Easy microbial monitoring of entire chicken flocks

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Easy microbial

monitoring of entire

chicken flocks

November 2019 | Dr. Anita Menconi

Evonik at Poultry Tech Summit 2020

Atlanta, GA

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EvonikWhat we don't do

NUTRITIONTOWELS TABLETSCAR TIRES MATTRESSES

What we doLiving better with Evonik

more effect iveheal th ierf lu ff iermore fue l saving more e las t ic

Countless products gain their special attributes through our creative power.

| November 2019 | Easy microbial monitoring of entire chicken flocks | Poultry Tech Summit | Dr. Anita Menconi

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Our structureStrategic concentration and operative independence

Performance Materials

Polymer materials and intermediates mainly for the rubber, plastics and agriculture industries

Nutrition & Care

Products for use in the areas consumer goods, nutrition and health

Resource Efficiency

Environmentally friendly and energy-efficient systems as solutions for several industries

Technology & Infrastructure

Site operation, energy & utilities, technical services, logistics, process technology and engineering

Segments

Strategic Management Holding


4

Evonik Animal NutritionThe core: Amino acids and Gut Health Solutions


Services▪ MetAMINO®

▪ Mepron®

(rumen

protected

methionine)

▪ AQUAVI®

Met-Met

▪ Biolys®

▪ ThreAMINO®

▪ TrypAMINO®

▪ ValAMINO®

Amino

acids

▪ Ecobiol®

▪ Fecinor®

▪ GutCare®

▪ GutPlus®

▪ AMINOLab®

▪ AMINODat®

▪ AMINONIR®

Advanced

▪ AMINONRG®

▪ AMINOProx®

▪ AMINORED®

▪ AMINOInsight®

▪ AMINOBatch®

▪ AMINOBatch®

WPT

▪ AMINOSys®

▪ AMINOCHICK®,

AMINOHEN®

▪ AMINOPig®

▪ AMINOCow®

▪ AMINOShrimp®,

AMINOTilapia®

▪ AMINOFootprint®

▪ Animal nutrition &

Gut health

consulting

The shell: Software and services combined

Amino

acids

The completion: nutrition and health concepts

Standardized

ileal digestibility

(SID)

Net

energy

systems

Low

protein

diet

Nutritional

recommen-

dations

Amino

acids

n

The holistic approach: precision livestock farming (PLF)

Amino

acids

▪ FloXControl®

▪ ScreenFloX®

▪ Stratify individual farm animals or

flocks to enable optimized

management and feeding

▪ Improve, track, and trace animal

welfare and sustainability

▪ Benefit from artificial intelligence,

cloud solutions, and Internet-of-

Things

5 | November 2019 | Easy microbial monitoring of entire chicken flocks | Poultry Tech Summit | Dr. Anita Menconi

A new way of thinking flock health

▪ Current approach: Individual animal, infectious diseases (clinical), uses invasive methods

▪ Health focus to sustain a livestock

▪ Evonik screening approach: entire flock, use of non-invasive methods to assess the status of a livestock herd/flock

▪ Fast turnaround time due to on-site

▪ The only system solution (reliable, robust) that can be expanded to other parameters and enables customer to optimize

their production process.

Common

practice

New

approach

Probiotics, ….

By veterinarian:

e.g. therapeutical

antibiotics

Intervention by medication

Prevention / soft intervention

6 | November 2019 | Easy microbial monitoring of entire chicken flocks | Poultry Tech Summit | Dr. Anita Menconi

How do we achieve this?From single bird diagnostic towards a powerful herd approach

Pick a few →

Diagnose each individuum

Collect feces randomly, representing the flock by

pooling into one sample

Diagnostic

Test/Device

Environmental

Screening Test

~ 6 x

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Evonik microbial screening PCR platform “ScreenFloX®”: addressing various customer needs via several tests

*PCR = polymerase chain reaction (genome cloning and sequencing tool)

**One-for-all workflow (identical settings)

Human

pathogenic

Avian

pathogenic

Control of

livestock

production

process

On-farm workflow** (6 hours to overnight)

Result/InterpretationAnalysisPre-treatment Sample Prep.Sample Collection React

• Machine settings

identical for all tests

• All tests use the same

collection & sample

preparation chemistry


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Sample collection and laboratory assay – Workflow

Heating Grinding Spinning Extraction qPCR

Sample collection @ study site

Perform assay @ lab site → 3 hours

- 5-20 g of fecal material / per tube

- Storage at RT/-20°C up to 7 days

- Extraction and qPCR device fixed

- SOP – workflow


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Utilizing PCR as a screening solution requires standardization


Customer Requirements:

A screening device must be:

• Reliable

• Reproducible

• Accurate & precise

• Specific & sensitive

• (Quantitative)

Evonik‘s Approach:

Reduction of variances:

• Control the sample

• Standardize workflow

• Use of premium quality

chemistry

• Superior production

process (ISO 13485)

Reduction of errors:

• Automated workflow

• Use of internal run control

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Functional design of the ScreenFloX® PCR C. perfringens DNA test (quant.) –The Kit allows easy monitoring of health and microbial status of entire flock

Detection React

Recommendation

• Soft intervention

• Treatment decision

Status of the flock

B A

B A

Ratio 0,8

Ratio 0,1

Interpretation

‚Healthy‘

’Affected’

Act

Caution

OK

Act

Caution

OK

A B

A B


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Testing Kit performance in the field - ScreenFloX® for Clostridium perfringens

NE outbreak

(pathology confirmed)

netB cpa

control

(no pathology)

Day

17

For each flock 96 randomly collected samples were pooled into one sample that was further analyzed

4 flocks each tested

• All samples contained Clostridium perfringens - tested positive through cpa

• All NE-positive flocks were tested netB positive

• All NE-negative flocks were tested netB negative


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ScreenFloX® at a Glance


Performance

Trust

Openness

Speed

• Quantitative real-time PCR tests with

high linear range

• Highly sensitive, specific, accurate and

precise

• Robust, reliable

• Validated system solution to minimize

variances (workflow, internal control,

etc.) → optimized workflow

• Representative (whole flock)

• Sample to result: 3 hrs*

* Depending on numbers of samples processed and the customer set-up

• Scalable: up to 80 samples per shift

• Expandable: Up to 5 different tests at

any time on the instrument. Unlimited

test menu (viruses, bacteria, protozoa)

• Compatible: Modular nature makes

system compatible to other workflows

ScreenFloX® is a molecular screening solution to monitor the microbial burden of herds in order to improve the

production process and de-risk livestock production

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Easy microbial monitoring of entire chicken flocks

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