0 EFEK PEMBERIAN KOMBINASI KURKUMIN DAN PUFA OMEGA-3 TERHADAP ADIPONEKTIN PADA SUBYEK TOLERANSI GLUKOSA TERGANGGU EFFECT OF ADMINISTRATION OF COMBINATION OF CURCUMIN AND OMEGA-3 PUFA ON ADIPONECTIN IN IMPAIRED GLUKOSE TOLERANCE SUBJECT Ni Made Dwi Asti Lestari, Suryani As’ad, Agussalim Bukhari Bagian Gizi Fakultas Kedokteran Universitas Hasanuddin, Makassar Alamat Korespondensi: Ni Made Dwi Asti Lestari Fakultas Kedokteran Universitas Hasanuddin Makassar, 90245 HP: 08124602941 Email: [email protected]
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EFEK PEMBERIAN KOMBINASI KURKUMIN DAN PUFA OMEGA-3 TERHADAP ADIPONEKTIN
PADA SUBYEK TOLERANSI GLUKOSA TERGANGGU
EFFECT OF ADMINISTRATION OF COMBINATION OF CURCUMIN AND OMEGA-3 PUFA
ON ADIPONECTIN IN IMPAIRED GLUKOSE TOLERANCE SUBJECT
Ni Made Dwi Asti Lestari, Suryani As’ad, Agussalim Bukhari
Bagian Gizi Fakultas Kedokteran Universitas Hasanuddin, Makassar
Alamat Korespondensi: Ni Made Dwi Asti Lestari Fakultas Kedokteran Universitas Hasanuddin Makassar, 90245 HP: 08124602941 Email: [email protected]
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ABSTRAK
Penanganan terhadap toleransi glukosa terganggu.dapat mencegah terjadinya diabetes militus tipe 2. Tujuan penelitian ini adalah untuk mengetahui efek pemberian kombinasi kurkumin dan PUFA Omega-3 terhadap adiponektin pada subyek toleransi glukosa terganggu. Metode penelitian ini adalah merupakan suatu penelitian uji klinis acak terkontrol tersamar ganda. Subyek Penelitian:Laki-laki dan perempuan, umur 35-55 tahun, IMT (>25 kg/m2), TTGO 140-199 mg/dl. Dengan pemberian kapsul kombinasi kurkumin dan PUFA omega 3 atau plasebo. Kemudian dilakukan pemeriksaan adiponektin sebelum dan sesudah pemberian kapsul. Hasil yang diperoleh diuji secara statistik dengan tingkat kemaknaan < 0.05. Hasil penelitian menunjukkan bahwa subyek yang dibandingkan tidak berbeda dalam hal umur, IMT, TTGO. Asupan energi, karbohidrat, protein, lemak dan PUFA tidak berbeda antara kelompok perlakuan dan kontrol. Terjadi peningkatan kadar adiponektin yang lebih tinggi pada kelompok intervensi dibandingkan kelompok plasebo tetapi tidak bermakna secara statiatik (p=0.42).Terjadi penurunan berat badan di akhir penelitian. Kurkumin dan PUFA omega 3 dapat memperbaiki toleransi glukosa terganggu dengan meningkatkan adiponektin. Pada penelitian ini tidak terjadi peningkatan secara bermakna untuk Adiponektin yang kemungkinan disebabkan oleh adanya kontrol terhadap asupan pada kedua kelompok. Kesimpulanpenelitian ini adalah hasil penelitian ini mendukung pemberian kurkumin dan PUFA omega 3 untuk perbaikan toleransi glukosa terganggu. Kata kunci: PUFA omega 3, kurkumin, Toleransi glukosa terganggu, adiponektin ABSTRACT Impaired glucose tolerance treatment was prevent type 2 diabetes mellitus.. The research aimed to investigate the effect of curcumin and omega-3 PUFA on adiponectin in impaired glucose tolerance subjects. The Research methods was multiple blinded controlled randomized clinical trial test with crossover design. The Research subjects were Males and females, 35-55 years old, the body mass index (BMI) was > 25 kg/m2, the oral glucose tolerance test (OGTT) was 140-199 mg/dl. The administration of capsule of the combination of curcumin and omega-3 PUFA or placebo and the Adiponectin examination were carried out before and after the intervention administration. The results obtained was examined by statistics test which was suitable with the significance level p< 0.05.The research result indicates that the compared to subjects are not different in terms of age, BMI, OGTT. the compared to subjects are not different in terms of energy, charbohidrat, protein, fat and PUFA.There is the higher increase of the adiponectin content on in the intervention group compared with the placebo group but it is statiatlicaly insignificant (p = 0.42). Body weight decreased at the end of research. The curcumin and omega-3 PUFA can improve the impaired glucose tolerance by improving the adiponectin content. In the res there research. There is no significance improvement for the Adiponectin which possibly caused by the control on intake of both groups. This study Conclusion suggests that the research results support the administration of the curcumin and omega 3 PUFA for improvement of the impaired glucose tolerance. Keywords: Omega-3 PUFA, curcumin, impaired glucose tolerance, adiponectin
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PENDAHULUAN
Terdapat bukti yang menunjukkan diabetes melitus tipe 2 dapat dicegah dengan
perubahan gaya hidup, atau menurunkan glukosa darah dengan obat-obatan. Sejauh ini, program
pencegahan ini sering targetnya adalah subyek dengan toleransi glukosa terganggu. Toleransi
glukosa terganggu (TGT) berhubungan erat dengan proses obesitas yang menyebabkan
selama 1 minggu meningkatkan kadar adiponektin yang tidak bermakna secara statistik. Perlu
dilakukan penelitian dengan pemberian dosis dan waktu yang lebih bervariasi sehingga
didapatkan dosis dan waktu yang optimal untuk mendapatkan efek sinergis dari kurkumin dan
PUFA omega 3.
