Ecological Assessment of Lake Istokpoga · 2019. 5. 23. · Ecological Assessment of Lake Istokpoga – May 2019 Page 5 of 96 mean chlorophyll a value of less than 20 μg/L. Annual

Ecological Assessment of Lake Istokpoga – May 2019

Ecological Assessment of

Lake Istokpoga

Highlands County

Sampled July 2016 to June 2017

Division of Environmental Assessment and Restoration

Florida Department of Environmental Protection

May 2019

DEP Laboratory, Biology Program

2600 Blair Stone Road, MS 6515

Tallahassee, FL 32399-2400

www.floridadep.gov


Page 2 of 96

Contents

Contents ..........................................................................................................................................2

Executive Summary .........................................................................................................................3

Overview ..........................................................................................................................................4

Methods............................................................................................................................................7

Results ..............................................................................................................................................7

Discussion ........................................................................................................................................9

Appendix A. Phytoplankton Wet Taxa ..........................................................................................35

Appendix B. Phytoplankton DiatomTaxa ......................................................................................84

List of Tables

Table 1. Measured field parameters. ............................................................................................. 11

Table 2. Laboratory analytical results. .......................................................................................... 15

Table 3. Dominance of algal species. ........................................................................................... 25

Table 4. Phytoplankton taxonomic variables. ............................................................................... 26

Table 5. Dominant wet and diatom taxa. ...................................................................................... 31

List of Figures

Figure 1 Map of Lake Istokpoga in Highlands County, Florida ..................................................... 6

Figure 2. Graph of water temperature. .......................................................................................... 13

Figure 3. Graph of pH. .................................................................................................................. 13

Figure 4. Graph of dissolved oxygen. ........................................................................................... 14

Figure 5. Graph of specific conductance. ..................................................................................... 14

Figure 6. Graph of color (true). ..................................................................................................... 19

Figure 7. Graph of total organic carbon. ....................................................................................... 19

Figure 8. Graph of total suspended solids. .................................................................................... 20

Figure 9. Graph of turbidity. ......................................................................................................... 20

Figure 10. Graph of ortho-phosphate. ........................................................................................... 21

Figure 11. Graph of total phosphorus. .......................................................................................... 21

Figure 12. Graph of total Kjeldahl nitrogen.................................................................................. 22

Figure 13. Graph of ammonia. ...................................................................................................... 22

Figure 14. Graph of nitrate-nitrite. ................................................................................................ 23


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Figure 15. Graph of chlorophyll a, corrected. ............................................................................... 23

Figure 16. Phytoplankton density. ................................................................................................ 24

Figure 17. Comparison of phytoplankton density to chlorophyll a, corrected. ............................ 24

Figure 18. Box and whisker plot of the percent composition by algal group. .............................. 30

Figure 19. Average monthly minimum and maximum precipitation for Highlands County........ 34

Executive Summary

Highlands County Board of County Commissioners (HCBCC), through the Lake Istokpoga

Management Committee (LIMC), requested sampling and analytical assistance from the Florida

Department of Environmental Protection (DEP) for Lake Istokpoga due to reported public

concerns over the health of the waterbody and the potential of harmful algal bloom formation.

Working in collaboration, staff from the DEP’s South Regional Operations Center (SROC) and

Highlands County Parks and Natural Resources (HCPNR) conducted monthly sampling events at

six locations on the lake over the course of one year, July 2016 to June 2017. Field parameters,

water samples, and algal samples were collected at each site and sent for analysis to the DEP

Laboratory in Tallahassee, (DOH Accreditation E31780).

Measured field parameters exhibited similar and/or expected patterns across all 6 locations and

dates. Total suspended solids, turbidity, total Kjeldahl nitrogen, and chlorophyll a tended to

increase over the study period. Lake color decreased over the study however, measurements

were greater than 43 PCU for all sampling dates. For lakes with color greater than 40 PCU, the

annual geometric mean for total phosphorus is to be between the 0.05 and 0.16 mg/L, for total

nitrogen is to be between 1.27 mg/L and 2.23 mg/L, and for chlorophyll a is to be less than 20

µg/L, Chapter 62-302.531(2)(a)(2)(b)1., Florida Administrative Code (F.A.C.). The majority of

single sample nutrient values measured during this study fell within this range however, 67 of the

72 single sample chlorophyll a were greater than 20 µg/L.

Samples were collected to examine whether a bloom condition existed at the time of collection.

Dominant or co-dominant species were present in only 12 of the 72 samples. For those 12

samples, the taxa identified as dominant or co-dominant were Cylindrospermopsis rackiborskii,

Planktolyngbya contorta, Planktolyngbya limnetica, and Microcystis aeruginosa. These are

cyanobacteria species (blue-green algae) that have the potential to produce toxins however,

cyanotoxins were not detected in the 16 samples analyzed. Although potential toxin producing

species may have been present, laboratory results indicate that these species were not producing

toxins at the time of collection.

Additional samples were collected for phytoplankton species identification and enumeration.

With few exceptions, blue-green algae comprised the majority of the phytoplankton samples,

followed by green algae. Algal density varied between the collection dates but generally,

increased over the time, similar to the pattern of increasing chlorophyll a. There was a positive


Page 4 of 96

relationship between increasing phytoplankton density and chlorophyll a. Although algal density

increased over time, the phytoplankton community composition remained fairly consistent

within and among sites, indicating little shift in relative proportions of major groups of algae

over the study period.

The analytical results suggest similar water quality and biological communities among the 6

different locations within the lake, with possibly the exception of the Northeast site. The pattern

of change in both measured field parameters and water quality data reflect the pattern of change

in average monthly rainfall received in Highlands County. This suggests that Lake Istokpoga’s

water quality and algal community is susceptible to, influenced by, and changes in response to

weather-driven patterns.

Overview

DEP Surface Water Quality Standards, Chapter 62-302, Florida Administrative Code (F.A.C.),

are designed to protect the designated uses of waters and exceedances of these standards are

associated with interference with the designated use. DEP uses methods in Chapter 62-303,

F.A.C. to evaluate the condition of a waterbody in relation to the water quality criteria, including

whether adverse impacts to biological communities have occurred. Human stressors that may

negatively impact the condition of a waterbody include increased inputs of nutrients, sediments

and/or pesticides from watershed runoff, undesirable removal of native shoreline and/or upland

buffer vegetation, and introduction of nuisance (generally exotic) plants and animals.

For waterbodies with Class I, II, III and III Limited designations, surface water quality criteria

prohibit the alteration of nutrient concentration “so as to cause an imbalance in natural

populations of aquatic flora and fauna” (62-302.530(48)(b). The criteria also prohibits the

presence of any “substances in concentrations which results in the dominance of nuisance

species” 62-302.530(47) F.A.C.. A variety of field assessment methods and analytical parameters

can be used in conjunction with one another to detect potential stressors and imbalances in the

biological communities. For example, measuring the nutrient concentration in a waterbody may

indicate the potential for excessive plant or algal growth. Measuring the concentration of the

photosynthetic pigment chlorophyll a in a water sample, provides an estimate of algal biomass.

Higher than expected chlorophyll a for a particular waterbody may indicate the presence of algal

bloom conditions which may not only result in unwanted shading of aquatic plants but pose a

human health risk if certain potential toxin producing species are present.

Measuring the concentration of the photosynthetic pigment, chlorophyll a, in a water sample

provides an estimate of algal biomass. For lakes, 62-302.531(2)(a)(2)(b)1., F.A.C. of the

Numeric Interpretations of Narrative Nutrient Criteria describes the applicable interpretation of

the narrative nutrient criteria in paragraph 62-302.530(48)(b), F.A.C. for chlorophyll a. In clear,

low alkalinity lakes (color less than 40 PCU and alkalinity less than 20 mg/L CaCO3), a healthy

system is expected to have a geometric mean chlorophyll a value of less than 6 μg/L. In clear,

high alkalinity lakes (color less than 40 PCU and alkalinity greater than 20 µg/L CaCO3) or in

colored lakes (color greater than 40 PCU), healthy systems are expected to have a geometric


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mean chlorophyll a value of less than 20 μg/L. Annual geometric mean chlorophyll a greater

than these values may result in the waterbody being identified as impaired or not meeting water

quality standards. Higher chlorophyll a indicates greater algal biomass and possibly the presence

of bloom conditions.

Lake Istokpoga is located approximately 7 miles northeast of Lake Placid, FL in Highlands

County (Figure 1). Fed by Arbuckle Creek and Josephine Creek in the north, the lake discharges

into the C41 canal to the south. Lake Istokpoga is part of the Kissimmee watershed and

therefore, lies within the Peninsula Nutrient Watershed Region, 62-302.200(13)(e) F.A.C. The

predominate land use surrounding the lake includes pastures, citrus groves, and residential

dwelling. This lake has a Class III designated use defined as fish consumption; recreation,

propagation and maintenance of a healthy, well-balanced population of fish and wildlife, 62-

302.400 F.A.C.

Using the methods of evaluation in Chapter 62-303, F.A.C., Lake Istokpoga is currently verified

impaired for chlorophyll a, total phosphorous, and total nitrogen and was included on the

verified list of impaired waters adopted June 27, 2017, https://floridadep.gov/dear/watershed-

assessment-section/content/lists-impaired-waters-group-4-basins. Currently, a Total Maximum

Daily Loads (TMDLs) is under development for the lake and is anticipated to be completed by

2022. Additional information about this waterbody is available on DEP ArcGIS Online,

http://fdep.maps.arcgis.com/home/index.html.

https://floridadep.gov/dear/watershed-assessment-section/content/lists-impaired-waters-group-4-basinshttps://floridadep.gov/dear/watershed-assessment-section/content/lists-impaired-waters-group-4-basinshttp://fdep.maps.arcgis.com/home/index.html


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Figure 1 Map of Lake Istokpoga in Highlands County, Florida


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Methods

Working in collaboration, staff from the DEP’s South Regional Operations Center (SROC) and

Highlands County Parks and Natural Resources (HCPNR) conducted monthly sampling events at

six locations on the lake over the course of one year, July 2016 to June 2017. Water quality

readings for temperature, specific conductivity, dissolved oxygen, and pH were conducted at

each site using YSI multi-parameter meters. Direct surface grab samples were collected for

ammonia, chlorophyll a, true color, turbidity, total suspended solids, total organic carbon, nitrate

and nitrite, ortho-phosphate, total phosphorous, algal toxins, and algal species identification.

