuintll thlt OfFn do mr~ lbrir lhlty do 11lt0 id rholr PUIII~ bitnd IR 1
ltlI Iftl UfrtmoS in ordtflO~~Id
Somr G1Ii ho brm kmwa for 40 ~I$ lIIll dtlt ltdI Ihltm hagt 1mUIiltd rllt Iumngruud m_n ~ orn bull j 10 br 0lUiIt ~bltM ith mimiddot cn1lU1 61 n buno tu ho boa lumiddot dnl doc Pf 1fUt br iMuf IJY) ruliz rIut thlt - 1kbull ~~~ltlllpotnl
uuy _ in ruycipp1anonbullbull Ofpaniltular inl=M me mzfmtO
(bOoL 01 ltIllgt1r-gt1 du hqgt ltXba
piles 10 tuncnollll bnPl tirtumItI n likc f1lthlttic clIltllfgtO ~mn
h an l ltperd up chom 1ilittlolll bol lfefnl tilt ton ~-r hkwiampdIIirIdDIl odwr lndIlllM OltIdwidt 1tI llOft lIun SU 1gtilI ltntymltS F appli a rllllllirom rht lnd ni alOi onltI~- 1O m FfC odm- of cnnurulo one dw dii of rftmouo onlt pshynltric ~ Yltt gtlttnlthrd mI)Cgt
tm whm ~ 10 h or ltJI gtol nuNmgt rdv nn doom cim ltpr lm ro plaquonlt1 dw lftM do molt Of Il Br 4IKshynv CltttlJI 11 foo~ ymos fnIm mdOlllllol obbN ~clturmiddotmltI ~-om putontulv mm_ dw v b mr addoJ
sCopJn m
mmy~dicicny oncl rnNcshyIumllI- Thoyaonhobmm of mlirdy new lttlyrM-blIlold jICOCltgtW$
~2(I~pwpc us Jpn GltrmocIy oad ~ _ ny 1iCI li nhltlifliob and cllltit ~Ah~ only bull iltw D_ l~rmegt hJ bullbull modo hrif _ inru If flr ~ 1ltgt io ttW ofIWJducl_rmtt lnIrlliocmmiddot a _Iy Id1ld ~ inrn bIe p lior can h SCnl aD
SoucI of Oh hltgthrlronl mlnw dw MlIuIiuull lm of ~rc N mil do hdd ~ CtIlgtld imo WtI ho-oie lateril timplemlo Iodt nicw and Yo odIo complu The _ wotIrltnd Il n 1pregtshy1m 1 ft rhonI mm- ltXDflt ANIIybullbulln ladlt bull IoIcIy bilt ~nothltt NId m rdIM1 pnn hJ ufI holnia ft 5lII dw PfO Iunrnoa
PlINlSI-lLlG fNVIRONMINT5 1ft bull ~ n ht-r-d 1000lt_ dtpicted
H middot ~middot~ phi III -Dl - ondiod
ol utraIIogtpIugraveln l1Mml gtoJ ur tUdilr Iollmlpmmiddot mres ~a mll 4$ cnw II Il dovltn Flvmhm and ltli rhrm t=d hl~ ro lomIp--3- 10 dosr- C (116 q FI Sltwnr h~ ph ~- lhm Il 11_ 100bull ln Iwo 100 ltleyeltr CIll drrHt f 1Iw hagrave-l POO nl ~ ~bulleo ri III pgtrioœ prdm]Inlttv hamshyrU puw fau in lUffJ -m 1J and lt10 ltltlIren C (77 -J 104 ~ Ft r~ no mllulJ 1gt pLul h~C hmI foonJ 0 NImiddot 3~ rltrm-n ~hobullbullbullbu sa LIemiddot c (lU sr- flbullbullnd _ bul fi d~-I Cln roIn-
bull rm 1lfIampU m remrrnNlllJher dwI bout 6(1 dqren C (HO ~awenI penlWD fi 10 6(1 LIepeo C hu-n a- b bur plusmn 10 lwmtI in aream hul-wftlt lino d ~ _boat JO JU n D Idlt motltd ampc l1nIlt-a of IV _~- op 1__ ml m fPlaquogt_ ltJuriaC bull ~-ltcna -=rob1 61on hot ~ oi NoaonoI Pul W~
no __p iDuDd 1Iw _
- PU1ed Iik III 1 196Oo liwy drnnMdhe for ~aplblr ofYOolW~ mpertumo JlegtaMa ro~Cb_J-1n__
Itd TMnltl dlgtI wouW btct mak _ibIe iltItopKld ltli 3 rr ord (ltIlo met dIol gt(lgtlft (PCItl buuc lbagt me Ilme thlt am kgtUDIi rhltint hy 10 Il D Il hound ltOdic_ l1ul 4 dwnhgtemiddot
CopyrlaIll lm Sdonllllt uMrilt
0 sJ(uIuln tUltinu IW pllfiaRy bullbull ptmNfft -1IiP _ as ~ c n ~ 1 anI con ht pr-ricircn boiIinf shy
Iltodlt (0Il(1IJeJ rom lIw (urin ~ Iueterio funcrion II
JNhelt ptUlU hao ovlcol andpowJlhor~_kl
lilltdr bo id ~ter ~ Otheo 11 iltduJIcircolI h thoo __ m ure 1971llt k ICishy_ fO mon J on -J hyIrothnshy1 -m lIIOnS 10 H iliiIl_ ctIcd -_ 1Ilaquol1 dmn radlt cNnwyo ltwuIh 1UdI mopI pedlnlfd aoirInraquorido lluod bullbull hot )JO-c
10- mon +0 saae al hy pmhomaogtphib lu hem -wd -r~O s-n cUshy~ mUao-yal~
~~~-= 11 Icircltl rhc h al Ir rqgtOOshy
SclDltTlIlC AgtoUICUi AIriII l
bull
duca loe Ih ltJi oOouf lOf ~CIaJ1 nllpIy __ p al P 1U dcpel C Ileshy~IcircI PO lf_bull t1ftI bdow 10 ~C (lM shyf1 Il ltllI1d1 -t-Ioypathctshyophie lNllmJ dup tel dlim~ fiIo _ -pInriclaquoœ-o M 0001) _ dro mld1 ana Iiooo il litI dot fOOIl il the of Iiir anaIyoio ni aod Iioilif if UpenN 10 btlp dariIr how
Noe Too Hoc III H-IIo
R-- 1Cfed fiIo -bOre oi 1 IcircIlI shy
IcircII rirr _ ff71IlIlOdrncand how in Iorinamp -roba lld odoltr umpu io fwlcoOlUI r - lNbull odoolOd biolP bull~ ~
_pcnrr CUumlIHL 1111 il shydolt _ft o1dlouch IcircI _ _
~I diHu -l Ir dn For lltUlIIl bUl-1ooonIIl afnlll shy0I1fIle$ raaDbk lthoir beo-ocircoodonnl
dw _ O J _I-a 1r1Ugraveu middotoct io -- ~cl)lkal nIo il -IimI fi (- bull for HJV ~Rd~ - ru FOcirc OIOItIepacircbility 10 _____bull 1-shy shy
In lC1 - DNA poIltUJIOcirclIlur1onipptt01 DNA prodllQ 0__ p p)l Tho _011 roiaor 10 lIecyccd Ioorwcco lowarwl _W1IaoMshylion iol -l ~ohcpoty-- _ Ire- aobct tIlIf bullbulln ROC l Licnd 00 0f01lltd kmiddot
lbc Iwx plll 01 plcnd~bull Tlaquohnitwu hod rplmilh lhe en-IlMI -urIm le
To pcJ 11lQl oacnrion ecn plodcd 1 f shyampcm dculqhouoc wllen Ilrock kad dcpooiled pIa oupIy 10 fCIIlt lier The iIIlon Theft iooLotcd doo= m~ DNA poI lT poIr
Copytlplt llln $dfUIc ~l F
-1 1 ~ Ile for 11 IN lu 101 oIlUJIlly 0Uf0m2I
cd rat ~b-m rIOCDC of PCll ho roploced dw T~
r-gtln- idI 1ftlt pol Th cnqmc ioulMed Icircfltlm die hypcnhlaquoshymolloiIf lroct (degflmi lircWIj Itlt -1 100 C
Adom-bc commcnioJ ~ fi linrtIcy w ~ allN C(~IR procMood fnIm tch CydoMlrxtrino ~ 10 lfabiliK talCultoIIo io frlnlthl In P doc ll4lab 01 mrdishyciDn br tIc body and hl -_ shy
odon onalitinet
cow an
_nr __Iho -lIr-shybodl - oI_mm drpaC(w JI F) a 0- Mlf lM oanhampco AoIlIIl1oftd __ cl dw Nnic lOlo Mshy1fUio pl wly dor for sshyp )r- plloshyn likc rhe tlorlfIf PPG Iik oh - oi MlthroplWoo (ltDld Iowcn~
Ju- T Sulqo ud Uuml -
~t=~ obioJ=-me lfUlI AntllClic bgtshyocean bullbullIf lhaI ffllllino froun for mudl cl yult TbrocCCOlll1WllIcirctico lashydudo uumldilcrilt karyabIJ alpe od dlofOllIS Il wdI Il bull ftlircy of borio 0 bolttnillbUMmiddot 1br Sulqo 1fOIIP oJ-p___dnmiddot io plinu ltpfe5ltnlgtti 01 PJIChro pIiIo oprisul prnnue lor -m io C od io fiumlogtdI -puashybom Il dtpeaCfOO kir uproclucocircoL CIl kbc OfF bull Iflllal fO im lUcircoawm who nccrl CDIJIbI dool rfriImiddot lIfQlltonar-- faocl __ 1-- produca uquifc w _~ ~
aukm oIlnampn_lhidl po
Il ~ ~11JtCl 01 Imiddotwbullbull tclry WPB A- exvemopQiln DO
liF - _ J ~ Aa bull 1 rio tJ __0 middotd ---a ~œbull Rr- -- ~ iwc S ncILIioiot __ -M
st _ca
w IId - tmiddot II _ = ~ loItroiNunli s Uoi ~
Il -u_)_~IcltoIc_
middotlJlllshy_ jIftIiNo woooW _--
F- Jt-o 1 e--tIodl _ 01 DN- J IU) ~ a - am_ _ az AI _ J - shy ro- __bull __ 01 cu bull 5 -~ -b _ nr ~ bull DNA 4a ad_
f T_~iuml+
bull fi n ldi-l- po- ltuhm 01 na rn u -If _ IR Iookont fol M ~
wli __
t Pt ho
11 7lltooblc
~DNA _~ 1 1 11_ doc 1 01 _ 1
loouumlo bull
-0 tz -d i1ioolI ~
-m t~iI~ 1 Icirc1idoou fi It_ bull dG) 100 = _alf
1CtdiIy aiochal_ Md ~ ~d r~~ ~ docl ---
bull i ~ J a 1ctW wlioltDfd Olt Il ad lLCiicy if ole 1 _hen 10 otl hnicoJ_ _ ~ mpoMlWo kir cho_ Doriry _g ha lmiddot_ _ doc LU lied rift iD oloocr M JUIcircOI doshy
bull ot in S
~bull--r CAO S 11lq dot DNA rr-~ _ -001 -wl n-n exshy_ao bullrn-_ __ DNA -1 u~
--li bull - shy -a_ _ li COlI i _
__ rilll _ shy IlIIHwI
Mt
S - -
~1997k~IIt_ -
~ altcNCfIn_~lt-flIlhIt_b1IliI ~(Mlbl_lollhe _1) IIH ioMlt ~ II aphlic OIll9flMII$lIM 9 _ Mon fA lIMue nNNl9tfle$ ~
_ - 1IIri Insighu lnta _ 1Ulttgt 1Ifo- pl 10laquo_1 JqJiIo IboocWrioL d cd ~ œnoIn ail _ -anboonollclf ~IDfODC SdiwIIlIU OW ID n IN _ bIrbo8OislJdbdtcI~9In__bullbull 0I1O
u ID _ Gmn bIutpmu ifonI _ PIfbm -11lt1 induumllt __rltlIldampul ail he-N
- 1
i 1 1 1
1 hdp tIIZJGlel from onlimrp rnicngtba inm anilicial U1twfId
DiIrofflY cl~OC- 0jlfkW MImiddotmiddot br_de 1 i aI au_dM _ pobiluumla Y for lIeOI cuyme lU pin ~=pta-itomair ilI lia~ 10 kttp down Ihc (Otef 0 pt(gtshy
dacrion m~~~tIw -1 xm_r= prodllCilll tamporr will rdUbly mm al PfOI Tho dtfkpllilaquo of ~ ufKlUriaa trdIniquco and thc le-
pm PfOIiUI Degtoota 01 he wshyet ~ known dirtard cYltgtlushy
OIlOl froaI
bon k Of 1 rudom nashyna the ~~al-
~ llW acnerthoo do of diffnm of the enzynw T1wn Ihey 0ClfII0l1hc shylU hnhee of lhe bullbullriItioGo pintd topod-b futurlt- Th Lui IUllteV il aloo Wd 10 he PdioonLon co ncn Ed -pt bull -loI 10 u bull 6ItmmT br doc liahlbtlb he lriod ~ ilable m bombou 10 oiIIr ttado nd dlott br_I WOIked bat
iCI far _ ntnlOltf1OtO in shyaM lime olœrod choir iampioal Mm n raQoltlaJ dollp NId Edooniaa ppruiOCha prumi la
halllaquo emlII(Iamp)lIl Tbey aloo
Loi
_____
-ER
Extremophiles as a source for novel enzymes Bertus van den BlXg
M_ lObe_ shy on bull _
bo_~ __ _ ft _--lI)r_oI_pH TI-__ _0 No IIogtOIIbool lnl
1Ol __---shy~ 00 br l-lq lodulriIoI dc d fOI bioatayJ do lM COI _ cooodiltions id obIlt cfllwto ~ Nu -od co lh ~ rOI _h uyma cmoaI arpalc tloaio bIcircolIc31alyI emn lM boette cheinl pl oohilth con kod 10 _ cl5acircelo pnodian 01 10 gttltragtIumlODltIgtltB (cr bull tt iffNIrtlltlllll nkn Il ~ ~ilC Ibc ampa do ID do _ dun JOOO dlll_ euyma lM oori6ed or thcfCl dleir-~ iDdtauiol pIi_ he pracA UZ)1IM bon niU _ 1Iccediloe bull de A0a_ dl iam foct _y Iob euyma lN _ wilttltond ml ~
-Ji As rauk dM lt~ iu almia Ole ~ i~_C1I famp_ hot igt pcoc dcugrave oudo zac-phila gtIe _ cl ~1U 1~J~
Eure QOnd fCfc 10 pII)l coIre le _pc Je ndirlti_ bult ID _
_ u1gt Ii~ad pH (rhI Il loi vt doc couemovtoilco h bec idltn~d Olle bclonJ 0 1 01 Ile AIclI bull _bullbull bullbullny nunoophilc r dgtc ria ond uy roc i bullbull o Illy ItIcntlllcd od dlancuriud
TIoc _ion ilco C090ble rviY uncla no eootchfoollt __lion1 shy_nu h Icd _ doc _nies Mw _ becn Yod roc_ oondm- 1 d bull -idaob6c r do t do hbullbull hcgtIotood d IIlncucircofldy cboruotiocd r- lU pH bull HP _ lIcircIIn
1 ~ l aDd diocua 0CCCm pl bull shydlo d-IJ iIoNoliobullbullnd ~ 01 ~ r _pt-_shyAi illu cd ln Tobie 1 lM el licol COllall numiddot lcr widc iEry d~ middot diliom Iugravech pnilm odoptcd nc b Iyo i_ m- doc =p cauld bclBmiddot
