EPIGENOMICS CORE Epigenomics Core Biomedical Research Core Facilities MSRB II 2568 1150 W. Medical Center Drive Ann Arbor, MI 48109-0674 OVER The Epigenomics Core provides sample quality control, library preparation and sequencing for the study of cytosine and histone modifications. Products & Services We offer a wide variety of services in epigenomics and bioinformatics resources, including: • Genome-wide DNA Methylation Profiling ❍ Whole-genome bisulfite sequencing: whole-genome base-pair resolution quantification of DNA methylation ❍ ERRBS (Enhanced Reduced Representation Bisulfite Sequencing): Quantitative base-pair measurement of cytosine methylation at ~3M CpGs genome-wide located at promoters, CpG islands and shores, and intergenic regulatory regions ❍ meDIP-seq: Antibody-based method for identification of mC-enriched areas across the genome. ❍ Support with data analysis • Targeted (locus-specific) DNA Methylation ❍ Primer design consultation and training ❍ Bisulfite conversion of DNA and PCR amplification of target sequences ❍ MassARRAY EpiTYPER sequencing ❍ Pyrosequencing ❍ Support with data analysis • Chromatin Modification: ChIP-Seq ❍ Library preparation of user-provided immunoprecipitated DNA ChIP-seq data analysis ❍ Histone Modification by ChIP-Seq (limited initial availability) • Hydroxymethylation ❍ Genome-wide and Target Specific Email the Core to set up a consultation, including a FREE initial consultation to scope your project and ascertain how best to your meet your specific project needs.