Experimental Acute Myocardial Infarction (AMI)

CARDIAC ISCHAEMIA

&STEM CELLS

by/ M.Mohsen.ElAlfy M.Fathi

Ischaemic Heart Disease AMI

Def. ?

Ischaemic Heart Disease

AMI13%

12%

6%

5%3%

3%3%3%2%2%

49%

Ischaemic Heart DiseaseStrokeCOPDLower Respiratory InfectionTrachea, Bronchi & Lung cancersHIV/AIDSDiarrhoeal DiseasesDiabetes MellitusRoad InjuryHypertensive Heart DiseaseOthers

Stem Cells

Acute Phase(Inflam

.)1-7 d

Sub-Acute Phase

(Granulation)1-3 wk

Chronic Phase(Scar)1 m

Patho-pysiology of Myocardial Infarction

ModelsSurgica

lLAD

Ligation

Medical IDoxorubicin

Medical II

Isoproterenol

Mechanism

SurgicalLAD Ligation

SimulationEffectivenessReliabilitySurvival

Medical IDoxorubicin

SimulationEffectivenessReliabilitySurvivalO H

Medical IIIsoproterenol

SimulationEffectivenessReliabilitySurvival

O Ca+2

O

ATPO H

Investigations

• Macroscopic: Nitro-blue Tetrazolium

• Microscopic: H&E and Mallory’s Tri-chrome

Macroscopic & Microscopic

• CBC• Kidney functions: urea and

creatinine• Liver enzymes: SGOT and SGPT

Routine lab. Work

• Troponin: TI isozyme• Creatin Phospho-kinase: CPK-MB

isozyme• Lactate Dehydrogenase: LDH Total

Cardiac Enzymes

• Glutathione: GSH Total• Super-oxide Dismutase: SOD Markers of

Oxidative Stress

???Questions

Thank You