Doug Kremer – President Mike Kelly – Director Turf Operations
“Maximizing Earth’s Potential”
Company Background
• Founded in 1998 to develop microbiological products - Integrated Fertility Management™ (IFM) • Convert atmospheric nitrogen to plant usable forms • Stimulates root growth and increases yields
• Released first “defined” microbial products in 2000 • Azospirillum based – Agriculture and Turf • Rhizobium based - Agriculture
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The Rhizosphere
• Classified as the zone of soil under the influence of plant roots
• Due to low available carbon, the natural state of microbes in soil is starvation. In the rhizosphere, plants give off a variety of organic substances that facilitate microbial growth
• In return, the microbes provide a number of services for the plant: – Production of plant growth hormones – Solubilization of inorganic minerals – Protection from plant pathogens
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Effects of the microbial community on plant growth Beneficial Harmful
Soil Stabilization Disease Water Uptake Nutrient Competition
Growth Promotion Microbial Competition
Nitrogen Fixation
Biocontrol
Nutrient Availability Adapted from Maier 2004
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Type of fixation N2 fixed (106 metric tons per year)
Non-biological
Industrial (Haber – Bosch Process) about 50
Combustion about 20
Lightning about 10
Total about 80
Biological
Agricultural land about 90
Forest and non-agricultural land about 50
Sea about 35
Total about 175
Data from various sources, compiled by DF Bezdicek & AC Kennedy, in Microorganisms in Action (eds. JM Lynch & JE Hobbie). Blackwell Scientific Publications 1998.
Sources of Nitrogen
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Microorganisms have a central role in almost all aspects of nitrogen availability and thus for life
support on earth:
• some bacteria can convert N2 into ammonia by the process termed nitrogen fixation; these bacteria are either free-living or form symbiotic associations with plants or other organisms (e.g. termites, protozoa) – other bacteria bring about transformations of ammonia to
nitrate, and of nitrate to N2 or other nitrogen gases
• many bacteria and fungi degrade organic matter, releasing fixed nitrogen for reuse by other organisms.
• All these processes contribute to the nitrogen cycle.
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Biological Nitrogen Fixation (BNF)
BNF occurs when atmospheric nitrogen is converted to ammonia by a pair of bacterial enzymes called nitrogenase. The simplified formula for BNF is:
Air (78% N2, 20% O2) + Protons + electrons + Energy gives Ammonia
N2 (from air) + 8H+ + 8e− + 16 ATP → 2NH3 + H2 + 16ADP + 16 P
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Examples of nitrogen-fixing bacteria
– Free Living • Azotobacter
– Associated • Azospirillum
– Symbiotic • Bradyrhizobium
– Endosymbiotic • Herbaspirillum • Acetobacter • Burkholderi
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RH = Root Hair, RS = Root Surface, B = Unattached Azospirillum Bacteria B
Associative Interaction
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Azospirillum
• Fixes Nitrogen for use by cereal crops, grasses and tuber plants
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Azospirillum
• Bacteria
• Found on many plant species
• Close to the roots (Rhizoplane) and attached to the roots
• Not a nodulater
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History • The first species of the genus, originally named
Spirillum lipoferum, was isolated from soil in the Netherlands in 1925. Forgotten for half a century, Azospirillum was 'rediscovered' in the 1970s during a search for associative nitrogen fixers in the rhizosphere of Digitaria and Zea mays in Brazil. Since then, isolation of azospirilla from roots of numerous wild and cultivated plants and from different soil types has been reported from all over the world.
“Maximizing Earth’s Potential”
Azospirillum Colonization
• The first step (the adsorption step), consists of a rapid, loose, and reversible binding of Azospirillum to the root.
• The second step (the anchoring phase), the bacteria become irreversibly bound to the root surface.
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Azospirillum and root interaction, E = Zone of elongation, RH = Root Hairs
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Bacterial Attachment
B
B
RS = Root Surface
B = Attached Azospirillum Bacteria
“Maximizing Earth’s Potential”
Bacterial Attachment
B
RH = Root Hair, RS = Root Surface, B = Attached Azospirillum Bacteria
B B
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Azospirillum Case Studies
• TAZO®
– University Nebraska Lincoln (Data slides to come)
– University Wisconsin Madison – Iowa State University