About Lemna. • Duckweed (Lemnaceae) is a small floating macrophyte • 4 genus (Lemna, Landoltia, Spirodela and Wolffia) and 40 known species • Duckweed naturally grows in river banks or lakes but it is also been used for wastewater phytoremediation through lagoons or raceway systems • Capacity to duplicate weight in 16 h-2 days • High productivity per surface (see table). • High absortion capacity N and P: 2 and 0.5 ton·ha -1 ·year -1 • Biomass composition rich in proteins 22.5-29.3 %, carbohydrates 10-32.5 % and lipids 8.7-11.2 %. Suitable for many biobased products Duckweed technology for improving nutrient management and resource efficiency in pig production systems Life LEMNA aims to reduce manure nutrient pollution of water bodies in farming areas, improve resource efficiency and close the mineral cycle. A 250 m2 duckweed-based cultivation system will be designed and built to daily treat about 3.000L of digestate and produce >35 kg of duckweed biomass. A biogas plant located in a pig farm will be the project site for demo trials. The duckweed biomass will be used as a feedstock for producing a biofertiliser and feed which will be evaluated. Objectives and scope: Appenroth K.A., Sree K. S., Böhm V., Hammann S., Vetter W., Leiterer M., Jahreis G. (2017). Nutritional value of duckweeds (Lemnaceae) as human food. Food Chemistry. 217 pp. 266-273. Mohedano R. A., Costa R. H. R., Tavares F. A., Belli P. (2012). High nutrient removal rate from swine wastes and protein biomass production by full-scale duckweed ponds. Bioresource Technology. 112, pp. 98-104. Xu J., Shen G. (2011a). Growing duckweed in swine wastewater for nutrient recovery and biomass production. Bioresource Technology. 102, pp. 848-853. www.life-lemna.eu/ Farm Biogas plant Duckweed production Biofertilizer production Crop farming Animal feed production LIFE 2015 Agreement Number LIFE15 ENV/ES/000382 Lemna project is being developed under the support of the Life Programme of the European Commission Duckweed for nutrient recovery and/or biomass production Potential applications of duckweed biomass Foreseen results Pioneer prototype in the EU for duckweed cultivation on digestate. A collection of 25 duckweed strains will be developed. 95-100% nutrient (N and P) recovery efficiency rates treating anaerobically digested pig manure Validation of new duckweed biobased products: feed /biofertiliser Carbon fooprint reduction of animal production. e-LEMNATOOL for system replication in other EU farms. ainia´s technical resources and know-how Manure Thermal use of biogas Digestate liquid fraction Digestate solid fraction Biofertilizers soil amendments Animal feed Vegetable raw materials Food, pharma, cosmetic Ingredients and additives to innovative products Animal feed High quality protein for fish and other farm animals Biobased chemicals Cheap and sustainable sugars for bioprocesses Biofuels Biocarbon, bioetanol, biogas Biofertilisers Aminoacids and other bioestimulants to liquid biofertilisers Biomaterials Bioplastics, biocomposites and other biomaterials • Laboratory (1 L) and pilot scale duckweed (50-200L) cultivation systems • Know-how on duckweed cultivation including initiation, cultivation and harvesting • Process control by light, pH, COD, morphology and other parameters Experience Duckweed morphology study Lab scale optimization cultivation 1 L biorreactor for lab scale cultivation 50 L outdoor cultivation 200 L raceway cultivation Beneficiaries AINIA, CNB-CSIC, Ecobiogas Duration 3 years, 2016-2019 Species Production MT DM/ha/year Protein % DM Protein yield MT/ha/year Duckweed 12-16 16-45 1.9-7.2 Water hyacinth 24-32 12-35 2.9-8 Homwort 10 20 2 Cattail 32 10-14 3.2-4.5 Algae 8 50 4 Small Scale Biorefining, 2016. Wageningen University & Research