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PRESENTATIONPresented by:
Shan-Salah-ud-Din 40
Rao-Abdul-Rahman 37
Ammad-Shahid 44
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Presented to:
Maam Fatima Ijaz
Presentation Topic:
Recombinant DNA TECH.
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What is recombination?
The exchangeofcorresponding DNAsegments between
adjacentchromosomes duringthe special type of
cell division thatresults in theproduction of new
genetic make up... 3
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What Is Recombinant DNA?Recombinant DNA is a form of DNA thatdoes not exist naturally, which is createdby combining DNA sequences that wouldnot normally occur together.In terms of genetic modification,recombinant DNA (rDNA) is introduced
through the addition of relevant DNA intoan existing organismal DNA.
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History of recombinant DNAtechnology
Recombinant DNA technology is one
of the recent advances inbiotechnology, which was developedby two scientists named Boyer and
Cohen in 1973.
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by natural and artificial processes, for practicalPurposes.
Intentionally modifying genomes of organisms,
Three Goals of Recombinant DNA Technology:
1. Eliminate undesirable phenotypic traits in humans, animals, plants
and microbes.2. Combine beneficial traits of two or more organisms to createvaluable new organisms.
3. Create organisms that synthesize products of humans need.
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DNA recombinant
technology
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DNA Recombinant Technology
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Basic principle of recombinantDNA technology
The DNA is inserted into another
DNA molecule called vector.
. The recombinant vector is thenintroduced into a host cell where itreplicates itself, the gene is thenproduced
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Objective:
Isolation of multiple copies of a desired gene andtransfer it to suitable organism to achieve: Large scale production of protein encoded by the gene.
Know the basics ofgene structure, function and regulation.
Be familiar with the basic methods of molecular genetics. Understand the meaning of DNA sequence and amino acid
polymorphisms.
The main purpose of rDNA technology is to make large
amounts of DNA (gene) &/or its Proteins. (Ex: Insulin).
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Steps involve in preparation of Recombinant DNA..Identification of desired Gene.
Isolation of Foreign DNA.
Formation of Chimeric
DNA.Insertion of Chimeric DNA in host Cell.
Selection of recombinant DNA.
Gene Cloning.
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Recombinant DNA Process Example:
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Applications ofRecombinant DNA
TechnologyLarge-scale production of humanproteins by genetically
engineered bacteria.
Such as : insulin, Growth
hormone, Interferon's.
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Vaccines productionFirstly, the gene in apathogenic virus that
stimulates protective immunityshould be identified.
That portion of DNA is thenisolated and incorporated intoan established harmless virus(e.g. vaccinia virus).
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This new recombinant virus isused as a vaccine.
These vaccines are muchsafer since they do not
expose the patients to theactual virus and do not riskto infection.
This method may be useful invaccines against malaria andmany viruses.
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Agricultural applicationsFrost resistant crops
Insecticide resistant crops
Herbicide resistant crops
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recombinant technology
It is always possible that anantibiotic-resistant plasmid could be
accidentally incorporated into adangerous pathogen with seriousmedical consequences.
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