Exploring ICP, Tissue Oxygenation and RSNA with Implantable Telemetry Sponsored by: Prof. Jacqueline Phillips Professor of Neuroscience Faculty of Medicine and Health Sciences Macquarie University Sydney NSW Australia Dr. Fiona McBryde Research Fellow, Department of Physiology University of Auckland New Zealand
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Exploring ICP, Tissue Oxygenation and RSNA with Implantable Telemetry
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Exploring ICP, Tissue Oxygenation and RSNA with Implantable Telemetry
Sponsored by:
Prof. Jacqueline Phillips
Professor of Neuroscience
Faculty of Medicine and Health Sciences
Macquarie University
Sydney NSW Australia
Dr. Fiona McBryde
Research Fellow,
Department of Physiology
University of Auckland
New Zealand
InsideScientific is an online educational environment designed for life science researchers. Our goal is to aid in
the sharing and distribution of scientific information regarding innovative technologies, protocols, research tools
Copyright 2015 S. Lau, Millar & InsideScientific. All Rights Reserved.
1. Millar Telemetry System
2. Cohousing feature (New!)
An implantable telemetry system with a difference!
Millar Telemetry System
1. Telemeters – fully implantable,
rechargeable (see www.millar.com)
2. SmartPad – wireless charger and
signal receiver
3. Configurator System –pairs telemeters and SmartPads(30 Channels), enable/disable cohousing
House 2 rats in one cage (>175g each)
Implant 2 telemeters in one large rat (>350g)
NC3Rs on telemetry…“investigators should be aware of potential sources of pain and distress within telemetry projects, including: distress induced by housing animals individually” http://3rs.ccac.ca/en/research/reduction/telemetry.html
Cohousing – reduce costs and increase research opportunity
Primary (1°) SmartPad
• Collects data from telemeter 1 only
• Monitors battery and charges two implanted telemeters
How Cohousing Works
Secondary (2°) SmartPad
• Collects data from telemeter 2 only
• Charging disabled
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1 2
High Quality Data Recordings
Same high quality data expected from a Millar Telemetry System…
Just more of it!
Cohousing helps address ethical concerns around social isolation
Reduces housing costs
Same high quality data expected from a Millar Telemetry System!
Summary
For more information, visit www.millar.comor contact us on [email protected]
In the lab, data gets you results. Innovation gets you noticed.
The Millar Telemetry System adds a higher level of understanding to any cardiovascular, neuroscience, toxicology or pharmacology study — bringing the accuracy and precision you expect from Millar in a fully implantable device.
• Smartpads need to be specially configured -- important to consult with Millar
• TWO smartpads per rat – one active charge field.
• Second (field off) smartpadcan be off to one side.
Housing Dual Telemeter Animals:
Opening new channels for data collection:
Simultaneous recordings of multiple inputs –allows real-time calculation of respiration rate and cerebral perfusion pressure
Other possibilities: BP, ICP and Renal SNA
Possible to combine any two telemeter types, to access a wide range of signals
• Demanding surgical set-up for both experimenter and rat
• Aseptic technique and good post-surgical care essential
• Longer recovery time (>5-7 days)
• Access to multiple signals – unique insight
• Further resources:
Millar Knowledge Centre (www.millar.com)
Papers
Collaborations
Tips, Pointers and Summary
Direct conscious telemetry recordings demonstrate increased renal sympathetic nerve activity in rats with chronic kidney disease
Professor Jacqueline Phillips
Professor of Neuroscience
Faculty of Medicine and Health Sciences
Macquarie University
Sydney NSW Australia
1. Experimental context
2. Why telemetry?
• Advantages and challenges
• Study outcomes
3. What’s next?
Conscious recording of renal sympathetic nerve activity in an animal model of disease
Salman et. al 2015 – Frontiers in Physiology
Direct conscious telemetry recordings demonstrate increased
renal sympathetic nerve activity in rats with chronic kidney disease
Chronic kidney disease (CKD) is associated with sympathetic hyperactivity and impaired blood pressure control reflex responses, yet direct evidence demonstrating these features of autonomic dysfunction in conscious animals is still lacking. Here we measured renal sympathetic nerve activity (RSNA) and mean arterial pressure (MAP) using telemetry-based recordings in a rat model of CKD, the Lewis Polycystic Kidney (LPK) rat, and assessed responses to chemoreflex activation and acute stress.
Ibrahim M. Salman, Divya Sarma Kandukuri, Joanne L. Harrison, Cara M. Hildreth and Jacqueline K. Phillips*