Connecting Hearts and Minds DNA and Cell Reprogramming via Epigenetic Information Visual Institute of Developmental Science DNA and Cell Reprogramming via Epigenetic Information Delivered by Magnetic Fields, Sound Vibrations, and Coherent Water Carlo Ventura, MD, Ph.D. Full Professor of Molecular Biology University of Bologna, Italy VID (Visual Institute of Developmental Sciences) Laboratory of Molecular Biology and Stem Cell Engineering, NIBB , Bologna, Italy Experimental Cardiology at the Cardiovascular Department, University of Bologna, Italy
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Magnetic fields in the Wi-Fi band of 2.4 GHz, the same used with wireless Internet connections worldwide, were used with an innovative device, called the Radio Electric Asymmetric Conveyer (REAC), to modulate the ability of stem cells to differentiate or even make adult non-stem cells behave like stem cells.
The REAC technology is based upon the interaction of two oscillating magnetic fields. One is generated by cells or the entire organism, and the other is a weaker electromagnetic field produced by the REAC system. A resultant electromagnetic field induces the cells to respond with endogenous self-generated micro-currents, likely ensuing from cellular ionic fluxes. These currents are detected and conveyed back to the cells with a conveyer probe.
Magnetic Energy: A Tool for Modulation of Cell Signaling and Fate
REAC - Modulated Magnetic FieldsDrs. Salvatore Rinaldi and Vania Fontani
Radiofrequency generators and irradiating antennas (1) create a 2.4 GHz radiofrequency field; electrodes (2) connected through a cable (CV). The radio frequency field induces radiofrequency currents in the body, which are detected and conveyed back by the electrodes.
The REAC apparatus adapted to an embryonic stem cell culture system within a CO2 incubator.
Distance between the RF emitter and the culture medium is approximately 35 cm.Electromagnetic quantities (detected by receiving antenna and spectrum analyser; single radiofrequency bursts of 250 ms. with an off interval of 2.5 seconds):
• Radiated power: 2 mW,• Electric field: 0.4 V/m,• Magnetic field: 1mA/m,• Specific Absorption Rate – SAR: 0.128W/g,• Density of radio electric current flowing in the culture medium during the REAC
single radiofrequency burst (=1 A/V.m and =1000Kg/m3), J=30A/cm2.• Specific power: around 400 μW/m²
Radiofrequency Energy Loop Primes Cardiac, Neuronal, and Skeletal Muscle Differentiation in Mouse Embryonic Stem Cells:A New Tool for Improving Tissue Regeneration
Embryonic stem cell exposure to modulated magnetic fields affects the expression of stem cell related proteins and optimizes expression of pluripotent cells.
Modulated magnetic fields enhances the yield of embryonic stem cell derived cardio-myocytes and affects the quality of beating and contractile dynamics.
The 2012 Nobel Prize in Physiology or Medicine: for the discovery that mature cells can be reprogrammed to become pluripotent.
The New York TimesBy Nicolas WadeTwo scientists who were awarded the Nobel Prize in Physiology or Medicine on Monday helped lay the foundation for regenerative medicine, the hotly pursued though still distant idea of rebuilding the body with tissues generated from its own cells. They are John B. Gurdon of the University of Cambridge in England and Shinya Yamanaka of Kyoto University in Japan.Both men had to overcome false starts in life. Dr. Gurdon was told as a boy that he was wholly unsuited for biology, and Dr. Yamanaka trained as a surgeon only to find he was not so good at it.
Crucial Experimental Details:•Filtration: 450/100 nm for bacterial DNA, 450/20 nm for viral DNA.•High dilutions in water.•Mechanical agitation (vortex) between each dilution.•Excitation by the electromagnetic background of extremely low frequency, starting at 7 Hz. •The excitation is not induced when the system is shielded by a mu-metal cage.
Typical signals from aqueous dilutions of M. Pyrum. Positive signals from D-7 to D-12 dilutions.
Device for the capture and analysis of EM signals1.Coil made up of copper wire, impedance 300 Ohms.2.Plastic tube containing 1ml of the solution to be analyzed. 3.Amplifier. The solution contained viral or bacterial DNA Fragments.
Information is thought to be the essence of life, as in the DNA code. We have growing needs of separating the “informational content” of life
from its “material substrate.”
In both the philosophical and visual sense, ‘seeing is believing’ does not apply to nano-technology, for there is nothing even remotely visible. On the atomic and molecular scale, data is recorded by sensing and probing in a very abstract manner, which requires complex and approximate interpretations. More than in any other science, visualization and creation of a narrative becomes necessary to describe what is sensed, not seen.
NIBB - VID Laboratory of Molecular Biology and Stem Cell Engineering Bologna, ItalyCarlo Ventura Silvia Cantoni Francesca Bianchi Claudia CavalliniMargherita Galletti Riccardo TassinariElena OliviIrene Frascari
Rinaldi-Fontani Institute Florence, ItalySalvatore RinaldiVania FontaniAlessandro CastagnaI
Department of Biomedical Sciences, University of Sassari, ItalyMargherita MaioliSara SantanielloGianfranco PigliaruSara Gualini
NFN, ItalyEmilio Del GiudiceAlberto TedeschiGiuseppe Vitiello
Department of Chemistry and BiochemistryUCLA, LA, USAJames K. GimzewskiSarah Cross
Institute of HeartMathBoulder Creek, CA, USARollin McCraty
Prince Sultan Cardiac CenterHofuf/AlahsaKingdom of Saudi ArabiaAbdullah Abdulrhman Alabdulgader
Cell Transplant CenterDiabetes Research Institute (DRI) WorldWide DRI FederationMiller School of MedicineUniversity of Miami, USACamillo RicordiCarlo Tremolada