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Tabel 1. Karakteristik Data
Variabel Perlakuan
Kontrol
p
Jenis kelamin(%) Laki-laki perempuan
3(15)
7(35)
2(10)
8(40)
Umur (tahun) 44,10+4,84
43,20+5,59
0,37*
Tinggi badan(cm)
158,20+6,75
154,0+8,97
0,31*
Berat badan(kg) 72, 62+8,30
71,38+9,58
0,44*
IMT(kg/m2) 30, 43+3,06
28,47+2,51
0,67*
OGTT(mg/dl) 158,80+15,45
154,80+10.99
0,57*
Adiponektin(ng/ml) 21,70 (1,21-193,58)
24,09 (4,03-117,39)
0.85**
*Uji t tidak berpasangan, **uji Mann Whitney, subyek penelitian 20 orang, dan p signifikan < 0.05
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Tabel 2. Uji Asupan Makanan antara kelompok A dan Kelompok B
Variabel Hari Perlakuan Mean +SD
Kontrol Mean +SD
p
Energi(kkal) 1
7
1586.20+151.28
1615.70+203.30
1633.40+191.68
1573.90+126.99
0.21*
0.07*
Karbohidrat (g)
1
7
242.23+34.31
234.45+33.36
243.27+37.27
225.15+22.97
0.75*
0.32**
Protein(g) 1
7
61.72+17.65
70.73+19.16
66.48+17.03
68.17+16.73
0.42*
0.37*
Lemak(g) 1
7
40.92+12.04
43.37+12.67
46.80+12.81
47.94+11.18
0.59*
0.28*
PUFA(g) 1
7
12.97+7.15
11.96+6.81
13.86+8.92
13.46+6.29
1.48*
0.05*
*Uji t tidak berpasangan,**Uji Mann Whitney, p signifikan <0.05
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Tabel 3. Perubahan berat badan dan IMT subyek penelitian
Variabel Perlakuan Kontrol
p
BB pre(kg) BBpost(kg) BBselisih(kg)
71,85+8,85
71,48+8,95
-0,35(-1,7 – 0,5)
71,21+ 9,06
71,21+9,03
-0,29-0,8 – 1,7)
0.95*
0,10*
0,12**
IMT pre(kg/m2) IMT post(kg/m2) IMT selisih(kg/m2)
28,60(25,5-35,9)
29,3+2,80
-0,15(-0,8 – 0,9)
28,70( 24,90-35,60)
29,11+9,99
-0,1(-0,7 – 0,7)
0,73**
0,72*
0,53**
*uji t tidak berpasangan,**uji Mann Whitney, dan p signifikan <0.05
Tabel 4. Perbandingan kadar Adiponektin antara kelompok perlakuan dan kelompok
kontrol
Variabel Perlakuan
n=20 Kontrol
n=20 P
Adiponektin pre(ng/ml)
21,70 (1,21-193,58)
24,09 (4,03-117,39)
0.85
Adiponektin post(ng/ml)
38,66 (6,58-283,01)
35,61 (5,85-117,39
0.54
Adiponektin selisih(ng/ml)
3,95 (-3,7-167,65)
3,26 (-9,02-49,30)
0.42
Uji Mann Whitney, dengan p signifikan < 0.05
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Gambar 1. Perbandingan peningkatan kadar adiponektin antara kelompok perlakuan dan