Stimulation of barrier functions by digitoxin in NCI-H460 cells Hosam A. Elbaz 1 , Reem El Dawud 2 , Todd A. Stueckle 1,3 , Liying Wang 3 , Yon Rojanasakul 1 , and Cerasela Zoica Dinu 2 1 Department of Basic Pharmaceutical Sciences, West Virginia University, 2 Department of Chemical Engineering, West Virginia University, 3 National Institute for Occupational Safety and Health Funding: WVNano, NSF/SEED (EPS-1003907 ) WVU School of Pharmacy
This document is posted to help you gain knowledge. Please leave a comment to let me know what you think about it! Share it to your friends and learn new things together.
Transcript
Stimulation of barrier functions by digitoxin in NCI-H460 cells
Hosam A. Elbaz1, Reem El Dawud2, Todd A. Stueckle1,3, Liying Wang3, Yon Rojanasakul1, and Cerasela Zoica Dinu2
1Department of Basic Pharmaceutical Sciences, West Virginia University, 2Department of Chemical Engineering, West Virginia University,
3National Institute for Occupational Safety and Health
Funding: WVNano, NSF/SEED (EPS-1003907 )WVU School of Pharmacy
Introduction
Wang et al., 2010
Cell cycle arrest
Elbaz et al., in press
Objective and Hypothesis• Objective:
– To study the cytotoxic effects of digitoxin in real-time using electrical impedance technology.
• Hypothesis:
– Digitoxin will induce morphological changes in NCI-H460 cell monolayer over time.
– Morphology changes correlates with digitoxin’s cytotoxic effect on NCI-H460 cells.
Electric cell-substrate impedance sensing (ECIS)
http://www.biophysics.com/ecis-theory.php
Z= (R2 + Xc2) 0.5
Zmodel (f) = f {Cm, α, Rb}
• Rb : barrier function of the cell layer
• α : current flow beneath the cell
• Cm : cell membrane capacitance
[Giaever, I. and Keese, C.R., PNAS 81, 3761 (1991)]
Digitoxin at 20 nM increases the resistance of the cell monolayer formed by NCI-H460 cells
Control
20 nM
40 nM
80 nM
Au electrode
Several possibilities can explain the increased resistance due to 20 nM digitoxin