373 Evaluation of the proliferative effect of clinacanthus nutans glycerine and moringa oleifera seed oil extraction on human gingival fibroblast cell line Sirinthip Choonate, Suwanna Korsuwannawong, Kongthawat Chairatvit Sirinthip Choonate 1 , Suwanna Korsuwannawong 2 , Kongthawat Chairatvit 3 1 B.Sc.(Applied Biology) Department of Oral Biology Faculty of Dentistry, Mahidol University. 2 B.Sc., M.Ed. (Research and Statistics) Research office Faculty of Dentistry, Mahidol University. 3 B.Sc. Ph.D. (Biochemistry) Department of Oral Biology Faculty of Dentistry, Mahidol University. Evaluation of the proliferative effect of clinacanthus nutans glycerine and moringa oleifera seed oil extraction on human gingival fibroblast cell line Correspondence author: Suwanna Korsuwannawong Research office Faculty of Dentistry, Mahidol University 6 Yothi Rd., Rajathevi, Bangkok 10400. Tel: 02-200-7620-24 Fax: 02-200-7622 E-mail: [email protected]Received: 3 February 2014 Accepted: 16 May 2014 Abstract Objective: Clinacanthus nutans glycerine and Moringa oleifera seed oil are commonly used as herbal medicines in Thailand. Previous studies have shown the anti-inflammatory effects and promotion of the wound healing process. However, there is no study for both extracts on oral cells. The aim of this work is; therefore, to study the proliferative effect of both herbal Thai Medicine on Human Gingival Fibroblast (HGF) cell line. Materials and methods: HGF cells were treated with herbal extracts at concentrations of 0.01, 0.1 and 0.5% (V/V). Cytotoxicity was evaluated by MTT assay. Sulforhodamine B (SRB) standard staining method was used to monitor cell proliferation for 10 days after treatment with various concentrations of herbal extracts. Results: Both herbal extracts showed no toxicity to HGF cultures in all tested concentrations. the result of Clinacanthus nutans glycerine and Moringa oleifera seed oil extractions at concentrations of 0.01, 0.1 and 0.5% (V/V) the percentage of cell viability was 102, 105, 99 and 108, 110, 105, repectively. However, both extracts promoted cell proliferation by the concentration- dependent manner. Clinacanthus nutans glycerine showed 117.85%, 125.00% and 130.35% proliferative rates (P<0.05) compared to the control at concentrations of 0.01, 0.1 and 0.5% (V/V), repectively. showed significantly increased cell proliferation compared with the control group (P<0.05). Moringa oleifera seed oil showed a slightly lower proliferative effect on HGF (104.28%, 114.28% and 118.57% at concentrations of 0.01, 0.1 and 0.5% (V/V), repectively) compared to that of Clinacanthus nutans glycerine. Conclusion: The result suggests that these two Thai medicinal plant extracts promote cell proliferation and might be able to use as a potential medicines for wound healing process on periodontal diseases. Key words: Clinacanthus nutans glycerine, HGF cell line, Moringa oleifera seed oil, MTT assay, periodontal diseases, proliferation, wound healing How to cite: Choonate S, Korsuwannawong S, Chairatvit K. Evaluation of the proliferative effect of clinacanthus nutans glycerine and moringa oleifera seed oil extraction on human gingival fibroblast cell line. M Dent J 2014; 34: 373-84. Dental Journal Original Article Mahidol Dental Journal
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373Evaluation of the proliferative effect of clinacanthus nutans glycerine and moringa oleifera seed oil extraction on human gingival fibroblast cell line Sirinthip Choonate, Suwanna Korsuwannawong, Kongthawat Chairatvit
1 B.Sc.(Applied Biology) Department of Oral Biology Faculty of Dentistry, Mahidol University. 2 B.Sc., M.Ed. (Research and Statistics) Research office Faculty of Dentistry, Mahidol University. 3 B.Sc. Ph.D. (Biochemistry) Department of Oral Biology Faculty of Dentistry, Mahidol University.
