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Electrochemical Detection in HPLC 1
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Electrochemical Detection in HPLC

Feb 25, 2016

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Electrochemical Detection in HPLC. ALEXYS ™ - HPLC/ECD. Applications Neuroscience Pharma Clinical analysis Food & Beverages Environmental. Why ECD?. extremely sensitive highly selective . Principle of HPLC/ECD. electrochemical reaction detector potential (E) is driving force. - PowerPoint PPT Presentation
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Page 1: Electrochemical Detection in HPLC

Electrochemical Detection in HPLC

1

Page 2: Electrochemical Detection in HPLC

ALEXYS™ - HPLC/ECD

ApplicationsNeurosciencePharmaClinical analysisFood & BeveragesEnvironmental

2

Page 3: Electrochemical Detection in HPLC

3

Why ECD?

extremely sensitivehighly selective

Page 4: Electrochemical Detection in HPLC

4

Principle of HPLC/ECDelectrochemical reaction detectorpotential (E) is driving force

Page 5: Electrochemical Detection in HPLC

5

Electrochemical reaction

ΔE

diffusion electron transfer reaction

Page 6: Electrochemical Detection in HPLC

Facilitates the EC reaction

6

Role of electrode potential E

0

20

40

60

80

100

0 400 800 1200 1600

E (mV)

Sig

nal (

%)

no reaction

oxidation occurs

A B

Page 7: Electrochemical Detection in HPLC

Working range potential E

E working range determined by mobile phase

7

Page 8: Electrochemical Detection in HPLC

8

Peak height vs. concentration

UV Beer’s law E = d C

ECD Cottrell equation ilim= 1.47 n F (Fm )1/3 (DA/b)2/3 C

A= WE area Ab = spacer thicknessC = concentrationilim= diffusion limited current measured (peak height)

peak height (E) vs concentration (C)

peak height (Ilim) vs concentration (C)

Page 9: Electrochemical Detection in HPLC

9

Peak height vs. concentration

UV Beer’s law E = d C

ECD Cottrell equation ilim= f (A/b)2/3 C

A= WE area Ab = spacer thicknessC = concentrationilim= peak height

as simple as abc…

Page 10: Electrochemical Detection in HPLC

Electroactive Groups

10

Page 11: Electrochemical Detection in HPLC

Application areas…

11

www.myAntec.com

Page 12: Electrochemical Detection in HPLC

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Summary

Electrochemical Detection for HPLC

Potential drivenSelectiveSensitive

Thank you!