豊 トヨノ 野 孝 タカシ 略 歴 1992年 九州大学農学部農芸化学科 卒業 1994年 九州大学大学院 遺伝子資源工学専攻修士課程 修了 1997年 九州大学大学院 遺伝子資源工学専攻 博士後期課程 修了 博士(農学) 取得 1997-1998 年 九州大学生体防御医学研究所 非常勤研究員 1998-2007 年 九州歯科大学 口腔組織機能解析学分野 助手 2007年-現在 九州歯科大学 口腔組織機能解析学分野 助教 共同研究者 豊島 邦昭 (九州歯科大学 口腔組織機能解析学分野 教授) 瀬田 祐司 (九州歯科大学 口腔組織機能解析学分野 准教授) 片岡 真司 (九州歯科大学 頭頸部構造解析学分野 助教) 味蕾の味覚情報伝達系におけるアディポネクチンの機能解明 Expression of adiponectin receptors in mouse taste buds. Adiponectin is a white adipose protein that plays important roles in glucose homeostasis and lipid metabolism and is involved in cell proliferation and differentiation. The function of adiponectin is mediated by its receptors (AdipoR1 and AdipoR2), which have different expression patterns. In the gustatory system, adiponectin is contained in the saliva; however, the function of adiponectin signaling in gustatory tissues has not been elucidated yet. Therefore, we have examined the expression patterns of AdipoR1 and AdipoR2 in mouse gustatory tissues. Reverse transcription polymerase chain reaction assays have revealed that AdipoR1 and AdipoR2 mRNAs were expressed in the circumvallate papillae. Using immunohistochemistry, the anti-AdipoR1 antibody labeled at the taste hairs in the fungiform, foliate and circumvallate papillae. Double-labeling experiments have demonstrated an expression pattern of AdipoR1-positive cells in a subset of basal cells within the taste buds and extragemmal epithelium cells to marker proteins for taste cell types (including typesⅠ,Ⅱ,Ⅲ, and basal cells). For the marker proteins analyzed, only Shh was co-expressed with AdipoR1 in a subset of basal cells within the taste buds in the circumvallate papillae. These results show that AdipoR1 at taste hairs in gustatory tissues may detect saliva adiponectin and may play a role in taste sensing in the taste buds. In addition, AdipoR1 may play a role in cell proliferation and differentiation for the Shh and AdipoR1 expressing basal cells within the taste buds. 1
This document is posted to help you gain knowledge. Please leave a comment to let me know what you think about it! Share it to your friends and learn new things together.
Expression of adiponectin receptors in mouse taste buds.
Adiponectin is a white adipose protein that plays important roles in glucose homeostasis and lipid metabolism and is involved in cell proliferation and differentiation. The function of adiponectin is mediated by its receptors (AdipoR1 and AdipoR2), which have different expression patterns. In the gustatory system, adiponectin is contained in the saliva; however, the function of adiponectin signaling in gustatory tissues has not been elucidated yet. Therefore, we have examined the expression patterns of AdipoR1 and AdipoR2 in mouse gustatory tissues. Reverse transcription polymerase chain reaction assays have revealed that AdipoR1 and AdipoR2 mRNAs were expressed in the circumvallate papillae. Using immunohistochemistry, the anti-AdipoR1 antibody labeled at the taste hairs in the fungiform, foliate and circumvallate papillae. Double-labeling experiments have demonstrated an expression pattern of AdipoR1-positive cells in a subset of basal cells within the taste buds and extragemmal epithelium cells to marker proteins for taste cell types (including typesⅠ,Ⅱ,Ⅲ, and basal cells). For the marker proteins analyzed, only Shh was co-expressed with AdipoR1 in a subset of basal cells within the taste buds in the circumvallate papillae. These results show that AdipoR1 at taste hairs in gustatory tissues may detect saliva adiponectin and may play a role in taste sensing in the taste buds. In addition, AdipoR1 may play a role in cell proliferation and differentiation for the Shh and AdipoR1 expressing basal cells within the taste buds.
1) Kawai K, Sugimoto K, Nakashima K, Miura H and Ninomiya Y: Leptin as a modulator of sweet taste sensitivities in mice. Proc. Natl. Acad. Sci. U S A 97, 11044-11049 (2000).
2) Kadowaki T, Yamauchi T and Kubota N: The physiological and pathophysiological role of adiponectin and adiponectin receptors in the peripheral tissues and CNS. FEBS Lett. 582, 74-80 (2008).
6
3) Kubota N, Yano W, Kubota T, Yamauchi T, Itoh S, Kumagai H, Kozono H, Takamoto I, Okamoto S, Shiuchi T, Suzuki R, Satoh H, Tsuchida A, Moroi M, Sugi K, Noda T, Ebinuma H, Ueta Y, Kondo T, Araki E, Ezaki O, Nagai R, Tobe K, Terauchi Y, Ueki K, Minokoshi Y and Kadowaki T: Adiponectin stimulates AMP-activated protein kinase in the hypothalamus and increases food intake. Cell metabolism 6, 55-68 (2007).
4) Toda M and Morimoto K: Comparison of saliva sampling methods for measurement of salivary adiponectin levels. Scand J Clin Lab Invest 68, 823-825 (2008).
5) Miura H, Kusakabe Y and Harada S: Cell lineage and differentiation in taste buds. Arch histol cytol 69, 209-225 (2006).
6) Kawai K, Kageyama A, Tsumano T, Nishimoto S, Fukuda K, Yokoyama S, Oguma T, Fujita K, Yoshimoto S, Yanai A and Kakibuchi M: Effects of adiponectin on growth and differentiation of human keratinocytes-implication of impaired wound healing in diabetes. Biochem Biophys Res Commun 374, 269-273 (2008).
7) Chen K, Yan J, Suo Y, Li J, Wang Q and Lv B: Nutritional status alters saccharin intake and sweet receptor mRNA expression in rat taste buds. Brain Res 1325, 53-62 (2010).