DNA Repair The Ultimate Antiaging Tool By: LifeSpan International, Inc.
Mar 29, 2015
DNA RepairThe Ultimate Antiaging Tool
By: LifeSpan International, Inc. www.lifespaninternational.com
DNA IN THE “NEWS”DNA IN THE “NEWS”
On The Pill: Inner and Outer BeautyProducts; NY Post – March 6, 2005
The Science of Staying Young, Why We Age; Scientific American, Vol.14 # 3
CSI TV Program Making DNA Info Relevant
Diet & Genes, The New Science of Nutrition and Aging; Newsweek – February 7, 2005
DNA IN THE “NEWS”DNA IN THE “NEWS”
Clean Living…Dietary Supplements... the Future of Health Care: Forbes - November 29, 2004
How To Live To Be 100 (And Not Regret It); Time – August 30, 2004
A Four Year Study Measured Significant DNA Damage in Human and Animal Cells from Mobile Phone use; EU 2004
The Today Show, NBC National; “Stress Study”December 1, 2004 ( Dr. Sheldon Cohen /Elissa Epel) - University of California at San Francisco
NIH: DNA Damage Causes NIH: DNA Damage Causes AgingAging
Mutations
Replication errors
Persistent DNA damage
Genomicinstability
National Institutes of Health
Human Genome ProjectHuman Genome Project
Molecular Medicine
Antiaging Medicine
What is Aging?What is Aging?
“. . . the accumulation of random damage to the building blocks of life - especially to DNA, certain proteins, carbohydrates and lipids (fats) – that begins early in life and eventually exceeds the body’s self repair capabilities”
Leonard Hayflick, PhD., UCSF
S. Jay Olshanky, PhD., UICBruce Carnes, PhD., UIC
Scientific American, June 2002; 286(6):92-95
Why Do We Age?Why Do We Age?
Oxidative Stress
Damage to DNA (nucleus & mitochondria)
Poor DNA Repair
Aging and Aging Conditions
DNA Damage – Health DNA Damage – Health ConsequencesConsequences
DNA DAMAGEDNA DAMAGE
Mutagenic Diseases
e.g. cancer
Mutagenic Diseases
e.g. cancer
Toxic Disease
e.g. Inflammation
Toxic Disease
e.g. Inflammation
Two possible health consequences
The major cause of human disease has been identified as DNA damage by reactive oxygen species. This has been reviewed as a contributing factor for the origin and progression of a number of conditions including aging, autoimmunity, HIV infection and cancer.
Cellular Control of DNA RepairCellular Control of DNA Repair
DNA repair of damageDNA repair of damage
No diseaseNo disease
Mutagenic and toxic disease
Mutagenic and toxic disease
Incomplete Complete
Gene Deficiency
Biochemical Inhibition
Review of the cellular control of DNA damage
Results of Poor Results of Poor DNA RepairDNA Repair
DNA (gene) chemistry
Double stranded helical polymer
DNA damage caused by toxins, diet,
environment, radiation
Repair enzymes / Protein Processes
PARP, endonuclease, exonuclease, polymerase,
ligase
GOOD repair
BAD repair
GOOD
No disease
BAD
BAD REPAIR CASUES ALTERED GENE EXPRESSION, LEADS TO
CHRONIC DISEASES
Cancer, autoimmune, aging cardiovascular, inflammatory
BAD REPAIR CASUES ALTERED GENE EXPRESSION, LEADS TO
CHRONIC DISEASES
Cancer, autoimmune, aging cardiovascular, inflammatory
AC-11AC-11®®
INTRODUCING A “NEW” CLASS
OF
DIETARY SUPPLEMENTS
7 STEPS IN AGING7 STEPS IN AGING
YOUR ENVIRONMENT
(Poor diet, stress, pollution, smoking, exercise, sun exposure generate free radicals)
OXIDATIVE STRESS
GOOD GENE REPAIR
equals Antiaging
GENE DAMAGE
equals Aging
ALTERED GENE
EXPRESSION
Hobbled Proteins
(Glycation)
Wrong soup mix
(Mitochonria function)
Misfiring genetic switches
(Methylation and acetylation)
Immune glitches
(Inflammation)
Faulty cell communication
(Hormone imbalance)
Mitochondrial DNAMitochondrial DNA
A single ATP molecule must be recycled within a mitochondrion approximately
1,000 times per day for the body to maintain its energy supply
Quenching Reactive Oxygen Quenching Reactive Oxygen Species in the MitochondrionSpecies in the Mitochondrion
About 10% of the air we breathe is converted into free radicals in the mitochondria of our cells to produce the energy of life (ATP).