IIIlpliablc ibltty dio-ne fToble 1) F do dep cL po)mcn dli _ bull _ r- dobullbull _ aetiw bull and - 10 ltiIlt ~nfCIl ohe prcfcrrcd Us- the _ lb ooIwl Ugtd __tIy oIIc litlt libiliq tbc boltnf p_ AltanoIJ ir lgtftC necbullbull pcltshyfoc RCf_peooilk odIl1cUcircltXI 0( ltOnfIOOlnd lof tbc lDdlais cL bull phomuautioaIly le pouduco Ofpnilt oohc IJ diIf nl ~i ply bull the 1Hoo1you AI II Icirci rclthcc --- ocorilty tIZflH m- loIcgtpIlilif le OIIOIld bc _ ibIc clioicc r pptaiDn i__-dia 1~5I n Ji ~ ofn i_mc lOI ~ di ltIfICplile c dopltcd off yaciriof fIlin rOf 0 ~riuy 01 oppli(otiofto
_ hM TIocpliilt CJIrcmophilco ~ cd _ ne rion kul umophlllc ptOIlt_ li poIy-dqndil lt110) IUCh n dlul h bull d yI ~Vll rotlCl lb IAlI Inod il _10 IThIc 1~ Tho fCO-o 1)1
Otobk nd oeil ol ~cd PfM- obtmiddot A icnd nou he oolubilify 01 noy ion laquogtngtpOfInll in penk ltr po)merlc oubouo bull liancly cd MOfco~ dobullbullioIr 01 11oft ludinl 0 undctifld 00000pliulOf 1 uh ed hipMr
Tbo 01 fta oIlhampl1llOpbik ee Irtrgtctltd mlEh lttenu- Sccnol duoc-dimcnMoMl _fU~ ho lcIcn lOigtlIltI oAd comd 11 m_ of coophilie tefJWtt wim Illagravemo ellOCOcircdoriooc ltIre - tfylll Ile-- hi Irr~ ln 3 ltraquomfgtbc c -ud) 10 ~iIic ud hypudlumoptlJic 0CfUcirclC hftœJlUhranofcntco ewnn-d Omiddot1r 53 __phil ~ 1(] sncshyr olipmclM lAd 1ocJ fIgtCIIkIrIIr pplicd III idcatifT ltIre diffete_ 1 n ingttoloul - 1iIy f dl eazr- doM i _ a-doolcd Iioy ocM-gtocd Irt 0 11 -1 ~e ehofIC incrtoscd prodn ~yd lCgtlT _ ~lllaquo_ or upoocd middotdgteobiIc
id roamp_-J7 foom poych_ bccamc -- criItI PIIlico pudJ beauoc or 0flIGi cff III de=-~ p For ~ doc il CIl inctClhllC deoifc 10 IPPIy br phitoc ~ dacn Wh ovdI ~ i b r-w III dcoe Ioullliry oppu-iono d _ _ pc ltd Iowa lCODpcru l oudIo poogtshy
___ 1rnlPIliIiO P P _) ltH li__ tOC pel -J L T1lc pulp ond pope indulltry il IIM inrated 100 poiylllC-dclflllilll emct 110 lICrift Iow PC Sc1 ood PnxaRnl opplkMId ohe _ndl r e YOilbil PC ermyma
AltMOCri u of ny ens) r psyd plile 10 he ltl fil hido nllltie Ioi ft1
II lObOliry Il hpu wlIich cu lia pon CIlploJncd Irt tllc iumlc-d IC1Ocirc1lUumli r die le illl _1Opli1ilt ond Ill plilic nq-_ Th plO_ of cazyooa Iow oc_ eojeco of bull ] ia 11l-15~ Il h hea --cd hop iwJ lcsitlilioT il illed widl eued biJic d 1 det botmiddot ca bctItecn bUC Ind tinc bull Icircoldcltd ofal ohoc Neeukclc cre i n i_llia ber or r ~ N bullbull prolan e~
Meri _c1lebullbull-bullbull lilooo lb no ruIU ~ Icircol blI or an bc dilfc nl indop ~dy 116-111bull 11001 middotmiddotie of pn kd prope ar encrma r prrchroplralad cio Ipplicorgtou oon bc (ouad III 0 11Cicwo lm- ll~_Hlopliko con uibullbull ill hpcJieo lrobilO bf Illel obiJ to lnlO _le boJ__ _am 1cII u mdium Of POUUnl elllaridc (NoCl or Ka) up _n Ill ole _le m the ctniftln__menL AI 1 poole r- holophila M ltOpe illl ry hip oak _nnriooo le4- KCl ___
or ~ 1 Sa n_ ohS nlUJ~
The 1Ul- hne odopd bullbulln M 1 PO wc by ocqinnl eloocirc pt bct ar ocircI dlrpd ocld -ofilaa IO~
pfClipiIOUgraveOiL CoaUy le fi iP ~ lOI __bull dtc ilioy lIilic e_ ca nmiddot plllf ~middotil kllimn oppIlo 1ic r~~
H_ ptQpcny toc 1Ou n of by -
_ _ bullbull _ 115
1~h~ heur- bull _ _ bull 1Sl-Fr r _ c_ ___ Il _ r cmiddot bull __ -( d = t I~
MeIiPt1=bullbull eIEtel - _ pH _w mol lIrM ~ _ __ r c dot lI_c_ dclter _ Irillile pl ~ - Ilftd Iblt shyilic -pa io dieu oIMliry _1IW11 toI lllc lIIIluar-~ e-pn _ j lU al _
poltnfI ligt bull duo __ r cw-1I1 CI wtad han (ru- lit pli cn_ in he a el pt lia oncI llco le 1
_yIooa U- odoclt na - _ pH locII ____ _ ~ 110iallro C bullbulllbliph a-rir ~ r dln aiIilty bull 1 t c S _ - -rot __ j c Il PCa-r iIaIoC _ _ bullbullbullbull S _
t shy a IoococN_ _ 1-1 loi _ _ H middotrmiddotfH_ ___ bull Il llOl
OB ~ ncccdiloooM d icircnd tIoe 1iNI el du -u 1 Id lie -n dio OCII
- -shy
_- middot - n bull i lgtgtMf 1Ul d - _ _ - --shy X el
OlhIrtibam1 Ai xo_ _ _ 1 _1-
110
u p _ a _ _ pIOO -a_
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_ ai dy bcJIC t -
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b _ bplr lbull
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br 1 obbullbull dfic rmn~ pnxcd (c fedmiddotIlIICh laquoII cclon 0lt nnllllUll cul ) 1amp tdcli opcucircol biolteoclOlt II bullbull ampu-lih rUl CI d ohu Ilw bc cd r pk tpecific ioIIroio Woo- 1000II 00 lie _Iopcd in rhbullbullulioo ofccloalopbila r pad orpolyJJum d (VGII~IOr opcc1lic req inshy W oIic b bol ore Iorcd he IoiJlb ra dl IIK dIbull-rillic 01 on i_n f lucircdIo 01lfClIIIi ioolcd 1IIdI dl _l~jl
Gene do-d orproducllon At p the fc _ pod n II101Y fol lMOIlpIlocirclu _ -ocirc6cltcd rd pmshyltcdn nurdon mil oI kinamp 1 Wy1aoOUcirctlbler rhnc laquoCII IIlO bc deyckI9cd ThbullbullI_inl rid bull i_ r lIIgtlidinJ amplt in pa1Ic ~ CI le n UMllve olmOlive lIlodlOltl- A jIlr ion or h carymbullbull ILo h r dl y 1 indalolppl _ 0 _ don Edma roJi uptaUgraveltgta -rou- ( T blc Z bull 1ll1I+lB
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---_ Md eka o ~ - cJiM ond Hu The objccti of ~ uld le po i id in Iectiloamp lie Ic buM YIII P pl ir dlbullbullnq- cd ampIlltOIyl-oon farltiolopol ocri dl Icotiod of a bo ri1 Il oold bol votdcd wh ro dl poodllttion or u llllla cn_ 0 IKioI bulldo Btit bull likcty 10 le Ie A dJlWIJoclt or- ampnIIlu-loucd - bullbullIe dri~11 hb Iel r odn p_ _ dl Il lIioll or ludo d ion 01 toshyrocou cene prod of i RelaquoI1 dshylu plOltC -dcJiciulbullbull lnrJu YC _ d bco prod1an leYeh or _c 1lIl~
s-ol 01 u _1lIncd buw ppliable ouroc 01 __ ribnlriu ln __~ a bc 01 dloic dI_ plin I+IwoI9) s--nhc~ i ufM rkk ofOcircOldOllioolmiddotbody ~ M do bullbull E Il - plOt pan biiugrave Ite Id1oo 1tc 1idd
nu ilobilil-r vlplOd 11I1 -po Iloo _d _ nd raiIooiq ~ propenico _y Il fNllCl CIcircCriItc d _dorrl ln fVIICUw diccud bull __ Iurion i ~a plt~1I elliccediliucy 01 oucll)tf Oftc 100 1icircIlnria (10_10 nrion) MW bc d nriollQ dcoftd lI5lUllod rc )
Condu_ne and penpecthon Eltnm uYC _- 101 in indQono pngtlaquolt ludiampriltltu~ cIIc1 nd ial opplia W _ ptOd 0 60 ftooooI rlgtc IIddlriool ploi oioa efltn-- TW lboI 100 ond te m iblc i odily bullbullpndi Il ba arcd tho I bu lOS lIf lin 1 1 bull tldncd CClI will bc ltul 1c nd _ rhe ocirc or _ upreooion dlllllIlu (cc dcTclopfncn of lt11 ud im _ Iopo kor _m) _~I 10- up 01 roltlt dil Ir i n_ lgtlc 10 CC op lib egrave f lhe diWIK _ Ir h llbl orn ned w11 r ond 1laquo11 tlc_ rion IIlc n œy will bol dl d la 00- _errraoom le u i ye b WOegrave p fiosoeOf e opecoftc WO 11y lricdly ocu deyclo 01 pooteOcircII Ocircllamp lItd dicd cYOIulioraquo cil bullbull oilI 10 iIl unitbullbull am Ocircl biocotolpic ilS wicll -ill iolaquoco tllo pp_ 01 bull f_ uraaopbil
PlINlSI-lLlG fNVIRONMINT5 1ft bull ~ n ht-r-d 1000lt_ dtpicted
H middot ~middot~ phi III -Dl - ondiod
ol utraIIogtpIugraveln l1Mml gtoJ ur tUdilr Iollmlpmmiddot mres ~a mll 4$ cnw II Il dovltn Flvmhm and ltli rhrm t=d hl~ ro lomIp--3- 10 dosr- C (116 q FI Sltwnr h~ ph ~- lhm Il 11_ 100bull ln Iwo 100 ltleyeltr CIll drrHt f 1Iw hagrave-l POO nl ~ ~bulleo ri III pgtrioœ prdm]Inlttv hamshyrU puw fau in lUffJ -m 1J and lt10 ltltlIren C (77 -J 104 ~ Ft r~ no mllulJ 1gt pLul h~C hmI foonJ 0 NImiddot 3~ rltrm-n ~hobullbullbullbu sa LIemiddot c (lU sr- flbullbullnd _ bul fi d~-I Cln roIn-
bull rm 1lfIampU m remrrnNlllJher dwI bout 6(1 dqren C (HO ~awenI penlWD fi 10 6(1 LIepeo C hu-n a- b bur plusmn 10 lwmtI in aream hul-wftlt lino d ~ _boat JO JU n D Idlt motltd ampc l1nIlt-a of IV _~- op 1__ ml m fPlaquogt_ ltJuriaC bull ~-ltcna -=rob1 61on hot ~ oi NoaonoI Pul W~
no __p iDuDd 1Iw _
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CopyrlaIll lm Sdonllllt uMrilt
0 sJ(uIuln tUltinu IW pllfiaRy bullbull ptmNfft -1IiP _ as ~ c n ~ 1 anI con ht pr-ricircn boiIinf shy
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10- mon +0 saae al hy pmhomaogtphib lu hem -wd -r~O s-n cUshy~ mUao-yal~
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bull
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Noe Too Hoc III H-IIo
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_pcnrr CUumlIHL 1111 il shydolt _ft o1dlouch IcircI _ _
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In lC1 - DNA poIltUJIOcirclIlur1onipptt01 DNA prodllQ 0__ p p)l Tho _011 roiaor 10 lIecyccd Ioorwcco lowarwl _W1IaoMshylion iol -l ~ohcpoty-- _ Ire- aobct tIlIf bullbulln ROC l Licnd 00 0f01lltd kmiddot
lbc Iwx plll 01 plcnd~bull Tlaquohnitwu hod rplmilh lhe en-IlMI -urIm le
To pcJ 11lQl oacnrion ecn plodcd 1 f shyampcm dculqhouoc wllen Ilrock kad dcpooiled pIa oupIy 10 fCIIlt lier The iIIlon Theft iooLotcd doo= m~ DNA poI lT poIr
Copytlplt llln $dfUIc ~l F
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cd rat ~b-m rIOCDC of PCll ho roploced dw T~
r-gtln- idI 1ftlt pol Th cnqmc ioulMed Icircfltlm die hypcnhlaquoshymolloiIf lroct (degflmi lircWIj Itlt -1 100 C
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Ju- T Sulqo ud Uuml -
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aukm oIlnampn_lhidl po
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st _ca
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f T_~iuml+
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loouumlo bull
-0 tz -d i1ioolI ~
-m t~iI~ 1 Icirc1idoou fi It_ bull dG) 100 = _alf
1CtdiIy aiochal_ Md ~ ~d r~~ ~ docl ---
bull i ~ J a 1ctW wlioltDfd Olt Il ad lLCiicy if ole 1 _hen 10 otl hnicoJ_ _ ~ mpoMlWo kir cho_ Doriry _g ha lmiddot_ _ doc LU lied rift iD oloocr M JUIcircOI doshy
bull ot in S
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--li bull - shy -a_ _ li COlI i _
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_ - 1IIri Insighu lnta _ 1Ulttgt 1Ifo- pl 10laquo_1 JqJiIo IboocWrioL d cd ~ œnoIn ail _ -anboonollclf ~IDfODC SdiwIIlIU OW ID n IN _ bIrbo8OislJdbdtcI~9In__bullbull 0I1O
u ID _ Gmn bIutpmu ifonI _ PIfbm -11lt1 induumllt __rltlIldampul ail he-N
- 1
