OEB 100 – 11.01.2 Evolution in Action (OEB 100) Instructor: Christopher Marx Teaching fellow: Dipti Nayak Weekly meeting: NW B127 Time: Mondays 4 – 5:30 pm Laboratory: NW 152 Time: 24/7/365 open access Website: http://isites.harvard.edu/k77 185 Please let me out of here: my barnacles and pigeons need me!
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OEB 100 – 11.01.24
Evolution in Action (OEB 100)
Instructor: Christopher MarxTeaching fellow: Dipti Nayak
Weekly meeting: NW B127Time: Mondays 4 – 5:30 pm
Laboratory: NW 152Time: 24/7/365 open access
Website: http://isites.harvard.edu/k77185
Please let me out of here: my
barnacles and pigeons need me!
Integration of systems-level physiology and evolution
genotype
phenotype
environment
physiology
Molecular, cell & developmental
biology, biochem.
Integration of systems-level physiology and evolution
genotype
phenotype
mutation
environment
genotype
phenotype
genotype
phenotype
ecological interactions
selection & drift
Evolutionary biology, ecology
Integration of systems-level physiology and evolution
genotype
phenotype
mutation
environment
genotype
phenotype
genotype
phenotype
ecological interactions
selection & drift
physiology
“Biology”
Use adaptation of laboratory populations of microbes as a forum to explore how biological systems
evolve.
The “functional synthesis”(Dean & Thornton, 2007)
Could tackle all levels through various aspects of the project…
• Genetic mechanisms underlying stress response will be analyzed.
Sub-Projects
Coexistence of different genotypes?
• Genotype 1 : Can withstand high concentrations of Formaldehyde which leads to penalty in growth at lower concentrations.
• Genotype 2 : Cannot withstand high concentrations of Formaldehyde and grows faster at lower concentrations.
Tradeoffs between substrates?
• How has the ability to grow on formaldehyde affected growth on other compounds?
• We know that isolates from one population cannot grow on most carbon compounds it previously could. So there are MASSIVE tradeoffs?
• What are they?
• Can evolving these populations in an alternate environment help these isolates recover?
(Lee et.al. Evolution 2009)
Genomics and Genetics
• Pyrosequencing evolved strains to determine genetic loci that have undergone mutations.
• Examining these loci in replicate populations to check for parallel mutational events.
• Reconstructing mutations in the ancestral background to determine the order of mutational events and the phenotypic change caused by
individual mutations.
• Conducting Microarray/RNA sequencing to understand patterns of global gene expression in evolved and ancestral strains.
Formaldehyde-Induced Mutagenesis?
• Mutations arise spontaneously (one in ~300 cells), but at rates that can be affected by mutations (in DNA repair) or by the environment.
• From genome resequencing we’ll know if there was an elevated mutation rate.
• If so we can determine if formaldehyde specifically induces a higher mutation rate in the original strain, or whether the evolved strain has lowered (or increased) this rate.
From first time in 2009…
Last spring…
Undergraduate Diversity at the Evolution meetings 2011We are pleased to announce a NSF-funded program for bringing talented and diverse undergraduates to the Evolution meetings this June 17-21 in Norman, Oklahoma. For the ninth year in a row we will fly a cohort of 25 undergraduates from throughout the US and Puerto Rico to present a poster at the meetings, receive mentoring from graduate students, postdocs and faculty, and participate in the Diversity Social as well as a career-oriented 'Undergraduate Futures in Evolutionary Biology' panel and discussion. The program covers the costs of travel, registration, food and accommodation at the meetings. The application deadline is 1 March, but admissions will be reviewed as they are received, and so the earlier you apply, the better. Applications are welcomed from all undergraduates, and the admissions goal is to create a diverse pool of students. An overview of the program and student eligibility can be found at:http://www.oeb.harvard.edu/faculty/edwards/community/application.html
Applications consist of a short statement of interest, a letter of recommendation and the title and abstract of the poster to be presented.
In addition, we will be soliciting names of graduate students, postdocs and faculty members who would like to serve as mentors during the meetings. Mentors take pairs of students and attend talks with them, introduce them to colleagues, network and generally make the meetings a welcoming place for them. Although costs are not covered for mentors it is an unusually rewarding experience. Contact Richard Kliman <[email protected]> if you are interested in serving as a mentor.