Water samples were preserved and shipped for analysis to the DEP Laboratory in Tallahassee,

(DOH Accreditation E31780). Due to staff turnover, the October 2016, January 2017, and April

2017 quantitative water samples for algal identification were sent to and analyzed by Greenwater

Labs, Inc..

All field and laboratory methods followed DEP Standard Operating Procedures (see

https://floridadep.gov/dear/quality-assurance/content/dep-sops and

https://floridadep.gov/dear/florida-dep-laboratory/content/dep-laboratory-quality-assurance-

manual-and-sops for details) and met DEP quality assurance/quality control standards (see

https://floridadep.gov/dear/quality-assurance).

All references to 62-302.530, F.A.C. are to the surface water quality criteria table with an

effective date of 02/17/2016 and a notice identification number of 17115667.

Results

Measured field parameters for all 12 sampling dates at the 6 lake locations are presented in Table

1. These parameters exhibited similar patterns across all 6 locations. As expected, water

temperature was highest in the summer months and lowest in the winter months (Figure 2). In

general, measured pH was lowest and less variable across most sites September through

November 2016 (Figure 3). The greatest pH values were measured at most sites March through

June 2017. Dissolved oxygen was recorded as either mg/L or percent saturation. Percent

saturation was calculated to facilitate comparisons across sampling dates. Percent saturation was

higher in the winter and spring months and lower during the summer and fall months (Figure 4).

Specific conductance was only recorded for 6 of the 12 sampling dates. Specific conductance

was slightly lower on the two sampling dates in 2016 than the 4 sampling dates in 2017 (Figure

5).

Analytical chemistry results are presented in Table 2. Graphs for each parameter are included to

help visualize the relationships and patterns in the data. The Northeast site results tended to be

different from other sites for certain sampling dates and parameters. Of note, is that the analytical

results for samples collected at the Northeast site July through September or October 2016 and

especially in June 2017, were greater for ammonia, color, total organic carbon, ortho-phosphate,

and total phosphorus and lower for total suspended solids and turbidity than all the other values

measured at that site and at the other 5 sites during the duration of the study. Also, of note, only

https://floridadep.gov/dear/quality-assurance/content/dep-sopshttp://www.dep.state.fl.us/labs/library/lab_sops.htmhttps://floridadep.gov/dear/florida-dep-laboratory/content/dep-laboratory-quality-assurance-manual-and-sopshttps://floridadep.gov/dear/florida-dep-laboratory/content/dep-laboratory-quality-assurance-manual-and-sopshttps://floridadep.gov/dear/quality-assurance


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1 of 72 samples for nitrate-nitrite had a detection greater than the laboratory practical

quantitation limit (PQL). The result for this 1 sample, collected on November 29, 2016 at the

Northwest site, was 10 times greater than the PQL. All laboratory analytical quality control

associated with this sample was re-reviewed and confirmed passing and therefore, it is either an

anomalous result compared to others in the study or there was possible contamination either in

the field or prior to analysis.

Lake color measurements were greater than 43 PCU for all sampling dates (Figure 6). Color and

total organic carbon showed similar patterns (Figures 6 and 7, respectively). Although consistent

in concentration relative to one another, values for color and total organic carbon were more

variable between the 6 locations from July through October 2016. After, values converged and

remained consistent between sites November 2016 through May 2017 and until the exception of

the June 29, 2017 Northeast site sample. Total suspended solids and turbidity values were similar

across sites from July through December 2016 then, steadily increased until May 2017. Both

total suspended solids and turbidity decreased in the June 2017 samples (Figures 8 and 9,

respectively).

Ortho-phosphate and total phosphorus concentrations at the Northeast site were greater in the

first few months and on the final month of the study than for all other samples collected (Figures

10 and 11, respectively). Ortho-phosphate was detected in only 5 samples at the Northeast site

and was either not detected (59 samples) or detected between the laboratory method detection

limit (MDL) and PQL (8 samples) for all other samples collected during the study. Total

phosphorus was detected at all sites and those values increased over time. With the exceptions of

2 samples collected at Northeast site on August 2016 and June 2017, total phosphorus values

ranged between 0.03 and 0.13 mg/L. Total Kjeldahl nitrogen values were lower in 2016 than

2017 and, in 2017 increased over time (Figure 12). Ammonia and nitrate-nitrite values were low

and consistent over the entire study period, with only 2 sample exceptions. Ammonia was

detected below 0.011 mg/L except in the Northeast June 2017 sample and nitrate-nitrite values

were all less than 0.009 mg/L except in the Northwest November 2016 sample (Figure 13 and

14, respectively). Chlorophyll a is an indicator of algal biomass and is considered a potential

response variable to nutrients. Chlorophyll a concentration, in a similar pattern to the total

suspended solids and turbidity data, slowly increased over time (Figure 15). All but 5 of 72

single sample values were greater than 20 µg/L.

DEP taxonomists examined microscopically aliquots of water samples from each location and

sampling date to determine if a dominant taxon or co-dominant taxa of algae were present at the

time of collection. Dominance of one or few species may indicate the presence of an algal bloom

condition in the waterbody. There was a mixed assemblage and no clear dominance of algal

species in 60 of the 72 samples examined (Table 3). Dominance of one or two species in the

samples was observed on only 4 collection dates, July 2016, October 2016, March 2017, and

May 2017. A dominant species was never observed in the samples from the Northeast site. In

July 2016 and October 2016, dominant and co-dominant species occurred in samples at each of

the other 5 sites. For the 12 samples, the taxa identified as dominant or co-dominant were

Cylindrospermopsis rackiborskii, Planktolyngbya contorta, Planktolyngbya limnetica, and


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Microcystis aeruginosa. These are cyanobacteria species (blue-green algae) that have the

potential to produce toxins.

Toxin analysis was performed if algal species that have the potential to produce toxins were

observed during the microscopic examination of water samples collected to identify if a

dominant algal species was present. Toxins were analyzed by HPLC/MS/MS for samples

collected at all 6 sites for July and August 2016, the Southeast site for October 2016, and the

South, Southeast, and West sites for June 2017. Microcystins and cylindrospermopsin were not

detected in any of the analyzed samples. Anatoxin-a was analyzed in the June 2017 sample only

and was not detected.

A summary of the water samples collected for quantitative phytoplankton taxonomic analysis is

provided in Table 4. Phytoplankton wet taxa and diatoms are identified separately. In the wet

taxa analysis, soft algae are identified to the lowest practical level, and diatoms are identified

only to order level (diatom). In the diatom analysis, diatoms are identified to the lowest practical

level. Taxa lists are provided for the wet algae and diatoms in appendices A and B, respectively.

Algal density is calculated as number of natural units/mL for phytoplankton samples. Algal

density varied between the collection dates but generally, increased over the time, similar to the

pattern of increasing chlorophyll a (Figure 17). The was a positive relationship between

increasing phytoplankton density and chlorophyll a, corrected analytical results.

Phytoplankton community composition is calculated to examine potential shifts in relative

proportions of major groups of algae. With few exceptions, blue-green algae (cyanobacteria)

comprised the majority of the phytoplankton samples, followed by green algae. Two exceptions

were the Northeast July 2016 sample, which contained 44% diatoms and only 16% blue-greens,

and the September 2016 sample, which contained 43% green and 40% blue-green algae. With

the Northeast July 2016 data point removed, the percent of blue-green algae ranged from 41% to

80% and averaged 63% of the sample community. Green algae comprised the next largest

percent of the samples, ranging from 8% to 43% and averaging 22% of the sample community.

The Northeast July 2016 sample also contained 28% Pyrrophytes, which then decreased to 7% in

the sample collected the following month. In all following samples at this site and in all samples

from the other 5 sites, Pyrrophytes comprised less than 0.7%. In the Northeast July 2016 sample,

the percent of green algae across all sites was approximately half the percent green algae found

in all other samples in the study. The dominant taxa identified in the quantitative samples are

presented in Table 5.

Discussion

Measured field parameters exhibited similar and/or expected patterns across all 6 locations and

dates although specific conductance was greater in 2017 than 2016. The analytical results

suggest similar water quality and biological communities among the 6 different locations within

the lake, with possibly the exception of the Northeast site. Measurements of lake color were

greater than 43 PCU for all sampling dates. The majority of single sample nutrient values

measured during this study fell within the range of annual geometric means identified for lakes


Page 10 of 96

with color greater than 40 PCU however, 67 of the 72 single sample chlorophyll a values were

greater than the 20 µg/L threshold, 62-302.531(2)(a)(2)(b)1., F.A.C.

Dominant or co-dominant species were present in only 12 of the 72 samples taken to examine

whether a bloom condition existed at the time of collection. The cyanobacteria species (blue-

green algae) identified in those samples, Cylindrospermopsis rackiborskii, Planktolyngbya

contorta, Planktolyngbya limnetica, and Microcystis aeruginosa, have the potential to produce

toxins however, cyanotoxins were not detected in the 16 samples analyzed. Although potential

toxin producing species may have been present, laboratory results indicate that these species

were not producing toxins at the time of collection. Although algal density increased over time,

the phytoplankton community composition indicated no shifts in relative proportions of major

groups of algae over the study period.

The pattern of changes in both measured field parameters and water quality data, reflect the

pattern of average monthly rainfall received in Highlands County. Precipitation data was

retrieved for Highlands County from the Community Collaborative Rain, Hail & Snow Network

(CoCoRaHS) Rainy Day’s Report, https://www.cocorahs.org/ViewData/RainyDaysReport.aspx.

Both minimum and maximum precipitation were averaged by month for the period July 1, 2017

to June 30, 2017 (Figure 18). Precipitation data recorded from the station located on the

northeast shore of the lake, Sylvan Shores 8.5NE, # FL-HL-12, reflected the pattern in the

monthly county data. Increases in total suspended solids, turbidity, total Kjeldahl nitrogen, and

chlorophyll a along with decreases in color and total organic carbon coincide with periods of low

precipitation. This suggests that Lake Istokpoga’s water quality and algal community is

susceptible to, influenced by, and changes in response to weather-driven patterns.

https://www.cocorahs.org/ViewData/RainyDaysReport.aspx


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Table 1. Measured field parameters.

Field parameters measured at 6 locations on Lake Istokpoga on 12 sampling dates. A dash (-) indicates sample/measurement was not collected. The c indicates a

calculated value.

