Exercise physiology. 1) Wilmore, J. H., & Costill, D. L. (1994). Physiology of sport and exercise. Champaign, IL: Human Kinetics. 2) Åstrand, P.-O., Rodahl,

Exercise physiology

Exercise physiology

1) Wilmore, J. H., & Costill, D. L. (1994). Physiology of sport and exercise. Champaign, IL: Human Kinetics.

2) Åstrand, P.-O., Rodahl, K., Dahl. H. A., & Strømme, S. B. (2003). Textbook of Work Physiology: Physiological Bases of Exercise (4th ed.). Champaign, IL: Human Kinetics.

3) Brooks, G. A., Fahey, T. D., & White, T. P. (1995). Exercise physiology: human bioenergetics and its applications (2nd ed.).

4) Mountain View, CA: Mayfield Publishing Company. Sharkey, B. J. (1990). Physiology of fitness. Champaign, IL: Human Kinetics.

Recommended literature:

Exercise => causes the changes in human body

A) Acute response to one bout of exercise – e.g. ↑ heart rate (HR), ↑ body temperature (HR)B) Chronic adaptation to repeated bouts of exercise

- e.g. ↓ HR at rest and ↓ HR at exercise (same intensity)

Změny vybraných parametrů

Exercise

Muscle activity requires energy. During exercise are energy demands enhanced.

- decrease of ATP, increase of ADP

=> causes the changes in human body

A) Acute response to one bout of exercise – e.g. ↑ heart rate (HR), ↑ body temperature (HR)B) Chronic adaptation to repeated bouts of exercise

- e.g. ↓ HR at rest and ↓ HR at exercise (same intensity)

Muscle contractile work = transforming chemical energy into kinetic (mechanical) energy

Energy metabolism

ATP

B) Catabolism – release of energy (glycolysis, lipolysis)

A) Anabolism- creation of reserve (carbohydrate, fat, proteins)

hydrolisisADP + P + E

ATP – adenosine thriphosphate- common energy “currency”

ADP – adenosine diphosphateP - phosphateE - energy (e.g. for muscle contraction)

phosphorylation

Energy metabolism Energy sources

1] Polysaccharides simple sugars glucose (glycogen)

2] Fats (triglycerides) fatty acids (FFT) and glycerol

3] Proteins amino acids

Energy metabolism

G

cell plasmaplasma membrane blood

G Glycogen (GG)G – 6 - P

Glucose is the only one that can be broken down anaerobically and aerobically as well.

Anaerobic glycolysis

pyruvic acid

2 ATP (G)3 ATP (GG)

lactic acid

Energy metabolism

pyruvic acid (pyruvate)

Aerobic glycolysis

cell plasma

mitochondrial membrane

Acetyl CoAmitochondrion

Citric acid cycle

NADH (nicotinamide adenine dinucleotid) and FADH

CO2

Energy metabolism

NADH + O2 + 3ADP 3ATP + NAD + H2O

oxidative phosphorylation – in mytochondrion (electron transport chain)

1 NADH=3 ATP

FADH + O2 + 2ADP 2ATP + FAD + H2O

1 FADH=2 ATP

Energy metabolism

Aerobic glycolysis 36 ATP 36 ATP

Anaerobic glycolysis 2 ATP 3 ATP

From one molecule G GG

Total glycolysis 38 ATP 39 ATP

Glycogen reserves are in muscle cells (500 g) and in liver (100 g).- From 1.500 to 2.500 kcal.

1 calorie (cal) is the amount of the energy increases the temperature of 1 gram H2O from 14.5ºC to 15.5ºC.

Energy metabolism Fat - triglyceride = FFA (free fat acids) + glycerol in subcutaneous tissue

(141 000 kcal).

Adipose tissuereduction

triglyceride FFA + Glycerol Hormone-sensitive lipase

Glucose metabolism

Beta oxidation

Acetyl CoA

Citric acid cycle

NADH and FADH

CO2

NADHand FADH

Energy metabolism

Acetyl CoA

Citric acid cycle

Glucose

FFA proteins

and/or

anaerobicaerobic

lactic acid

plasma membrane

NADH and FADH

Electron transport chain

pyruvate

Energy metabolism Anaerobic metabolism

- only carbohydrate- increases when lack of O2 and not enough time- lower amount of ATP, but very fast and huge in short time- production of lactic acid

Anaerobic metabolism

- carbohydrate, fats, proteins- enough of O2

- higher amount of ATP, but slower

Note: proteins are not very important sources of energy (5-10%). Amino acids are preferabely used as a building matters for muscles, hormones, etc.