Hydrogen peroxide, hydroxyl radical, superoxide, lipid peroxide and singlet oxygen are metabolic mistakes of ATP production.
Mitochondria -Primary source of free radical production in cells.
AC-11®AC-11®
Water soluble extract of Uncaria tomentosa; 8% CAE’s*, less than 0.05% oxindole alkaloids
*Carboxy alkyl esters
AC-11® primary bioactive ingredient has AC-11® primary bioactive ingredient has been identifiedbeen identified Found in Shayman natural extractions of UncariaFound in Shayman natural extractions of UncariaQuinic acid/Quinic acid lactones are significantly more Quinic acid/Quinic acid lactones are significantly more bioactive when purified from AC-11®bioactive when purified from AC-11®
Aging Effects of Oxidative StressAging Effects of Oxidative Stress
Oxidative stress
Reduces apoptosis
Increases cell survival
Increases TNFalpha
Inflammation
anti-infection
NF-kB
Tumor toxicity
Decreases cell survivalal
Increases apoptosis
Decreases TNFalpha
Anti-inflammation
Enhances immune cells Increases
differentiation/function
Anti-oxidation
Increases DNA repair
Decreases DNA damage
Enhances immune cellsPro-oxidant
Increases DNA damage
Inhibits immune cells
ActivationInhibition AC-11™
Enhance DNA RepairEnhance DNA Repair
STEP 1.. DNA damage detected. Enzymes uncoil and unzip DNA. (NFk-B inhibition works here also by stopping free radicals that damage DNA)
STEP 2.. Uncoiled structure gives access to repair enzymes.
STEP 3.. Damaged or misplaced nucleotides are cut out by enzymes. (Active genes are repaired first, then less active genes)
STEP 4. New segments are moved into place and the seams are sealed by other enzymes.
STEP 1.. DNA damage detected. Enzymes uncoil and unzip DNA. (NFk-B inhibition works here also by stopping free radicals that damage DNA)
STEP 2.. Uncoiled structure gives access to repair enzymes.
STEP 3.. Damaged or misplaced nucleotides are cut out by enzymes. (Active genes are repaired first, then less active genes)
STEP 4. New segments are moved into place and the seams are sealed by other enzymes.
#4
#1
##2#2
#3
NF-kB / DNA repair-- control NF-kB / DNA repair-- control DNA damage and your health DNA damage and your health
Second primary source of free radicals is inflammation
NF-kB inhibition reduces this source
Regardless the source, DNA repair removes DNA damage
These processes are controlled by dietary factors
About AC-11®About AC-11®
Patented extract from the bark of Uncaria tomentosa vine
Patented extraction method that isolates the potent Carboxy Alkyl Esters which comprise the active ingredients in the bark
No high molecular weight alkaloids associated with adverse reactions
Sustainable Growth Project which is controlled by the Brazilian Environmental Institute (IBAMA)
Work projects are conducted under the Floravida
Institutes guidance
Unique FormulationUnique FormulationAC-11®AC-11® OTHER UT FORMULATIONSOTHER UT FORMULATIONS
Patented for DNA Repair and Immune Enhancement
Patented Aqueous Extraction Process
Inner Bark Uncaria tomentosa Water Soluble Carboxy Alkyl Esters Alkaloid Free Ethical Harvesting Produced under Pharma
GMP’s Proven Safety / Efficacy
Hydroalcoholic Tinctures, Decoctions and Solvent Extracts
Bark, Leaves &/or Root Uncaria guianensis Water Insoluble Tetracyclic Oxindole
Alkaloids Petracyclic Oxindole
Alkaloids Environmentally
Threatening Questionable Safety
Stability and Safety
Stability data > 5 years LD50 > 8000 mg/kg
– Our current recommended oral dosage is 350mg per day– Topical study doses range from 0.5% to 5.0% in