Site Parameter Unit 2016-

7-25

2016-

8-23

2016-

9-27

2016-

10-19

2016-

11-29

2016-

12-27

2017-

1-30

2017-

2-28

2017-

3-29

2017-

4-11

2017-

5-23

2017-

6-29

Center Temperature C 29.9 31.1 28.8 25.5 20.3 22.1 16.5 22.2 23.3 21.3 28.1 29.5

Center pH SU 7.9 7.8 7.1 7.4 7.0 7.2 7.6 7.9 8.3 8.8 8.8 8.0

Center Dissolved

Oxygen %Sat 101.7 c 83.4 101.8 103.8 c 113.5 108.8 99.4 c 106.7 108.3 116.3 c 101.1 98.5

Center Dissolved

Oxygen mg/L 7.7 - - 8.5 - - 9.7 - - 10.3 - -

Center Specific

Conductance

µmhos/

cm 101 - - 104 - - 130 138 - 117 - 189.2

Northeast Temperature C 29.8 30.8 29.4 25.4 20.3 22.0 16.6 22.6 24.1 21.3 28.4 29.2

Northeast pH SU 6.5 6.7 6.6 7.2 7.1 7.3 7.5 7.9 8.1 8.1 8.0 6.7

Northeast Dissolved

Oxygen %Sat 75.2 c 66.9 74.2 96.3 c 116.4 110.1 93.4 c 100.3 103.9 110.6 c 83.3 46.1

Northeast Dissolved

Oxygen mg/L 5.7 - - 7.9 - - 9.1 - - 9.8 - -

Northeast Specific

Conductance

µmhos/

cm 110 - - 103 - - 133 140 - 199 - 162.6

Northwest Temperature C 29.9 31.4 29.1 25.6 20.7 22.7 16.9 23.0 23.4 22.9 28.6 30.5

Northwest pH SU 8.2 7.9 7.3 7.3 6.6 7.9 7.9 8.5 7.9 8.8 8.5 8.6

Northwest Dissolved

Oxygen %Sat 111.0 c 115.3 87.4 93.0 c 114.9 100.8 90.9 c 115.8 99.3 117.6 c 89.1 101.3

Northwest Dissolved

Oxygen mg/L 8.4 - - 7.6 - - 8.8 - - 10.1 - -

Northwest Specific

Conductance

µmhos/

cm 107 - - 104 - - 133 139 - 198 - 170.8

South Temperature C 29.9 30.9 30.0 26.0 20.3 22.1 16.7 22.8 24.8 22.2 28.2 30.6


Page 12 of 96

Site Parameter Unit 2016-

7-25

2016-

8-23

2016-

9-27

2016-

10-19

2016-

11-29

2016-

12-27

2017-

1-30

2017-

2-28

2017-

3-29

2017-

4-11

2017-

5-23

2017-

6-29

South pH SU 8.2 7.4 7.1 7.7 6.8 7.2 7.6 8.1 8.5 8.7 7.8 8.7

South Dissolved

Oxygen %Sat 100.4 c 94.5 74.8 108.5 c 103.1 106.2 89.5 c 101.8 113.3 114.8 c 70.6 96.3

South Specific

Conductance

µmhos/

cm 116 - - 106 - - 127 134 - 116 - 160.3

Southeast Temperature C 29.6 31.2 29.2 25.4 20.4 22.1 16.5 22.3 24.4 21.2 27.8 30.4

Southeast pH SU 7.8 7.0 6.9 7.6 6.9 7.2 7.7 8.0 8.2 8.6 9.3 8.6

Southeast Dissolved

Oxygen %Sat 95.9 c 81.8 77.6 102.4 c 112.0 106.6 87.1 c 65.7 110.8 105.9 c 102.3 95.7

Southeast Dissolved

Oxygen mg/L 7.3 - - 8.4 - - 8.5 - - 9.4 - -

Southeast Specific

Conductance

µmhos/

cm 107 - - 106 - - 128 136 - 192 - 163.8

West Temperature C 29.5 31.4 28.7 25.4 20.1 21.8 16.5 22.8 23.8 23.3 28.0 29.7

West pH SU 8.4 8.3 7.4 7.4 6.9 7.5 8.6 8.4 9.1 9.3 8.2 9.2

West Dissolved

Oxygen %Sat 102.3 c 116.4 96.7 102.4 c 114.2 104.5 96.3 c 109.7 116.5 124.3 c 89.1 112.5

West Dissolved

Oxygen mg/L 7.8 - - 8.4 - - 9.4 - - 10.6 - -

West Specific

Conductance

µmhos/

cm 108 - - 105 - - 129 135 - 194 - 174.5


Page 13 of 96

Figure 2. Graph of water temperature.

Figure 3. Graph of pH.

14

16

18

20

22

24

26

28

30

32

34

°C

Sample Date

Temperature

Center

Northeast

Northwest

South

Southeast

West

5

6

7

8

9

10

SU

Sample Date

pH

Center

Northeast

Northwest

South

Southeast

West


Page 14 of 96

Figure 4. Graph of dissolved oxygen.

Figure 5. Graph of specific conductance.

40

50

60

70

80

90

100

110

120

130

140

% s

atura

tion

Sample Date

Dissolved Oxygen

Center

Northeast

Northwest

South

Southeast

West

60

80

100

120

140

160

180

200

220

µm

hos/

cm

Sample Date

Specific Conductance

Center

Northeast

Northwes

tSouth

Southeast


Page 15 of 96

Table 2. Laboratory analytical results.

Analytical results for 6 locations on Lake Istokpoga on 12 sampling dates. Data qualifiers are as follows: A - Value reported is the mean of two or more

determinations; I - The reported value is between the laboratory method detection limit and the laboratory practical quantitation limit; J – Estimated value; Q -

Sample held beyond normal holding time; U - Material analyzed for but not detected; value reported is the method detection limit.

Site Analyte Unit 2016-

7-25

2016-

8-23

2016-

9-27

2016-

10-19

2016-

11-29

2016-

12-27

2017-

1-30

2017-

2-28

2017-

3-29

2017-

4-11

2017-

5-23

2017-

6-29

Center Total Ammonia mg/L 0.009 0.007 0.006 0.004 I 0.006 0.009 0.005 I 0.0078 0.004 I 0.004 I 0.007 0.008

Center Chlorophyll a,

Corrected µg/L 33 30 34 34 48 38 J 56 42 49 51 A 63 48

Center Color (true) PCU 160 120 110 120 Q 130 160 88 75 A 65 67 58 88

Center Total Kjeldahl

Nitrogen mg/L 1.1 J 1.2 1.2 1.2 1.4 1.2 1.3 1.5 1.2 1.7 1.7 1.8

Center Nitrate -Nitrite mg/L 0.004

U

0.004

U

0.004

U

0.004

U

0.004

U

0.004

U

0.004

U

0.004

U

0.004

U

0.004

U

0.004

U

0.004

U

Center Ortho-phosphate mg/L 0.004

U

0.004

U

0.004

U

0.008

IQ

0.004

U

0.004

U 0.004 I

0.004

U

0.004

U

0.004

U

0.004

U

0.004

U

Center Total Organic

Carbon mg/L 17 15 15 17 16 16 15 15 15 15 14 16

Center Total

Phosphorus mg/L 0.075 0.059 0.055 0.068 0.059 0.067 0.13 0.083 0.08 0.095 0.097 0.1

Center Total Suspended

Solids mg/L 13 I 9 I 9 I 8 I 12 6 I 24 20 24 I 24 I 42 I 17 I

Center Turbidity NTU 6.9 4.3 A 4.6 5.9 Q 7.1 5.2 14 8.3 9 11 10 8.8

Northeast Total Ammonia mg/L 0.009 0.005 0.006 0.004 I 0.005 0.008 0.005 I 0.006 0.004 I 0.004 I 0.006 0.06

Northeast Chlorophyll a,

Corrected µg/L 26 32 33 A 25 23 19 J 55 A 40 44 44 66 18

Northeast Color (true) PCU 190 130 110 160 Q 190 190 91 74 65 67 57 320

Northeast Total Kjeldahl

Nitrogen mg/L 0.84 1.2 1.2 1.1 0.99 1.1 1.3 1.4 1.3 2.2 2 1.7


Page 16 of 96


7-25

2016-

8-23

2016-

9-27

2016-

10-19

2016-

11-29

2016-

12-27

2017-

1-30

2017-

2-28

2017-

3-29

2017-

4-11

2017-

5-23

2017-

6-29

Northeast Nitrate -Nitrite mg/L 0.004

U

0.004

U

0.004

U

0.004

U 0.009 I

0.004

U 0.007 I

0.004

U

0.004

U

0.004

U

0.004

U 0.009 I

Northeast Ortho-phosphate mg/L 0.016 0.004

U

0.004

U 0.05 Q 0.036 0.037 0.004 I

0.004

U

0.004

U

0.004

U

0.004

U 0.14

Northeast Total Organic

Carbon mg/L 19 15 15 19 19 18 15 14 15 14 14 27

Northeast Total

Phosphorus mg/L 0.07 0.066 0.055 0.1 0.26 0.092 0.12 0.072 0.075 0.092 0.12 0.24

Northeast Total Suspended

Solids mg/L 6 I 12 8 I 10 A 4 I 4 I 26 17 19 20 I 48 I 7 I

Northeast Turbidity NTU 3.7 4.7 4.9 A 2.9 Q 2.6 2.9 15 7.5 7.4 9.4 A 15 3.5

Northwest Total Ammonia mg/L 0.007 0.007 0.006 0.003 I 0.005 I 0.007 0.005 I 0.01 0.004 I 0.004 I 0.006 0.008

Northwest Chlorophyll a,

Corrected µg/L 29 46 49 45 34 34 J 54 26 AQ 63 54 72 6.4

Northwest Color (true) PCU 170 110 100 130 Q 130 130 85 75 Q 65 68 57 51

Northwest Total Kjeldahl

Nitrogen mg/L 0.78 1.3 1.1 1.2 1.2 1.1 1.4 1.6 1.5 2 1.6 1.8

Northwest Nitrate -Nitrite mg/L 0.004

U 0.1

0.004

U

0.004

U 0.004 I 0.008 I

0.004

U

0.004

U

0.004

U

0.004

U

0.004

U

0.004

U

Northwest Ortho-phosphate mg/L 0.008 I 0.004 I 0.005 I 0.004

UQ

0.004

U

0.004

U

0.004

U

0.004

UQ

0.004

U

0.004

U

0.004

U

0.004

U

Northwest Total Organic

Carbon mg/L 18 15 15 15 16 16 15 14 15 15 14 13

Northwest Total

Phosphorus mg/L 0.075 0.062 0.058 0.058 0.056 0.05 0.082 0.074 0.086 0.1 0.098 0.078