i 1 1 1
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DiIrofflY cl~OC- 0jlfkW MImiddotmiddot br_de 1 i aI au_dM _ pobiluumla Y for lIeOI cuyme lU pin ~=pta-itomair ilI lia~ 10 kttp down Ihc (Otef 0 pt(gtshy
dacrion m~~~tIw -1 xm_r= prodllCilll tamporr will rdUbly mm al PfOI Tho dtfkpllilaquo of ~ ufKlUriaa trdIniquco and thc le-
pm PfOIiUI Degtoota 01 he wshyet ~ known dirtard cYltgtlushy
OIlOl froaI
bon k Of 1 rudom nashyna the ~~al-
~ llW acnerthoo do of diffnm of the enzynw T1wn Ihey 0ClfII0l1hc shylU hnhee of lhe bullbullriItioGo pintd topod-b futurlt- Th Lui IUllteV il aloo Wd 10 he PdioonLon co ncn Ed -pt bull -loI 10 u bull 6ItmmT br doc liahlbtlb he lriod ~ ilable m bombou 10 oiIIr ttado nd dlott br_I WOIked bat
iCI far _ ntnlOltf1OtO in shyaM lime olœrod choir iampioal Mm n raQoltlaJ dollp NId Edooniaa ppruiOCha prumi la
halllaquo emlII(Iamp)lIl Tbey aloo
Loi
_____
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Extremophiles as a source for novel enzymes Bertus van den BlXg
M_ lObe_ shy on bull _
bo_~ __ _ ft _--lI)r_oI_pH TI-__ _0 No IIogtOIIbool lnl
1Ol __---shy~ 00 br l-lq lodulriIoI dc d fOI bioatayJ do lM COI _ cooodiltions id obIlt cfllwto ~ Nu -od co lh ~ rOI _h uyma cmoaI arpalc tloaio bIcircolIc31alyI emn lM boette cheinl pl oohilth con kod 10 _ cl5acircelo pnodian 01 10 gttltragtIumlODltIgtltB (cr bull tt iffNIrtlltlllll nkn Il ~ ~ilC Ibc ampa do ID do _ dun JOOO dlll_ euyma lM oori6ed or thcfCl dleir-~ iDdtauiol pIi_ he pracA UZ)1IM bon niU _ 1Iccediloe bull de A0a_ dl iam foct _y Iob euyma lN _ wilttltond ml ~
-Ji As rauk dM lt~ iu almia Ole ~ i~_C1I famp_ hot igt pcoc dcugrave oudo zac-phila gtIe _ cl ~1U 1~J~
Eure QOnd fCfc 10 pII)l coIre le _pc Je ndirlti_ bult ID _
_ u1gt Ii~ad pH (rhI Il loi vt doc couemovtoilco h bec idltn~d Olle bclonJ 0 1 01 Ile AIclI bull _bullbull bullbullny nunoophilc r dgtc ria ond uy roc i bullbull o Illy ItIcntlllcd od dlancuriud
TIoc _ion ilco C090ble rviY uncla no eootchfoollt __lion1 shy_nu h Icd _ doc _nies Mw _ becn Yod roc_ oondm- 1 d bull -idaob6c r do t do hbullbull hcgtIotood d IIlncucircofldy cboruotiocd r- lU pH bull HP _ lIcircIIn
1 ~ l aDd diocua 0CCCm pl bull shydlo d-IJ iIoNoliobullbullnd ~ 01 ~ r _pt-_shyAi illu cd ln Tobie 1 lM el licol COllall numiddot lcr widc iEry d~ middot diliom Iugravech pnilm odoptcd nc b Iyo i_ m- doc =p cauld bclBmiddot
IIIlpliablc ibltty dio-ne fToble 1) F do dep cL po)mcn dli _ bull _ r- dobullbull _ aetiw bull and - 10 ltiIlt ~nfCIl ohe prcfcrrcd Us- the _ lb ooIwl Ugtd __tIy oIIc litlt libiliq tbc boltnf p_ AltanoIJ ir lgtftC necbullbull pcltshyfoc RCf_peooilk odIl1cUcircltXI 0( ltOnfIOOlnd lof tbc lDdlais cL bull phomuautioaIly le pouduco Ofpnilt oohc IJ diIf nl ~i ply bull the 1Hoo1you AI II Icirci rclthcc --- ocorilty tIZflH m- loIcgtpIlilif le OIIOIld bc _ ibIc clioicc r pptaiDn i__-dia 1~5I n Ji ~ ofn i_mc lOI ~ di ltIfICplile c dopltcd off yaciriof fIlin rOf 0 ~riuy 01 oppli(otiofto
_ hM TIocpliilt CJIrcmophilco ~ cd _ ne rion kul umophlllc ptOIlt_ li poIy-dqndil lt110) IUCh n dlul h bull d yI ~Vll rotlCl lb IAlI Inod il _10 IThIc 1~ Tho fCO-o 1)1
Otobk nd oeil ol ~cd PfM- obtmiddot A icnd nou he oolubilify 01 noy ion laquogtngtpOfInll in penk ltr po)merlc oubouo bull liancly cd MOfco~ dobullbullioIr 01 11oft ludinl 0 undctifld 00000pliulOf 1 uh ed hipMr
Tbo 01 fta oIlhampl1llOpbik ee Irtrgtctltd mlEh lttenu- Sccnol duoc-dimcnMoMl _fU~ ho lcIcn lOigtlIltI oAd comd 11 m_ of coophilie tefJWtt wim Illagravemo ellOCOcircdoriooc ltIre - tfylll Ile-- hi Irr~ ln 3 ltraquomfgtbc c -ud) 10 ~iIic ud hypudlumoptlJic 0CfUcirclC hftœJlUhranofcntco ewnn-d Omiddot1r 53 __phil ~ 1(] sncshyr olipmclM lAd 1ocJ fIgtCIIkIrIIr pplicd III idcatifT ltIre diffete_ 1 n ingttoloul - 1iIy f dl eazr- doM i _ a-doolcd Iioy ocM-gtocd Irt 0 11 -1 ~e ehofIC incrtoscd prodn ~yd lCgtlT _ ~lllaquo_ or upoocd middotdgteobiIc
id roamp_-J7 foom poych_ bccamc -- criItI PIIlico pudJ beauoc or 0flIGi cff III de=-~ p For ~ doc il CIl inctClhllC deoifc 10 IPPIy br phitoc ~ dacn Wh ovdI ~ i b r-w III dcoe Ioullliry oppu-iono d _ _ pc ltd Iowa lCODpcru l oudIo poogtshy
___ 1rnlPIliIiO P P _) ltH li__ tOC pel -J L T1lc pulp ond pope indulltry il IIM inrated 100 poiylllC-dclflllilll emct 110 lICrift Iow PC Sc1 ood PnxaRnl opplkMId ohe _ndl r e YOilbil PC ermyma
AltMOCri u of ny ens) r psyd plile 10 he ltl fil hido nllltie Ioi ft1
II lObOliry Il hpu wlIich cu lia pon CIlploJncd Irt tllc iumlc-d IC1Ocirc1lUumli r die le illl _1Opli1ilt ond Ill plilic nq-_ Th plO_ of cazyooa Iow oc_ eojeco of bull ] ia 11l-15~ Il h hea --cd hop iwJ lcsitlilioT il illed widl eued biJic d 1 det botmiddot ca bctItecn bUC Ind tinc bull Icircoldcltd ofal ohoc Neeukclc cre i n i_llia ber or r ~ N bullbull prolan e~
Meri _c1lebullbull-bullbull lilooo lb no ruIU ~ Icircol blI or an bc dilfc nl indop ~dy 116-111bull 11001 middotmiddotie of pn kd prope ar encrma r prrchroplralad cio Ipplicorgtou oon bc (ouad III 0 11Cicwo lm- ll~_Hlopliko con uibullbull ill hpcJieo lrobilO bf Illel obiJ to lnlO _le boJ__ _am 1cII u mdium Of POUUnl elllaridc (NoCl or Ka) up _n Ill ole _le m the ctniftln__menL AI 1 poole r- holophila M ltOpe illl ry hip oak _nnriooo le4- KCl ___
or ~ 1 Sa n_ ohS nlUJ~
The 1Ul- hne odopd bullbulln M 1 PO wc by ocqinnl eloocirc pt bct ar ocircI dlrpd ocld -ofilaa IO~
pfClipiIOUgraveOiL CoaUy le fi iP ~ lOI __bull dtc ilioy lIilic e_ ca nmiddot plllf ~middotil kllimn oppIlo 1ic r~~
H_ ptQpcny toc 1Ou n of by -
_ _ bullbull _ 115
1~h~ heur- bull _ _ bull 1Sl-Fr r _ c_ ___ Il _ r cmiddot bull __ -( d = t I~
MeIiPt1=bullbull eIEtel - _ pH _w mol lIrM ~ _ __ r c dot lI_c_ dclter _ Irillile pl ~ - Ilftd Iblt shyilic -pa io dieu oIMliry _1IW11 toI lllc lIIIluar-~ e-pn _ j lU al _
poltnfI ligt bull duo __ r cw-1I1 CI wtad han (ru- lit pli cn_ in he a el pt lia oncI llco le 1
_yIooa U- odoclt na - _ pH locII ____ _ ~ 110iallro C bullbulllbliph a-rir ~ r dln aiIilty bull 1 t c S _ - -rot __ j c Il PCa-r iIaIoC _ _ bullbullbullbull S _
t shy a IoococN_ _ 1-1 loi _ _ H middotrmiddotfH_ ___ bull Il llOl
OB ~ ncccdiloooM d icircnd tIoe 1iNI el du -u 1 Id lie -n dio OCII
- -shy
_- middot - n bull i lgtgtMf 1Ul d - _ _ - --shy X el
OlhIrtibam1 Ai xo_ _ _ 1 _1-
110
u p _ a _ _ pIOO -a_
t-S1lo) C ( b) t- T10c l 15 bull r- - nu ~ilco _ M No-shyrIldur bull _ bullbull dot lt1 0 el ~ il il n likcly dIIlt _ pa illoc will dolt PP- eI _ frual ud cuoclMflhiieo __MI
Exlr g~
TM ber 01 ee-co nilblc ~ 10 d Id bullbull rt Md iIeMi6lte ollOlacircll-l~cw-dybullbullbull 1co0l101__ r-AJnrcr Un cMifdt Ill IZ _ pd bullbull IJII dl f-ou =UIOI 1t jllr
_ ai dy bcJIC t -
o IrtiLtii ] bullbull _-_--- 15 bull fliTJ f bull 1 13IL _ bull SIKl shy(OUO) __ 1 ( r 1_ OUI _
t _ pnbk e 110 -lDNA-- bullbull JII 1 l _ _ m-doc bull - ou ~l~~_
r Ir -_ -doc __~ I
-Ic n _ bullbull iool no lllio _ ~ 110 dlc J S _ ~ ta U t n bullbullmiddot - 11 iIoc bull bull OH JI
b _ bplr lbull
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nu ilobilil-r vlplOd 11I1 -po Iloo _d _ nd raiIooiq ~ propenico _y Il fNllCl CIcircCriItc d _dorrl ln fVIICUw diccud bull __ Iurion i ~a plt~1I elliccediliucy 01 oucll)tf Oftc 100 1icircIlnria (10_10 nrion) MW bc d nriollQ dcoftd lI5lUllod rc )
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dacrion m~~~tIw -1 xm_r= prodllCilll tamporr will rdUbly mm al PfOI Tho dtfkpllilaquo of ~ ufKlUriaa trdIniquco and thc le-
pm PfOIiUI Degtoota 01 he wshyet ~ known dirtard cYltgtlushy
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Extremophiles as a source for novel enzymes Bertus van den BlXg
M_ lObe_ shy on bull _
bo_~ __ _ ft _--lI)r_oI_pH TI-__ _0 No IIogtOIIbool lnl
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Eure QOnd fCfc 10 pII)l coIre le _pc Je ndirlti_ bult ID _
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_ hM TIocpliilt CJIrcmophilco ~ cd _ ne rion kul umophlllc ptOIlt_ li poIy-dqndil lt110) IUCh n dlul h bull d yI ~Vll rotlCl lb IAlI Inod il _10 IThIc 1~ Tho fCO-o 1)1
Otobk nd oeil ol ~cd PfM- obtmiddot A icnd nou he oolubilify 01 noy ion laquogtngtpOfInll in penk ltr po)merlc oubouo bull liancly cd MOfco~ dobullbullioIr 01 11oft ludinl 0 undctifld 00000pliulOf 1 uh ed hipMr
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id roamp_-J7 foom poych_ bccamc -- criItI PIIlico pudJ beauoc or 0flIGi cff III de=-~ p For ~ doc il CIl inctClhllC deoifc 10 IPPIy br phitoc ~ dacn Wh ovdI ~ i b r-w III dcoe Ioullliry oppu-iono d _ _ pc ltd Iowa lCODpcru l oudIo poogtshy
___ 1rnlPIliIiO P P _) ltH li__ tOC pel -J L T1lc pulp ond pope indulltry il IIM inrated 100 poiylllC-dclflllilll emct 110 lICrift Iow PC Sc1 ood PnxaRnl opplkMId ohe _ndl r e YOilbil PC ermyma
AltMOCri u of ny ens) r psyd plile 10 he ltl fil hido nllltie Ioi ft1
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or ~ 1 Sa n_ ohS nlUJ~
The 1Ul- hne odopd bullbulln M 1 PO wc by ocqinnl eloocirc pt bct ar ocircI dlrpd ocld -ofilaa IO~
pfClipiIOUgraveOiL CoaUy le fi iP ~ lOI __bull dtc ilioy lIilic e_ ca nmiddot plllf ~middotil kllimn oppIlo 1ic r~~
H_ ptQpcny toc 1Ou n of by -
_ _ bullbull _ 115
1~h~ heur- bull _ _ bull 1Sl-Fr r _ c_ ___ Il _ r cmiddot bull __ -( d = t I~
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poltnfI ligt bull duo __ r cw-1I1 CI wtad han (ru- lit pli cn_ in he a el pt lia oncI llco le 1
_yIooa U- odoclt na - _ pH locII ____ _ ~ 110iallro C bullbulllbliph a-rir ~ r dln aiIilty bull 1 t c S _ - -rot __ j c Il PCa-r iIaIoC _ _ bullbullbullbull S _
t shy a IoococN_ _ 1-1 loi _ _ H middotrmiddotfH_ ___ bull Il llOl
OB ~ ncccdiloooM d icircnd tIoe 1iNI el du -u 1 Id lie -n dio OCII
- -shy
