ring & Technology - eynzmes designed for industry - Biocatalyc Synthesis - enzymac soluons for chemical problems - Enzyme Engineering & Technology - eynzmes designed for industry - Biocatalyc Synthesis Biocatalyc Synthesis enzymac soluons for chemical problems Enzyme Engineering & Technology enzymes designed for industry science is our success
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11Biocatalytic Synthesis - enzymatic solutions for chemical problems - Enzyme Engineering & Technology - eynzmes designed for industry - Biocatalytic Synthesis - enzymatic solutions for chemical problems - Enzyme Engineering & Technology - eynzmes designed for industry - Biocatalytic Synthesis - enzymatic solutions for chemical problems - Enzyme Engineering & Technology - eynzmes designed for industry - Biocatalytic Synthesis - enzymatic solutions
Biocatalytic Synthesis
enzymatic solutions for chemical problems
Enzyme Engineering & Technology
enzymes designed for industry
science is our success
2Biocatalytic Synthesis - enzymatic solutions for chemical problems - Enzyme Engineering & Technology - eynzmes designed for industry - Biocatalytic Synthesis - enzymatic solutions for chemical problems - Enzyme Engineering & Technology - eynzmes designed for industry - Biocatalytic Synthesis - enzymatic solutions for chemical problems - Enzyme Engineering & Technology - eynzmes designed for industry - Biocatalytic Synthesis - enzymatic solutions
32Biocatalytic Synthesis - enzymatic solutions for chemical problems - Enzyme Engineering & Technology - eynzmes designed for industry - Biocatalytic Synthesis - enzymatic solutions for chemical problems - Enzyme Engineering & Technology - eynzmes designed for industry - Biocatalytic Synthesis - enzymatic solutions for chemical problems - Enzyme Engineering & Technology - eynzmes designed for industry - Biocatalytic Synthesis - enzymatic solutions
Contents
Reductive Biotransformations 4-5
Oxidative Biotransformations 6-7
C-C Bond Formation 8-9
C-C Bond Breaking 9
Enzymatic Isomerisation 10
Hydrolysis / Esterification 11-12
Enzymatic Cascades 12-13
Glycozyme Technology 14-15
API Modification 16
In-silico Search for Novel Biocatalysts 17
About acib 18
Contact 19
4Biocatalytic Synthesis - enzymatic solutions for chemical problems - Enzyme Engineering & Technology - eynzmes designed for industry - Biocatalytic Synthesis - enzymatic solutions for chemical problems - Enzyme Engineering & Technology - eynzmes designed for industry - Biocatalytic Synthesis - enzymatic solutions for chemical problems - Enzyme Engineering & Technology - eynzmes designed for industry - Biocatalytic Synthesis - enzymatic solutions
Reduction of Carboxylic Acids
(CAR = carboxylate reductase, ADH = alcohol dehydrogenase; ChemCatChem 2014, 6, 1089-1095; Biotechnol. J. 2014, 9, 822-843; Chemical Monthly, 2016, 147, 575-578; Adv. Synth. Catal., 2016, in press, DOI: 10.1002/adsc.201600914.)