Northwest Total Suspended

Solids mg/L 9 I 12 17 11 9 I 10 22 24 26 23 I 46 I 40 I

Northwest Turbidity NTU 5.9 5.7 9.1 6.3 Q 5.8 6.4 12 8.8 AQ 10 12 12 9.4

South Total Ammonia mg/L 0.009 0.006 0.006 0.004 I 0.006 0.008 0.004 I 0.007 0.005 I 0.004 I 0.008 0.007


Page 17 of 96


7-25

2016-

8-23

2016-

9-27

2016-

10-19

2016-

11-29

2016-

12-27

2017-

1-30

2017-

2-28

2017-

3-29

2017-

4-11

2017-

5-23

2017-

6-29

South Chlorophyll a,

Corrected µg/L 42 34 40 38 40 14 J 16 54 43 45 60 51 A

South Color (true) PCU 97 A 110 99 83 80 71 85 74 67 A 66 55 43

South Total Kjeldahl

Nitrogen mg/L 1.6 1.2 1.3 1.4 1.6 1.2 1.3 1.7 1.4 1.9 2.2 2.1

South Nitrate -Nitrite mg/L 0.004

U

0.004

U

0.004

U

0.004

U 0.004 I

0.004

U

0.004

U

0.004

U

0.004

U

0.004

U

0.004

U

0.004

U

South Ortho-phosphate mg/L 0.004

U

0.004

U

0.004

U

0.004

U

0.004

U

0.004

U

0.004

U

0.004

U

0.004

U

0.004

U

0.004

U

0.004

U

South Total Organic

Carbon mg/L 14 15 15 15 15 14 16 15 16 15 14 13

South Total

Phosphorus mg/L 0.086 0.059 0.059 0.06 0.03 0.053 0.1 0.079 0.08 0.11 0.11 0.084

South Total Suspended

Solids mg/L 16 11 12 15 I 17 6 I 29 20 21 I 21 I 44 I 40 I

South Turbidity NTU 9.3 4.8 5.8 6.8 6.6 5.8 14 9.3 7.7 A 12 12 9.9

Southeast Total Ammonia mg/L 0.008 0.006 0.005 0.004 I 0.006 0.009 0.005 0.006 0.005 I 0.004 I 0.009 0.008

Southeast Chlorophyll a,

Corrected µg/L 37 33 36 38 42 30 66 53 38 41 54 45

Southeast Color (true) PCU 110 120 100 120 A 110 110 A 86 74 64 67 A 59 44

Southeast Total Kjeldahl

Nitrogen mg/L 1.5 1.2 1.1 1.3 1.4 1.2 1.6 1.7 1.3 1.8 2 1.5

Southeast Nitrate -Nitrite mg/L 0.004

U

0.004

U

0.004

U

0.004

U

0.004

U

0.004

U

0.004

U

0.004

U 0.004 I

0.004

U

0.004

U

0.004

U

Southeast Ortho-phosphate mg/L 0.004

U

0.004

U

0.004

U

0.004

UQ

0.004

U

0.004

U 0.005 I

0.004

U 0.006 I

0.004

U

0.004

U

0.004

U

Southeast Total Organic

Carbon mg/L 15 15 15 16 16 16 15 15 15 16 16 14

Southeast Total

Phosphorus mg/L 0.083 0.056 0.066 0.073 0.064 0.061 0.13 0.08 0.072 0.099 0.11 0.077


Page 18 of 96


7-25

2016-

8-23

2016-

9-27

2016-

10-19

2016-

11-29

2016-

12-27

2017-

1-30

2017-

2-28

2017-

3-29

2017-

4-11

2017-

5-23

2017-

6-29

Southeast Total Suspended

Solids mg/L 18 12 13 12 12 9 I 25 19 21 I 22 I 46 I 38 I

Southeast Turbidity NTU 7.9 5 5.4 6.5 6.3 5.9 14 9.4 7.1 10 12 8.9 A

West Total Ammonia mg/L 0.007 0.007 0.006 0.005 0.006 0.011 0.005 I 0.009 0.004 I 0.005 I 0.007 0.008

West Chlorophyll a,

Corrected µg/L 25 29 35 46 Q 53 38 AJ 50 60 Q 49 48 62 A 63

West Color (true) PCU 140 120 110 A 100 Q 110 110 85 76 Q 66 69 56 65

West Total Kjeldahl

Nitrogen mg/L 1.2 1.2 1.1 1.6 1.4 1.3 1.3 1.4 1.7 1.8 1.9 1.9

West Nitrate -Nitrite mg/L 0.004

U

0.004

U

0.004

U

0.004

U

0.004

U

0.004

U

0.004

U

0.004

U

0.004

U

0.004

U

0.004

U

0.004

U

West Ortho-phosphate mg/L 0.004

U

0.004

U

0.004

U

0.004

UQ

0.004

U

0.004

U 0.004 I

0.004

UQ

0.004

U

0.004

U

0.004

U

0.004

U

West Total Organic

Carbon mg/L 16 15 15 15 16 14 15 14 16 16 14 14

West Total

Phosphorus mg/L 0.067 0.055 0.061 0.072 0.055 0.055 0.11 0.085 0.083 0.1 0.1 0.096

West Total Suspended

Solids mg/L 12 13 12 13 I 12 10 I 29 35 24 I 22 I 48 I 38 I

West Turbidity NTU 6.6 4.5 5.2 8.1 Q 6.5 A 6.2 13 9.9 Q 9.8 11 12 A 10


Page 19 of 96

Figure 6. Graph of color (true).

Figure 7. Graph of total organic carbon.

0

50

100

150

200

250

300

350

PC

U

Sample Date

Color (true)

Center

Northeast

Northwest

South

Southeast

West

0

5

10

15

20

25

30

mg C

/L

Sample Date

Total Organic Carbon

Center

Northeast

Northwest

South

Southeast

West


Page 20 of 96

Figure 8. Graph of total suspended solids.

Figure 9. Graph of turbidity.

0

10

20

30

40

50

60

mg/L

Sample Date

Total Suspended Solids

Center

Northeast

Northwest

South

Southeast

West

0

2

4

6

8

10

12

14

16

NT

U

Sample Date

Turbidity

Center

Northeast

Northwest

South

Southeast

West


Page 21 of 96

Figure 10. Graph of ortho-phosphate.

Figure 11. Graph of total phosphorus.

0

0.02

0.04

0.06

0.08

0.1

0.12

0.14

0.16

mg/L

Sample Date

Ortho-phosphate

Center

Northeast

Northwest

South

Southeast

West

0

0.05

0.1

0.15

0.2

0.25

0.3

mg/L

Sample Date

Total Phosphorus

Center

Northeast

Northwest

South

Southeast

West


Page 22 of 96

Figure 12. Graph of total Kjeldahl nitrogen.

Figure 13. Graph of ammonia.

0

0.5

1

1.5

2

2.5

mg/L

Sample Date

Total Kjeldahl Nitrogen

Center

Northeast

Northwest

South

Southeast

West

0

0.01

0.02

0.03

0.04

0.05

0.06

0.07

mg/L

Sample Date

Ammonia

Center

Northeast

Northwest

South

Southeast

West


Page 23 of 96

Figure 14. Graph of nitrate-nitrite.

Figure 15. Graph of chlorophyll a, corrected.

0

0.02

0.04

0.06

0.08

0.1

0.12

mg/L

Sample Date

Nitrate-nitrite

Center

Northeast

Northwest

South

Southeast

West

0

10

20

30

40

50

60

70

80

µg/L

Sample Date

Chlorophyll a, corrected

Center

Northeast

Northwest

South

Southeast

West


Page 24 of 96

Figure 16. Phytoplankton density.

Figure 17. Comparison of phytoplankton density to chlorophyll a, corrected.

0

20,000

40,000

60,000

80,000

100,000

120,000

140,000

160,000# u

nit

s/m

L

Sample Date

Phytoplankton Density

Center

Northeast

Northwest

South

Southeast

West

0

10

20

30

40

50

60

70

80

0 20,000 40,000 60,000 80,000 100,000 120,000 140,000 160,000

Chlo

rophyll

a, co

rrec

ted (

µg/L

)

Phytoplankton Sample Density (# algal units/mL)

Comparison of Chlorophyll a to Phytoplankton Density

Center

Northeast

Northwest

South

Southeast

West


Page 25 of 96

Table 3. Dominance of algal species.

The presence of dominant or co-dominant algal species in water samples collected at 6 locations on Lake Istokpoga on 12 sampling dates to determine if a bloom

condition was affecting the waterbody at time of collection. The table contains the count of samples and sites for each result. Abbreviations used within the table

are C-Central, NE-Northeast, NW-Northwest, SE-Southeast, S-South, W-West.

Dominant Taxon 2016-

7-25

2016-

8-23

2016-

9-27

2016-

10-19

2016-

11-16

2016-

12-27

2017-

1-30

2017-

2-28

2017-

3-29

2017-

4-11

2017-

5-23

2017-

6-29

None; mixed assemblage 1-NE 6 6 1-NE 6 6 6 6 5 6 5 6

Cylindrospermopsis rackiborskii. - - - - - - - - - - 1-W -

Planktolyngbya contorta. 2-C,

NW - - 1-C - - - - - - - -

Planktolyngbya contorta and

Microcystis aeruginosa.

2-SE,

W - - 1-SE - - - - - - - -


Cylindrospermopsis raciborskii. 1-S - - 1-W - - - - - - - -


Planktolyngbya limnetica. - - -

2-NW,

S - - - - 1-S - - -


Page 26 of 96

Table 4. Phytoplankton taxonomic variables.

Summary of phytoplankton in quantitative water samples collected at 6 locations on Lake Istokpoga on 12 sampling dates. The table contains the count of

samples and sites for each result.

