Electrostatic Potential on HLA-DPB1*1701 and HLA- DPB1*0401: Implications for Putative Mechanism of Chronic Beryllium Disease Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Morgantown, WV James A. Snyder, Ainsley Weston, Sally S. Tinkle and Eugene Demchuk
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Electrostatic Potential on HLA-DPB1*1701 and HLA-DPB1*0401: Implications for Putative Mechanism of Chronic Beryllium Disease Health Effects Laboratory.
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Electrostatic Potential onHLA-DPB1*1701 and HLA-
DPB1*0401:Implications for Putative
Mechanism of Chronic Beryllium Disease
Health Effects Laboratory Division,
National Institute for Occupational Safety and Health,
Morgantown, WV
James A. Snyder, Ainsley Weston, Sally S. Tinkle and Eugene Demchuk
Objective
to understand the physical-chemical reasons for beryllium toxicity
in Chronic Beryllium Disease (CBD)
Periodic TableWhat makes Be toxic?
The Test Hypothesis
Be2+ binds to HLA-DP and changes the specificity of molecular recognition
Goal: To identify and test the Be2+ binding mode
Experimental Evidence
• Amicosante, M., Sanarico, N. Berretta, F., Arroyo, J., Lombardi, G., Lechler, R., Colizzi, V. and Saltini, C. Beryllium Binding to HLA-DP Molecule Carrying the Marker of Susceptibility to Berylliosis Glutamate 69. Human Immunology, 62, 686-693 (2001)
• Fontenot, A.P., Torres, M., Marshall, W.H., Newman, L.S. and Kotzin, B.L. Beryllium Presentation to CD4+ T Cells Underlies Disease-Susceptibility HLA-DP Alleles in Chronic Beryllium Disease. Proc. Natl. Acad. Sci. USA, 97, 12717-12722 (2000)
MHC class II
cellmembrane
Crystal Structure of Human Class II MHC Proteins
in the Protein Data Bank (PDB)HLA-DR1
• 1AQD HLA-DR1 (DRA, DRB1 0101) Human MHC Class II complexed with endogenous peptide• 1DLH HLA-DR1 (DRA, DRB1 0101) Human Class II histocompatibility protein (extracellular domain)• 1FYT HLA-DR1, DRB1 0101• 1HQR DRA*0101 + DRB5*0101• 1KG0 the Epstein-Barr virus Gp42 Protein bound to the MHC Class II HLA-DR1• 1SEB complex of the Human MHC Class II Glycoprotein HLA-DR1 and the bacterial superantigen SEB
HLA-DR2• 1BX2 HLA-DR2 (DRA*0101, DRB1*1501) complexed with a peptide from Human myelin basic protein
1FV1 immunodominant peptide from myelin basic protein in different registers by two HLA-DR2 allelesHLA-DR3
• 1A6A clip bound to HLA-DR3HLA-DR4
• 1D5M HLA-DR4 complexed with peptide and SEB• 1D5X HLA-DR4 complexed with dipeptide mimetic and SEB• 1D5Z HLA-DR4 complexed with peptidomimetic and SEB• 1D6E HLA-DR4 complex with peptidomimetic and SEB• 1J8H HLA-DR1, DRA + HLA-DR4, DRB1*0401• 2SEB HLA-DR4 complexed with a peptide from Human collagen II
HLA-DQ8• 1JK8 Human insulin peptide-HLA-DQ8 complex
Sequence Alignment of HLA-DPB1*1701 with HLA-DPB1*0401
-chain
Class II MHC antigen-binding Pocket
-chain
Poisson-Boltzmann Equation
Finite Difference PB Methods
S V V
ε dS εk dV ρdV
V V V
ε dS εk dV ρdV
using divergence theorem:
6
2 3 2
1i j i j S i
j
q h ε h ε I
HLA-DPA1*01031/B1*0401 (model)
Electrostatic Potential Surface
Map
HLA-DPA1*01031/B1*1701 (model)
Electrostatic Potential Surface
Map
*1701 MEP Movie
*0401 MEP Movie
1701 MEP Movie
Conclusion
Our results are consistent with the hypothesis that glutamic acid at position 69 of the -chain of HLA-DP may be a determinant of susceptibility to Chronic Beryllium Disease
James A. Snyder
Eugene Demchuk
Ainsley Weston genetics
Sally S. Tinkle immunology
People
Health Effects Laboratory Division,
National Institute for Occupational Safety and Health,