Evaluation of the proliferative effect of clinacanthus nutans glycerine and moringa oleifera seed oil extraction on human gingival fibroblast cell line
Correspondence author: Suwanna Korsuwannawong Research office Faculty of Dentistry, Mahidol University 6 Yothi Rd., Rajathevi, Bangkok 10400. Tel: 02-200-7620-24 Fax: 02-200-7622 E-mail: [email protected] Received: 3 February 2014 Accepted: 16 May 2014
Abstract Objective: Clinacanthus nutans glycerine and Moringa oleifera seed oil are commonly used as herbal medicines in Thailand. Previous studies have shown the anti-inflammatory effects and promotion of the wound healing process. However, there is no study for both extracts on oral cells. The aim of this work is; therefore, to study the proliferative effect of both herbal Thai Medicine on Human Gingival Fibroblast (HGF) cell line.
Materials and methods: HGF cells were treated with herbal extracts at concentrations of 0.01, 0.1 and 0.5% (V/V). Cytotoxicity was evaluated by MTT assay. Sulforhodamine B (SRB) standard staining method was used to monitor cell proliferation for 10 days after treatment with various concentrations of herbal extracts.
Results: Both herbal extracts showed no toxicity to HGF cultures in all tested concentrations. the result of Clinacanthus nutans glycerine and Moringa oleifera seed oil extractions at concentrations of 0.01, 0.1 and 0.5% (V/V) the percentage of cell viability was 102, 105, 99 and 108, 110, 105, repectively. However, both extracts promoted cell proliferation by the concentration- dependent manner. Clinacanthus nutans glycerine showed 117.85%, 125.00% and 130.35% proliferative rates (P<0.05) compared to the control at concentrations of 0.01, 0.1 and 0.5% (V/V), repectively. showed significantly increased cell proliferation compared with the control group (P<0.05). Moringa oleifera seed oil showed a slightly lower proliferative effect on HGF (104.28%, 114.28% and 118.57% at concentrations of 0.01, 0.1 and 0.5% (V/V), repectively) compared to that of Clinacanthus nutans glycerine.
Conclusion: The result suggests that these two Thai medicinal plant extracts promote cell proliferation and might be able to use as a potential medicines for wound healing process on periodontal diseases.
How to cite: Choonate S, Korsuwannawong S, Chairatvit K. Evaluation of the proliferative effect of clinacanthus nutans glycerine and moringa oleifera seed oil extraction on human gingival fibroblast cell line. M Dent J 2014; 34: 373-84.
Dental Journal Original ArticleMahidol Dental Journal
374 Evaluation of the proliferative effect of clinacanthus nutans glycerine and moringa oleifera seed oil extraction on human gingival fibroblast cell line Sirinthip Choonate, Suwanna Korsuwannawong, Kongthawat Chairatvit
Dental Journal Original Articleวิทยาสารทันตแพทยศาสตรมหิดล
375Evaluation of the proliferative effect of clinacanthus nutans glycerine and moringa oleifera seed oil extraction on human gingival fibroblast cell line Sirinthip Choonate, Suwanna Korsuwannawong, Kongthawat Chairatvit
M Dent J Volume 34 Number 3 September-December 2014
376 Evaluation of the proliferative effect of clinacanthus nutans glycerine and moringa oleifera seed oil extraction on human gingival fibroblast cell line Sirinthip Choonate, Suwanna Korsuwannawong, Kongthawat Chairatvit
M Dent J Volume 34 Number 3 September-December 2014
377Evaluation of the proliferative effect of clinacanthus nutans glycerine and moringa oleifera seed oil extraction on human gingival fibroblast cell line Sirinthip Choonate, Suwanna Korsuwannawong, Kongthawat Chairatvit
M Dent J Volume 34 Number 3 September-December 2014
Table 1 Type, brands and manufacturers of fi ve preservative solution used in this experimentType Brand name/ Cat. No./ Lot No. Manufacturers
Control
DMEM, Dulbecco’s Modified
Eagle Medium Powder
สมุนไพรกลีเซอรีนพญายอ
อภัยภูเบศร
สมุนไพรน้ำมันเมล็ดมะรุม 100%
(100% Pure Moringa Seed Oil)
GIBCOTM Cat. No. 12100-038
Lot No.1154412
อภัยภูเบศร
เลขทะเบียน G668/45
Lot. No.SLG B252
MoringaTM Cat.No. 365
Grand Island, New York, USA.