Site Variable 2016-

7-25

2016-

8-23

2016-

9-27

2016-

10-19

2016-

11-29

2016-

12-27

2017-

1-30

2017-

2-28

2017-

3-29

2017-

4-11

2017-

5-23

2017-

6-29

Center Number of Wet Taxa 34 37 45 47 39 47 36 33 30 42 30 50

Center Number Wet Algal

Units Identified

303 305 307 366 301 303 336 306 306 319 313 301

Center Algal Density

(# units/mL)

39,214 30,129 21,571 44,229 24,749 56,826 74,351 39,967 48,494 124,670 772,21 74,328

Center Percent Dominant

Wet Taxon

42.74 15.85 10.27 23.4 12.56 21.13 40.72 12.2 12.92 41.28 12.5 13.01

Center % Blue-green 63.37 65.9 48.86 51.9 53.49 66.67 68.75 62.42 71.24 74.92 74.76 68.44

Center % Green 12.86 20.98 22.8 25.4 20.27 20.46 22.02 20.59 17.32 18.18 14.38 20.93

Center % Yellow-green 0 0 0.33 0.8 0 0.33 0 0 0 0 0 0

Center % Cryptophytes 0 0 0.33 0.7 0 0 0.31 0 0 0.31 0 0.33

Center % Euglenophytes 0.33 0 0.65 0.4 0 0 0 0 0 0 0 0

Center % Pyrrophytes 0.66 0 0.33 4.9 0.33 0 0 0 0 0 0.32 0.32

Center % Diatoms 22.78 13.11 27.04 21.9 25.91 12.54 9.23 16.99 11.44 6.58 10.54 10.63

Northeast Number of Wet Taxa 38 50 44 46 76 99 36 35 33 37 34 53

Northeast Number Wet Algal

Units Identified

304 311 302 192 604 534 325 301 303 304 312 301

Northeast Algal Density

(# units/mL)

3,158 4,800 7093 22,178 44,144 88,858 84,072 35,211 33,616 108,272 69,153 22,291

Northeast Percent Dominant

Wet Taxon

47.09 10.89 17.06 22.75 7.47 11.28 38.54 11.6 12.69 36.39 14.29 11.31

Northeast % Blue-green 15.77 43.09 51.66 55.2 41.39 67.42 62.15 55.81 68.65 78.29 60.26 62.79

Northeast % Green 13.33 29.9 27.48 30.2 22.85 20.22 26.15 27.24 19.8 17.76 18.27 27.91


Page 27 of 96

Site Variable 2016-

7-25

2016-

8-23

2016-

9-27

2016-

10-19

2016-

11-29

2016-

12-27

2017-

1-30

2017-

2-28

2017-

3-29

2017-

4-11

2017-

5-23

2017-

6-29

Northeast % Yellow-green 0.32 0 0.33 0.1 0 0.33 0 0 0 0.33 0 0

Northeast % Cryptophytes 0 0.32 0.33 0.0 0.99 0.67 0.31 0 0 0.31 0 0.33

Northeast % Euglenophytes 0.32 2.25 0.33 0.2 0 0 0 0 0 0 0 0

Northeast % Pyrrophytes 26.73 7.07 3.31 1.0 0.33 0 0 0 0 0 0 0.32

Northeast % Diatoms 43.54 17.36 16.56 13.5 35.76 11.99 11.38 16.94 11.55 3.62 21.47 8.97

Northwest Number of Wet Taxa 25 40 45 47 38 47 41 41 40 39 32 44

Northwest Number Wet Algal

Units Identified

303 305 304 262 303 314 308 302 310 355 318 304

Northwest Algal Density

(# units/mL)

37,737 29,822 23,004 32,151 21,547 67,403 73,296 41,088 56,423 149,873 77,531 91,489

Northwest Percent Dominant

Wet Taxon

50 15.13 8.43 22.27 14.15 19.09 38.14 15.51 10.75 44.38 16.8 15.07

Northwest % Blue-green 52.14 65.9 53.62 52.3 50.17 52.87 70.13 55.63 63.23 77.18 58.49 71.71

Northwest % Green 8.59 22.62 31.58 40.5 18.81 17.2 24.03 25.5 26.77 17.75 20.13 17.43

Northwest % Yellow-green 0 0 0.33 0.4 0 0 0 0 0 0.33 0 0

Northwest % Cryptophytes 0 0 0.33 0.0 0.99 0 0.31 0 0 0.31 0 0.33

Northwest % Euglenophytes 0 0.33 0.33 0.4 0 0 0 0 0 0 0 0

Northwest % Pyrrophytes 0 0 0.33 0.3 0.33 0 0 0 0 0 0.32 0.32

Northwest % Diatoms 39.27 11.15 14.14 6.1 30.03 29.94 5.84 18.87 10 4.79 21.38 10.53

South Number of Wet Taxa 32 39 48 44 40 51 40 38 37 40 32 38

South Number Wet Algal

Units Identified

303 306 301 344 308 300 342 312 301 332 305 298

South Algal Density

(# units/mL)

37,308 53,752 20,385 70,356 28,699 63,603 131,672 40,339 33,975 140,199 55,130 73,286

South Percent Dominant

Wet Taxon

42.74 16.41 11.37 25.84 12.35 23.26 39.61 17.18 10.8 43.25 14.23 12.45


Page 28 of 96

Site Variable 2016-

7-25

2016-

8-23

2016-

9-27

2016-

10-19

2016-

11-29

2016-

12-27

2017-

1-30

2017-

2-28

2017-

3-29

2017-

4-11

2017-

5-23

2017-

6-29

South % Blue-green 64.03 54.9 40.86 62.5 57.47 63 68.13 62.18 70.1 74.7 69.51 72.82

South % Green 12.87 28.43 43.19 32.3 21.43 22 21.35 21.79 25.25 23.49 18.03 18.79

South % Yellow-green 0 0 0 0.1 0 0.33 0 0 0 0 0 0

South % Cryptophytes 0 0.33 0 0.0 0.99 0.67 0.31 0 0 0 0 0.33

South % Euglenophytes 0 0 0 0.0 0 0 0 0 0 0 0 0

South % Pyrrophytes 0.33 0 0.66 0.3 0.33 0 0 0 0 0 0.32 0.32

South % Diatoms 22.77 16.34 15.28 4.4 21.1 14 9.94 16.03 4.65 1.81 12.46 8.39

Southeast Number of Wet Taxa 21 40 48 47 45 39 31 33 40 39 37 37

Southeast Number Wet Algal

Units Identified

303 311 309 377 312 301 315 305 309 317 303 304

Southeast Algal Density

(# units/mL)

37,516 41,399 22,889 74,761 26,693 60,391 112,026 41,134 43,246 122,058 44,119 90,331

Southeast Percent Dominant

Wet Taxon

48.39 13.72 10.91 24.36 12.39 37.32 40.82 18.11 12.5 40.06 16.93 15.5

Southeast % Blue-green 79.53 60.13 53.72 63.4 51.92 77.41 68.57 61.97 65.37 77.92 51.82 64.47

Southeast % Green 12.22 28.94 34.95 30.2 19.55 14.29 24.76 24.92 20.06 20.5 32.01 20.39

Southeast % Yellow-green 0 0 0 0.2 0 0.33 0 0 0 0 0 0

Southeast % Cryptophytes 0 0 0 0.0 0.32 0.67 0.31 0 0 0 0 0.33

Southeast % Euglenophytes 0.33 0 0 0.4 0.32 0 0 0 0 0 0 0

Southeast % Pyrrophytes 0 0 0.32 3.9 0.32 0 0 0 0 0 0.32 0.32

Southeast % Diatoms 7.92 10.93 11 6.4 27.56 8.31 6.67 13.11 14.56 1.58 16.17 15.13

West Number of Wet Taxa 25 39 45 53 37 36 30 34 69 38 31 41

West Number Wet Algal

Units Identified

303 312 301 339 303 307 322 308 611 315 306 300


Page 29 of 96

Site Variable 2016-

7-25

2016-

8-23

2016-

9-27

2016-

10-19

2016-

11-29

2016-

12-27

2017-

1-30

2017-

2-28

2017-

3-29

2017-

4-11

2017-

5-23

2017-

6-29

West Algal Density

(# units/mL)

43,688 35,887 31,508 49,683 24,750 95,641 88,437 49,340 108,481 130,930 54,573 109,613

West Percent Dominant

Wet Taxon

53.66 16.31 10.61 22.22 14.4 25.56 39.87 16 7.13 49.18 20.15 24.71

West % Blue-green 73.27 68.91 55.48 53.1 58.75 74.59 71.74 50.97 78.23 80.32 68.3 69

West % Green 7.92 21.47 25.58 36.9 23.76 11.73 22.98 21.75 18.17 16.19 17.65 18.67

West % Yellow-green 0 0 0.33 0.1 0 0.33 0 0 0 0.33 0 0

West % Cryptophytes 0 0 0.33 0.1 0 0 0.31 0 0 0.31 0 0.33

West % Euglenophytes 0 0 0.33 0.0 0 0 0 0.32 0 0 0 0

West % Pyrrophytes 0 0 0.33 0.2 0.33 0 0 0 0 0 0.32 0.32

West % Diatoms 18.81 9.62 18.6 10.0 17.49 13.36 5.28 26.95 3.6 3.49 14.05 12.33


Page 30 of 96

Figure 18. Box and whisker plot of the percent composition by algal group.


Page 31 of 96

Table 5. Dominant wet and diatom taxa.

Dominant wet and diatom taxa in quantitative phytoplankton samples for 6 locations on Lake Istokpoga on 12 sampling dates.

Date Variable Center Northeast Northwest Southeast South West

2016-

7-25

Dominant Wet

Taxon Name

Planktolyngbya

contorta

Glenodinium sp. Planktolyngbya

contorta

Planktolyngbya

contorta

Planktolyngbya

contorta

Planktolyngbya

contorta

2016-

7-25

Class Cyanophyceae Dinophyceae Cyanophyceae Cyanophyceae Cyanophyceae Cyanophyceae

2016-

8-23

Dominant Wet

Taxon Name

Planktolyngbya

contorta

Dolichospermum

circinale

Planktolyngbya

contorta

Snowella sp. Snowella sp. Planktolyngbya

contorta

2016-

8-23

Class Cyanophyceae Cyanophyceae Cyanophyceae Cyanophyceae Cyanophyceae Cyanophyceae

2016-

9-27

Dominant Wet

Taxon Name

Planktolyngbya

contorta

Aphanizomenon

flosaquae

Planktolyngbya

contorta

Dictyosphaerium

pulchellum

Dictyosphaerium

pulchellum

Snowella sp.