Energy metabolism

ATPhydrolisis

ADP + P + Ephosphorylation

ATP is only the one immediate source of energy for muscles work, etc.

Other ways of the creation (phosporylation):

ATP + C ADP + CP(creatine phosphate)

ATP + AMP ADP + ADP

Exercise

intensity (type)

Duration Dominative source of

energy

Production

of lactic

muscle

fiber type

maximum till 15 s ATP, CP Small FG

submaximal 15 – 50 s ATP, CP, anaerobic

glycolysis

Maximum FG

and FGO

Short term

endurance

till 120 s Anaerobic and aerobic

glycolysis

Submax. FG

and FGO

Middle endurance till 10 min aerobic glycolysis medium FGO, SO

Long term

endurance

More than

10 min

aerobic glycolysis,

latter lipolysis

small SO

Zones of energy supply

Anaerobic free of lactic acid

Anaerobic with lactic acid

Aerobic free of lactic acid

- s trváním pokles

(?Havlíčková et al, 1991)

Total energy expenditure

Dominant way of restoration of ATP is oxidative phosphorylation

Acute reaction of the body (neurohumoral controlled) for increase

in supply of working muscles by energy sources and O2

- increase glucose in blood (from liver glycogen)- activation of FFA (activation of hormone sensitive lipase)

Mechanism of energy release in dependence on intensity

aerobic anaerobic

NOTE:Ideal model

REST

Anaerobic threshold

VO2max

Aerobic threshold

Sources of energy by increasing exercise intensity

RQ fats = 0,7

RQ carbohydrates = 1

1 g = 9,3 kcal

1 g = 4,1 kcalRQ =

CO2

O2

(Hamar & Lipková, 2001)

fats

glucose

glycogen

exercise intensity % VO2max

energy expenditure kJ/min

Sources of energy by increasing exercise intensity

RQ =CO2

O2

CO2 - expired

O2 - inspired

RQ – respiration quotient – ratio between CO2 and O2

RQ carbohydrates = 1 = 1 l CO2/1 l O2

RQ fats = 0,7 = 0.7 l CO2/1 l O2

RQ normal (mixed) = 0,82

more O2

Lipids (FFA)- more energy (1 g = 9,3 kcal)- need more O2 (EE = 4,55 kcal)- use while enough of O2 (at rest, low intensity of exercise)

Lipids (FFA)- more energy (1 g = 9,3 kcal)- need more O2 (EE = 4,55 kcal)- use while enough of O2 (at rest, low intensity of exercise)

– energetic equivalent– shows amount of energy released while applied 1 liter of O2 on carbohydrate or on FFA

EE

Lipids (FFA)- more energy (1 g = 9,3 kcal)- need more O2 (EE = 4,55 kcal)- use while enough of O2 (at rest, low intensity of exercise)

Carbohydrates- less energy (1 g = 4,1 kcal)

- need less O2 (EE = 5,05 kcal)

- use while not enough of O2 (higher intensity, and anaerobically as well)

- small amount is always use at rest

Sources of energy by increasing exercise intensity

RQ lipids = 0,7

RQ carbohydrates = 1

1 g = 9,3 kcal

1 g = 4,1 kcalRQ =

CO2

O2

(Hamar & Lipková, 2001)

fats

glucose

glycogen

exercise intensity % VO2max

energy expenditure kJ/min

Wasserman scheme of transport O2 a CO2

O2

CO2

(Wasserman, 1999)

Muscle work

Transport O2 and CO2

Ventilation

AIRMito-chon-drion

cardiovascular s. lungsmuscles

The more O2 is delivered to working muscle, the higher aerobic production of energy (ATP)

Better endurance performance, smaller production of lactic acid while the same speed of run, longer lasting exercise, etc.

Wasserman scheme of transport O2 a CO2

O2

CO2

(Wasserman, 1999)

Muscle work

Transport O2 and CO2

Ventilation

AIRMito-chon-drion

cardiovascular s. lungsmuscles

Fick equation:

VO2 = Q × a-vO2

VO2 – oxygen consumption [ml/min]

Q – cardiac output [ml/min]

a-vO2 – arteriovenous oxygen difference

SV HR

SV – stroke volume [ml]

HR – heart rate [beet/min]

×

a-vO2 – arteriovenous oxygen difference

DA-V – arteriovenous oxygen difference

- difference in the oxygen content of arterial and mixed venous blood- the value tells about the amount of oxygen used by working muscles- depends on the muscle ability to absorb and use the O2 from blood (perfusion, amount of capillary, mitochondrion, number of working muscles, etc.)

