Dna replication

DNA REPLICATION

5 ENZYME &

1 PROTEIN

The Process involve :

POLYMERASE

DNA POLYMERASE III DNA POLYMERASE I

SINGLE STRANDED BINDING PROTEIN HELICASE

LIGASE

unwind or ‘unzip’ the DNA helix at

replication fork to allow the resulting single strand to be

copied

POLYMERASE

polymerises nucleotide triphosphate in a ‘5’ to ‘3’

direction. The enzyme synthesise RNA primase to act

as a template for future okazaki fragment to build on

to.

in charge of synthesizing

nucleotides onto the leading end in the classic ‘5’ to ‘3’

direction.

Synthesizing nucleotides onto primers on the

lagging strand, forming Okazaki fragment

DNA POLYMERASE I

“gluing” together Okazaki fragment on area that DNA polymerase l is

unable to synthesize

LIGASE

• Holding the replication fork of DNA open while

polymerases read the templates and

prepare for synthesizing

SINGLE STRANDED BINDING PROTEIN (SSBP)