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110
u p _ a _ _ pIOO -a_
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Exlr g~
TM ber 01 ee-co nilblc ~ 10 d Id bullbull rt Md iIeMi6lte ollOlacircll-l~cw-dybullbullbull 1co0l101__ r-AJnrcr Un cMifdt Ill IZ _ pd bullbull IJII dl f-ou =UIOI 1t jllr
_ ai dy bcJIC t -
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t _ pnbk e 110 -lDNA-- bullbull JII 1 l _ _ m-doc bull - ou ~l~~_
r Ir -_ -doc __~ I
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b _ bplr lbull
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III 1 fIltIICIloirlly dIIl pood
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Productkln lJl MZ)1Mo1 fovm ~
rlaquoriotuJ 0lt0Yf IMIMI i oIDNA ltII bc very bdprul ra rd lJIla1ion fil _fui euym ll_ebullbull Ile idaI_ of polUUIllIy fulnynICS i Iy lino 09 To uIIIlI _ of lbocirc kMtIt idUlrifMd ca u 10 bc de bullniloblc fol ~ dl u vi b 1yUc ic1IIgtd Iicu_ -~ _ _pu lot the pnocI__ofCIU) ~ cm WJa r pIOdrioa or me biouIY Ile ~ d bJ 1amp biocDuo PIIdIoltdon of dle hile AJ_lIYely die CC eocodi do bionulyst n bc 1 d OcircII iable toool
Eltbbullbull1OPhIt blom_ ptOductIon Spococircolixd cqooip of~ bull Plaquo40i 0 owla fficic_ oIopecific bionllJyst dl1y f Ul~lIilolnddiioniIDrmiddotmod f lloA _ ril 1 bc debullbull1opnr ond 0 ho bc Iddruoed inM Iou- or _ pIoocircl Hl-~ lM- J)looJ ioIl ft bc -cd OcircM lgty 09iI of b alipoougrave Of
br 1 obbullbull dfic rmn~ pnxcd (c fedmiddotIlIICh laquoII cclon 0lt nnllllUll cul ) 1amp tdcli opcucircol biolteoclOlt II bullbull ampu-lih rUl CI d ohu Ilw bc cd r pk tpecific ioIIroio Woo- 1000II 00 lie _Iopcd in rhbullbullulioo ofccloalopbila r pad orpolyJJum d (VGII~IOr opcc1lic req inshy W oIic b bol ore Iorcd he IoiJlb ra dl IIK dIbull-rillic 01 on i_n f lucircdIo 01lfClIIIi ioolcd 1IIdI dl _l~jl
Gene do-d orproducllon At p the fc _ pod n II101Y fol lMOIlpIlocirclu _ -ocirc6cltcd rd pmshyltcdn nurdon mil oI kinamp 1 Wy1aoOUcirctlbler rhnc laquoCII IIlO bc deyckI9cd ThbullbullI_inl rid bull i_ r lIIgtlidinJ amplt in pa1Ic ~ CI le n UMllve olmOlive lIlodlOltl- A jIlr ion or h carymbullbull ILo h r dl y 1 indalolppl _ 0 _ don Edma roJi uptaUgraveltgta -rou- ( T blc Z bull 1ll1I+lB
Gribullbullool for 1 (01i1 ete Wl 11 docw opcd nlt y p1aid vctt1HI iDd illk lue Pp ocircbic 1 c p dllCUcircMl 0( ilegrave nq rili d dlc ctllodaoaN01_ E _ bullbullbull folio ta Il~1~ H dl middotenmiddotk_n pbc__ 01 im_-locidy r_lion by 0 middot d p_oocirc i t nli 1lt1 fOlCC h ra oI i iflaUil_Ir M 11 _bco of ugravew nllbk ncludil bonc101 11 111 dI ~a ~ ~h
---_ Md eka o ~ - cJiM ond Hu The objccti of ~ uld le po i id in Iectiloamp lie Ic buM YIII P pl ir dlbullbullnq- cd ampIlltOIyl-oon farltiolopol ocri dl Icotiod of a bo ri1 Il oold bol votdcd wh ro dl poodllttion or u llllla cn_ 0 IKioI bulldo Btit bull likcty 10 le Ie A dJlWIJoclt or- ampnIIlu-loucd - bullbullIe dri~11 hb Iel r odn p_ _ dl Il lIioll or ludo d ion 01 toshyrocou cene prod of i RelaquoI1 dshylu plOltC -dcJiciulbullbull lnrJu YC _ d bco prod1an leYeh or _c 1lIl~
s-ol 01 u _1lIncd buw ppliable ouroc 01 __ ribnlriu ln __~ a bc 01 dloic dI_ plin I+IwoI9) s--nhc~ i ufM rkk ofOcircOldOllioolmiddotbody ~ M do bullbull E Il - plOt pan biiugrave Ite Id1oo 1tc 1idd
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Extremophiles as a source for novel enzymes Bertus van den BlXg
M_ lObe_ shy on bull _
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pm PfOIiUI Degtoota 01 he wshyet ~ known dirtard cYltgtlushy
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bon k Of 1 rudom nashyna the ~~al-
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Loi
_____
-ER
Extremophiles as a source for novel enzymes Bertus van den BlXg
M_ lObe_ shy on bull _
bo_~ __ _ ft _--lI)r_oI_pH TI-__ _0 No IIogtOIIbool lnl
1Ol __---shy~ 00 br l-lq lodulriIoI dc d fOI bioatayJ do lM COI _ cooodiltions id obIlt cfllwto ~ Nu -od co lh ~ rOI _h uyma cmoaI arpalc tloaio bIcircolIc31alyI emn lM boette cheinl pl oohilth con kod 10 _ cl5acircelo pnodian 01 10 gttltragtIumlODltIgtltB (cr bull tt iffNIrtlltlllll nkn Il ~ ~ilC Ibc ampa do ID do _ dun JOOO dlll_ euyma lM oori6ed or thcfCl dleir-~ iDdtauiol pIi_ he pracA UZ)1IM bon niU _ 1Iccediloe bull de A0a_ dl iam foct _y Iob euyma lN _ wilttltond ml ~
-Ji As rauk dM lt~ iu almia Ole ~ i~_C1I famp_ hot igt pcoc dcugrave oudo zac-phila gtIe _ cl ~1U 1~J~
Eure QOnd fCfc 10 pII)l coIre le _pc Je ndirlti_ bult ID _
_ u1gt Ii~ad pH (rhI Il loi vt doc couemovtoilco h bec idltn~d Olle bclonJ 0 1 01 Ile AIclI bull _bullbull bullbullny nunoophilc r dgtc ria ond uy roc i bullbull o Illy ItIcntlllcd od dlancuriud
TIoc _ion ilco C090ble rviY uncla no eootchfoollt __lion1 shy_nu h Icd _ doc _nies Mw _ becn Yod roc_ oondm- 1 d bull -idaob6c r do t do hbullbull hcgtIotood d IIlncucircofldy cboruotiocd r- lU pH bull HP _ lIcircIIn
1 ~ l aDd diocua 0CCCm pl bull shydlo d-IJ iIoNoliobullbullnd ~ 01 ~ r _pt-_shyAi illu cd ln Tobie 1 lM el licol COllall numiddot lcr widc iEry d~ middot diliom Iugravech pnilm odoptcd nc b Iyo i_ m- doc =p cauld bclBmiddot
IIIlpliablc ibltty dio-ne fToble 1) F do dep cL po)mcn dli _ bull _ r- dobullbull _ aetiw bull and - 10 ltiIlt ~nfCIl ohe prcfcrrcd Us- the _ lb ooIwl Ugtd __tIy oIIc litlt libiliq tbc boltnf p_ AltanoIJ ir lgtftC necbullbull pcltshyfoc RCf_peooilk odIl1cUcircltXI 0( ltOnfIOOlnd lof tbc lDdlais cL bull phomuautioaIly le pouduco Ofpnilt oohc IJ diIf nl ~i ply bull the 1Hoo1you AI II Icirci rclthcc --- ocorilty tIZflH m- loIcgtpIlilif le OIIOIld bc _ ibIc clioicc r pptaiDn i__-dia 1~5I n Ji ~ ofn i_mc lOI ~ di ltIfICplile c dopltcd off yaciriof fIlin rOf 0 ~riuy 01 oppli(otiofto
_ hM TIocpliilt CJIrcmophilco ~ cd _ ne rion kul umophlllc ptOIlt_ li poIy-dqndil lt110) IUCh n dlul h bull d yI ~Vll rotlCl lb IAlI Inod il _10 IThIc 1~ Tho fCO-o 1)1
Otobk nd oeil ol ~cd PfM- obtmiddot A icnd nou he oolubilify 01 noy ion laquogtngtpOfInll in penk ltr po)merlc oubouo bull liancly cd MOfco~ dobullbullioIr 01 11oft ludinl 0 undctifld 00000pliulOf 1 uh ed hipMr
Tbo 01 fta oIlhampl1llOpbik ee Irtrgtctltd mlEh lttenu- Sccnol duoc-dimcnMoMl _fU~ ho lcIcn lOigtlIltI oAd comd 11 m_ of coophilie tefJWtt wim Illagravemo ellOCOcircdoriooc ltIre - tfylll Ile-- hi Irr~ ln 3 ltraquomfgtbc c -ud) 10 ~iIic ud hypudlumoptlJic 0CfUcirclC hftœJlUhranofcntco ewnn-d Omiddot1r 53 __phil ~ 1(] sncshyr olipmclM lAd 1ocJ fIgtCIIkIrIIr pplicd III idcatifT ltIre diffete_ 1 n ingttoloul - 1iIy f dl eazr- doM i _ a-doolcd Iioy ocM-gtocd Irt 0 11 -1 ~e ehofIC incrtoscd prodn ~yd lCgtlT _ ~lllaquo_ or upoocd middotdgteobiIc
id roamp_-J7 foom poych_ bccamc -- criItI PIIlico pudJ beauoc or 0flIGi cff III de=-~ p For ~ doc il CIl inctClhllC deoifc 10 IPPIy br phitoc ~ dacn Wh ovdI ~ i b r-w III dcoe Ioullliry oppu-iono d _ _ pc ltd Iowa lCODpcru l oudIo poogtshy
___ 1rnlPIliIiO P P _) ltH li__ tOC pel -J L T1lc pulp ond pope indulltry il IIM inrated 100 poiylllC-dclflllilll emct 110 lICrift Iow PC Sc1 ood PnxaRnl opplkMId ohe _ndl r e YOilbil PC ermyma
AltMOCri u of ny ens) r psyd plile 10 he ltl fil hido nllltie Ioi ft1
II lObOliry Il hpu wlIich cu lia pon CIlploJncd Irt tllc iumlc-d IC1Ocirc1lUumli r die le illl _1Opli1ilt ond Ill plilic nq-_ Th plO_ of cazyooa Iow oc_ eojeco of bull ] ia 11l-15~ Il h hea --cd hop iwJ lcsitlilioT il illed widl eued biJic d 1 det botmiddot ca bctItecn bUC Ind tinc bull Icircoldcltd ofal ohoc Neeukclc cre i n i_llia ber or r ~ N bullbull prolan e~
Meri _c1lebullbull-bullbull lilooo lb no ruIU ~ Icircol blI or an bc dilfc nl indop ~dy 116-111bull 11001 middotmiddotie of pn kd prope ar encrma r prrchroplralad cio Ipplicorgtou oon bc (ouad III 0 11Cicwo lm- ll~_Hlopliko con uibullbull ill hpcJieo lrobilO bf Illel obiJ to lnlO _le boJ__ _am 1cII u mdium Of POUUnl elllaridc (NoCl or Ka) up _n Ill ole _le m the ctniftln__menL AI 1 poole r- holophila M ltOpe illl ry hip oak _nnriooo le4- KCl ___
or ~ 1 Sa n_ ohS nlUJ~
The 1Ul- hne odopd bullbulln M 1 PO wc by ocqinnl eloocirc pt bct ar ocircI dlrpd ocld -ofilaa IO~
pfClipiIOUgraveOiL CoaUy le fi iP ~ lOI __bull dtc ilioy lIilic e_ ca nmiddot plllf ~middotil kllimn oppIlo 1ic r~~
H_ ptQpcny toc 1Ou n of by -
_ _ bullbull _ 115
1~h~ heur- bull _ _ bull 1Sl-Fr r _ c_ ___ Il _ r cmiddot bull __ -( d = t I~
MeIiPt1=bullbull eIEtel - _ pH _w mol lIrM ~ _ __ r c dot lI_c_ dclter _ Irillile pl ~ - Ilftd Iblt shyilic -pa io dieu oIMliry _1IW11 toI lllc lIIIluar-~ e-pn _ j lU al _
poltnfI ligt bull duo __ r cw-1I1 CI wtad han (ru- lit pli cn_ in he a el pt lia oncI llco le 1
_yIooa U- odoclt na - _ pH locII ____ _ ~ 110iallro C bullbulllbliph a-rir ~ r dln aiIilty bull 1 t c S _ - -rot __ j c Il PCa-r iIaIoC _ _ bullbullbullbull S _
t shy a IoococN_ _ 1-1 loi _ _ H middotrmiddotfH_ ___ bull Il llOl
OB ~ ncccdiloooM d icircnd tIoe 1iNI el du -u 1 Id lie -n dio OCII
- -shy
_- middot - n bull i lgtgtMf 1Ul d - _ _ - --shy X el
OlhIrtibam1 Ai xo_ _ _ 1 _1-
110
u p _ a _ _ pIOO -a_
t-S1lo) C ( b) t- T10c l 15 bull r- - nu ~ilco _ M No-shyrIldur bull _ bullbull dot lt1 0 el ~ il il n likcly dIIlt _ pa illoc will dolt PP- eI _ frual ud cuoclMflhiieo __MI
Exlr g~
TM ber 01 ee-co nilblc ~ 10 d Id bullbull rt Md iIeMi6lte ollOlacircll-l~cw-dybullbullbull 1co0l101__ r-AJnrcr Un cMifdt Ill IZ _ pd bullbull IJII dl f-ou =UIOI 1t jllr
_ ai dy bcJIC t -
o IrtiLtii ] bullbull _-_--- 15 bull fliTJ f bull 1 13IL _ bull SIKl shy(OUO) __ 1 ( r 1_ OUI _
t _ pnbk e 110 -lDNA-- bullbull JII 1 l _ _ m-doc bull - ou ~l~~_
r Ir -_ -doc __~ I
-Ic n _ bullbull iool no lllio _ ~ 110 dlc J S _ ~ ta U t n bullbullmiddot - 11 iIoc bull bull OH JI