2016-

9-27


2016-

10-19

Dominant Wet

Taxon Name

Cyanodictyon

imperfectum

Cyanodictyon

imperfectum

Cyanodictyon

imperfectum

Cyanodictyon

imperfectum

Cyanodictyon

imperfectum

Cyanodictyon

imperfectum

2016-

10-19


2016-

11-29

Dominant Wet

Taxon Name

Aphanocapsa incerta Planktolyngbya

limnetica

Aphanocapsa

delicatissima

Snowella sp. Aphanocapsa

delicatissima

Aphanocapsa

delicatissima

2016-

11-29


2016-

12-27

Dominant Wet

Taxon Name

Cyanodictyon

imperfectum

Cyanodictyon

imperfectum

Cyanodictyon

imperfectum

Cyanodictyon

imperfectum

Cyanodictyon

imperfectum

Cyanodictyon sp.

2016-

12-27


2017-

1-30

Dominant Wet

Taxon Name

Cyanodictyon

imperfectum

Cyanodictyon

imperfectum

Cyanodictyon

imperfectum

Cyanodictyon

imperfectum

Cyanodictyon

imperfectum

Cyanodictyon

imperfectum

2017-

1-30



Page 32 of 96


2017-

2-28

Dominant Wet

Taxon Name

Cylindrospermopsis

raciborskii

Aphanocapsa

delicatissima

Cylindrospermopsis

raciborskii

Cylindrospermopsis

raciborskii

Cylindrospermopsis

raciborskii

Cylindrospermopsis

raciborskii

2017-

2-28


2017-

3-29

Dominant Wet

Taxon Name

Snowella sp. Aphanocapsa

delicatissima

Cyanodictyon sp. Cyanodictyon sp. Planktolyngbya

contorta

Planktolyngbya

contorta

2017-

3-29


2017-

4-11

Dominant Wet

Taxon Name

Cyanodictyon

imperfectum

Cyanodictyon

imperfectum

Cyanodictyon

imperfectum

Cyanodictyon

imperfectum

Cyanodictyon

imperfectum

Cyanodictyon

imperfectum

2017-

4-11


2017-

5-23

Dominant Wet

Taxon Name

Cylindrospermopsis

raciborskii

Merismopedia

warmingiana

Cylindrospermopsis

raciborskii

Dictyosphaerium

pulchellum

Snowella sp. Cylindrospermopsis

raciborskii

2017-

5-23


2017-

6-29

Dominant Wet

Taxon Name

Cyanodictyon

imperfectum

Cyanodictyon

imperfectum

Aphanothece sp. Planktolyngbya

contorta

Cyanodictyon

imperfectum

Cylindrospermopsis

raciborskii

2017-

6-29


2016-

7-25

Dominant

Diatom Taxon

Name

Aulacoseira

granulata

Staurosirella pinnata Staurosira

construens

Staurosira

construens

Staurosira

construens

Staurosira

construens

2016-

8-23

Dominant

Diatom Taxon

Name

Staurosira

construens

Aulacoseira sp. Staurosira

construens

Aulacoseira

granulata

Staurosira

construens

Staurosira

construens

2016-

9-27

Dominant

Diatom Taxon

Name

Aulacoseira

granulata


construens


construens

Staurosira

construens

2016-

10-19

Dominant

Diatom Taxon

Name

Aulacoseira

granulata

Aulacoseira

granulata

Aulacoseira

granulata

Aulacoseira

granulata

Staurosira

construens

Aulacoseira

granulata


Page 33 of 96


2016-

12-27

Dominant

Diatom Taxon

Name

Aulacoseira

granulata

Aulacoseira

granulata

Aulacoseira

granulata

Staurosira

construens

Aulacoseira

granulata

Aulacoseira

granulata

2017-

1-30

Dominant

Diatom Taxon

Name

Aulacoseira

granulata

Aulacoseira

granulata

Aulacoseira

granulata

Aulacoseira

granulata

Aulacoseira

granulata

Staurosira

construens

2016-

11-29

Dominant

Diatom Taxon

Name

Aulacoseira

granulata

Aulacoseira

granulata

Aulacoseira

granulata

Aulacoseira

granulata

Aulacoseira

granulata

Aulacoseira

granulata

2017-

2-28

Dominant

Diatom Taxon

Name

Staurosira

construens

Staurosirella pinnata Aulacoseira

granulata

Staurosira

construens

Staurosira

construens

Staurosira

construens

2017-

3-29

Dominant

Diatom Taxon

Name

Fragilaria

berolinensis

Staurosira

construens

Staurosira

construens

Staurosira

construens

Staurosira

construens

Staurosirella pinnata

2017-

4-11

Dominant

Diatom Taxon

Name

Staurosirella pinnata Staurosirella pinnata Fragilaria

berolinensis

Staurosira

construens

Staurosira

construens

Fragilaria

berolinensis

2017-

5-23

Dominant

Diatom Taxon

Name

Fragilaria

berolinensis

Staurosira

construens

Staurosirella pinnata Fragilaria

berolinensis

Fragilaria

berolinensis

Staurosira

construens

2017-

6-29

Dominant

Diatom Taxon

Name

Staurosira

construens

Staurosira

construens

Staurosira

construens

Staurosira

construens

Fragilaria

berolinensis

Staurosira

construens


Page 34 of 96

Figure 19. Average monthly minimum and maximum precipitation for Highlands County.

0.0

0.2

0.4

0.6

0.8

1.0

1.2

1.4

Aver

age

(inch

es)

