Imaging technologies: available at UCD now and in the next future EUV microscopy - a user’s perspective Dimitri Scholz EUV, 09.11.2011 Begin ab ovo - Simple approaches direct to the goal - Standard methods of sample preparation Brainstorming before Experiments - One hour thinking saves months of frustrations Light-Electron marriage
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EUV microscopy - a user’s perspective Dimitri Scholz EUV ... · EUV microscopy - a user’s perspective Dimitri Scholz EUV, 09.11.2011 Begin ab ovo - Simple approaches direct to
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Imaging technologies: available at UCD now and in the next future
EUV microscopy - a user’s perspective Dimitri Scholz EUV, 09.11.2011
Begin ab ovo - Simple approaches direct to the goal - Standard methods of sample preparation Brainstorming before Experiments - One hour thinking saves months of frustrations Light-Electron marriage
Abbe/Zeiss 1875
Nikon 80i (2008)
Glorious history of microscopy
Carl Zeiss MicroImaging GmbH, TASC
Light microscopy Electron
microscopy
Make me a confocal picture!
Fluorescent light Transmission light
Bright field
Polarized light
Nomarski contrast (DIC)
Epi-fluorescent Two-Photon Confocal
Structured
Illumination
TIRF SPIM
Decon-
volution
Parallel array
Single pinhole
Spinning disc
Total Internal Reflection Fluorescence
Single Plane Illumination Microscopy
Light microscopy Electron microscopy
Biomed Materials
80 kEV 200-300
kEV
Resolution 0.1 nm
FEG cathode
Please no Eierliegende Wollmilchsau!
TEM
Resolution 1 nm
W or LaB6 cathode
SEM
High spec
80-300 kEV
Resolution ca. 1 nm
FEG cathode
FIB
EDAX X-ray
Fluor Frozen Low vac
Environmental
Table - top
5-30 kEV
Resolution ca. 5-20 nm
W cathode
BSE
E2
BSE
E2
Mean diameter of mouse capillaries Control 2.6 ± 0.07 mm Epo +/+ 3.2 ± 0.06 mm
Scholz and Schaper 2005 Cardiovasc Res 65: 513-23
Transmission light microscopy
1.St occlusion
preconditioned
20 m
B
20 µm
20 µm 20 µm
0
20
40
60
80
100 Diameter, µm
0
500
1000
1500
2000
1d 2d 3d
Wall area, µm2
Growth of collateral arteries continues under zero shear stress