54Biocatalytic Synthesis - enzymatic solutions for chemical problems - Enzyme Engineering & Technology - eynzmes designed for industry - Biocatalytic Synthesis - enzymatic solutions for chemical problems - Enzyme Engineering & Technology - eynzmes designed for industry - Biocatalytic Synthesis - enzymatic solutions for chemical problems - Enzyme Engineering & Technology - eynzmes designed for industry - Biocatalytic Synthesis - enzymatic solutions
Reductive BiotransformationsReductive Amination of Aldehydes and Ketones
6Biocatalytic Synthesis - enzymatic solutions for chemical problems - Enzyme Engineering & Technology - eynzmes designed for industry - Biocatalytic Synthesis - enzymatic solutions for chemical problems - Enzyme Engineering & Technology - eynzmes designed for industry - Biocatalytic Synthesis - enzymatic solutions for chemical problems - Enzyme Engineering & Technology - eynzmes designed for industry - Biocatalytic Synthesis - enzymatic solutions
76Biocatalytic Synthesis - enzymatic solutions for chemical problems - Enzyme Engineering & Technology - eynzmes designed for industry - Biocatalytic Synthesis - enzymatic solutions for chemical problems - Enzyme Engineering & Technology - eynzmes designed for industry - Biocatalytic Synthesis - enzymatic solutions for chemical problems - Enzyme Engineering & Technology - eynzmes designed for industry - Biocatalytic Synthesis - enzymatic solutions
8Biocatalytic Synthesis - enzymatic solutions for chemical problems - Enzyme Engineering & Technology - eynzmes designed for industry - Biocatalytic Synthesis - enzymatic solutions for chemical problems - Enzyme Engineering & Technology - eynzmes designed for industry - Biocatalytic Synthesis - enzymatic solutions for chemical problems - Enzyme Engineering & Technology - eynzmes designed for industry - Biocatalytic Synthesis - enzymatic solutions
98Biocatalytic Synthesis - enzymatic solutions for chemical problems - Enzyme Engineering & Technology - eynzmes designed for industry - Biocatalytic Synthesis - enzymatic solutions for chemical problems - Enzyme Engineering & Technology - eynzmes designed for industry - Biocatalytic Synthesis - enzymatic solutions for chemical problems - Enzyme Engineering & Technology - eynzmes designed for industry - Biocatalytic Synthesis - enzymatic solutions
10Biocatalytic Synthesis - enzymatic solutions for chemical problems - Enzyme Engineering & Technology - eynzmes designed for industry - Biocatalytic Synthesis - enzymatic solutions for chemical problems - Enzyme Engineering & Technology - eynzmes designed for industry - Biocatalytic Synthesis - enzymatic solutions for chemical problems - Enzyme Engineering & Technology - eynzmes designed for industry - Biocatalytic Synthesis - enzymatic solutions
1110Biocatalytic Synthesis - enzymatic solutions for chemical problems - Enzyme Engineering & Technology - eynzmes designed for industry - Biocatalytic Synthesis - enzymatic solutions for chemical problems - Enzyme Engineering & Technology - eynzmes designed for industry - Biocatalytic Synthesis - enzymatic solutions for chemical problems - Enzyme Engineering & Technology - eynzmes designed for industry - Biocatalytic Synthesis - enzymatic solutions
12Biocatalytic Synthesis - enzymatic solutions for chemical problems - Enzyme Engineering & Technology - eynzmes designed for industry - Biocatalytic Synthesis - enzymatic solutions for chemical problems - Enzyme Engineering & Technology - eynzmes designed for industry - Biocatalytic Synthesis - enzymatic solutions for chemical problems - Enzyme Engineering & Technology - eynzmes designed for industry - Biocatalytic Synthesis - enzymatic solutions
Enantioselective Ester Formation in Aqueous Systems
(AT= acyl transferase; to be published.)
Hydrolysis / Esterification
Enzymatic Phosphorylation
(PPi = phyrophosphate; Eur. J. Org. Chem., 2016, 45–50.)
Chemoenzymatic Preparation of Bio-Based Anti-Oxidants
1312Biocatalytic Synthesis - enzymatic solutions for chemical problems - Enzyme Engineering & Technology - eynzmes designed for industry - Biocatalytic Synthesis - enzymatic solutions for chemical problems - Enzyme Engineering & Technology - eynzmes designed for industry - Biocatalytic Synthesis - enzymatic solutions for chemical problems - Enzyme Engineering & Technology - eynzmes designed for industry - Biocatalytic Synthesis - enzymatic solutions
14Biocatalytic Synthesis - enzymatic solutions for chemical problems - Enzyme Engineering & Technology - eynzmes designed for industry - Biocatalytic Synthesis - enzymatic solutions for chemical problems - Enzyme Engineering & Technology - eynzmes designed for industry - Biocatalytic Synthesis - enzymatic solutions for chemical problems - Enzyme Engineering & Technology - eynzmes designed for industry - Biocatalytic Synthesis - enzymatic solutions
Regioselective Glycosyltransfer
(Biocat. Biotrans. 2010, 28, 10; Pure Appl. Chem. 2013, 85, 1865; PCT Int. Appl. 2015, WO 2015001033.)