(100 ml krve is saturated by 20 ml O2)

- at rest 50 ml O2 from 1 L of blood

- during exercise 150-170 ml O2 1 L of blood

(1 L of blood is saturated by 200 ml O2)

1 L of blood is saturated by 200 ml O2

To ensure during exercise:

↑BF (breathing frequency, rate)

- from 12-16 breath/min up 60 (70 and more)

↑TV (tidal volume)- from 0.5 L up 3 L

Minute ventilation (VE) = BF × TV

- at rest 6 L/min = 12 × 0.5

- during maximal

exercise 180 L/min = 60 × 3

VO2 = Q × DA-V

.

Q = HR × SV

4,9 L = 70 beat/min × 70 mlrest: SEDENTARY

4,9 L = 40 beat/min × 120 mlrest: TRAINED

In work: increase of HR and SV - ↑ Q

- SV increases till HR 110–120 beet/min (from 180 beet/min decreases)- HRmax = 220 - age

VO2 = Q × DA-V

.

human (70kg): 245 : 70 = 3,5 ml O2/kg/min (1MET)

rest: VO2 = 4,9 L of blood × 50 ml O2

VO2 = 245 ml/min

Q = HR × TV

4,9 L = 70 beat/min × 70 mlrest: SEDENTARY

4,9 L = 40 beat/min × 120 mlrest: TRAINED

VO2 = Q × DA-V

.

Q = SF × SV

20 L = 200 beat/min × 120 mlMax. exercise: SEDENTARY

35 L = 200 beat/min × 175 mlMax. exercise: TRAINED

VO2 = Q × DA-V

.

70 kg human:3140 : 70 = 45 ml O2/kg/min (13 METs)

Max. exercise:

VO2max= 20 L of blood × 157 ml O2

VO2 max= 3140 ml/min

SEDENTARY:

VO2 = Q × DA-V

.

70 kg human:5950 : 70 = 85 ml O2/kg/min (25 METs)

VO2max= 35 L of blood × 170 ml O2

VO2 max= 5950 ml/min

TRAINED:

Max. exercise:

VO2max - is maximum volume of oxygen that by the body can consume during intense (maximum), whole body exercise.

- expressed:- in L/min- in ml/kg/min- METs

1 MET - resting O2 consumption (3.5 ml/kg/min)

10 METs = 35 ml/kg/min

Definition and explanation of VO2max

20 METs = 70 ml/kg/min

Higher intensity of exercise

Higher energy demands (ATP)

Increase in oxygen consumption

Lower VO2max = less energy = worse achievement

Importance of VO2max

Importance of VO2max

The more is O2 supplied to working muscles, the more higher is an amount of aerobically

produced energy.It means higher speed of running, latest

manifestation of fatigue, etc.

During endurance activity is being ATP resynthesized mainly aerobically from lipids

and carbohydrates.

It shows the capacity for aerobic energy transfer.

Average values of VO2max

Average (20/30 years) not trained:

- female 35 ml/kg/min

- male 45 ml/kg/min

Trained: to 85 ml/kg/min (cross-country skiing)

Decreases with age. Lower in female.

Average values of VO2max

Limitation factors of VO2max

O2

CO2

(Wasserman, 1999)

Muscle work

Transport O2 and CO2

Ventilation

AIRcardiovascular s. lungsmuscles

Limitation factors of VO2max

1) Lungs – no limitation factor

2) Muscles – is limitation factor

3) Cardiovascular system – dominant limitation factor

Wasserman scheme of transport O2 a CO2

O2

CO2

(Wasserman, 1999)

Muscle work

Transport O2 and CO2

Ventilation

AIRMito-chon-drion

cardiovascular s. lungsmuscles

On increase of VO2max participate:

1) Increase of DA-Vmax – shares on increase about 20%

2) Increase of Qmax – shares aboout 70 - 85%

VO2max = Qmax × DA-Vmax

Influence of the gender, health condition, age

Heredity – the increase of VO2max by training only to max. 25%

Gender – in female lower muscle mass, lover hemoglobin

Age – decrease of active body mass, activity of enzymes…

Sources of energy by increasing exercise intensity

RQ lipids = 0,7

RQ carbohydrates = 1

1 g = 9,3 kcal

1 g = 4,1 kcalRQ =

CO2

O2

(Hamar & Lipková, 2001)

fats

glucose

glycogen

exercise intensity % VO2max

energy expenditure kJ/min

3,5

VO2max[ml/kg/min]

45

exercise intensity (speed, load, etc.)

