加藤研究室・大岡研究室・菊本研究室 Kato Lab., Ooka Lab., and Kikumoto Lab. Establishing quantitative evaluation method of contact infection risk using qPCR method Background /Purpose 1/2 = + Real-time PCR Thermal cycler Monitoring Fluorescent substance + Background ■ Increase of cities’ population and Processing of building air in recent years ∴Elevation of infection risk→Need to control infection ■ The mechanism of contact infection is complicated (※1) therefore, the evaluation method is not established yet ■ Development of gene analysis technology and PCR(※2)method ・It is classified as three major infection routes among several infection routes. (Air infection, droplet infection, contact infection) ・Frequent occurrence as typified by norovirus ・It is impossible to predict by CFD unlike air infection and droplet infection ・A method developed by Carrie-Maris in 1983. ・It makes analysis faster , accurate and easier. Among them, this study used real-time PCR method is used in this study. This is a method of monitoring the production process of a PCR product using a fluorescent substance and measuring (quantifying and detecting) the target DNA .(Below figure) ・Adenosine TriPhosphate (: ATP) method has been used as a commonly used method until now, but this method is very difficult to measure because it targets cells contained in all organisms. Purpose ■Establishing quantitative evaluation method of contact infection risk using qPCR method ・Establish analysis method ・Establish experimental method ■Creation of highly accurate infection simulator by combination of behavior simulation and simulator (CFD) of other infection route ※1 Contact infection ※2 PCR(Polymerase Chain Reaction) method 資料 : 地理院地図 参考 : http://kansenyobou.net/ ➢ Infection route Characteristic Main causes Microorganisms Contact infection · Frequent propagation path that propagates through fingers, foods and appliances Norovirus Droplet infection Coughing, sneezing, conversation, etc., propagate by droplet particles It falls to the floor within 1 m. It never keeps floating in the air Influenza virus Legionella spp. Airborn infection Coughing, sneezing, etc. will propagate as droplet nuclei. Floating in the air, scattering by airflow M. tuberculosis