E. Coli What's in your water?. Have you ever wondered what is in your water?

E. Coli What's in your water?

Have you ever wondered what is in your water?

Is Spring or Stream Water more Contaminated

with E. Coli?

Research• According to the USDA and the EPA,

agricultural run-off can greatly contaminate water sources• E. coli from animal dung

Springs vs. Streams• Springs are underground streams.• Springs are naturally filtered.• Streams are shallow rivers.• Streams are exposed to contaminants

Hypothesis

Stream water will have the higher E. coli levels than spring water.

Experimental Procedure- prepare the sample

1. Collect water samples in sterilized 25 mL glass jars2. Add 5 mL of sampled water to bottle of nutrients and shake for 5 seconds

Materials

For this experiment you would need.• Incubator• Petri dishes and corresponding supplies• Eye droppers• Water samples• Rubber gloves• Counting

Experimental Procedure- growing the bacteria

3. Place the sample in a Petri dish after 3 minutes the solution gels. Invert dishes5. The Petri dishes were incubated for 3 days at 23-26 C4. Colonies are counted and results are graphed 5 Repeat steps 2 through 4 for all samples

Incubation• Samples are incubated• Maintain a constant temperature of

twenty-three(23) to twenty-six(26) degrees Celsius

• All samples were isolates to a sterile surface to control cross contamination

Experimental Procedure Counting• Count E.coli in each Petri dish

-use a grid over the Petri dish and count the colonies

- to ensure accurate data repeat counts

Control Group

• Distilled water- most impurities removed

–Zero E. Coli should be present

CONTROLColiform Growth Count per 5mL sample.

Distilled Water 1 0Distilled Water 2 0Distilled Water 3 0Distilled Water 4 0Distilled Water 5 0

STREAMSColiform Growth Count per 5mL sample.

North Side Stream 55 53

Little Juniata 18 18

Sugar Run 85 84

Riggles Gap Run 42 43

Piney Creek (stream) 48 50

SPRINGSColiform Growth Count per 5mL sample.

Roaring Spring 0 0Piney Creek Spring 10 13Sparkling Spring 44 43Woodland Spring 11 10Locke Mt. Spring 13 12

Distille

d 1

Distille

d 2

Distille

d 3

Distille

d 4

Distille

d 5

Northsid

e Stre

am

Little

Juniata

Suga

r Run

Riggles

Gap Run

Piney Cree

k

Roaring S

pring

Piney Cree

k Sprin

g

Spark

ling S

pring

Woodlan

d Sprin

g

Locke

Mt. S

pring

0

10

20

30

40

50

60

70

80

90

0 0 0 0 0

55

18

85

42

48

0

10

44

1113

Average Coliform Growth Count per 5mL sample

Sample Source

Colo

ny C

ount

Roaring Spring Piney Creek Spring Sparkling Spring Woodland Spring Locke Mt. Spring0

5

10

15

20

25

30

35

40

45

50

0

10

44

1113

Average Coliform Growth Count per 5mL sample of Spring Water

Sample Source

Colo

ny C

ount

Northside Stream Little Juniata Sugar Run Riggles Gap Run Piney Creek0

10

20

30

40

50

60

70

80

90

55

18

85

42

48

Average Coliform Growth Count per 5mL Sample of Stream Water

Sample Source

Colo

ny C

ount

Average Coliform Count in 5mL sample

Distilled 0

Stream 49.6

Spring 15.6

ConclusionThe data does not support my hypothesis that stream water has more E. coli than spring water. Due to the individuality of streams and springs.

Further Improvements

to test streams and their corresponding feeder springs to determine the contamination levels from the source and down-stream. the source of the contamination along the waterway.

Abstract

• Is Spring or Stream Water more Contaminatedwith E. Coli?

• Stream water will have the higher E. coli levels than spring water.

• The data supports my hypothesis that stream water has more E. coli than spring water.