The world leader in serving science M. De Pra, G. Greco, N. Bartsch, E. George, M. M. Martin, F. Steiner Thermo Fisher Scientific, Germering (Germany), Bremen (Germany), San Jose (USA) Effects of Metal Contamination Caused by Iron- Free HPLC Systems on Peak Shape and Retention of Drugs with Chelating Properties For research use only. Not for use in diagnostic procedures.
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Effects of Metal Contamination Caused by Iron-Free HPLC ...€¦ · • Without salt - Contaminated column is much less retentive With salt - Column behavior becomes more similar
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The world leader in serving science
M. De Pra, G. Greco, N. Bartsch, E. George, M. M. Martin, F. SteinerThermo Fisher Scientific, Germering (Germany), Bremen (Germany), San Jose (USA)
Effects of Metal Contamination Caused by Iron-Free HPLC Systems on Peak Shape and Retention of Drugs with Chelating Properties
For research use only. Not for use in diagnostic procedures.
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Schematic of fluidic components in a HPLC
Biocompatible HPLC Systems
• Biocompatible (U)HPLCs are used in the analysis of biomolecules
• Biomolecules are often analyzed with high salt concentrations in the mobile phase
• Conventional stainless steel HPLCs form rust
• In biocompatible UHPLC titanium is used instead of stainless steel
Is titanium always inert?
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Titanium weakness
Stress Corrosion Cracking ofTi in pure MeOH
• Relevant problem in material science
• Not a problem for UHPLC systems
Any effect on chromatography?
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Ti Leaching from a Biocompatible System
• ICP-MS analysis of effluents collected w/o column: 2 ppb Ti
• All Ti fluidic components contribute to Ti leaching
• The solvent frits are most critical (very large surface)
How do Ti ions affect chromatography?
H2O MeOHACN
A B
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7 fluoroquinolones thiamphenicol
Prone to tailing No tailing
Investigating the Mechanism: The Experiments
• ISOCRATIC (H2O, MeOH, ACN, formic acid) pH 2.9
• Non-corrosive conditions (Any part of fluidic always in contact with water)