Enter data as shown

a. Enter data as shown

b.Selectedqrea in blue

Exp. 5, Part B

c. Chart Wizard will generate this graph (I also used a trendline for each set of points ).

Step 1

Reaction rates

Step 2

a. Pick two points on the 0.5mM line

I chose these two points

b. Record the x and y values for each point

Here, X2= 15 min X1= 5 min Y2= 0.4 units Y1= 0.23 units

Y2, X2

Y1, X1

c. Calculate slope of line

= (Y2 – Y1)/ X2 –X1)

=(0.4-0.23)/ 15-5)

=0.17/10min or 0.017units/min

d. Now do the same to calculate the slope for every other line.

Step 3

a. Enter slope of each line on a tableIn Chart Wizard, highlight blue area and select XY scatter and this style to make a graph.

Note that the rate for 2.5 mM substrate is unexpected . Even so, conclusions can be made about the overall effect of substrate concentration on reaction rates.