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16Biocatalytic Synthesis - enzymatic solutions for chemical problems - Enzyme Engineering & Technology - eynzmes designed for industry - Biocatalytic Synthesis - enzymatic solutions for chemical problems - Enzyme Engineering & Technology - eynzmes designed for industry - Biocatalytic Synthesis - enzymatic solutions for chemical problems - Enzyme Engineering & Technology - eynzmes designed for industry - Biocatalytic Synthesis - enzymatic solutions
Chemo- & Regioselective Oxidation of Soft Nucleophiles
1716Biocatalytic Synthesis - enzymatic solutions for chemical problems - Enzyme Engineering & Technology - eynzmes designed for industry - Biocatalytic Synthesis - enzymatic solutions for chemical problems - Enzyme Engineering & Technology - eynzmes designed for industry - Biocatalytic Synthesis - enzymatic solutions for chemical problems - Enzyme Engineering & Technology - eynzmes designed for industry - Biocatalytic Synthesis - enzymatic solutions
In-silico Search for Novel BiocatalystsTraditional screening for novel enzymes requires time-consuming experiments and expensive activity assays in the wet-lab. To reduce costs, the prediction and identification of enzyme func-tionalities is a major challenge of modern bioinformatics. However, the computational annota-tion of proteins proves to be difficult, erroneous and lacks the possibility to identify completely independent novel biocatalysts because they rely on the correlation of (sequence) similarities with the known functions of the template and are bound to find “more of the same”.
Catalophore Search for Novel Enzymes
acib-researchers developed a patented bioinformatics method1 to mine structural databases using three dimensional search templates which cover the arrangement of chemical functio-nal groups or pre-calculated point-clouds representing the “empty space” of active sites. These search templates are termed “catalophores” (i.e. carrier of the catalytic function). The searches are independent of structural or sequence similarities to currently employed enzymes. Therefo-re, these identified enzymes may feature different physico-chemical properties such as stability, selectivity or substrate tolerance.
A successful test-case led to the identification of two “novel” ene-reductases2, by searching with patterns obtained from classical old yellow enzymes. The identified enzymes showed significant conversions on typical old yellow enzyme substrates and even allowed access to enantiomers that could not be obtained using current enzyme portfolio, although the overall sequence and structural similarity are below 10%.
Catalophore used for the identification of the old-yellow enzyme example; a) schematic mechanism of the reaction mechanism; b) 3D active site constella-tion (“catalphore“) ; c) catalophore mo-tif indicating used atom types; d) geo-metrical representation of the search motif used for the database search.
(1. PCT/EP2013/074556, 2014, ACIB GmbH and University of Graz; 2. Nat. Commun. 2014, 5, 4150.)
18Biocatalytic Synthesis - enzymatic solutions for chemical problems - Enzyme Engineering & Technology - eynzmes designed for industry - Biocatalytic Synthesis - enzymatic solutions for chemical problems - Enzyme Engineering & Technology - eynzmes designed for industry - Biocatalytic Synthesis - enzymatic solutions for chemical problems - Enzyme Engineering & Technology - eynzmes designed for industry - Biocatalytic Synthesis - enzymatic solutions
next step in industrial research
The Austrian Centre of Industrial Biotechnology (acib) is an international research center with 200+ employees based in Austria with with collaborative links to Germany, Slovenia, Italy, Spain and Poland. acib can draw on 25+ ye-ars of experience to apply sophisticated, new methods in industrial production – focused on biocatalysis, enzymes, polymers, (pharmaceutical) protein production & purifica-tion, cell line development, bioinformatics and synthetic biology.
Together with 130+ industrial and scientific partners acib adopts the tools and methods of nature for i) new produc-tion processes & products with improved ecological effici-ency, ii) new production processes with higher economic efficiency, iii) products with higher quality and purity, iv) functional innovative products for everyday use and v) sophisticated computational methods for the health care, pharmaceutical or chemical industry...
acib – science ist our success.
about acib
The competence centre acib is funded in the framework of COMET – Competence Centers for Excellent Technologies – by BMVIT, BMWFW and the provinces of Lower
Austria, Styria, Tyrol and Vienna. The COMET programme is conducted by FFG.
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acib GmbHc/o Department of Chemistry, University of Graz8010 GrazAustria
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24Biocatalytic Synthesis - enzymatic solutions for chemical problems - Enzyme Engineering & Technology - eynzmes designed for industry - Biocatalytic Synthesis - enzymatic solutions for chemical problems - Enzyme Engineering & Technology - eynzmes designed for industry - Biocatalytic Synthesis - enzymatic solutions for chemical problems - Enzyme Engineering & Technology - eynzmes designed for industry - Biocatalytic Synthesis - enzymatic solutions