solution No known toxicity Stability in activity & color as an ingredient in
cosmetics Passes cosmetic micro-efficacy studies under
USP challenge test method DSHEA registered Prop 65 Compliant
AC-11® – AC-11® – DSHEA Allowed ClaimsDSHEA Allowed Claims
AC-11® helps enhance natural immunity
AC-11® helps enhance natural DNA repair process
AC-11® research validates it’s use to treat AC-11® research validates it’s use to treat DNA damage via NF-kB inhibition and DNA DNA damage via NF-kB inhibition and DNA
repair enhancementrepair enhancement
Induction of apoptosis and tumor inhibition; Anticancer Res., 18:1998
Enhanced DNA repair, immune function; J. Ethnopharm., 69:2000
Enhanced antibody response to vaccination; Phytomed., 8:2001
Human DNA repair enhancement; Phytomed., 8:2001
DNA repair enhanced by combination with niacin, carotenoids and zinc; Can. Detect Prev., 22:1998
AC-11® Research, AC-11® Research, cont’d.cont’d.
Serum thiols to estimate DNA repair; J. Antiaging Med., 3:2000
WBC elevation with no pathology or loss of immune function – proof of concept in mouse; Phytomed. , 2002
DNA repair as a primary molecular target for antiaging therapies; Antiaging Med., 2002
Anti-aging nutritional supplementwith and without AC-11; Anti-aging Med., 2002
Uncaria extract (AC-11) prolongs leukocyte survival; PhytMed., 2003
Uncaria Extract (AC-11) inhibits cell division and NF-kB activity; Interntl. Immunopharmacology, 2003
Strong IP ProtectionStrong IP Protection
AC-11® provides DNA repair from sun damage, lightens and blends skin tones
Patent issued for the extraction process of the rain forest botanical Uncaria tomentosa – This process results in a proprietary,
standardized extract.
Patent filed for a compound and method of use for AC-11®
Strong IP Protection, Strong IP Protection, continuedcontinued
Patent issued for Thiol blood test to measure repair status of DNA
Patent issued for compound and method of
use for Nicoplex™, a formulation with AC-11®, niacinamide and zinc. This synergistic combination has been proven to significantly improve DNA Repair.
AC-11® has demonstrated it’s positive benefits in both “Inner and Outer Beauty” products.
Synergistic To Nutrients AC-Synergistic To Nutrients AC-11®11®
Niacin (niacinamide): a substrate for DNA repair enzymes
Zinc: attaches to DNA repair enzymes, activating them
Carotenoids: scavenge oxy-radicals and enhance immune response
Edible mushroom extracts
Combination Products w/ AC-11®Combination Products w/ AC-11®i.e. i.e. Nicomed & NicoplexNicomed & Nicoplex
Synergistic Blends that Combine
AC-11® with Nutrients / AntioxidantsPatented Products
--------------------------------------------------------Age Manager – Multi-Vitamin Type with
AC-11®AGE Erasers
Scientific ValidationScientific Validation
Clinical Study using orally administered AC-11® *
-60
-50
-40
-30
-20
-10
0
10
20
DNADAMAGE2
FREE RADICALDAMAGE1
DNA REPAIRENZYMES3
DOWN40%
UP 12%
DOWN51%
Summary Summary 5 Categories of AC-11® Activity 5 Categories of AC-11® Activity
DNA Repair
Anti-inflammatory
Tumor Inhibition
Immune Enhancement
Antioxidant
OPPORTUNITIESOPPORTUNITIES
Food Beverage / Water Dietary Supplements Cosmetics / Topical Skin Care Sports Nutrition Age Management / DNA Health Care---------------------------------------------------------------------------------------------------------------
Home Based Business Network Sales Affiliate Partnerships
DNA RepairThe Ultimate Antiaging Tool
by: LifeSpan International, Inc.
www.lifespaninternational.com