AT50-60% VO2max

AT (aerobic threshold)

- exercise intensity, when „exclusive“ aerobic covering ends.

- exercise intensity, from which anaerobic covering starts and lactate is being produce

- level of lactate: 2 mmol/L of blood

3,5

VO2max[ml/kg/min]

45

AT50-60 % VO2max

AnT70-90 % VO2max

plateau

exercise intensity (speed, load, etc.)

AnT (anaerobic threshold)

- exercise intensity, when anaerobic covering exceed aerobic.

- exercise intensity, when dynamic balance between production and breakdown of lactate is disturbed

- level of lactate: 4 mmol/L of blood and is increasing (onset of blood lactate accumulation).

- at about approximately 8 mmol/L o blood is impossible to continue in exercise (trained even 30 mmol/L of blood)

AnT (anaerobic threshold)

- can be estimate from VO2max:

AnT = VO2max/3,5 + 60

AnT = 35/3,5 + 60

AnT = 70 %VO2max

60 % of VO2max - AT1 MET

3,5

VO2max[ml/kg/min]

45

AT50-60 % VO2max

AnT70-90 % VO2max

exercise intensity (speed, load, etc.)

3,5

VO2max[ml/kg/min]

45

AT50-60 % VO2max

AnT70-90 % VO2max

lactate energy sources

? 1,1 mmol/L

fiber type

2 mmol/L

4 mmol/L

fat > sugar

fat = sugar

fat < sugar

I.

I., II. a

I., II. a, II. b

L is oxidized (heart ,not working muscles)

onset of lactate accumulation – ↑ pH

exercise intensity (speed, load, etc.)

(Hamar & Lipková, 2001)

Exercise intensity during endurance activity (>30 minutes) can not be above AnT.

1) Before start of exercise- increase in O2 consumption (emotions, reflexions)

2) Initial phase of exercise (till 5 minutes)

- rapid increase in the oxygen consumption

3) Steady state- balance between the energy required by working muscles and the rate of ATP produced by aerobic metabolism- O2 is almost constant- lactate level is constant - HR is in the range ±4 beats (real steady state)

Time [min]

VO2max[ml/kg/min]

AnT

0 5 30

3.5

before start initial phase steady state

O2 deficit

• Oxygen deficit

- Insufficient supply of working muscles with O2, at the beginning of exercise (slower ↑ SF and SV, BF and TV).

- disbalance between O2 demands and supply leads to use of anaerobic metabolism – production of LACTATE ( ↑ H+ – metabolic acidosis – death point).

- when O2 demands ensured – second breath

- after termination of exercise the increased O2 consumption persists = oxygen debt

Time [min]

VO2max[ml/kg/min]

AnT

0 5 30

3.5

before start initial phase steady state

O2 deficit

O2 debt

Oxygen debt- synthesis of ATP and CP- resynthesis of lactate (back to glycogen in the liver, and oxidation by muscles and myocardium)

- acceleration of release of lactate from muscles and better blood perfusion of muscles resynthesising lactate, is possible by low intensive exercise: (till 50 % VO2max – below AT)

- recovery of myoglobin, hemoglobin, hormone, etc.- the major part (till 30 min), mild oxygen debt can persist 12-24 hours.

Time [min]

VO2max[ml/kg/min]

AnT

0 5 25

3.5

false steady state- above AnT

major O2 debt

before start initial phase steady state

Time [min]

VO2max[ml/kg/min]

AnT

0 2 30

3.5

AP

smaller O2 debt

before start initial phase steady state

(Hamar & Lipková, 2001)

oxygen consumption (L/min)

rest exercise

time (min)

sedentary - steady state is reached latter

trained - steady state is reached earlier

Practical importance of VO2max

male A female

VO2max = 70ml/kg/min

VO2max = 35 ml/kg/min

AnP = VO2max/3,5 + 60

80%

70%

male A male B

VO2max = 70ml/kg/min VO2max = 70 ml/kg/min

80%90%

Practical importance of VO2max

Critical parameter of endurance abilities is not VO2max,

but AnT.

However VO2max is conditional parameter of AnT.