diyanaAbdullah BMY3101 e-portfolio

I am diyana Bsc. (Hons) Microbiology

“ Let’s go black and

white…….and pink

“

172878

C O N T E N T

BIOGRAPHY

MY THOUGHT

TOOLS MEMOIR

MY ADOPTED MICROB PROJECT

SUMMARY

COURSE SYSNOPSIS

B I O G R A P H Y

Nurdiyana Binti Abdullah • 28 July 1992 • 15’s college • Kuala Pilah, Negeri Sembilan • love Microbiology & Biochemistry, love photography & design •

from diploma in Medical Laboratory Technology •

Course synopsis

In this course, BMY3101, I learn about the basic in microbiology. The first topic is the introduction to microbiolgy, studying microorganism through microscopic technique, prokaryotic and eukaryotic cells, non-cell microorganism, sterilization and disinfection, antimicrobial chemotherapy, microbial nutrition and metabolism, microbial genetic and lastly epidemiology and public health. This course is taught by Dr wan zuhainis bt Saad, and Prof dr. raja Noor Zaliha.

In this topic, I learn about the major

group of organism studied in microbi-ology such as bacteria, algae, fungi, protozoa and virus and their taxonomy. Besides that, I now know who the father of Microbiology is. His name is Antonie van Leeuwenhoek. Without his discovery, we might not know the small creatures that live together with us. Not just him, so many scientists had discovered and study the theory involving lives. I’m so interested to know about the golden age of microbiology, I feel so proud on how much the microbiology field has con-tribute to this world societies for example, the production of vaccines has save many lives since that time.

INTRODUCTION TO MICROBIOLOGY

INTRODUCTION TO MICROBIOLOGY

This topic brought me into a new di-

mension of microscopic technique where

I learn that so many microscopes have

their own unique function. For example,

Scanning Electron Microscopy, we can

view the surface of the cell in 3D that is

in my opinion, it is so amazing. I wish

one day I will use this kind of microscope

in my research. Not only that, in this

topic, I also learned about the slide

preparation which is going to be observe

under the microscope. Especially for light

microscope, the staining is very crucial.

From the staining we can classified the

bacteria into two group, gram positive

and gram negative bacteria. This kind

classification for me is really important

because we can identify the bacteria eas-

ily if we know its classification.

STUDYING MICROORGANISM THROUGH MICROSCOPY TECHNIQUE

STUDYING MICROORGANISM THROUGH MICROSCOPY TECHNIQUE

This topic defines me what prokaryotic cell

is. Firstly, I am able to describe the structure of

the cells, the shape and the arrangements. From

my opinion, this topic is quite connected with

previous topic, because, after the slide prepara-

tion, the staining and observation under the

microscope leads to the discovery of how the

cell looks like. Whether its shapes (cocci and

bacilli) or its arrangement (streptococci, in

chain, or staphylococci, in clusters). This topic

also focusing on the cell structure. It is impor-

tant to have the knowledge to differentiate be-

tween the structure of prokaryotic and

eukaryotic cell. The extracellular structure

includes the cell wall and cell membranes that is

from my point of view, it is very important to the

protection of cell itself and for the intracellular,

it is important to drives the function of the cells

and to carry on their lives.

ORGANIZATION & STRUCTURE OF PROKARYOTIC CELLS

ORGANIZATION & STRUCTURE OF PROKARYOTIC CELLS

After the study of prokaryotic cells, it is important to study

about eukaryotic cell also. Not only human and animal, but in the

study of microorganism, some of its group of organism falls into

this specification. They are fungi and protist. This kind of cells

carried out complex function and not only that, they having more

organelles inside their cells to carry out those complex functions.

In this topic, I have the bird-eye-view on protozoa, fungi and vi-

rus. They are called as non-cell microorganism because they fall

into the category of eukaryotic cells which means they carry vari-

ous functions and to be easy, they are protista. Protozoa is animal

-like protists and belongs to group of Mastigophorans, Amebozoa,

Apicomplexan and Ciliates. Fungi, it resembles the plant, but it is

heteretroph and many of them are saprophyte, differ with plant,

fungi digest dead organic matter and organic waste. Fungi plays

important role in ecosystem, as we know, it is a decomposers.

Then, in the context of fungi, I also learn about lichens and algae.

Lichen is an association of a fungus and photosynthetic symbiont

resulting in a stable thallus. From what I learn, Lichen acts as a

primary succession in the ecological role. They make abiotic

world habitable to biotic world. The algae also resemble plant;

they have chlorophyll that means they are photoautotropic. The

simplest example that we can see is seaweed. Not only providing

the habitat to marine communities, it is also a human food. Did

you ever heard about seaweed crackers and sushi?