b _ bplr lbull
ctD~ c ~ 1-lIl (qH ~ p lIlowa III Ile _ampIr 6cpcJidca _ p H= ltDo -Di- -nu r ___ Id ~ fftY lltd
III 1 fIltIICIloirlly dIIl pood
e-o _ Il _ItU
bull
Productkln lJl MZ)1Mo1 fovm ~
rlaquoriotuJ 0lt0Yf IMIMI i oIDNA ltII bc very bdprul ra rd lJIla1ion fil _fui euym ll_ebullbull Ile idaI_ of polUUIllIy fulnynICS i Iy lino 09 To uIIIlI _ of lbocirc kMtIt idUlrifMd ca u 10 bc de bullniloblc fol ~ dl u vi b 1yUc ic1IIgtd Iicu_ -~ _ _pu lot the pnocI__ofCIU) ~ cm WJa r pIOdrioa or me biouIY Ile ~ d bJ 1amp biocDuo PIIdIoltdon of dle hile AJ_lIYely die CC eocodi do bionulyst n bc 1 d OcircII iable toool
Eltbbullbull1OPhIt blom_ ptOductIon Spococircolixd cqooip of~ bull Plaquo40i 0 owla fficic_ oIopecific bionllJyst dl1y f Ul~lIilolnddiioniIDrmiddotmod f lloA _ ril 1 bc debullbull1opnr ond 0 ho bc Iddruoed inM Iou- or _ pIoocircl Hl-~ lM- J)looJ ioIl ft bc -cd OcircM lgty 09iI of b alipoougrave Of
br 1 obbullbull dfic rmn~ pnxcd (c fedmiddotIlIICh laquoII cclon 0lt nnllllUll cul ) 1amp tdcli opcucircol biolteoclOlt II bullbull ampu-lih rUl CI d ohu Ilw bc cd r pk tpecific ioIIroio Woo- 1000II 00 lie _Iopcd in rhbullbullulioo ofccloalopbila r pad orpolyJJum d (VGII~IOr opcc1lic req inshy W oIic b bol ore Iorcd he IoiJlb ra dl IIK dIbull-rillic 01 on i_n f lucircdIo 01lfClIIIi ioolcd 1IIdI dl _l~jl
Gene do-d orproducllon At p the fc _ pod n II101Y fol lMOIlpIlocirclu _ -ocirc6cltcd rd pmshyltcdn nurdon mil oI kinamp 1 Wy1aoOUcirctlbler rhnc laquoCII IIlO bc deyckI9cd ThbullbullI_inl rid bull i_ r lIIgtlidinJ amplt in pa1Ic ~ CI le n UMllve olmOlive lIlodlOltl- A jIlr ion or h carymbullbull ILo h r dl y 1 indalolppl _ 0 _ don Edma roJi uptaUgraveltgta -rou- ( T blc Z bull 1ll1I+lB
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---_ Md eka o ~ - cJiM ond Hu The objccti of ~ uld le po i id in Iectiloamp lie Ic buM YIII P pl ir dlbullbullnq- cd ampIlltOIyl-oon farltiolopol ocri dl Icotiod of a bo ri1 Il oold bol votdcd wh ro dl poodllttion or u llllla cn_ 0 IKioI bulldo Btit bull likcty 10 le Ie A dJlWIJoclt or- ampnIIlu-loucd - bullbullIe dri~11 hb Iel r odn p_ _ dl Il lIioll or ludo d ion 01 toshyrocou cene prod of i RelaquoI1 dshylu plOltC -dcJiciulbullbull lnrJu YC _ d bco prod1an leYeh or _c 1lIl~
s-ol 01 u _1lIncd buw ppliable ouroc 01 __ ribnlriu ln __~ a bc 01 dloic dI_ plin I+IwoI9) s--nhc~ i ufM rkk ofOcircOldOllioolmiddotbody ~ M do bullbull E Il - plOt pan biiugrave Ite Id1oo 1tc 1idd
nu ilobilil-r vlplOd 11I1 -po Iloo _d _ nd raiIooiq ~ propenico _y Il fNllCl CIcircCriItc d _dorrl ln fVIICUw diccud bull __ Iurion i ~a plt~1I elliccediliucy 01 oucll)tf Oftc 100 1icircIlnria (10_10 nrion) MW bc d nriollQ dcoftd lI5lUllod rc )
Condu_ne and penpecthon Eltnm uYC _- 101 in indQono pngtlaquolt ludiampriltltu~ cIIc1 nd ial opplia W _ ptOd 0 60 ftooooI rlgtc IIddlriool ploi oioa efltn-- TW lboI 100 ond te m iblc i odily bullbullpndi Il ba arcd tho I bu lOS lIf lin 1 1 bull tldncd CClI will bc ltul 1c nd _ rhe ocirc or _ upreooion dlllllIlu (cc dcTclopfncn of lt11 ud im _ Iopo kor _m) _~I 10- up 01 roltlt dil Ir i n_ lgtlc 10 CC op lib egrave f lhe diWIK _ Ir h llbl orn ned w11 r ond 1laquo11 tlc_ rion IIlc n œy will bol dl d la 00- _errraoom le u i ye b WOegrave p fiosoeOf e opecoftc WO 11y lricdly ocu deyclo 01 pooteOcircII Ocircllamp lItd dicd cYOIulioraquo cil bullbull oilI 10 iIl unitbullbull am Ocircl biocotolpic ilS wicll -ill iolaquoco tllo pp_ 01 bull f_ uraaopbil
~ altcNCfIn_~lt-flIlhIt_b1IliI ~(Mlbl_lollhe _1) IIH ioMlt ~ II aphlic OIll9flMII$lIM 9 _ Mon fA lIMue nNNl9tfle$ ~
_ - 1IIri Insighu lnta _ 1Ulttgt 1Ifo- pl 10laquo_1 JqJiIo IboocWrioL d cd ~ œnoIn ail _ -anboonollclf ~IDfODC SdiwIIlIU OW ID n IN _ bIrbo8OislJdbdtcI~9In__bullbull 0I1O
u ID _ Gmn bIutpmu ifonI _ PIfbm -11lt1 induumllt __rltlIldampul ail he-N
- 1
i 1 1 1
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DiIrofflY cl~OC- 0jlfkW MImiddotmiddot br_de 1 i aI au_dM _ pobiluumla Y for lIeOI cuyme lU pin ~=pta-itomair ilI lia~ 10 kttp down Ihc (Otef 0 pt(gtshy
dacrion m~~~tIw -1 xm_r= prodllCilll tamporr will rdUbly mm al PfOI Tho dtfkpllilaquo of ~ ufKlUriaa trdIniquco and thc le-
pm PfOIiUI Degtoota 01 he wshyet ~ known dirtard cYltgtlushy
OIlOl froaI
bon k Of 1 rudom nashyna the ~~al-
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iCI far _ ntnlOltf1OtO in shyaM lime olœrod choir iampioal Mm n raQoltlaJ dollp NId Edooniaa ppruiOCha prumi la
halllaquo emlII(Iamp)lIl Tbey aloo
Loi
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Extremophiles as a source for novel enzymes Bertus van den BlXg
M_ lObe_ shy on bull _
bo_~ __ _ ft _--lI)r_oI_pH TI-__ _0 No IIogtOIIbool lnl
1Ol __---shy~ 00 br l-lq lodulriIoI dc d fOI bioatayJ do lM COI _ cooodiltions id obIlt cfllwto ~ Nu -od co lh ~ rOI _h uyma cmoaI arpalc tloaio bIcircolIc31alyI emn lM boette cheinl pl oohilth con kod 10 _ cl5acircelo pnodian 01 10 gttltragtIumlODltIgtltB (cr bull tt iffNIrtlltlllll nkn Il ~ ~ilC Ibc ampa do ID do _ dun JOOO dlll_ euyma lM oori6ed or thcfCl dleir-~ iDdtauiol pIi_ he pracA UZ)1IM bon niU _ 1Iccediloe bull de A0a_ dl iam foct _y Iob euyma lN _ wilttltond ml ~
-Ji As rauk dM lt~ iu almia Ole ~ i~_C1I famp_ hot igt pcoc dcugrave oudo zac-phila gtIe _ cl ~1U 1~J~
Eure QOnd fCfc 10 pII)l coIre le _pc Je ndirlti_ bult ID _
_ u1gt Ii~ad pH (rhI Il loi vt doc couemovtoilco h bec idltn~d Olle bclonJ 0 1 01 Ile AIclI bull _bullbull bullbullny nunoophilc r dgtc ria ond uy roc i bullbull o Illy ItIcntlllcd od dlancuriud
TIoc _ion ilco C090ble rviY uncla no eootchfoollt __lion1 shy_nu h Icd _ doc _nies Mw _ becn Yod roc_ oondm- 1 d bull -idaob6c r do t do hbullbull hcgtIotood d IIlncucircofldy cboruotiocd r- lU pH bull HP _ lIcircIIn
1 ~ l aDd diocua 0CCCm pl bull shydlo d-IJ iIoNoliobullbullnd ~ 01 ~ r _pt-_shyAi illu cd ln Tobie 1 lM el licol COllall numiddot lcr widc iEry d~ middot diliom Iugravech pnilm odoptcd nc b Iyo i_ m- doc =p cauld bclBmiddot
IIIlpliablc ibltty dio-ne fToble 1) F do dep cL po)mcn dli _ bull _ r- dobullbull _ aetiw bull and - 10 ltiIlt ~nfCIl ohe prcfcrrcd Us- the _ lb ooIwl Ugtd __tIy oIIc litlt libiliq tbc boltnf p_ AltanoIJ ir lgtftC necbullbull pcltshyfoc RCf_peooilk odIl1cUcircltXI 0( ltOnfIOOlnd lof tbc lDdlais cL bull phomuautioaIly le pouduco Ofpnilt oohc IJ diIf nl ~i ply bull the 1Hoo1you AI II Icirci rclthcc --- ocorilty tIZflH m- loIcgtpIlilif le OIIOIld bc _ ibIc clioicc r pptaiDn i__-dia 1~5I n Ji ~ ofn i_mc lOI ~ di ltIfICplile c dopltcd off yaciriof fIlin rOf 0 ~riuy 01 oppli(otiofto
_ hM TIocpliilt CJIrcmophilco ~ cd _ ne rion kul umophlllc ptOIlt_ li poIy-dqndil lt110) IUCh n dlul h bull d yI ~Vll rotlCl lb IAlI Inod il _10 IThIc 1~ Tho fCO-o 1)1
Otobk nd oeil ol ~cd PfM- obtmiddot A icnd nou he oolubilify 01 noy ion laquogtngtpOfInll in penk ltr po)merlc oubouo bull liancly cd MOfco~ dobullbullioIr 01 11oft ludinl 0 undctifld 00000pliulOf 1 uh ed hipMr
Tbo 01 fta oIlhampl1llOpbik ee Irtrgtctltd mlEh lttenu- Sccnol duoc-dimcnMoMl _fU~ ho lcIcn lOigtlIltI oAd comd 11 m_ of coophilie tefJWtt wim Illagravemo ellOCOcircdoriooc ltIre - tfylll Ile-- hi Irr~ ln 3 ltraquomfgtbc c -ud) 10 ~iIic ud hypudlumoptlJic 0CfUcirclC hftœJlUhranofcntco ewnn-d Omiddot1r 53 __phil ~ 1(] sncshyr olipmclM lAd 1ocJ fIgtCIIkIrIIr pplicd III idcatifT ltIre diffete_ 1 n ingttoloul - 1iIy f dl eazr- doM i _ a-doolcd Iioy ocM-gtocd Irt 0 11 -1 ~e ehofIC incrtoscd prodn ~yd lCgtlT _ ~lllaquo_ or upoocd middotdgteobiIc
id roamp_-J7 foom poych_ bccamc -- criItI PIIlico pudJ beauoc or 0flIGi cff III de=-~ p For ~ doc il CIl inctClhllC deoifc 10 IPPIy br phitoc ~ dacn Wh ovdI ~ i b r-w III dcoe Ioullliry oppu-iono d _ _ pc ltd Iowa lCODpcru l oudIo poogtshy
___ 1rnlPIliIiO P P _) ltH li__ tOC pel -J L T1lc pulp ond pope indulltry il IIM inrated 100 poiylllC-dclflllilll emct 110 lICrift Iow PC Sc1 ood PnxaRnl opplkMId ohe _ndl r e YOilbil PC ermyma
AltMOCri u of ny ens) r psyd plile 10 he ltl fil hido nllltie Ioi ft1
II lObOliry Il hpu wlIich cu lia pon CIlploJncd Irt tllc iumlc-d IC1Ocirc1lUumli r die le illl _1Opli1ilt ond Ill plilic nq-_ Th plO_ of cazyooa Iow oc_ eojeco of bull ] ia 11l-15~ Il h hea --cd hop iwJ lcsitlilioT il illed widl eued biJic d 1 det botmiddot ca bctItecn bUC Ind tinc bull Icircoldcltd ofal ohoc Neeukclc cre i n i_llia ber or r ~ N bullbull prolan e~
Meri _c1lebullbull-bullbull lilooo lb no ruIU ~ Icircol blI or an bc dilfc nl indop ~dy 116-111bull 11001 middotmiddotie of pn kd prope ar encrma r prrchroplralad cio Ipplicorgtou oon bc (ouad III 0 11Cicwo lm- ll~_Hlopliko con uibullbull ill hpcJieo lrobilO bf Illel obiJ to lnlO _le boJ__ _am 1cII u mdium Of POUUnl elllaridc (NoCl or Ka) up _n Ill ole _le m the ctniftln__menL AI 1 poole r- holophila M ltOpe illl ry hip oak _nnriooo le4- KCl ___
or ~ 1 Sa n_ ohS nlUJ~
The 1Ul- hne odopd bullbulln M 1 PO wc by ocqinnl eloocirc pt bct ar ocircI dlrpd ocld -ofilaa IO~
pfClipiIOUgraveOiL CoaUy le fi iP ~ lOI __bull dtc ilioy lIilic e_ ca nmiddot plllf ~middotil kllimn oppIlo 1ic r~~
H_ ptQpcny toc 1Ou n of by -
_ _ bullbull _ 115
1~h~ heur- bull _ _ bull 1Sl-Fr r _ c_ ___ Il _ r cmiddot bull __ -( d = t I~
MeIiPt1=bullbull eIEtel - _ pH _w mol lIrM ~ _ __ r c dot lI_c_ dclter _ Irillile pl ~ - Ilftd Iblt shyilic -pa io dieu oIMliry _1IW11 toI lllc lIIIluar-~ e-pn _ j lU al _
poltnfI ligt bull duo __ r cw-1I1 CI wtad han (ru- lit pli cn_ in he a el pt lia oncI llco le 1
_yIooa U- odoclt na - _ pH locII ____ _ ~ 110iallro C bullbulllbliph a-rir ~ r dln aiIilty bull 1 t c S _ - -rot __ j c Il PCa-r iIaIoC _ _ bullbullbullbull S _
t shy a IoococN_ _ 1-1 loi _ _ H middotrmiddotfH_ ___ bull Il llOl
