DNA- replicación - transcripción

Figure 29.2

Griffith’s Transformation ExperimentGriffith’s Transformation Experiment19281928

Hershey–Chase Hershey–Chase ExperimentsExperiments

19521952

AnimalsHuman Homo sapiens 46Chimpanzee Pan troglodytes 48Gorilla 48Orangutan Pongo x 48Rhesus Monkey 42Mouse Mus musculus 40Cat Felis catus 38Pig 38Wolf Canis lupus 78Dog Canis lupus familiaris 78Cow Bos primigenius 60Goat 60

PlantsOats Avena sativa 42Mango Mangifera indica 40Rice Oryza sativa 24Bean Phaseolus sp. 22Wheat Triticum aestivum 42

DNA DNA NucleotidesNucleotides

James Watson, Francis Crick, and Maurice Wilkins received a Nobel Prize for the double-helix model of DNA in 1962, four years after Rosalind Franklin's death at age 37 from ovarian cancer.

DNA MoleculeDNA Molecule

A-DNAconformation B-DNA conformation Z-DNA conformation

Chromosome StructureChromosome Structure

Figure 3.14

Fig. 10-2, p. 213

Fig. 10-4, p. 214

Fig. 10-3, p. 213

DNA

Scaffolding proteins

2 μm

DNA ReplicationDNA Replication

Fig. 12-7a, p. 268

(a) Hypothesis 1: Semiconservative replication

Parental DNA First generation Second generation

Replication in Bacteria and Replication in Bacteria and EukaryotesEukaryotes

DNA SynthesisDNA Synthesis

Bidirectional ReplicationBidirectional Replication

DNASíntesis

E. coli DNA polymerase III

Fig. 12-15a, p. 276

DNA replication

RNA primer RNA primer

Removal of primer

3’

3’

3’

3’

3’

3’ 3’

3’

3’

3’

5’

5’

5’

5’5’

5’

5’

5’5’

5’

+

+

TelomeresTelomeres

Miles depurinaciones diarias

100 deaminaciones diarias

Fig. 13-4, p. 283

Nontemplate strand

TranscriptionDNA

Template strandmRNA

(complementary copy of

template DNA strand)

Codon 1 Codon 2 Codon 3 Codon 4 Codon 5 Codon 6

Polypeptide Met Thr Cys Glu Cys Phe

Translation

‘

‘

‘

‘

‘

‘

electrophoretic mobilityshift assay

enzima de restricción Hpa II

Transcripción

Antibody DiversityAntibody Diversity