สำนักงานโรงพยาบาลเจาพระยาอภัยภูเบศร,
จ.ปราจีนบุรี, ประเทศไทย
หจก.สามัญ นีโอ โมริ่งกา,
กรุงเทพฯ, ประเทศไทย
378 Evaluation of the proliferative effect of clinacanthus nutans glycerine and moringa oleifera seed oil extraction on human gingival fibroblast cell line Sirinthip Choonate, Suwanna Korsuwannawong, Kongthawat Chairatvit
M Dent J Volume 34 Number 3 September-December 2014
379Evaluation of the proliferative effect of clinacanthus nutans glycerine and moringa oleifera seed oil extraction on human gingival fibroblast cell line Sirinthip Choonate, Suwanna Korsuwannawong, Kongthawat Chairatvit
M Dent J Volume 34 Number 3 September-December 2014
Table 2 Percent cells viability of Clinacunthus nutans and Moringa oleifera seed oilConcentrations of samples Clinacunthus nutans Moringa oleifera seed oil
Negative control (ThermanoxTM coverslips) 95.11±3.75 109.73± 4.48
0.01% (v/v) 104.08±4.90 108.55± 4.10
0.1% (v/v) 104.48±3.20 110.36± 3.31
0.5% (v/v) 97.55±2.50 107.60± 2.60
Positive control Polyvinylchloride (PVC) 2.93±0.14 2.62± 0.13
Figure 1 Percent cells viability of Clinacanthus nutans Glycerine and Moringa oleifera seed oil
380 Evaluation of the proliferative effect of clinacanthus nutans glycerine and moringa oleifera seed oil extraction on human gingival fibroblast cell line Sirinthip Choonate, Suwanna Korsuwannawong, Kongthawat Chairatvit
M Dent J Volume 34 Number 3 September-December 2014
Table 3 Cell Proliferation in HGF cell line of Clinacunthus nutans Concentrations of samples % Cell proliferation of Clinacunthus nutans
Control 100.00± 4.57
0.01% (v/v) 117.85±10.37
0.1% (v/v) 125.00±14.31
0.5% (v/v) 130.35±11.35
Table 4 Cell Proliferation in HGF cell line of Moringa oleifera seed oilConcentrations of samples % Cell proliferation of Moringa oleifera seed oil
Control 100.00± 3.57
0.01% (v/v) 104.28±10.57
0.1% (v/v) 114.28±15.35
0.5% (v/v) 118.57±11.35
381Evaluation of the proliferative effect of clinacanthus nutans glycerine and moringa oleifera seed oil extraction on human gingival fibroblast cell line Sirinthip Choonate, Suwanna Korsuwannawong, Kongthawat Chairatvit
M Dent J Volume 34 Number 3 September-December 2014
Figure 2 Percentage of cell proliferation compared to the Clinacanthus nutans Glycerine at concentrations of 0.01, 0.1 and 0.5% (v/v) on human gingival fibroblast cell line.
382 Evaluation of the proliferative effect of clinacanthus nutans glycerine and moringa oleifera seed oil extraction on human gingival fibroblast cell line Sirinthip Choonate, Suwanna Korsuwannawong, Kongthawat Chairatvit
M Dent J Volume 34 Number 3 September-December 2014
Figure 3 Percentage of cell proliferation compared to the Moringa oleifera seed oil at
concentrations of 0.01, 0.1 and 0.5% (v/v) on human gingival fibroblast cell line.
383Evaluation of the proliferative effect of clinacanthus nutans glycerine and moringa oleifera seed oil extraction on human gingival fibroblast cell line Sirinthip Choonate, Suwanna Korsuwannawong, Kongthawat Chairatvit
M Dent J Volume 34 Number 3 September-December 2014
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