DWFP Toxicology Research to Develop Insecticides for Vector Control Julia W. Pridgeon, PhD Toxicologist/Molecular Biologist 02-14-07 Mosquito and Fly Research Unit Center for Medical, Agricultural, and Veterinary Entomology USDA-ARS, 1600 SW 23rd Drive Gainesville, FL 32608
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DWFP Toxicology Research to Develop Insecticides for Vector Control Julia W. Pridgeon, PhD Toxicologist/Molecular Biologist 02-14-07 Mosquito and Fly Research.
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DWFP Toxicology Research to Develop Insecticides for Vector Control
Julia W. Pridgeon, PhDToxicologist/Molecular Biologist
02-14-07
Mosquito and Fly Research UnitCenter for Medical, Agricultural, and
Veterinary EntomologyUSDA-ARS, 1600 SW 23rd Drive
Gainesville, FL 32608
Research Objective
To discover and develop effective
chemical and molecular
insecticides for vector control
Military Relevance
♣ In military units, arthropod-borne diseases continue to be serious threats to deployed troops
♣ Chemical pesticides are the most effective way to control disease vectors, however, few products are available for military use
♣ Due to the nature of military operations, pesticides that can quickly and effectively eliminate arthropod vectors are highly desirable
Approach for the Development of Effective Chemical Insecticides
Registered Pesticides
Natural PlantChemicals
Plant ExtractsDesigner
Chemicals
USDA-ARS-NPURU
USDA-ARS-MFRU
NPURU & UF IRAC
High-Throughput Screen to find highly toxic chemical(Topical application; Aedes aegypti model)
Small Chamber Test and Field Evaluation
Topical Application
EasyAccurate
Small Amount Fast
Results of Studies on Chemical Insecticides
Registered Pesticides
Natural PlantChemicals
Plant Extracts
33
DesignerChemicals
USDA-ARS-NPURU
USDA-ARS-MFRU NPURU & UF IRAC
Pesticide LD50 (ug)
Fipronil 1.3 10-6
Permethrin 1.4 10-4
Spinosad 2.5 10-3
Bifenzate 4.25
19 26
AT-05-27-2(LD50 0.3ug)
123-1(LD50 = 0.4ug)
Bacopa monnieri (Water hyssop)Methanol Extract
LD50 125ug
33
LD50 =0.8ug
LD50 =2.1ug
LD50 =14.72ug
Summary on Studies of Chemical Insecticides
1)Screened 19 registered pesticides; several showed high insecticidal activity against Aedes aegypti
2)Screened 26 different natural plant chemicals (NPURU); two had relatively high toxicities
3) Screened 33 different plant extracts (MFRU), some had higher toxicity against mosquitoes than others
4) Screened 33 piperidines with different structures (UF and NPURU), 2-ethyl piperidines showed the highest toxicities against mosquito (in press in Journal of Medical Entomology)
Summary on Studies of Chemical Insecticides
Future Studies on Chemical Pesticides
1)Evaluate the toxicities of registered pesticides against different species of mosquitoes and other disease vectors
2)Select the best pesticide candidates for further evaluation in small chamber tests and field studies
3)Manipulate the structures of plant chemicals and evaluate their toxicities
4) Studies on toxicity of plant extracts will be continued (additional information by Dr. Quinn)
5) For structure-activity analysis, different classes of chemicals will be evaluated and the best ones chosen for further structure manipulation
Future Studies on Chemical Pesticides
Approach for the Developmentof Molecular Pesticides