OB ~ ncccdiloooM d icircnd tIoe 1iNI el du -u 1 Id lie -n dio OCII
- -shy
_- middot - n bull i lgtgtMf 1Ul d - _ _ - --shy X el
OlhIrtibam1 Ai xo_ _ _ 1 _1-
110
u p _ a _ _ pIOO -a_
t-S1lo) C ( b) t- T10c l 15 bull r- - nu ~ilco _ M No-shyrIldur bull _ bullbull dot lt1 0 el ~ il il n likcly dIIlt _ pa illoc will dolt PP- eI _ frual ud cuoclMflhiieo __MI
Exlr g~
TM ber 01 ee-co nilblc ~ 10 d Id bullbull rt Md iIeMi6lte ollOlacircll-l~cw-dybullbullbull 1co0l101__ r-AJnrcr Un cMifdt Ill IZ _ pd bullbull IJII dl f-ou =UIOI 1t jllr
_ ai dy bcJIC t -
o IrtiLtii ] bullbull _-_--- 15 bull fliTJ f bull 1 13IL _ bull SIKl shy(OUO) __ 1 ( r 1_ OUI _
t _ pnbk e 110 -lDNA-- bullbull JII 1 l _ _ m-doc bull - ou ~l~~_
r Ir -_ -doc __~ I
-Ic n _ bullbull iool no lllio _ ~ 110 dlc J S _ ~ ta U t n bullbullmiddot - 11 iIoc bull bull OH JI
b _ bplr lbull
ctD~ c ~ 1-lIl (qH ~ p lIlowa III Ile _ampIr 6cpcJidca _ p H= ltDo -Di- -nu r ___ Id ~ fftY lltd
III 1 fIltIICIloirlly dIIl pood
e-o _ Il _ItU
bull
Productkln lJl MZ)1Mo1 fovm ~
rlaquoriotuJ 0lt0Yf IMIMI i oIDNA ltII bc very bdprul ra rd lJIla1ion fil _fui euym ll_ebullbull Ile idaI_ of polUUIllIy fulnynICS i Iy lino 09 To uIIIlI _ of lbocirc kMtIt idUlrifMd ca u 10 bc de bullniloblc fol ~ dl u vi b 1yUc ic1IIgtd Iicu_ -~ _ _pu lot the pnocI__ofCIU) ~ cm WJa r pIOdrioa or me biouIY Ile ~ d bJ 1amp biocDuo PIIdIoltdon of dle hile AJ_lIYely die CC eocodi do bionulyst n bc 1 d OcircII iable toool
Eltbbullbull1OPhIt blom_ ptOductIon Spococircolixd cqooip of~ bull Plaquo40i 0 owla fficic_ oIopecific bionllJyst dl1y f Ul~lIilolnddiioniIDrmiddotmod f lloA _ ril 1 bc debullbull1opnr ond 0 ho bc Iddruoed inM Iou- or _ pIoocircl Hl-~ lM- J)looJ ioIl ft bc -cd OcircM lgty 09iI of b alipoougrave Of
br 1 obbullbull dfic rmn~ pnxcd (c fedmiddotIlIICh laquoII cclon 0lt nnllllUll cul ) 1amp tdcli opcucircol biolteoclOlt II bullbull ampu-lih rUl CI d ohu Ilw bc cd r pk tpecific ioIIroio Woo- 1000II 00 lie _Iopcd in rhbullbullulioo ofccloalopbila r pad orpolyJJum d (VGII~IOr opcc1lic req inshy W oIic b bol ore Iorcd he IoiJlb ra dl IIK dIbull-rillic 01 on i_n f lucircdIo 01lfClIIIi ioolcd 1IIdI dl _l~jl
Gene do-d orproducllon At p the fc _ pod n II101Y fol lMOIlpIlocirclu _ -ocirc6cltcd rd pmshyltcdn nurdon mil oI kinamp 1 Wy1aoOUcirctlbler rhnc laquoCII IIlO bc deyckI9cd ThbullbullI_inl rid bull i_ r lIIgtlidinJ amplt in pa1Ic ~ CI le n UMllve olmOlive lIlodlOltl- A jIlr ion or h carymbullbull ILo h r dl y 1 indalolppl _ 0 _ don Edma roJi uptaUgraveltgta -rou- ( T blc Z bull 1ll1I+lB
Gribullbullool for 1 (01i1 ete Wl 11 docw opcd nlt y p1aid vctt1HI iDd illk lue Pp ocircbic 1 c p dllCUcircMl 0( ilegrave nq rili d dlc ctllodaoaN01_ E _ bullbullbull folio ta Il~1~ H dl middotenmiddotk_n pbc__ 01 im_-locidy r_lion by 0 middot d p_oocirc i t nli 1lt1 fOlCC h ra oI i iflaUil_Ir M 11 _bco of ugravew nllbk ncludil bonc101 11 111 dI ~a ~ ~h
---_ Md eka o ~ - cJiM ond Hu The objccti of ~ uld le po i id in Iectiloamp lie Ic buM YIII P pl ir dlbullbullnq- cd ampIlltOIyl-oon farltiolopol ocri dl Icotiod of a bo ri1 Il oold bol votdcd wh ro dl poodllttion or u llllla cn_ 0 IKioI bulldo Btit bull likcty 10 le Ie A dJlWIJoclt or- ampnIIlu-loucd - bullbullIe dri~11 hb Iel r odn p_ _ dl Il lIioll or ludo d ion 01 toshyrocou cene prod of i RelaquoI1 dshylu plOltC -dcJiciulbullbull lnrJu YC _ d bco prod1an leYeh or _c 1lIl~
s-ol 01 u _1lIncd buw ppliable ouroc 01 __ ribnlriu ln __~ a bc 01 dloic dI_ plin I+IwoI9) s--nhc~ i ufM rkk ofOcircOldOllioolmiddotbody ~ M do bullbull E Il - plOt pan biiugrave Ite Id1oo 1tc 1idd
nu ilobilil-r vlplOd 11I1 -po Iloo _d _ nd raiIooiq ~ propenico _y Il fNllCl CIcircCriItc d _dorrl ln fVIICUw diccud bull __ Iurion i ~a plt~1I elliccediliucy 01 oucll)tf Oftc 100 1icircIlnria (10_10 nrion) MW bc d nriollQ dcoftd lI5lUllod rc )
Condu_ne and penpecthon Eltnm uYC _- 101 in indQono pngtlaquolt ludiampriltltu~ cIIc1 nd ial opplia W _ ptOd 0 60 ftooooI rlgtc IIddlriool ploi oioa efltn-- TW lboI 100 ond te m iblc i odily bullbullpndi Il ba arcd tho I bu lOS lIf lin 1 1 bull tldncd CClI will bc ltul 1c nd _ rhe ocirc or _ upreooion dlllllIlu (cc dcTclopfncn of lt11 ud im _ Iopo kor _m) _~I 10- up 01 roltlt dil Ir i n_ lgtlc 10 CC op lib egrave f lhe diWIK _ Ir h llbl orn ned w11 r ond 1laquo11 tlc_ rion IIlc n œy will bol dl d la 00- _errraoom le u i ye b WOegrave p fiosoeOf e opecoftc WO 11y lricdly ocu deyclo 01 pooteOcircII Ocircllamp lItd dicd cYOIulioraquo cil bullbull oilI 10 iIl unitbullbull am Ocircl biocotolpic ilS wicll -ill iolaquoco tllo pp_ 01 bull f_ uraaopbil
1Ol __---shy~ 00 br l-lq lodulriIoI dc d fOI bioatayJ do lM COI _ cooodiltions id obIlt cfllwto ~ Nu -od co lh ~ rOI _h uyma cmoaI arpalc tloaio bIcircolIc31alyI emn lM boette cheinl pl oohilth con kod 10 _ cl5acircelo pnodian 01 10 gttltragtIumlODltIgtltB (cr bull tt iffNIrtlltlllll nkn Il ~ ~ilC Ibc ampa do ID do _ dun JOOO dlll_ euyma lM oori6ed or thcfCl dleir-~ iDdtauiol pIi_ he pracA UZ)1IM bon niU _ 1Iccediloe bull de A0a_ dl iam foct _y Iob euyma lN _ wilttltond ml ~
-Ji As rauk dM lt~ iu almia Ole ~ i~_C1I famp_ hot igt pcoc dcugrave oudo zac-phila gtIe _ cl ~1U 1~J~
Eure QOnd fCfc 10 pII)l coIre le _pc Je ndirlti_ bult ID _
_ u1gt Ii~ad pH (rhI Il loi vt doc couemovtoilco h bec idltn~d Olle bclonJ 0 1 01 Ile AIclI bull _bullbull bullbullny nunoophilc r dgtc ria ond uy roc i bullbull o Illy ItIcntlllcd od dlancuriud
TIoc _ion ilco C090ble rviY uncla no eootchfoollt __lion1 shy_nu h Icd _ doc _nies Mw _ becn Yod roc_ oondm- 1 d bull -idaob6c r do t do hbullbull hcgtIotood d IIlncucircofldy cboruotiocd r- lU pH bull HP _ lIcircIIn
1 ~ l aDd diocua 0CCCm pl bull shydlo d-IJ iIoNoliobullbullnd ~ 01 ~ r _pt-_shyAi illu cd ln Tobie 1 lM el licol COllall numiddot lcr widc iEry d~ middot diliom Iugravech pnilm odoptcd nc b Iyo i_ m- doc =p cauld bclBmiddot
IIIlpliablc ibltty dio-ne fToble 1) F do dep cL po)mcn dli _ bull _ r- dobullbull _ aetiw bull and - 10 ltiIlt ~nfCIl ohe prcfcrrcd Us- the _ lb ooIwl Ugtd __tIy oIIc litlt libiliq tbc boltnf p_ AltanoIJ ir lgtftC necbullbull pcltshyfoc RCf_peooilk odIl1cUcircltXI 0( ltOnfIOOlnd lof tbc lDdlais cL bull phomuautioaIly le pouduco Ofpnilt oohc IJ diIf nl ~i ply bull the 1Hoo1you AI II Icirci rclthcc --- ocorilty tIZflH m- loIcgtpIlilif le OIIOIld bc _ ibIc clioicc r pptaiDn i__-dia 1~5I n Ji ~ ofn i_mc lOI ~ di ltIfICplile c dopltcd off yaciriof fIlin rOf 0 ~riuy 01 oppli(otiofto
_ hM TIocpliilt CJIrcmophilco ~ cd _ ne rion kul umophlllc ptOIlt_ li poIy-dqndil lt110) IUCh n dlul h bull d yI ~Vll rotlCl lb IAlI Inod il _10 IThIc 1~ Tho fCO-o 1)1
Otobk nd oeil ol ~cd PfM- obtmiddot A icnd nou he oolubilify 01 noy ion laquogtngtpOfInll in penk ltr po)merlc oubouo bull liancly cd MOfco~ dobullbullioIr 01 11oft ludinl 0 undctifld 00000pliulOf 1 uh ed hipMr
Tbo 01 fta oIlhampl1llOpbik ee Irtrgtctltd mlEh lttenu- Sccnol duoc-dimcnMoMl _fU~ ho lcIcn lOigtlIltI oAd comd 11 m_ of coophilie tefJWtt wim Illagravemo ellOCOcircdoriooc ltIre - tfylll Ile-- hi Irr~ ln 3 ltraquomfgtbc c -ud) 10 ~iIic ud hypudlumoptlJic 0CfUcirclC hftœJlUhranofcntco ewnn-d Omiddot1r 53 __phil ~ 1(] sncshyr olipmclM lAd 1ocJ fIgtCIIkIrIIr pplicd III idcatifT ltIre diffete_ 1 n ingttoloul - 1iIy f dl eazr- doM i _ a-doolcd Iioy ocM-gtocd Irt 0 11 -1 ~e ehofIC incrtoscd prodn ~yd lCgtlT _ ~lllaquo_ or upoocd middotdgteobiIc
id roamp_-J7 foom poych_ bccamc -- criItI PIIlico pudJ beauoc or 0flIGi cff III de=-~ p For ~ doc il CIl inctClhllC deoifc 10 IPPIy br phitoc ~ dacn Wh ovdI ~ i b r-w III dcoe Ioullliry oppu-iono d _ _ pc ltd Iowa lCODpcru l oudIo poogtshy
___ 1rnlPIliIiO P P _) ltH li__ tOC pel -J L T1lc pulp ond pope indulltry il IIM inrated 100 poiylllC-dclflllilll emct 110 lICrift Iow PC Sc1 ood PnxaRnl opplkMId ohe _ndl r e YOilbil PC ermyma
AltMOCri u of ny ens) r psyd plile 10 he ltl fil hido nllltie Ioi ft1
II lObOliry Il hpu wlIich cu lia pon CIlploJncd Irt tllc iumlc-d IC1Ocirc1lUumli r die le illl _1Opli1ilt ond Ill plilic nq-_ Th plO_ of cazyooa Iow oc_ eojeco of bull ] ia 11l-15~ Il h hea --cd hop iwJ lcsitlilioT il illed widl eued biJic d 1 det botmiddot ca bctItecn bUC Ind tinc bull Icircoldcltd ofal ohoc Neeukclc cre i n i_llia ber or r ~ N bullbull prolan e~
Meri _c1lebullbull-bullbull lilooo lb no ruIU ~ Icircol blI or an bc dilfc nl indop ~dy 116-111bull 11001 middotmiddotie of pn kd prope ar encrma r prrchroplralad cio Ipplicorgtou oon bc (ouad III 0 11Cicwo lm- ll~_Hlopliko con uibullbull ill hpcJieo lrobilO bf Illel obiJ to lnlO _le boJ__ _am 1cII u mdium Of POUUnl elllaridc (NoCl or Ka) up _n Ill ole _le m the ctniftln__menL AI 1 poole r- holophila M ltOpe illl ry hip oak _nnriooo le4- KCl ___
or ~ 1 Sa n_ ohS nlUJ~
The 1Ul- hne odopd bullbulln M 1 PO wc by ocqinnl eloocirc pt bct ar ocircI dlrpd ocld -ofilaa IO~
pfClipiIOUgraveOiL CoaUy le fi iP ~ lOI __bull dtc ilioy lIilic e_ ca nmiddot plllf ~middotil kllimn oppIlo 1ic r~~
H_ ptQpcny toc 1Ou n of by -
_ _ bullbull _ 115
1~h~ heur- bull _ _ bull 1Sl-Fr r _ c_ ___ Il _ r cmiddot bull __ -( d = t I~
MeIiPt1=bullbull eIEtel - _ pH _w mol lIrM ~ _ __ r c dot lI_c_ dclter _ Irillile pl ~ - Ilftd Iblt shyilic -pa io dieu oIMliry _1IW11 toI lllc lIIIluar-~ e-pn _ j lU al _
poltnfI ligt bull duo __ r cw-1I1 CI wtad han (ru- lit pli cn_ in he a el pt lia oncI llco le 1
_yIooa U- odoclt na - _ pH locII ____ _ ~ 110iallro C bullbulllbliph a-rir ~ r dln aiIilty bull 1 t c S _ - -rot __ j c Il PCa-r iIaIoC _ _ bullbullbullbull S _
t shy a IoococN_ _ 1-1 loi _ _ H middotrmiddotfH_ ___ bull Il llOl
OB ~ ncccdiloooM d icircnd tIoe 1iNI el du -u 1 Id lie -n dio OCII