EUKARYOTIC CELLS

EUKARYOTIC CELLS

When it’s come to the word

non-cell, I would expect the

non-living thing. What is the

amazing thing about the non-living

in the context of microorganism; it

is exactly a virus, viriod and prions.

They are non-living, but they have

either DNA or RNA which means

they are able to replicate. Prions on

the other hand, really shock me

because, they protein but don’t have

nucleic acid. To study the virus,

another technique of isolation must

be learning. It is because they are

obligate intracellular parasites,

which means they need live cell in

order to live.

NON-CELL MICROORGANISM

NON-CELL MICROORGANISM

For a microbiologist, the ster-

ilization and disinfection are really

important to them. Whether it

means to kill the organism or to

grow them. When we are going to

grow a certain bacteria on the

media for example, it is important

to disinfect and to sterile the wire

loop or any equipment needed to

prevent the contamination. Same

goes to killing the microbes; we

sure don’t want any of them to fol-

low us to home and having fun

with our family.

STERILIZATION & DISINFECTION

STERILIZATION & DISINFECTION

For me, antibiotics are another

way of killing microbes especially the

pathogenic one. But recently, many of

the bacteria have developed resistance

to it. I think it is important for me, as a

future microbiologist to find a new

antibiotic. The most shocking news is

there is a microbe that already resis-

tance to the antibiotic of last resort

which is vancomycin. From now on, I

would advise any of my family

members and friends to take

antibiotics following the medical

prescription. Because for me, I think

bacteria develop this kind of resistance

is due to misuse of antibiotic itself.

ANTIBIOTICS

ANTIBIOTICS

In order to identify a bacterium, it

is important for us to grow and

isolates the desired bacteria first. In

this topic, I learned how to culture

the bacteria, using a few culturing

technique such as plate culture or

broth culture. I also learned the

types of growth media that is needed

for a certain bacteria to grow. After

obtaining the single colony, then we

can do further testing for the

identification of the bacterial species.

CULTURING TECHNIQUES, ISOLATION OF BACTERIA AND TYPES OF GROWTH MEDIA

CULTURING TECHNIQUES, ISOLATION OF BACTERIA AND TYPES OF GROWTH MEDIA

In this topic, I learn about the

metabolism of bacteria. I’m so inter-

ested when in the notes; Dr. said

there is there is some cycling of

elements in ecosystem that can only

be done by microbes such as organic

nitrogen cycle. The metabolism also

affected by enzymatic reaction factor.

Enzyme has really important

function in our body and carried out

crucial role in metabolism. I also

learn the metabolism pathway, ATP

generating mechanism and even

defining the metabolism meaning.

MICROBIAL METABOLISM

MICROBIAL METABOLISM

Nutrition to a microorganism

involves two goals that are source of

carbon and source of energy. In these

topics I learn about the nutritional

requirement for the microorganism.

Some of them are photoautotropic,

photoheterotroph, chemoautotroph

and chemoheterotroph. It is important

to know the definition of each of the

nutritional requirement because it

defines what exactly the microorgan-

ism need is.

MICROBIAL NUTRITION

MICROBIAL NUTRITION

This second last topic is taught by

Prof Dr. Raja. This topic is quite tough

for me because it require a lot of

understanding rather than memorizing.

The genetic topic is really important in

microbiology course because there are a

lot of thing that we going to research is

definitely involving the genetics.

Without genetic it is impossible to truly

identify a new species of microbe and

the resistance pattern of the microbes

toward antibiotic.

INTRODUCTION TO MICROBIAL GENETICS

INTRODUCTION TO MICROBIAL GENETICS

Public health is the main

concern to any of the

microbiologist. In this topic I

learned about the spread of the

disease to human, whether it is

connected to pathogenic bacteria,

virus or fungus. It is so crucial to

study the epidemiology of

microbes in order to prevent its

spread, to control the effect of the

microbes to the community and to

give the awareness to the public in

preventing the spreading of germs.

MICROBIAL EPIDEMIOLOGY & PUBLIC

MICROBIAL EPIDEMIOLOGY & PUBLIC

memoirs

memoirs

TOOLS INFO

MY ADOPT A MICROB PROJECT

My thoughts….

“ after taking this course, I know the basic of

microbiology and I thinks Dr Wan is very

passionate people. I believe that she had

successfully made everyone love

microbiology so much, she also brings

learning process into a new level. I taught on

how to do assignment, quizzes, exploring

microbiology via internet”