Year-Month

Monthly Precipitation

Average of Min Average of Max


Page 35 of 96

Appendix A. Phytoplankton Wet Taxa

Site Taxon 2016-

7-25

2016-

8-23

2016-

9-27

2016-

10-19

2016-

11-29

2016-

12-27

2017-

1-30

2017-

2-28

2017-

3-29

2017-

4-11

2017-

5-23

2017-

6-29

Center Actinastrum hantzschii - - - - 1 1 - - - - - -

Center Ankistrodesmus - - - - - 8 - - - 1 - -

Center Ankistrodesmus falcatus 3 - 3 - 2 3 - - - - - -

Center Ankistrodesmus

fusiformis

- - - - 2 - - - 3 - - -

Center Aphanizomenon

flosaquae

2 6 5 - 4 1 - - 4 - - 1

Center Aphanocapsa 54 - - - - 8 2 - - 14 - 6

Center Aphanocapsa conferta - - - 7 - - 5 - - 5 - -

Center Aphanocapsa

delicatissima

1 14 10 7 23 8 21 19 25 2 25 15

Center Aphanocapsa elachista - - - - - - - - - 1 - -

Center Aphanocapsa incerta - 10 1 - 28 11 - 18 18 - 11 4

Center Aphanocapsa

planctonica

- - - 5 10 7 1 4 - 1 - -

Center Aphanothece - 12 - 1 - 10 1 9 10 2 25 20

Center Aphanothece clathrata - - - 12 4 - 14 - - 29 - 6

Center Aphanothece smithii - - - - - - - - - 1 - -

Center Bacillariophyta 69 40 83 80 78 38 29 52 35 21 33 32

Center Centritractus

belanophorus

- - - - - 1 - - - - - -


Page 36 of 96

Site Taxon 2016-

7-25

2016-

8-23

2016-

9-27

2016-

10-19

2016-

11-29

2016-

12-27

2017-

1-30

2017-

2-28

2017-

3-29

2017-

4-11

2017-

5-23

2017-

6-29

Center Chlamydomonas 1 2 3 - 4 3 - - - - 1 2

Center Chlorella - - - - - - - - - - - 1

Center Chlorophyceae - - - 30 - - 15 - - 20 - 5

Center Chodatella subsalsa - - - - - - 2 - - - - 1

Center Chroococcales - - - 31 - - - - - - - 4

Center Chroococcus - - - 1 - 7 - - - - - 17

Center Chroococcus dispersus - - 3 - 11 - 1 - - - - 7

Center Chroococcus minimus - - - - - - 6 - - 3 - -

Center Chroococcus minutus - - - - - - 1 - - - - -

Center Chroomonas - - 1 - - - - - - - - -

Center Closterium - 1 - - 1 - - - - - - -

Center Closterium acutum linea - - - - - - - - - 1 - -

Center Closterium acutum

variabile

- - - 1 - - - - - 1 - -

Center Closterium gracile - - - - - - - - - - 1 -

Center Coelastrum microporum - - - - - - - - - - - 1

Center Coelastrum

proboscideum

- - - - - 1 - - - - 1 -

Center Coenochloris fottii - - - 1 - - - - - - - -

Center Cosmarium - 1 - - - - - - - 1 - 1

Center Crucigenia apiculata - - - 1 2 - - - - - - -


Page 37 of 96

Site Taxon 2016-

7-25

2016-

8-23

2016-

9-27

2016-

10-19

2016-

11-29

2016-

12-27

2017-

1-30

2017-

2-28

2017-

3-29

2017-

4-11

2017-

5-23

2017-

6-29

Center Crucigenia crucifera - - - - - 1 - - - - - -

Center Crucigenia irregularis - - - - - - - 2 - - - -

Center Crucigenia rectangularis - 1 4 - - - - 1 - - - 2

Center Crucigenia tetrapedia - - 1 - - - - 1 - - - -

Center Cryptophyceae - - - - - - - - - 1 - -

Center Cyanodictyon - 8 7 - 9 - - 7 18 - 17 -

Center Cyanodictyon

imperfectum

- - - 67 - 56 125 - - 123 - 35

Center Cyanophyceae - - - 14 - 3 10 - - 10 - 1

Center Cylindrospermopsis

gangetica

- 3 2 - - - - - - - - 7

Center Cylindrospermopsis

raciborskii

2 11 8 6 - 14 12 31 25 8 35 17

Center Cylindrospermum - - 1 - - - - - - - - -

Center Dactylococcopsis

acicularis

- - - 16 - - - - - - - -

Center Dictyosphaerium - - - - - - 4 - - - - -

Center Dictyosphaerium

ehrenbergianum

3 - 5 - - - 1 - 7 - - -


pulchellum

- 19 13 2 4 4 - 8 3 - 23 4


tetrachotomum

- - - 1 - - - - - - - -


Page 38 of 96

Site Taxon 2016-

7-25

2016-

8-23

2016-

9-27

2016-

10-19

2016-

11-29

2016-

12-27

2017-

1-30

2017-

2-28

2017-

3-29

2017-

4-11

2017-

5-23

2017-

6-29

Center Dolichospermum - - - - 1 - - - - - - -

Center Dolichospermum

circinale

5 14 18 - 2 3 - 2 - - - 1


helicoideum

- - 1 - - - - - - - - -


planctonicum

2 1 - - - - - - - - - -


spiroides

- 2 - - - - - - - - - -

Center Elakatothrix gelatinosa 2 - 1 - - 2 - - - - - -

Center Elakatothrix viridis - - - 1 - - - - - - - -

Center Euastrum - 2 - - - - - - - - - -

Center Glaucospira - - - - - 1 - - - - - -

Center Glenodinium - - 1 - - - - - - - 1 -

Center Gloeocystis - - - - - - - - - - - 4

Center Goniochloris - - - 2 - - - - - - - -

Center Kirchneriella - 1 6 - 4 1 - 6 4 1 - 3

Center Kirchneriella contorta - - - - - 2 - - - - - -

Center Kirchneriella lunaris

irregularis

- - - - - - 1 - - - - -

Center Kirchneriella obesa - - - - - - - 1 - - - -

Center Koliella - - - - 6 - - 8 - - - 1


Page 39 of 96

Site Taxon 2016-

7-25

2016-

8-23

2016-

9-27

2016-

10-19

2016-

11-29

2016-

12-27

2017-

1-30

2017-

2-28

2017-

3-29

2017-

4-11

2017-

5-23

2017-

6-29

Center Koliella longiseta

f.variabilis

- - - 1 - - - - - - - -

Center Lagerheimia - - - 1 - - - - - - - -

Center Lepocinclis 1 - - - - - - - - - - -

Center Merismopedia punctata - - - 1 - - - - - - - 1

Center Merismopedia

tenuissima

1 5 5 1 - 4 5 2 10 10 4 -

Center Merismopedia

warmingiana

2 15 19 - 7 15 - 7 24 - 27 10

Center Micractinium pusillum - - - 1 - - - - - - - -

Center Microcystis - - - 2 - - - - - 1 - 1

Center Microcystis aeruginosa 1 - 2 - 2 - - 2 - - 2 -

Center Microcystis wesenbergii 5 5 3 - - 1 - - 2 - 3 1

Center Monoraphidium - 6 6 - 6 1 - 9 2 1 2 -

Center Monoraphidium

arcuatum

- - - 3 1 4 1 - - 1 - 5


circinale

- - - 4 - - 2 - - 1 - -


contortum

- - - 1 - - - - - 2 - -


griffithii

- - - - - - 10 - - 5 - -


irregulare

- - - 8 - - 15 - - 3 - -


Page 40 of 96

Site Taxon 2016-

7-25

2016-

8-23

2016-

9-27

2016-

10-19

2016-

11-29

2016-

12-27

2017-

1-30

2017-

2-28

2017-

3-29

2017-

4-11

2017-

5-23

2017-

6-29

Center Nephrochlamys rostrata - - - - - - - - - 1 - -

Center Nostocales - - - 3 - - 1 - - 1 - -

Center Oocystis - 4 2 - 3 2 - 3 3 1 2 7

Center Oocystis parva - - - 1 - - - - - - - -

Center Oocystis pusilla - - - 1 - - - - - - - -

Center Pandorina morum - - 1 - - - - - - - - -

Center Pediastrum - - - - - - - - - - - 1

Center Pediastrum duplex 1 1 - - - - - 2 - - - -

Center Pediastrum tetras - - 3 - 3 - 2 1 - - - 1

Center Peridinium 2 - - - 1 - - - - - - -

Center Phacus - - 2 - - - - - - - - -

Center Planktolyngbya - - - - - - - - - 1 - -

Center Planktolyngbya contorta 100 42 23 20 16 18 14 18 24 8 28 18

Center Planktolyngbya lacustris 1 - 2 - - - - - - - - -

Center Planktolyngbya

limnetica

6 18 12 4 16 23 5 30 19 18 28 12

Center Pseudanabaena 2 - - - 1 3 4 2 1 - - 2

Center Pseudanabaena

acicularis

1 - - - - - - - - - - -

Center Pseudodidymocystis - - - - - - 1 - - - - -

Center Pseudodidymocystis

fina

- - - 2 - - 3 - - 2 - -


Page 41 of 96

Site Taxon 2016-

7-25

2016-

8-23

2016-

9-27

2016-

10-19

2016-

11-29

2016-

12-27

2017-

1-30

2017-

2-28

2017-

3-29

2017-

4-11

2017-

5-23

2017-

6-29

Center Quadrigula chodati - - - - 2 - - 1 2 - - -

Center Quadrigula closteroides - - - 1 - - 1 - - 1 - -

Center Rhabdogloea 7 4 9 - 14 5 - 17 3 - 9 3

Center Romeria - 2 2 - - 3 - 2 - - 2 6

Center Romeria leopoliensis - - - 4 3 - 2 - - - - -

Center Scenedesmus - - - 9 - 7 15 - - 9 - 8

Center Scenedesmus abundans 4 7 6 2 - 2 1 - 5 1 2 4

Center Scenedesmus

acuminatus

1 - - - - - - - 2 - 2 -

Center Scenedesmus

acutiformis

- 5 - - - - - - 8 - 4 -

Center Scenedesmus bicaudatus 1 - 1 - - 2 - 3 - - 1 1

Center Scenedesmus bijuga 1 2 4 - 2 - - 1 - - - 1

Center Scenedesmus brevispina - - - - - 1 - - - - - -

Center Scenedesmus communis - - - - - - - - - 1 - -

Center Scenedesmus dimorphus 5 - - - - 2 - - - - - 1

Center Scenedesmus obliquus - - 4 - - - - - - - - -

Center Scenedesmus opoliensis - - - - 4 - - - - - - -

Center Scenedesmus perforatus - - 1 1 - 2 - - 1 - 1 -

Center Scenedesmus

quadricauda

9 10 1 2 10 9 - 15 10 - 2 5

Center Scenedesmus verrucosus - - - - - - - - 1 - - -


Page 42 of 96

Site Taxon 2016-

7-25

2016-

8-23

2016-

9-27

2016-

10-19

2016-

11-29

2016-

12-27

2017-

1-30

2017-

2-28

2017-

3-29

2017-

4-11

2017-

5-23

2017-

6-29

Center Schroederia judayi 3 - - - - - - - - - - -

Center Schroederia setigera - - - - - - - - - - - 2

Center Selenastrum - - - - - - - - 1 - - -

Center Snowella - 22 16 - 10 1 - 21 35 - 17 -

Center Snowella lacustris - - - 3 - - - - - 1 - 10

Center Snowella litoralis - - - 1 - - 1 - - - - 1

Center Spirulina - 1 1 - - - - - - - 1 -

Center Staurastrum 3 1 - - 1 1 - - 1 - 3 1

Center Staurodesmus - - - 1 - - - - - - - -

Center Stichococcus - - - - - 1 - - - - - -

Center Synechocystis - 6 - - - - - - - - - -

Center Tetraedron - - - - - 1 - - - 2 - 1

Center Tetraedron caudatum - - - - - - - 1 - 2 - -

Center Tetraedron gracile 1 - - - - - - - - - - -

Center Tetraedron minimum - - - - - 1 - - - - - -

Center Tetraedron muticum - 1 - - - - - - - - - -

Center Tetraedron trigonum

gracile

- - 2 - 2 - - - - - - -

Center Tetrastrum

heterocanthum

1 - 3 - 1 - - - - - - -

Center Treubaria

triappendiculata

- - - 1 - - - - - - - -


Page 43 of 96

Site Taxon 2016-

7-25

2016-

8-23

2016-

9-27

2016-

10-19

2016-

11-29

2016-

12-27

2017-

1-30

2017-

2-28

2017-

3-29

2017-

4-11

2017-

5-23

2017-

6-29

Center Xanthophyceae - - - 1 - - - - - - - -

Northeast Actinastrum hantzschii - - - 1 3 1 - - - - - -