- -shy
_- middot - n bull i lgtgtMf 1Ul d - _ _ - --shy X el
OlhIrtibam1 Ai xo_ _ _ 1 _1-
110
u p _ a _ _ pIOO -a_
t-S1lo) C ( b) t- T10c l 15 bull r- - nu ~ilco _ M No-shyrIldur bull _ bullbull dot lt1 0 el ~ il il n likcly dIIlt _ pa illoc will dolt PP- eI _ frual ud cuoclMflhiieo __MI
Exlr g~
TM ber 01 ee-co nilblc ~ 10 d Id bullbull rt Md iIeMi6lte ollOlacircll-l~cw-dybullbullbull 1co0l101__ r-AJnrcr Un cMifdt Ill IZ _ pd bullbull IJII dl f-ou =UIOI 1t jllr
_ ai dy bcJIC t -
o IrtiLtii ] bullbull _-_--- 15 bull fliTJ f bull 1 13IL _ bull SIKl shy(OUO) __ 1 ( r 1_ OUI _
t _ pnbk e 110 -lDNA-- bullbull JII 1 l _ _ m-doc bull - ou ~l~~_
r Ir -_ -doc __~ I
-Ic n _ bullbull iool no lllio _ ~ 110 dlc J S _ ~ ta U t n bullbullmiddot - 11 iIoc bull bull OH JI
b _ bplr lbull
ctD~ c ~ 1-lIl (qH ~ p lIlowa III Ile _ampIr 6cpcJidca _ p H= ltDo -Di- -nu r ___ Id ~ fftY lltd
III 1 fIltIICIloirlly dIIl pood
e-o _ Il _ItU
bull
Productkln lJl MZ)1Mo1 fovm ~
rlaquoriotuJ 0lt0Yf IMIMI i oIDNA ltII bc very bdprul ra rd lJIla1ion fil _fui euym ll_ebullbull Ile idaI_ of polUUIllIy fulnynICS i Iy lino 09 To uIIIlI _ of lbocirc kMtIt idUlrifMd ca u 10 bc de bullniloblc fol ~ dl u vi b 1yUc ic1IIgtd Iicu_ -~ _ _pu lot the pnocI__ofCIU) ~ cm WJa r pIOdrioa or me biouIY Ile ~ d bJ 1amp biocDuo PIIdIoltdon of dle hile AJ_lIYely die CC eocodi do bionulyst n bc 1 d OcircII iable toool
Eltbbullbull1OPhIt blom_ ptOductIon Spococircolixd cqooip of~ bull Plaquo40i 0 owla fficic_ oIopecific bionllJyst dl1y f Ul~lIilolnddiioniIDrmiddotmod f lloA _ ril 1 bc debullbull1opnr ond 0 ho bc Iddruoed inM Iou- or _ pIoocircl Hl-~ lM- J)looJ ioIl ft bc -cd OcircM lgty 09iI of b alipoougrave Of
br 1 obbullbull dfic rmn~ pnxcd (c fedmiddotIlIICh laquoII cclon 0lt nnllllUll cul ) 1amp tdcli opcucircol biolteoclOlt II bullbull ampu-lih rUl CI d ohu Ilw bc cd r pk tpecific ioIIroio Woo- 1000II 00 lie _Iopcd in rhbullbullulioo ofccloalopbila r pad orpolyJJum d (VGII~IOr opcc1lic req inshy W oIic b bol ore Iorcd he IoiJlb ra dl IIK dIbull-rillic 01 on i_n f lucircdIo 01lfClIIIi ioolcd 1IIdI dl _l~jl
Gene do-d orproducllon At p the fc _ pod n II101Y fol lMOIlpIlocirclu _ -ocirc6cltcd rd pmshyltcdn nurdon mil oI kinamp 1 Wy1aoOUcirctlbler rhnc laquoCII IIlO bc deyckI9cd ThbullbullI_inl rid bull i_ r lIIgtlidinJ amplt in pa1Ic ~ CI le n UMllve olmOlive lIlodlOltl- A jIlr ion or h carymbullbull ILo h r dl y 1 indalolppl _ 0 _ don Edma roJi uptaUgraveltgta -rou- ( T blc Z bull 1ll1I+lB
Gribullbullool for 1 (01i1 ete Wl 11 docw opcd nlt y p1aid vctt1HI iDd illk lue Pp ocircbic 1 c p dllCUcircMl 0( ilegrave nq rili d dlc ctllodaoaN01_ E _ bullbullbull folio ta Il~1~ H dl middotenmiddotk_n pbc__ 01 im_-locidy r_lion by 0 middot d p_oocirc i t nli 1lt1 fOlCC h ra oI i iflaUil_Ir M 11 _bco of ugravew nllbk ncludil bonc101 11 111 dI ~a ~ ~h
---_ Md eka o ~ - cJiM ond Hu The objccti of ~ uld le po i id in Iectiloamp lie Ic buM YIII P pl ir dlbullbullnq- cd ampIlltOIyl-oon farltiolopol ocri dl Icotiod of a bo ri1 Il oold bol votdcd wh ro dl poodllttion or u llllla cn_ 0 IKioI bulldo Btit bull likcty 10 le Ie A dJlWIJoclt or- ampnIIlu-loucd - bullbullIe dri~11 hb Iel r odn p_ _ dl Il lIioll or ludo d ion 01 toshyrocou cene prod of i RelaquoI1 dshylu plOltC -dcJiciulbullbull lnrJu YC _ d bco prod1an leYeh or _c 1lIl~
s-ol 01 u _1lIncd buw ppliable ouroc 01 __ ribnlriu ln __~ a bc 01 dloic dI_ plin I+IwoI9) s--nhc~ i ufM rkk ofOcircOldOllioolmiddotbody ~ M do bullbull E Il - plOt pan biiugrave Ite Id1oo 1tc 1idd
nu ilobilil-r vlplOd 11I1 -po Iloo _d _ nd raiIooiq ~ propenico _y Il fNllCl CIcircCriItc d _dorrl ln fVIICUw diccud bull __ Iurion i ~a plt~1I elliccediliucy 01 oucll)tf Oftc 100 1icircIlnria (10_10 nrion) MW bc d nriollQ dcoftd lI5lUllod rc )
Condu_ne and penpecthon Eltnm uYC _- 101 in indQono pngtlaquolt ludiampriltltu~ cIIc1 nd ial opplia W _ ptOd 0 60 ftooooI rlgtc IIddlriool ploi oioa efltn-- TW lboI 100 ond te m iblc i odily bullbullpndi Il ba arcd tho I bu lOS lIf lin 1 1 bull tldncd CClI will bc ltul 1c nd _ rhe ocirc or _ upreooion dlllllIlu (cc dcTclopfncn of lt11 ud im _ Iopo kor _m) _~I 10- up 01 roltlt dil Ir i n_ lgtlc 10 CC op lib egrave f lhe diWIK _ Ir h llbl orn ned w11 r ond 1laquo11 tlc_ rion IIlc n œy will bol dl d la 00- _errraoom le u i ye b WOegrave p fiosoeOf e opecoftc WO 11y lricdly ocu deyclo 01 pooteOcircII Ocircllamp lItd dicd cYOIulioraquo cil bullbull oilI 10 iIl unitbullbull am Ocircl biocotolpic ilS wicll -ill iolaquoco tllo pp_ 01 bull f_ uraaopbil
Tbo 01 fta oIlhampl1llOpbik ee Irtrgtctltd mlEh lttenu- Sccnol duoc-dimcnMoMl _fU~ ho lcIcn lOigtlIltI oAd comd 11 m_ of coophilie tefJWtt wim Illagravemo ellOCOcircdoriooc ltIre - tfylll Ile-- hi Irr~ ln 3 ltraquomfgtbc c -ud) 10 ~iIic ud hypudlumoptlJic 0CfUcirclC hftœJlUhranofcntco ewnn-d Omiddot1r 53 __phil ~ 1(] sncshyr olipmclM lAd 1ocJ fIgtCIIkIrIIr pplicd III idcatifT ltIre diffete_ 1 n ingttoloul - 1iIy f dl eazr- doM i _ a-doolcd Iioy ocM-gtocd Irt 0 11 -1 ~e ehofIC incrtoscd prodn ~yd lCgtlT _ ~lllaquo_ or upoocd middotdgteobiIc
id roamp_-J7 foom poych_ bccamc -- criItI PIIlico pudJ beauoc or 0flIGi cff III de=-~ p For ~ doc il CIl inctClhllC deoifc 10 IPPIy br phitoc ~ dacn Wh ovdI ~ i b r-w III dcoe Ioullliry oppu-iono d _ _ pc ltd Iowa lCODpcru l oudIo poogtshy
___ 1rnlPIliIiO P P _) ltH li__ tOC pel -J L T1lc pulp ond pope indulltry il IIM inrated 100 poiylllC-dclflllilll emct 110 lICrift Iow PC Sc1 ood PnxaRnl opplkMId ohe _ndl r e YOilbil PC ermyma
AltMOCri u of ny ens) r psyd plile 10 he ltl fil hido nllltie Ioi ft1
II lObOliry Il hpu wlIich cu lia pon CIlploJncd Irt tllc iumlc-d IC1Ocirc1lUumli r die le illl _1Opli1ilt ond Ill plilic nq-_ Th plO_ of cazyooa Iow oc_ eojeco of bull ] ia 11l-15~ Il h hea --cd hop iwJ lcsitlilioT il illed widl eued biJic d 1 det botmiddot ca bctItecn bUC Ind tinc bull Icircoldcltd ofal ohoc Neeukclc cre i n i_llia ber or r ~ N bullbull prolan e~
Meri _c1lebullbull-bullbull lilooo lb no ruIU ~ Icircol blI or an bc dilfc nl indop ~dy 116-111bull 11001 middotmiddotie of pn kd prope ar encrma r prrchroplralad cio Ipplicorgtou oon bc (ouad III 0 11Cicwo lm- ll~_Hlopliko con uibullbull ill hpcJieo lrobilO bf Illel obiJ to lnlO _le boJ__ _am 1cII u mdium Of POUUnl elllaridc (NoCl or Ka) up _n Ill ole _le m the ctniftln__menL AI 1 poole r- holophila M ltOpe illl ry hip oak _nnriooo le4- KCl ___
or ~ 1 Sa n_ ohS nlUJ~
The 1Ul- hne odopd bullbulln M 1 PO wc by ocqinnl eloocirc pt bct ar ocircI dlrpd ocld -ofilaa IO~
pfClipiIOUgraveOiL CoaUy le fi iP ~ lOI __bull dtc ilioy lIilic e_ ca nmiddot plllf ~middotil kllimn oppIlo 1ic r~~
H_ ptQpcny toc 1Ou n of by -
_ _ bullbull _ 115
1~h~ heur- bull _ _ bull 1Sl-Fr r _ c_ ___ Il _ r cmiddot bull __ -( d = t I~
MeIiPt1=bullbull eIEtel - _ pH _w mol lIrM ~ _ __ r c dot lI_c_ dclter _ Irillile pl ~ - Ilftd Iblt shyilic -pa io dieu oIMliry _1IW11 toI lllc lIIIluar-~ e-pn _ j lU al _
poltnfI ligt bull duo __ r cw-1I1 CI wtad han (ru- lit pli cn_ in he a el pt lia oncI llco le 1
_yIooa U- odoclt na - _ pH locII ____ _ ~ 110iallro C bullbulllbliph a-rir ~ r dln aiIilty bull 1 t c S _ - -rot __ j c Il PCa-r iIaIoC _ _ bullbullbullbull S _
t shy a IoococN_ _ 1-1 loi _ _ H middotrmiddotfH_ ___ bull Il llOl
OB ~ ncccdiloooM d icircnd tIoe 1iNI el du -u 1 Id lie -n dio OCII
- -shy
_- middot - n bull i lgtgtMf 1Ul d - _ _ - --shy X el
OlhIrtibam1 Ai xo_ _ _ 1 _1-
110
u p _ a _ _ pIOO -a_
t-S1lo) C ( b) t- T10c l 15 bull r- - nu ~ilco _ M No-shyrIldur bull _ bullbull dot lt1 0 el ~ il il n likcly dIIlt _ pa illoc will dolt PP- eI _ frual ud cuoclMflhiieo __MI
Exlr g~
TM ber 01 ee-co nilblc ~ 10 d Id bullbull rt Md iIeMi6lte ollOlacircll-l~cw-dybullbullbull 1co0l101__ r-AJnrcr Un cMifdt Ill IZ _ pd bullbull IJII dl f-ou =UIOI 1t jllr
_ ai dy bcJIC t -
o IrtiLtii ] bullbull _-_--- 15 bull fliTJ f bull 1 13IL _ bull SIKl shy(OUO) __ 1 ( r 1_ OUI _
t _ pnbk e 110 -lDNA-- bullbull JII 1 l _ _ m-doc bull - ou ~l~~_
r Ir -_ -doc __~ I
-Ic n _ bullbull iool no lllio _ ~ 110 dlc J S _ ~ ta U t n bullbullmiddot - 11 iIoc bull bull OH JI
b _ bplr lbull
ctD~ c ~ 1-lIl (qH ~ p lIlowa III Ile _ampIr 6cpcJidca _ p H= ltDo -Di- -nu r ___ Id ~ fftY lltd
III 1 fIltIICIloirlly dIIl pood
e-o _ Il _ItU
bull
Productkln lJl MZ)1Mo1 fovm ~
rlaquoriotuJ 0lt0Yf IMIMI i oIDNA ltII bc very bdprul ra rd lJIla1ion fil _fui euym ll_ebullbull Ile idaI_ of polUUIllIy fulnynICS i Iy lino 09 To uIIIlI _ of lbocirc kMtIt idUlrifMd ca u 10 bc de bullniloblc fol ~ dl u vi b 1yUc ic1IIgtd Iicu_ -~ _ _pu lot the pnocI__ofCIU) ~ cm WJa r pIOdrioa or me biouIY Ile ~ d bJ 1amp biocDuo PIIdIoltdon of dle hile AJ_lIYely die CC eocodi do bionulyst n bc 1 d OcircII iable toool
Eltbbullbull1OPhIt blom_ ptOductIon Spococircolixd cqooip of~ bull Plaquo40i 0 owla fficic_ oIopecific bionllJyst dl1y f Ul~lIilolnddiioniIDrmiddotmod f lloA _ ril 1 bc debullbull1opnr ond 0 ho bc Iddruoed inM Iou- or _ pIoocircl Hl-~ lM- J)looJ ioIl ft bc -cd OcircM lgty 09iI of b alipoougrave Of
br 1 obbullbull dfic rmn~ pnxcd (c fedmiddotIlIICh laquoII cclon 0lt nnllllUll cul ) 1amp tdcli opcucircol biolteoclOlt II bullbull ampu-lih rUl CI d ohu Ilw bc cd r pk tpecific ioIIroio Woo- 1000II 00 lie _Iopcd in rhbullbullulioo ofccloalopbila r pad orpolyJJum d (VGII~IOr opcc1lic req inshy W oIic b bol ore Iorcd he IoiJlb ra dl IIK dIbull-rillic 01 on i_n f lucircdIo 01lfClIIIi ioolcd 1IIdI dl _l~jl
