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Evolution of Autoclave use at the Sanger Institute Jon Lovell 23 rd October 2014.

Dec 15, 2015

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Page 2: Evolution of Autoclave use at the Sanger Institute Jon Lovell 23 rd October 2014.

Evolution of Autoclave use at the Sanger Institute

Jon Lovell23rd October 2014

Page 3: Evolution of Autoclave use at the Sanger Institute Jon Lovell 23 rd October 2014.

2004

Media Team– 1 x 400L Autocalve

Glassware– 1 x 600L Autoclave

Waste– 1 x 600L Autoclave

Page 4: Evolution of Autoclave use at the Sanger Institute Jon Lovell 23 rd October 2014.

2005 Containment Laboratories– 1 x 250L Autoclave (Integrated Boiler)– 3 x 50L Autoclave (Self generating)– 5 x 10L Benchtop Autoclaves

Page 5: Evolution of Autoclave use at the Sanger Institute Jon Lovell 23 rd October 2014.

2006Research Support Facility– 4 x 6,000L Autoclave (Central

steam supply)– 1 x 550L Autoclave (Central

steam supply)– 2 x 50L Benchtop Autoclave

Page 6: Evolution of Autoclave use at the Sanger Institute Jon Lovell 23 rd October 2014.

2005-2014Services Teams (Media, Glassware and Waste)

– 5 x 600L Autoclave (Integrated Boiler)– 2 x 250L Autoclave (Integrated Boiler)

Page 7: Evolution of Autoclave use at the Sanger Institute Jon Lovell 23 rd October 2014.

Choosing an autoclave

– Size– Available space– Contingencies

– Useage– Dry Goods– Liquids– Fabrics– Waste (Nature of waste)

– Maintenance– Third party support

– Ergonomics

Page 8: Evolution of Autoclave use at the Sanger Institute Jon Lovell 23 rd October 2014.

Waste ContainersAluminium Bins

Pros:• Faster cycle time• Not too heavy• Secure for transport

Cons:• Fragility due to

compromise on weight.• Seal maintenance

Page 9: Evolution of Autoclave use at the Sanger Institute Jon Lovell 23 rd October 2014.

Management of Laboratory AutoclavesWhich Standards/Guidance?

– BS2646 Autoclaves for Sterilization in Laboratories– BS3970 Sterilizing and disinfecting equipment for

medical products– BSEN285 Sterilization-Steam Sterilizers-Large

Sterilizers– PM73 Safety at Autoclaves HSE– CFPP01(HTM2010/HTM01) Management and

decontamination of surgical instruments used in acute care

Who is responsible for the safety of your site autoclaves?

Page 10: Evolution of Autoclave use at the Sanger Institute Jon Lovell 23 rd October 2014.

ACIST trainingAdvanced course in sterilization technology

Based around health sector requirements

Course covers:• Standards and guidance• Microbiology• Validation• Steam sterilization• Washer disinfectors• Other sterilization and decontamination

techniques• Role of AE(D)