Northeast Anabaena - - - - - - - - - - - 1

Northeast Ankistrodesmus falcatus 2 - 2 - 1 3 - - - - - 4

Northeast Ankistrodesmus

fusiformis

- 1 - - - 1 - - - - 1 3

Northeast Aphanizomenon - - - - - - - - - - - 1

Northeast Aphanizomenon

flosaquae

- 15 43 - 1 2 - 2 2 - - -

Northeast Aphanocapsa - - - - - 2 3 - - 24 - 7

Northeast Aphanocapsa conferta - - - 5 - - 3 - - 5 - -

Northeast Aphanocapsa

delicatissima

- 10 3 1 20 9 2 29 34 1 13 13

Northeast Aphanocapsa elachista - - - - - - - - - 1 - -

Northeast Aphanocapsa incerta - 7 10 - 7 - - 11 22 - 7 2

Northeast Aphanocapsa

planctonica

- - - 2 - 1 3 4 - 4 - -

Northeast Aphanothece - 3 1 - - 7 - 3 10 1 10 15

Northeast Aphanothece clathrata - - - 4 - 6 12 - - 35 - 12

Northeast Aphanothece smithii - - - - - 1 3 - - - - -

Northeast Bacillariophyta 132 54 50 25 108 64 37 51 35 10 67 27

Northeast Botryococcus braunii 1 - - - - - - - - - 2 -


Page 44 of 96

Site Taxon 2016-

7-25

2016-

8-23

2016-

9-27

2016-

10-19

2016-

11-29

2016-

12-27

2017-

1-30

2017-

2-28

2017-

3-29

2017-

4-11

2017-

5-23

2017-

6-29

Northeast Centritractus

belanophorus

1 - - 1 - - - - - - - -

Northeast Chlamydomonas 1 7 11 - - - - - - - 1 1

Northeast Chlorella - - 5 - - - - - - - - 5

Northeast Chlorophyceae - - - 21 - - 27 - - 25 - 9

Northeast Chodatella subsalsa - - - - - - - - - 1 - -

Northeast Chroococcales - - - 27 - - - - - - - -

Northeast Chroococcus - - - 1 - 11 - - - - - 7

Northeast Chroococcus dispersus - - - 1 3 - - - - - - -

Northeast Chroococcus minimus - - - - - - 2 - - 2 - -

Northeast Closterium 1 4 4 - - 1 - 1 - - - 1

Northeast Closterium acutum linea - - - 1 - - - - - - - -

Northeast Closterium gracile - - - - - - - 2 - - - -

Northeast Coccomonas - - 7 - - - - - - - - -

Northeast Coccomonas orbicularis 1 - - - - - - - - - - -

Northeast Coelastrum microporum - 3 - - - - - - - - - -

Northeast Coelastrum

proboscideum

- - - - 2 - - - - - - -

Northeast Coelastrum sphaericum - - - - - - 1 - - - - -

Northeast Coelosphaerium - - - 1 - - - - - - - -

Northeast Coenochloris fottii - - - 1 - - - - - 1 - -


Page 45 of 96

Site Taxon 2016-

7-25

2016-

8-23

2016-

9-27

2016-

10-19

2016-

11-29

2016-

12-27

2017-

1-30

2017-

2-28

2017-

3-29

2017-

4-11

2017-

5-23

2017-

6-29

Northeast Cosmarium - - - - - - - - - 3 - 3

Northeast Crucigenia apiculata - - - - 2 1 - - - - - -

Northeast Crucigenia irregularis - - - - 1 - - - 2 - - -

Northeast Crucigenia rectangularis - - 1 - - - - - 5 - - 1

Northeast Crucigenia tetrapedia - 7 - - - - - 3 - - - -

Northeast Cryptomonas - 1 1 - - - - - - - - 1

Northeast Cryptophyceae - - - - - - 1 - - - - -

Northeast Cuspidothrix

issatschenkoi

- 1 - - 1 - - - - - - -

Northeast Cyanodictyon - 8 2 - 5 - - 10 33 - 3 -

Northeast Cyanodictyon

imperfectum

- - - 38 - 53 111 - - 107 - 31

Northeast Cyanophyceae - - - 1 - 8 16 - - 17 - 4

Northeast Cylindrospermopsis

gangetica

- - 2 - 1 - - - - - - 3

Northeast Cylindrospermopsis

raciborskii

27 2 1 3 1 11 4 22 17 2 31 14

Northeast Dactylococcopsis

acicularis

- - - 5 - - - - - - - -

Northeast Desmatractum indutum - - - 1 - - - - - - - -

Northeast Diacanthos

belenophorus

2 - - - - - - - - - - -

Northeast Dichotomococcus - - - 1 - - - - - - - -


Page 46 of 96

Site Taxon 2016-

7-25

2016-

8-23

2016-

9-27

2016-

10-19

2016-

11-29

2016-

12-27

2017-

1-30

2017-

2-28

2017-

3-29

2017-

4-11

2017-

5-23

2017-

6-29

Northeast Dictyosphaerium - - - - - - 7 - - - - 2

Northeast Dictyosphaerium

ehrenbergianum

2 - - - 2 1 - - 8 - 3 -


pulchellum

- 15 10 1 9 6 2 11 7 - 23 2


tetrachotomum

- - - 2 - - - - - 1 - -

Northeast Dolichospermum - - - 1 - - - - - - - 1

Northeast Dolichospermum

circinale

4 28 34 - - - - 2 - - - -


perturbatum

- 1 - - - - - - - - - -


planctonicum

1 2 - - - - - - - - - -


spiroides

- 1 - - - - - - - - - -

Northeast Elakatothrix gelatinosa - 1 - - - - - - - - 1 -

Northeast Elakatothrix viridis - - - - - - - - - 1 - -

Northeast Euastrum - - - - - - - - 1 - - 2

Northeast Eudorina elegans 1 1 - - - 1 - - - - - -

Northeast Glenodinium 81 18 8 - - - - - - - - -

Northeast Gloeocapsa - - - - - - - - - - - 2

Northeast Gloeocystis - 2 - - - - - - - - - -


Page 47 of 96

Site Taxon 2016-

7-25

2016-

8-23

2016-

9-27

2016-

10-19

2016-

11-29

2016-

12-27

2017-

1-30

2017-

2-28

2017-

3-29

2017-

4-11

2017-

5-23

2017-

6-29

Northeast Goniochloris - - - 1 - - - - - - - -

Northeast Kirchneriella - 5 10 - 1 - 1 - 1 - 2 1

Northeast Kirchneriella lunaris - - - - - 1 - - - - - -

Northeast Kirchneriella lunaris

irregularis

- - - 1 - - - - - - - -

Northeast Kirchneriella obesa - 3 - - - - - - 1 - - 1

Northeast Koliella - 1 - - 7 1 - 14 - - - 1

Northeast Koliella longiseta - - - - - 2 - - - - - -

Northeast Lagerheimia - - - 1 - 1 - - - - - -

Northeast Lagerheimia

wratislaviensis

- - - 1 - - - - - - - -

Northeast Lepocinclis ovum 1 - - - - - - - - - - -

Northeast Mallomonas - - - - - 1 - - - - - -

Northeast Merismopedia punctata - - - - 2 1 - - - 1 - -

Northeast Merismopedia

tenuissima

1 1 2 3 13 1 6 2 6 11 9 -

Northeast Merismopedia

warmingiana

- 12 21 - 8 9 - 10 22 - 35 3

Northeast Micractinium - - - - - 1 - - - - - -

Northeast Micractinium pusillum - - - - - 1 - - - - - -

Northeast Microcystis - - - - - - 2 - - 1 - 15

Northeast Microcystis aeruginosa 1 8 3 - - - - 2 - - 5 -


Page 48 of 96

Site Taxon 2016-

7-25

2016-

8-23

2016-

9-27

2016-

10-19

2016-

11-29

2016-

12-27

2017-

1-30

2017-

2-28

2017-

3-29

2017-

4-11

2017-

5-23

2017-

6-29

Northeast Microcystis wesenbergii 1 - 2 - - 1 - - 5 - 3 3

Northeast Monoraphidium - 7 6 - 3 4 - 3 2 1 3 -

Northeast Monoraphidium

arcuatum

- - - 1 - - 3 - - - - 8


circinale

- - - 5 - - - - - - - -


contortum

- - - 1 - - - - - 3 - -


griffithii

- - - - - - 4 - - 2 - -


irregulare

- - - 2 - - 9 - - 2 - -


komarkovae

- - - - - - - - - - - 1

Northeast Nephrocytium - - - - - - - - - 1 - 1

Northeast Nostocales - - - 1 - - 2 - - - - -

Northeast Oedogonium - - - - - - - - - 1 - -

Northeast Oocystis - 10 1 - - - - 4 1 1 4 3

Northeast Oocystis parva - - - - - - - - - 1 - -

Northeast Ophiocytium capitatum

longispinum

- - 1 - - - - - - - - -

Northeast Oscillatoriales - - - - - - 2 - - - - -

Northeast Pediastrum - - - - - - - - - - - 1


Page 49 of 96

Site Taxon 2016-

7-25

2016-

8-23

2016-

9-27

2016-

10-19

2016-

11-29

2016-

12-27

2017-

1-30

2017-

2-28

2017-

3-29

2017-

4-11

2017-

5-23

2017-

6-29

Northeast Pediastrum boryanum - - - - - 2 - - - - - -

Northeast Pediastrum duplex 1 - - - - 1 - - - - - -

Northeast Pediastrum tetras 1 - 1 - - - - 1 - - - -

Northeast Peridinium - 4 2 - - - - - - - - -

Northeast Phacus - 6 1 - - - - - - - - -

Northeast Planctonema

lauterbornii

- - - 1 - - - - - - - -

Northeast Planktolyngbya contorta 2 9 6 12 9 14 17 12 29 12 30 17

Northeast Planktolyngbya

limnetica

1 2 2 2 29 22 8 29 12 14 18 11

Northeast Planktothrix agardhii 3 - - - - - - - - - - -

Northeast Pseudanabaena 3 - 6 - 5 5 4 - - - - -

Northeast Pseudanabaena

mucicola

- - - - - - - - - - - 10

Northeast Pseudodidymocystis

fina

- - - - - - 2 - - - - -

Northeast Quadrigula chodati - 3 - - 3 - - 5 1 - 1 -

Northeast Quadrigula closteroides - - - - - - 1 - - - - -

Northeast Radiocystis geminata - - - - 7 - - - - - - -

Northeast Rhabdoderma - 2 - - - - - - - - - -

Northeast Rhabdogloea 4 4 1 - 5 8 - 11 1 - 2 7

Northeast Romeria - 2 3 - 2 2 - 3 - - 1 2


Page 50 of 96

Site Taxon 2016-

7-25

2016-

8-23

2016-

9-27

2016-

10-19

2016-

11-29

2016-

12-27

2017-

1-30

2017-

2-28

2017-

3-29

2017-

4-11

2017-

5-23

2017-

6-29

Northeast Romeria leopoliensis - - - 1 - - 2 - - - - -

Northeast Scenedesmus - - - 6 - 5 14 - - 7 - 14

Northeast Scenedesmus abundans - 6 3 1 7 2 7 2 2 1 3 4

Northeast Scenedesmus

acuminatus

2 1 - 1 - 1 - 1 1 - - -


acutiformis

- 2 - - - - - 6 7 - 1 -

Northeast Scenedesmus bicaudatus 3 - 1 - - 2 - 4 2 - 1 1

Northeast Scenedesmus bijuga 1 2 7 - 2 1 - 3 - - - 1


brasiliensis

1 - - - - 1 - - - - - -

Northeast Scenedesmus dimorphus - - - - - 2 - 1 - - - -

Northeast Scenedesmus obtusus - - - 1 - - - - - - - -

Northeast Scenedesmus opoliensis - - - - 2 - - - - - - -

Northeast Scenedesmus perforatus 3 - 1 1 5 - 4 - 3 - 1 -


quadricauda

9 2 4 - 12 9 - 15 13 - 5 5

Northeast Scenedesmus verrucosus - - - - - - - - 1 - 2 -

Northeast Schroederia judayi 1 6 4 - 4 - - - - - - -

Northeast Schroederia setigera - - - 1 1 - - - - - - -

Northeast Selenastrum - - - - - 1 - - - - 1 -

Northeast Selenastrum westii 1 - - - - - - - - - - -


Page 51 of 96

Site Taxon 2016-

7-25

2016-

8-23

2016-

9-27

2016-

10-19

2016-

11-29

2016-

12-27

2017-

1-30

2017-

2-28

2017-

3-29

2017-

4-11

2017-

5-23

2017-

6-29

Northeast Snowella - 9 14 - 6 - - 16 15 - 17 8

Northeast Snowella

Ecological Assessment of Lake Istokpoga · 2019. 5. 23. · Ecological Assessment of Lake Istokpoga – May 2019 Page 5 of 96 mean chlorophyll a value of less than 20 μg/L. Annual

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