Gene do-d orproducllon At p the fc _ pod n II101Y fol lMOIlpIlocirclu _ -ocirc6cltcd rd pmshyltcdn nurdon mil oI kinamp 1 Wy1aoOUcirctlbler rhnc laquoCII IIlO bc deyckI9cd ThbullbullI_inl rid bull i_ r lIIgtlidinJ amplt in pa1Ic ~ CI le n UMllve olmOlive lIlodlOltl- A jIlr ion or h carymbullbull ILo h r dl y 1 indalolppl _ 0 _ don Edma roJi uptaUgraveltgta -rou- ( T blc Z bull 1ll1I+lB
Gribullbullool for 1 (01i1 ete Wl 11 docw opcd nlt y p1aid vctt1HI iDd illk lue Pp ocircbic 1 c p dllCUcircMl 0( ilegrave nq rili d dlc ctllodaoaN01_ E _ bullbullbull folio ta Il~1~ H dl middotenmiddotk_n pbc__ 01 im_-locidy r_lion by 0 middot d p_oocirc i t nli 1lt1 fOlCC h ra oI i iflaUil_Ir M 11 _bco of ugravew nllbk ncludil bonc101 11 111 dI ~a ~ ~h
---_ Md eka o ~ - cJiM ond Hu The objccti of ~ uld le po i id in Iectiloamp lie Ic buM YIII P pl ir dlbullbullnq- cd ampIlltOIyl-oon farltiolopol ocri dl Icotiod of a bo ri1 Il oold bol votdcd wh ro dl poodllttion or u llllla cn_ 0 IKioI bulldo Btit bull likcty 10 le Ie A dJlWIJoclt or- ampnIIlu-loucd - bullbullIe dri~11 hb Iel r odn p_ _ dl Il lIioll or ludo d ion 01 toshyrocou cene prod of i RelaquoI1 dshylu plOltC -dcJiciulbullbull lnrJu YC _ d bco prod1an leYeh or _c 1lIl~
s-ol 01 u _1lIncd buw ppliable ouroc 01 __ ribnlriu ln __~ a bc 01 dloic dI_ plin I+IwoI9) s--nhc~ i ufM rkk ofOcircOldOllioolmiddotbody ~ M do bullbull E Il - plOt pan biiugrave Ite Id1oo 1tc 1idd
nu ilobilil-r vlplOd 11I1 -po Iloo _d _ nd raiIooiq ~ propenico _y Il fNllCl CIcircCriItc d _dorrl ln fVIICUw diccud bull __ Iurion i ~a plt~1I elliccediliucy 01 oucll)tf Oftc 100 1icircIlnria (10_10 nrion) MW bc d nriollQ dcoftd lI5lUllod rc )
Condu_ne and penpecthon Eltnm uYC _- 101 in indQono pngtlaquolt ludiampriltltu~ cIIc1 nd ial opplia W _ ptOd 0 60 ftooooI rlgtc IIddlriool ploi oioa efltn-- TW lboI 100 ond te m iblc i odily bullbullpndi Il ba arcd tho I bu lOS lIf lin 1 1 bull tldncd CClI will bc ltul 1c nd _ rhe ocirc or _ upreooion dlllllIlu (cc dcTclopfncn of lt11 ud im _ Iopo kor _m) _~I 10- up 01 roltlt dil Ir i n_ lgtlc 10 CC op lib egrave f lhe diWIK _ Ir h llbl orn ned w11 r ond 1laquo11 tlc_ rion IIlc n œy will bol dl d la 00- _errraoom le u i ye b WOegrave p fiosoeOf e opecoftc WO 11y lricdly ocu deyclo 01 pooteOcircII Ocircllamp lItd dicd cYOIulioraquo cil bullbull oilI 10 iIl unitbullbull am Ocircl biocotolpic ilS wicll -ill iolaquoco tllo pp_ 01 bull f_ uraaopbil
MeIiPt1=bullbull eIEtel - _ pH _w mol lIrM ~ _ __ r c dot lI_c_ dclter _ Irillile pl ~ - Ilftd Iblt shyilic -pa io dieu oIMliry _1IW11 toI lllc lIIIluar-~ e-pn _ j lU al _
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Productkln lJl MZ)1Mo1 fovm ~
rlaquoriotuJ 0lt0Yf IMIMI i oIDNA ltII bc very bdprul ra rd lJIla1ion fil _fui euym ll_ebullbull Ile idaI_ of polUUIllIy fulnynICS i Iy lino 09 To uIIIlI _ of lbocirc kMtIt idUlrifMd ca u 10 bc de bullniloblc fol ~ dl u vi b 1yUc ic1IIgtd Iicu_ -~ _ _pu lot the pnocI__ofCIU) ~ cm WJa r pIOdrioa or me biouIY Ile ~ d bJ 1amp biocDuo PIIdIoltdon of dle hile AJ_lIYely die CC eocodi do bionulyst n bc 1 d OcircII iable toool
Eltbbullbull1OPhIt blom_ ptOductIon Spococircolixd cqooip of~ bull Plaquo40i 0 owla fficic_ oIopecific bionllJyst dl1y f Ul~lIilolnddiioniIDrmiddotmod f lloA _ ril 1 bc debullbull1opnr ond 0 ho bc Iddruoed inM Iou- or _ pIoocircl Hl-~ lM- J)looJ ioIl ft bc -cd OcircM lgty 09iI of b alipoougrave Of
br 1 obbullbull dfic rmn~ pnxcd (c fedmiddotIlIICh laquoII cclon 0lt nnllllUll cul ) 1amp tdcli opcucircol biolteoclOlt II bullbull ampu-lih rUl CI d ohu Ilw bc cd r pk tpecific ioIIroio Woo- 1000II 00 lie _Iopcd in rhbullbullulioo ofccloalopbila r pad orpolyJJum d (VGII~IOr opcc1lic req inshy W oIic b bol ore Iorcd he IoiJlb ra dl IIK dIbull-rillic 01 on i_n f lucircdIo 01lfClIIIi ioolcd 1IIdI dl _l~jl
Gene do-d orproducllon At p the fc _ pod n II101Y fol lMOIlpIlocirclu _ -ocirc6cltcd rd pmshyltcdn nurdon mil oI kinamp 1 Wy1aoOUcirctlbler rhnc laquoCII IIlO bc deyckI9cd ThbullbullI_inl rid bull i_ r lIIgtlidinJ amplt in pa1Ic ~ CI le n UMllve olmOlive lIlodlOltl- A jIlr ion or h carymbullbull ILo h r dl y 1 indalolppl _ 0 _ don Edma roJi uptaUgraveltgta -rou- ( T blc Z bull 1ll1I+lB
Gribullbullool for 1 (01i1 ete Wl 11 docw opcd nlt y p1aid vctt1HI iDd illk lue Pp ocircbic 1 c p dllCUcircMl 0( ilegrave nq rili d dlc ctllodaoaN01_ E _ bullbullbull folio ta Il~1~ H dl middotenmiddotk_n pbc__ 01 im_-locidy r_lion by 0 middot d p_oocirc i t nli 1lt1 fOlCC h ra oI i iflaUil_Ir M 11 _bco of ugravew nllbk ncludil bonc101 11 111 dI ~a ~ ~h
---_ Md eka o ~ - cJiM ond Hu The objccti of ~ uld le po i id in Iectiloamp lie Ic buM YIII P pl ir dlbullbullnq- cd ampIlltOIyl-oon farltiolopol ocri dl Icotiod of a bo ri1 Il oold bol votdcd wh ro dl poodllttion or u llllla cn_ 0 IKioI bulldo Btit bull likcty 10 le Ie A dJlWIJoclt or- ampnIIlu-loucd - bullbullIe dri~11 hb Iel r odn p_ _ dl Il lIioll or ludo d ion 01 toshyrocou cene prod of i RelaquoI1 dshylu plOltC -dcJiciulbullbull lnrJu YC _ d bco prod1an leYeh or _c 1lIl~
s-ol 01 u _1lIncd buw ppliable ouroc 01 __ ribnlriu ln __~ a bc 01 dloic dI_ plin I+IwoI9) s--nhc~ i ufM rkk ofOcircOldOllioolmiddotbody ~ M do bullbull E Il - plOt pan biiugrave Ite Id1oo 1tc 1idd
nu ilobilil-r vlplOd 11I1 -po Iloo _d _ nd raiIooiq ~ propenico _y Il fNllCl CIcircCriItc d _dorrl ln fVIICUw diccud bull __ Iurion i ~a plt~1I elliccediliucy 01 oucll)tf Oftc 100 1icircIlnria (10_10 nrion) MW bc d nriollQ dcoftd lI5lUllod rc )
Condu_ne and penpecthon Eltnm uYC _- 101 in indQono pngtlaquolt ludiampriltltu~ cIIc1 nd ial opplia W _ ptOd 0 60 ftooooI rlgtc IIddlriool ploi oioa efltn-- TW lboI 100 ond te m iblc i odily bullbullpndi Il ba arcd tho I bu lOS lIf lin 1 1 bull tldncd CClI will bc ltul 1c nd _ rhe ocirc or _ upreooion dlllllIlu (cc dcTclopfncn of lt11 ud im _ Iopo kor _m) _~I 10- up 01 roltlt dil Ir i n_ lgtlc 10 CC op lib egrave f lhe diWIK _ Ir h llbl orn ned w11 r ond 1laquo11 tlc_ rion IIlc n œy will bol dl d la 00- _errraoom le u i ye b WOegrave p fiosoeOf e opecoftc WO 11y lricdly ocu deyclo 01 pooteOcircII Ocircllamp lItd dicd cYOIulioraquo cil bullbull oilI 10 iIl unitbullbull am Ocircl biocotolpic ilS wicll -ill iolaquoco tllo pp_ 01 bull f_ uraaopbil
rlaquoriotuJ 0lt0Yf IMIMI i oIDNA ltII bc very bdprul ra rd lJIla1ion fil _fui euym ll_ebullbull Ile idaI_ of polUUIllIy fulnynICS i Iy lino 09 To uIIIlI _ of lbocirc kMtIt idUlrifMd ca u 10 bc de bullniloblc fol ~ dl u vi b 1yUc ic1IIgtd Iicu_ -~ _ _pu lot the pnocI__ofCIU) ~ cm WJa r pIOdrioa or me biouIY Ile ~ d bJ 1amp biocDuo PIIdIoltdon of dle hile AJ_lIYely die CC eocodi do bionulyst n bc 1 d OcircII iable toool
Eltbbullbull1OPhIt blom_ ptOductIon Spococircolixd cqooip of~ bull Plaquo40i 0 owla fficic_ oIopecific bionllJyst dl1y f Ul~lIilolnddiioniIDrmiddotmod f lloA _ ril 1 bc debullbull1opnr ond 0 ho bc Iddruoed inM Iou- or _ pIoocircl Hl-~ lM- J)looJ ioIl ft bc -cd OcircM lgty 09iI of b alipoougrave Of
br 1 obbullbull dfic rmn~ pnxcd (c fedmiddotIlIICh laquoII cclon 0lt nnllllUll cul ) 1amp tdcli opcucircol biolteoclOlt II bullbull ampu-lih rUl CI d ohu Ilw bc cd r pk tpecific ioIIroio Woo- 1000II 00 lie _Iopcd in rhbullbullulioo ofccloalopbila r pad orpolyJJum d (VGII~IOr opcc1lic req inshy W oIic b bol ore Iorcd he IoiJlb ra dl IIK dIbull-rillic 01 on i_n f lucircdIo 01lfClIIIi ioolcd 1IIdI dl _l~jl
Gene do-d orproducllon At p the fc _ pod n II101Y fol lMOIlpIlocirclu _ -ocirc6cltcd rd pmshyltcdn nurdon mil oI kinamp 1 Wy1aoOUcirctlbler rhnc laquoCII IIlO bc deyckI9cd ThbullbullI_inl rid bull i_ r lIIgtlidinJ amplt in pa1Ic ~ CI le n UMllve olmOlive lIlodlOltl- A jIlr ion or h carymbullbull ILo h r dl y 1 indalolppl _ 0 _ don Edma roJi uptaUgraveltgta -rou- ( T blc Z bull 1ll1I+lB
Gribullbullool for 1 (01i1 ete Wl 11 docw opcd nlt y p1aid vctt1HI iDd illk lue Pp ocircbic 1 c p dllCUcircMl 0( ilegrave nq rili d dlc ctllodaoaN01_ E _ bullbullbull folio ta Il~1~ H dl middotenmiddotk_n pbc__ 01 im_-locidy r_lion by 0 middot d p_oocirc i t nli 1lt1 fOlCC h ra oI i iflaUil_Ir M 11 _bco of ugravew nllbk ncludil bonc101 11 111 dI ~a ~ ~h
---_ Md eka o ~ - cJiM ond Hu The objccti of ~ uld le po i id in Iectiloamp lie Ic buM YIII P pl ir dlbullbullnq- cd ampIlltOIyl-oon farltiolopol ocri dl Icotiod of a bo ri1 Il oold bol votdcd wh ro dl poodllttion or u llllla cn_ 0 IKioI bulldo Btit bull likcty 10 le Ie A dJlWIJoclt or- ampnIIlu-loucd - bullbullIe dri~11 hb Iel r odn p_ _ dl Il lIioll or ludo d ion 01 toshyrocou cene prod of i RelaquoI1 dshylu plOltC -dcJiciulbullbull lnrJu YC _ d bco prod1an leYeh or _c 1lIl~
s-ol 01 u _1lIncd buw ppliable ouroc 01 __ ribnlriu ln __~ a bc 01 dloic dI_ plin I+IwoI9) s--nhc~ i ufM rkk ofOcircOldOllioolmiddotbody ~ M do bullbull E Il - plOt pan biiugrave Ite Id1oo 1tc 1idd
nu ilobilil-r vlplOd 11I1 -po Iloo _d _ nd raiIooiq ~ propenico _y Il fNllCl CIcircCriItc d _dorrl ln fVIICUw diccud bull __ Iurion i ~a plt~1I elliccediliucy 01 oucll)tf Oftc 100 1icircIlnria (10_10 nrion) MW bc d nriollQ dcoftd lI5lUllod rc )
Condu_ne and penpecthon Eltnm uYC _- 101 in indQono pngtlaquolt ludiampriltltu~ cIIc1 nd ial opplia W _ ptOd 0 60 ftooooI rlgtc IIddlriool ploi oioa efltn-- TW lboI 100 ond te m iblc i odily bullbullpndi Il ba arcd tho I bu lOS lIf lin 1 1 bull tldncd CClI will bc ltul 1c nd _ rhe ocirc or _ upreooion dlllllIlu (cc dcTclopfncn of lt11 ud im _ Iopo kor _m) _~I 10- up 01 roltlt dil Ir i n_ lgtlc 10 CC op lib egrave f lhe diWIK _ Ir h llbl orn ned w11 r ond 1laquo11 tlc_ rion IIlc n œy will bol dl d la 00- _errraoom le u i ye b WOegrave p fiosoeOf e opecoftc WO 11y lricdly ocu deyclo 01 pooteOcircII Ocircllamp lItd dicd cYOIulioraquo cil bullbull oilI 10 iIl unitbullbull am Ocircl biocotolpic ilS wicll -ill iolaquoco tllo pp_ 01 bull f_ uraaopbil